{"id":"https://openalex.org/W2062979470","doi":"https://doi.org/10.1016/0022-1759(92)90126-e","title":"A novel B cell stimulation/proliferation assay using simultaneous flow cytometric detection of cell surface markers and DNA content","display_name":"A novel B cell stimulation/proliferation assay using simultaneous flow cytometric detection of cell surface markers and DNA content","publication_year":1992,"publication_date":"1992-07-01","ids":{"openalex":"https://openalex.org/W2062979470","doi":"https://doi.org/10.1016/0022-1759(92)90126-e","mag":"2062979470","pmid":"https://pubmed.ncbi.nlm.nih.gov/1629615"},"language":"en","primary_location":{"id":"doi:10.1016/0022-1759(92)90126-e","doi":"https://doi.org/10.1016/0022-1759(92)90126-e","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0022-1759(92)90126-e","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084879148","display_name":"Jan Storek","orcid":"https://orcid.org/0000-0002-7884-9122","observed_orcids":["https://orcid.org/0000-0002-7884-9122"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jan Storek","raw_affiliation_strings":["Hart and Louise Lyon Laboratory, Department of Medicine, UCLA School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hart and Louise Lyon Laboratory, Department of Medicine, UCLA School of Medicine","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111688355","display_name":"Ingrid Schmid","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ingrid Schmid","raw_affiliation_strings":["Division of Clinical Immunology/ Allergy, Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90024-1680, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Clinical Immunology/ Allergy, Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90024-1680, USA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027175258","display_name":"Stephen A. Ferrara","orcid":"https://orcid.org/0000-0003-2287-8255","observed_orcids":["https://orcid.org/0000-0003-2287-8255"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen Ferrara","raw_affiliation_strings":["Division of Cellular Immunology and Cytometry, Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90024-1680, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Cellular Immunology and Cytometry, Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90024-1680, USA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5035371856","display_name":"Andrew Saxon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4390039293","display_name":"UCLA Jonsson Comprehensive Cancer Center","ror":"https://ror.org/0599cs764","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799798094","https://openalex.org/I4390039293"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew Saxon","raw_affiliation_strings":["The Jonsson Comprehensive Cancer Center Flow Cytometry Laboratory, Los Angeles, CA 90024-1680, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Jonsson Comprehensive Cancer Center Flow Cytometry Laboratory, Los Angeles, CA 90024-1680, USA","institution_ids":["https://openalex.org/I4390039293"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5084879148"],"corresponding_institution_ids":["https://openalex.org/I161318765"],"apc_list":{"value":2970,"currency":"USD","value_usd":2970},"apc_paid":null,"fwci":0.1611,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.45584894,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"151","issue":"1-2","first_page":"261","last_page":"267"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/b-cell-proliferation","display_name":"B cell proliferation","score":0.7789999842643738},{"id":"https://openalex.org/keywords/b-cell-activation","display_name":"B cell activation","score":0.8500000238418579},{"id":"https://openalex.org/keywords/s-and-g2-m-phases","display_name":"S/G2/M phase","score":0.6880000233650208},{"id":"https://openalex.org/keywords/bone-marrow-transplant-recipients","display_name":"bone marrow transplant recipients","score":0.8970000147819519},{"id":"https://openalex.org/keywords/flow-cytometry-assay","display_name":"flow cytometry assay","score":0.6240000128746033}],"concepts":[{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.7413001656532288},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6421129703521729},{"id":"https://openalex.org/C2778957590","wikidata":"https://www.wikidata.org/wiki/Q798033","display_name":"CD19","level":3,"score":0.6313688158988953},{"id":"https://openalex.org/C2778453870","wikidata":"https://www.wikidata.org/wiki/Q188930","display_name":"B cell","level":3,"score":0.6211516261100769},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5972902178764343},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.5355588793754578},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.5269399881362915},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5107729434967041},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.49408987164497375},{"id":"https://openalex.org/C18031839","wikidata":"https://www.wikidata.org/wiki/Q5009796","display_name":"CD20","level":3,"score":0.4538128077983856},{"id":"https://openalex.org/C2781005381","wikidata":"https://www.wikidata.org/wiki/Q7208633","display_name":"Pokeweed mitogen","level":4,"score":0.41119909286499023},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33737748861312866},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3335871696472168},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3311837315559387},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.2155803143978119},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2075691521167755},{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.18061122298240662},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.12092357873916626},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.11739164590835571}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000944","descriptor_name":"Antigens, Differentiation, B-Lymphocyte","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001402","descriptor_name":"B-Lymphocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001402","descriptor_name":"B-Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D002453","descriptor_name":"Cell Cycle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007075","descriptor_name":"Immunoglobulin M","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011947","descriptor_name":"Receptors, Antigen, B-Cell","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0022-1759(92)90126-e","doi":"https://doi.org/10.1016/0022-1759(92)90126-e","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0022-1759(92)90126-e","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},{"id":"pmid:1629615","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1629615","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W1917147955","https://openalex.org/W1981256004","https://openalex.org/W1982297359","https://openalex.org/W2017753207","https://openalex.org/W2030869490","https://openalex.org/W2161620889","https://openalex.org/W2417849265"],"related_works":["https://openalex.org/W312864289","https://openalex.org/W2048508297","https://openalex.org/W2006572581","https://openalex.org/W2054050364","https://openalex.org/W2047402242","https://openalex.org/W2113065280","https://openalex.org/W1643633231","https://openalex.org/W1962405670","https://openalex.org/W2417556081","https://openalex.org/W2034571018"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}