{"id":"https://openalex.org/W2062695451","doi":"https://doi.org/10.1074/jbc.m513583200","title":"Characterization of the Functional Epitope on the Urokinase Receptor","display_name":"Characterization of the Functional Epitope on the Urokinase Receptor","publication_year":2006,"publication_date":"2006-05-04","ids":{"openalex":"https://openalex.org/W2062695451","doi":"https://doi.org/10.1074/jbc.m513583200","mag":"2062695451","pmid":"https://pubmed.ncbi.nlm.nih.gov/16672229"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m513583200","doi":"https://doi.org/10.1074/jbc.m513583200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m513583200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m513583200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002497054","display_name":"Henrik G\u00e5rdsvoll","orcid":"https://orcid.org/0000-0003-0691-3567","observed_orcids":["https://orcid.org/0000-0003-0691-3567"]},"institutions":[{"id":"https://openalex.org/I2802709434","display_name":"Rigshospitalet","ror":"https://ror.org/03mchdq19","country_code":"DK","type":"healthcare","lineage":["https://openalex.org/I2802567020","https://openalex.org/I2802709434","https://openalex.org/I4210145385"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Henrik G\u00e5rdsvoll","raw_affiliation_strings":["Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen \u00d8, Denmark","Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen O, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen \u00d8, Denmark","institution_ids":["https://openalex.org/I2802709434"]},{"raw_affiliation_string":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen O, Denmark","institution_ids":["https://openalex.org/I2802709434"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058153859","display_name":"Bernard Gilquin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128565","display_name":"CEA Paris-Saclay","ror":"https://ror.org/03n15ch10","country_code":"FR","type":"government","lineage":["https://openalex.org/I2738703131","https://openalex.org/I277688954","https://openalex.org/I4210128565"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Bernard Gilquin","raw_affiliation_strings":["De\u0301partement d'Inge\u0301nierie et d'Etudes des Prote\u0301ines, Commissariat a\u0300 l'Energie Atomique, CE Saclay, 91191 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"De\u0301partement d'Inge\u0301nierie et d'Etudes des Prote\u0301ines, Commissariat a\u0300 l'Energie Atomique, CE Saclay, 91191 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I4210128565"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012490479","display_name":"Marie\u2010H\u00e9l\u00e8ne Le Du","orcid":"https://orcid.org/0000-0002-4759-3160","observed_orcids":["https://orcid.org/0000-0002-4759-3160"]},"institutions":[{"id":"https://openalex.org/I4210128565","display_name":"CEA Paris-Saclay","ror":"https://ror.org/03n15ch10","country_code":"FR","type":"government","lineage":["https://openalex.org/I2738703131","https://openalex.org/I277688954","https://openalex.org/I4210128565"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Marie H\u00e9l\u00e8ne Le Du","raw_affiliation_strings":["De\u0301partement d'Inge\u0301nierie et d'Etudes des Prote\u0301ines, Commissariat a\u0300 l'Energie Atomique, CE Saclay, 91191 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"De\u0301partement d'Inge\u0301nierie et d'Etudes des Prote\u0301ines, Commissariat a\u0300 l'Energie Atomique, CE Saclay, 91191 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I4210128565"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112445581","display_name":"Andre M\u00e9n\u00e8z","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128565","display_name":"CEA Paris-Saclay","ror":"https://ror.org/03n15ch10","country_code":"FR","type":"government","lineage":["https://openalex.org/I2738703131","https://openalex.org/I277688954","https://openalex.org/I4210128565"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Andre M\u00e9n\u00e8z","raw_affiliation_strings":["De\u0301partement d'Inge\u0301nierie et d'Etudes des Prote\u0301ines, Commissariat a\u0300 l'Energie Atomique, CE Saclay, 91191 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"De\u0301partement d'Inge\u0301nierie et d'Etudes des Prote\u0301ines, Commissariat a\u0300 l'Energie Atomique, CE Saclay, 91191 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I4210128565"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066094184","display_name":"Thomas J. D. J\u00f8rgensen","orcid":"https://orcid.org/0000-0002-7149-316X","observed_orcids":["https://orcid.org/0000-0002-7149-316X"]},"institutions":[{"id":"https://openalex.org/I177969490","display_name":"University of Southern Denmark","ror":"https://ror.org/03yrrjy16","country_code":"DK","type":"education","lineage":["https://openalex.org/I177969490"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Thomas J.D. J\u00f8rgensen","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark","institution_ids":["https://openalex.org/I177969490"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071117617","display_name":"Michael Ploug","orcid":"https://orcid.org/0000-0003-2215-4265","observed_orcids":["https://orcid.org/0000-0003-2215-4265"]},"institutions":[{"id":"https://openalex.org/I2802709434","display_name":"Rigshospitalet","ror":"https://ror.org/03mchdq19","country_code":"DK","type":"healthcare","lineage":["https://openalex.org/I2802567020","https://openalex.org/I2802709434","https://openalex.org/I4210145385"]}],"countries":["DK"],"is_corresponding":true,"raw_author_name":"Michael Ploug","raw_affiliation_strings":["Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen \u00d8, Denmark","Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen O, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen \u00d8, Denmark","institution_ids":["https://openalex.org/I2802709434"]},{"raw_affiliation_string":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen O, Denmark","institution_ids":["https://openalex.org/I2802709434"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5071117617"],"corresponding_institution_ids":["https://openalex.org/I2802709434"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.8797,"has_fulltext":false,"cited_by_count":84,"citation_normalized_percentile":{"value":0.92048542,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"281","issue":"28","first_page":"19260","last_page":"19272"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.018400000408291817,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/urokinase-receptor","display_name":"urokinase receptor","score":0.9010000228881836},{"id":"https://openalex.org/keywords/urokinase-type-plasminogen-activator","display_name":"urokinase","score":0.9850000143051147},{"id":"https://openalex.org/keywords/functional-epitopes","display_name":"functional epitopes","score":0.8209999799728394},{"id":"https://openalex.org/keywords/alanine-scanning","display_name":"alanine scanning","score":0.9300000071525574},{"id":"https://openalex.org/keywords/cross-linking","display_name":"crosslinking","score":0.8169999718666077},{"id":"https://openalex.org/keywords/distance-constraints","display_name":"distance constraints","score":0.5910000205039978},{"id":"https://openalex.org/keywords/kringle-domains","display_name":"kringle domains","score":0.6779999732971191},{"id":"https://openalex.org/keywords/plasminogen-activation","display_name":"plasminogen activation","score":0.7400000095367432},{"id":"https://openalex.org/keywords/hydrophobic-interactions","display_name":"hydrophobic interactions","score":0.8309999704360962},{"id":"https://openalex.org/keywords/antagonist-design","display_name":"antagonist design","score":0.5220000147819519},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.42399999499320984}],"concepts":[{"id":"https://openalex.org/C56219504","wikidata":"https://www.wikidata.org/wiki/Q7900869","display_name":"Urokinase receptor","level":3,"score":0.9753832817077637},{"id":"https://openalex.org/C2780227090","wikidata":"https://www.wikidata.org/wiki/Q2636892","display_name":"Alanine scanning","level":5,"score":0.7554316520690918},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.6258618235588074},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5896393060684204},{"id":"https://openalex.org/C2779856020","wikidata":"https://www.wikidata.org/wiki/Q218642","display_name":"Alanine","level":3,"score":0.5648614168167114},{"id":"https://openalex.org/C2779679481","wikidata":"https://www.wikidata.org/wiki/Q13581432","display_name":"Plasminogen activator","level":2,"score":0.5256641507148743},{"id":"https://openalex.org/C37600318","wikidata":"https://www.wikidata.org/wiki/Q24726526","display_name":"Kringle domain","level":4,"score":0.4857783615589142},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4701554775238037},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4692625403404236},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.42888665199279785},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.41704702377319336},{"id":"https://openalex.org/C106847996","wikidata":"https://www.wikidata.org/wiki/Q898756","display_name":"Surface plasmon resonance","level":3,"score":0.4139358699321747},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.35566410422325134},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.35284656286239624},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29877063632011414},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.28354477882385254},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.24445053935050964},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23784705996513367},{"id":"https://openalex.org/C2781071845","wikidata":"https://www.wikidata.org/wiki/Q21173374","display_name":"Plasmin","level":3,"score":0.15625661611557007},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.13617926836013794},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.13333749771118164},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09850871562957764},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.09396648406982422},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C155672457","wikidata":"https://www.wikidata.org/wiki/Q61231","display_name":"Nanoparticle","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000409","descriptor_name":"Alanine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D003432","descriptor_name":"Cross-Linking Reagents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005821","descriptor_name":"Genetic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011956","descriptor_name":"Receptors, Cell Surface","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011956","descriptor_name":"Receptors, Cell Surface","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055293","descriptor_name":"Receptors, Urokinase Plasminogen Activator","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1074/jbc.m513583200","doi":"https://doi.org/10.1074/jbc.m513583200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m513583200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:16672229","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16672229","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/b3b74e35-7a7a-400b-9175-5d7a6915ce32","doi":null,"is_oa":false,"landing_page_url":"https://researchprofiles.ku.dk/da/publications/b3b74e35-7a7a-400b-9175-5d7a6915ce32","pdf_url":null,"source":{"id":"https://openalex.org/S4306401983","display_name":"Research at the University of Copenhagen (University of Copenhagen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I124055696","host_organization_name":"University of Copenhagen","host_organization_lineage":["https://openalex.org/I124055696"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"G\u00e5rdsvoll, H, Gilquin, B, Le Du, M H, M\u00e9n\u00e8z, A, J\u00f8rgensen, T J D & Ploug, M 2006, 'Characterization of the functional epitope on the urokinase receptor. Complete alanine scanning mutagenesis supplemented by chemical cross-linking', The Journal of Biological Chemistry, vol. 281, no. 28, pp. 19260-72. https://doi.org/10.1074/jbc.M513583200","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:sdu.dk:openaire_cris_publications/01df65d0-d851-11db-ae98-000ea68e967b","doi":null,"is_oa":false,"landing_page_url":"https://portal.findresearcher.sdu.dk/da/publications/01df65d0-d851-11db-ae98-000ea68e967b","pdf_url":null,"source":{"id":"https://openalex.org/S4306400423","display_name":"University of Southern Denmark Research Portal (University of Southern Denmark)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I177969490","host_organization_name":"University of Southern Denmark","host_organization_lineage":["https://openalex.org/I177969490"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"G\u00e5rdsvoll, H, Gilquin, B, Le Du, M H, M\u00e9n\u00e8z, A, J\u00f8rgensen, T J D & Ploug, M 2006, 'Characterization of the functional epitope on the urokinase receptor : Complete alanine scanning mutagenesis supplemented by chemical crosslinking', Journal of Biological Chemistry, vol. 281, no. 28, pp. 19260-19272. https://doi.org/10.1074/jbc.M513583200","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m513583200","doi":"https://doi.org/10.1074/jbc.m513583200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m513583200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":59,"referenced_works":["https://openalex.org/W1536836107","https://openalex.org/W1548496378","https://openalex.org/W1598321878","https://openalex.org/W1598733586","https://openalex.org/W1608720736","https://openalex.org/W1611413521","https://openalex.org/W1618760884","https://openalex.org/W1670322156","https://openalex.org/W1835991237","https://openalex.org/W1944789189","https://openalex.org/W1964742940","https://openalex.org/W1967156030","https://openalex.org/W1969505913","https://openalex.org/W1969821143","https://openalex.org/W1971831135","https://openalex.org/W1977451666","https://openalex.org/W1979214984","https://openalex.org/W1983191482","https://openalex.org/W1986158569","https://openalex.org/W1986388027","https://openalex.org/W1988059534","https://openalex.org/W1988244714","https://openalex.org/W1988569065","https://openalex.org/W1989161548","https://openalex.org/W2002996779","https://openalex.org/W2003593894","https://openalex.org/W2003844587","https://openalex.org/W2007109141","https://openalex.org/W2010031911","https://openalex.org/W2010695917","https://openalex.org/W2012974717","https://openalex.org/W2013166539","https://openalex.org/W2015314629","https://openalex.org/W2027040860","https://openalex.org/W2027408247","https://openalex.org/W2038182717","https://openalex.org/W2042882433","https://openalex.org/W2043213039","https://openalex.org/W2047686980","https://openalex.org/W2048362048","https://openalex.org/W2058527555","https://openalex.org/W2068662040","https://openalex.org/W2075713375","https://openalex.org/W2077288582","https://openalex.org/W2085010724","https://openalex.org/W2087312978","https://openalex.org/W2092233154","https://openalex.org/W2103307075","https://openalex.org/W2105111621","https://openalex.org/W2110274253","https://openalex.org/W2110625359","https://openalex.org/W2123631149","https://openalex.org/W2127160878","https://openalex.org/W2127543909","https://openalex.org/W2129986286","https://openalex.org/W2131307163","https://openalex.org/W2140690574","https://openalex.org/W2143415621","https://openalex.org/W2147471000"],"related_works":["https://openalex.org/W1971831135","https://openalex.org/W2060915897","https://openalex.org/W2143415621","https://openalex.org/W2027040860","https://openalex.org/W2069846304","https://openalex.org/W2075106606","https://openalex.org/W1979214984","https://openalex.org/W2026666216","https://openalex.org/W7046362257","https://openalex.org/W2064283575"],"abstract_inverted_index":{"The":[0,110,233],"high":[1],"affinity":[2],"interaction":[3,116,162,237],"between":[4,117],"the":[5,20,30,51,55,70,102,115,140,160,169,172,178,209,216,224,228,246,249,254,264],"serine":[6],"protease":[7],"urokinase-type":[8],"plasminogen":[9,27],"activator":[10],"(uPA)":[11],"and":[12,79,119,200,241,251],"its":[13],"glycolipid-anchored":[14],"receptor":[15],"(uPAR)":[16],"represents":[17],"one":[18],"of":[19,59,92,137,142,171,180,194,208,219,230,235,248,253,263],"key":[21],"regulatory":[22],"steps":[23],"in":[24,39,147,184,198,214],"cell":[25],"surface-associated":[26],"activation.":[28],"On":[29],"basis":[31],"on":[32],"our":[33],"crystal":[34],"structure":[35],"solved":[36],"for":[37,50,114,159],"uPAR":[38,93,122,138,199,210],"complex":[40,53,103,213],"with":[41,54,95,124],"a":[42,48,87,206],"peptide":[43],"antagonist,":[44],"we":[45,73],"recently":[46],"proposed":[47],"model":[49,83,207],"corresponding":[52],"growth":[56],"factor-like":[57],"domain":[58,218],"uPA":[60,161,212,220],"(Llinas":[61],"et":[62],"al.":[63],"(2005)":[64],"EMBO":[65],"J.":[66],"24,":[67],"1655-1663).":[68],"In":[69],"present":[71],"study,":[72],"provide":[74],"experimental":[75],"evidence":[76],"that":[77],"consolidates":[78],"further":[80],"develops":[81],"this":[82,148,191,236],"using":[84,104],"data":[85],"from":[86],"comprehensive":[88],"alanine":[89,135],"scanning":[90,136],"mutagenesis":[91],"combined":[94],"low":[96,259],"resolution":[97],"distance":[98],"constraints":[99,225],"defined":[100],"within":[101,166],"chemical":[105],"cross-linkers":[106],"as":[107],"molecular":[108,260],"rulers.":[109],"kinetic":[111],"rate":[112],"constants":[113],"pro-uPA":[118,201],"244":[120],"purified":[121],"mutants":[123],"single-site":[125],"replacements":[126],"were":[127],"determined":[128],"by":[129,177,223,227],"surface":[130],"plasmon":[131],"resonance.":[132],"This":[133],"complete":[134],"highlighted":[139],"involvement":[141],"20":[143],"surface-exposed":[144],"side":[145],"chains":[146],"interaction.":[149,266],"Mutations":[150],"causing":[151],"delta":[152],"deltaG":[153],">":[154],"or":[155,167],"=":[156],"1":[157],"kcal/mol":[158],"are":[163,188],"all":[164,181],"located":[165],"at":[168],"rim":[170],"central":[173,255],"cavity":[174,256],"uniquely":[175],"formed":[176],"assembly":[179],"three":[182],"domains":[183],"uPAR,":[185],"whereas":[186],"none":[187],"found":[189],"outside":[190],"crevice.":[192],"Identification":[193],"specific":[195],"cross-linking":[196],"sites":[197],"enabled":[202],"us":[203],"to":[204],"build":[205],"x":[211],"which":[215],"kringle":[217],"was":[221],"positioned":[222],"established":[226],"range":[229],"these":[231],"cross-linkers.":[232],"nature":[234],"is":[238],"predominantly":[239],"hydrophobic":[240],"highly":[242],"asymmetric,":[243],"thus":[244],"emphasizing":[245],"importance":[247],"shape":[250],"size":[252],"when":[257],"designing":[258],"mass":[261],"antagonists":[262],"uPAR/uPA":[265]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":6},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":5}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}