{"id":"https://openalex.org/W2062952025","doi":"https://doi.org/10.1055/s-0030-1249773","title":"High Frequency Plant Regeneration from Leaf Derived Callus of High\u03949-Tetrahydrocannabinol YieldingCannabis sativaL.","display_name":"High Frequency Plant Regeneration from Leaf Derived Callus of High\u03949-Tetrahydrocannabinol YieldingCannabis sativaL.","publication_year":2010,"publication_date":"2010-03-30","ids":{"openalex":"https://openalex.org/W2062952025","doi":"https://doi.org/10.1055/s-0030-1249773","mag":"2062952025","pmid":"https://pubmed.ncbi.nlm.nih.gov/20354950"},"language":"en","primary_location":{"id":"doi:10.1055/s-0030-1249773","doi":"https://doi.org/10.1055/s-0030-1249773","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0030-1249773","pdf_url":null,"source":{"id":"https://openalex.org/S39653451","display_name":"Planta Medica","issn_l":"0032-0943","issn":["0032-0943","1439-0221"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Planta Medica","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110958610","display_name":"Hemant Lata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I368840534","display_name":"University of Mississippi","ror":"https://ror.org/02teq1165","country_code":"US","type":"education","lineage":["https://openalex.org/I368840534","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hemant Lata","raw_affiliation_strings":["School of Pharmacy, University of Mississippi, University, MS, USA. hlata@olemiss.edu","National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmacy, University of Mississippi, University, MS, USA. hlata@olemiss.edu","institution_ids":["https://openalex.org/I368840534"]},{"raw_affiliation_string":"National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA","institution_ids":["https://openalex.org/I368840534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001064903","display_name":"Suman Chandra","orcid":"https://orcid.org/0000-0003-1991-9466","observed_orcids":["https://orcid.org/0000-0003-1991-9466"]},"institutions":[{"id":"https://openalex.org/I368840534","display_name":"University of Mississippi","ror":"https://ror.org/02teq1165","country_code":"US","type":"education","lineage":["https://openalex.org/I368840534","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Suman Chandra","raw_affiliation_strings":["National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA","institution_ids":["https://openalex.org/I368840534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049082763","display_name":"Ikhlas Ahmed Khan","orcid":"https://orcid.org/0000-0001-5464-4643","observed_orcids":["https://orcid.org/0000-0001-5464-4643"]},"institutions":[{"id":"https://openalex.org/I368840534","display_name":"University of Mississippi","ror":"https://ror.org/02teq1165","country_code":"US","type":"education","lineage":["https://openalex.org/I368840534","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ikhlas Khan","raw_affiliation_strings":["Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS, USA","National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS, USA","institution_ids":["https://openalex.org/I368840534"]},{"raw_affiliation_string":"National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA","institution_ids":["https://openalex.org/I368840534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046094471","display_name":"Mahmoud A. ElSohly","orcid":"https://orcid.org/0000-0002-0019-2001","observed_orcids":["https://orcid.org/0000-0002-0019-2001"]},"institutions":[{"id":"https://openalex.org/I368840534","display_name":"University of Mississippi","ror":"https://ror.org/02teq1165","country_code":"US","type":"education","lineage":["https://openalex.org/I368840534","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mahmoud ElSohly","raw_affiliation_strings":["Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS, USA","National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS, USA","institution_ids":["https://openalex.org/I368840534"]},{"raw_affiliation_string":"National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS, USA","institution_ids":["https://openalex.org/I368840534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I368840534"],"apc_list":null,"apc_paid":null,"fwci":1.1365,"has_fulltext":false,"cited_by_count":87,"citation_normalized_percentile":{"value":0.78998752,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"76","issue":"14","first_page":"1629","last_page":"1633"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12113","display_name":"Growth and nutrition in plants","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cannabis-sativa","display_name":"Cannabis sativa","score":0.9959999918937683},{"id":"https://openalex.org/keywords/leaf-derived-callus","display_name":"leaf-derived callus","score":0.7870000004768372},{"id":"https://openalex.org/keywords/micropropagation","display_name":"micropropagation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/in-vitro-plant-regeneration","display_name":"in vitro plant regeneration","score":0.7570000290870667},{"id":"https://openalex.org/keywords/thidiazuron","display_name":"thidiazuron","score":0.8130000233650208},{"id":"https://openalex.org/keywords/cytokinins","display_name":"cytokinins","score":0.8109999895095825},{"id":"https://openalex.org/keywords/auxin","display_name":"auxin","score":0.8330000042915344},{"id":"https://openalex.org/keywords/shoot-induction","display_name":"shoot induction","score":0.6299999952316284},{"id":"https://openalex.org/keywords/root-induction","display_name":"root induction","score":0.6290000081062317},{"id":"https://openalex.org/keywords/plantlet-acclimatization","display_name":"plantlet acclimatization","score":0.6340000033378601}],"concepts":[{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.7907898426055908},{"id":"https://openalex.org/C207778908","wikidata":"https://www.wikidata.org/wiki/Q190022","display_name":"Auxin","level":3,"score":0.6826357245445251},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.6704624891281128},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.6217004060745239},{"id":"https://openalex.org/C2776673659","wikidata":"https://www.wikidata.org/wiki/Q47512","display_name":"Acetic acid","level":2,"score":0.5928726196289062},{"id":"https://openalex.org/C2780822416","wikidata":"https://www.wikidata.org/wiki/Q1934105","display_name":"Micropropagation","level":4,"score":0.5909717082977295},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.5039119124412537},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.47936493158340454},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.46434956789016724},{"id":"https://openalex.org/C2779843069","wikidata":"https://www.wikidata.org/wiki/Q193213","display_name":"Butyric acid","level":2,"score":0.45452937483787537},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4170018434524536},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3981497883796692},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.231248676776886},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.1246868371963501},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.05555209517478943},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002188","descriptor_name":"Cannabis","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D002188","descriptor_name":"Cannabis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002188","descriptor_name":"Cannabis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D012038","descriptor_name":"Regeneration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013759","descriptor_name":"Dronabinol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018520","descriptor_name":"Plant Shoots","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D018520","descriptor_name":"Plant Shoots","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018520","descriptor_name":"Plant Shoots","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D019976","descriptor_name":"Cloning, Organism","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D046509","descriptor_name":"Tissue Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1055/s-0030-1249773","doi":"https://doi.org/10.1055/s-0030-1249773","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0030-1249773","pdf_url":null,"source":{"id":"https://openalex.org/S39653451","display_name":"Planta Medica","issn_l":"0032-0943","issn":["0032-0943","1439-0221"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Planta Medica","raw_type":"journal-article"},{"id":"pmid:20354950","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20354950","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Planta medica","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G912414987","display_name":null,"funder_award_id":"N01DA-7-7746","funder_id":"https://openalex.org/F4320337347","funder_display_name":"National Institute on Drug Abuse"}],"funders":[{"id":"https://openalex.org/F4320337347","display_name":"National Institute on Drug Abuse","ror":"https://ror.org/00fq5cm18"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4406024505","https://openalex.org/W6944316253","https://openalex.org/W2323562852","https://openalex.org/W2280499830","https://openalex.org/W3110794626","https://openalex.org/W4403524324","https://openalex.org/W4403101332","https://openalex.org/W1980599127","https://openalex.org/W7138911949","https://openalex.org/W4410590737"],"abstract_inverted_index":{"An":[0],"efficient":[1],"in":[2,86,106,139,175,185,197],"vitro":[3],"propagation":[4],"protocol":[5],"for":[6,45,94],"rapidly":[7],"producing":[8],"Cannabis":[9],"sativa":[10],"plantlets":[11,171],"from":[12,50],"young":[13],"leaf":[14,51],"tissue":[15],"was":[16,36,105,137],"developed.":[17],"Using":[18],"gas":[19],"chromatography-flame":[20],"ionization":[21],"detection":[22],"(GC-FID),":[23],"high":[24],"THC":[25],"yielding":[26],"elite":[27],"female":[28],"clone":[29],"of":[30,68,91,97,125,132,194],"a":[31,191],"drug-type":[32],"CANNABIS":[33],"variety":[34],"(MX)":[35],"screened":[37],"and":[38,55,65,82,103,135,149,177,187],"its":[39],"vegetatively":[40],"propagated":[41],"clones":[42],"were":[43,48,117,172],"used":[44],"micropropagation.":[46],"Calli":[47],"induced":[49],"explant":[52],"on":[53,156],"Murashige":[54],"Skoog":[56],"medium":[57,160],"supplemented":[58,164],"with":[59,88,165,181],"different":[60,123],"concentrations":[61,124],"(0.5,":[62],"1.0,":[63],"1.5,":[64],"2.0":[66],"\u00b5M)":[67],"indole-":[69,73],"3-acetic":[70],"acid":[71,76,80,84],"(IAA),":[72],"3-":[74],"butyric":[75],"(IBA),":[77],"naphthalene":[78],"acetic":[79],"(NAA),":[81],"2,4-dichlorophenoxy-acetic":[83],"(2,4-D)":[85],"combination":[87],"1.0":[89,111],"\u00b5M":[90,108,112,141,167],"thidiazuron":[92],"(TDZ)":[93],"the":[95,144,198],"production":[96],"callus.":[98],"The":[99,114,130,169],"optimum":[100],"callus":[101],"growth":[102],"maintenance":[104],"0.5":[107,140],"NAA":[109],"plus":[110],"TDZ.":[113,142],"two-month-old":[115],"calli":[116],"subcultured":[118],"to":[119,179],"MS":[120,159],"media":[121],"containing":[122],"cytokinins":[126],"(BAP,":[127],"KN,":[128],"TDZ).":[129],"rate":[131,193],"shoot":[133],"induction":[134],"proliferation":[136],"highest":[138],"Of":[143],"various":[145],"auxins":[146],"(IAA,":[147],"IBA,":[148],"NAA)":[150],"tested,":[151],"regenerated":[152],"shoots":[153],"rooted":[154,170],"best":[155],"half":[157],"strength":[158],"(1/2":[161],"-":[162],"MS)":[163],"2.5":[166],"IBA.":[168],"successfully":[173],"established":[174],"soil":[176],"grown":[178],"maturity":[180],"no":[182],"gross":[183],"variations":[184],"morphology":[186],"cannabinoids":[188],"content":[189],"at":[190],"survival":[192],"95":[195],"%":[196],"indoor":[199],"growroom.":[200]},"counts_by_year":[{"year":2026,"cited_by_count":8},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":16},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":12},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":5},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}