{"id":"https://openalex.org/W2061943641","doi":"https://doi.org/10.1074/jbc.m413995200","title":"Characterization of a Conserved C-terminal Motif (RSPRR) in Ribosomal Protein S6 Kinase 1 Required for Its Mammalian Target of Rapamycin-dependent Regulation","display_name":"Characterization of a Conserved C-terminal Motif (RSPRR) in Ribosomal Protein S6 Kinase 1 Required for Its Mammalian Target of Rapamycin-dependent Regulation","publication_year":2005,"publication_date":"2005-01-20","ids":{"openalex":"https://openalex.org/W2061943641","doi":"https://doi.org/10.1074/jbc.m413995200","mag":"2061943641","pmid":"https://pubmed.ncbi.nlm.nih.gov/15659381"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m413995200","doi":"https://doi.org/10.1074/jbc.m413995200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m413995200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m413995200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002408948","display_name":"Stefanie S. Schalm","orcid":"https://orcid.org/0009-0002-1303-3699","observed_orcids":["https://orcid.org/0009-0002-1303-3699"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stefanie S. Schalm","raw_affiliation_strings":["Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043896468","display_name":"Andrew R. Tee","orcid":"https://orcid.org/0000-0002-5577-4631","observed_orcids":["https://orcid.org/0000-0002-5577-4631"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew R. Tee","raw_affiliation_strings":["Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019076582","display_name":"John Blenis","orcid":"https://orcid.org/0000-0003-3622-2337","observed_orcids":["https://orcid.org/0000-0003-3622-2337"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John Blenis","raw_affiliation_strings":["Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5019076582"],"corresponding_institution_ids":["https://openalex.org/I136199984"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.0672,"has_fulltext":false,"cited_by_count":54,"citation_normalized_percentile":{"value":0.86277115,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"280","issue":"12","first_page":"11101","last_page":"11106"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ribosomal-protein-s6-kinase-1","display_name":"ribosomal protein S6 kinase 1","score":0.9950000047683716},{"id":"https://openalex.org/keywords/mtor","display_name":"mTOR","score":0.75},{"id":"https://openalex.org/keywords/s6k1-phosphorylation","display_name":"S6K1 phosphorylation","score":0.7979999780654907},{"id":"https://openalex.org/keywords/rapamycin-resistance","display_name":"rapamycin resistance","score":0.9160000085830688},{"id":"https://openalex.org/keywords/kinase-activation","display_name":"kinase activation","score":0.5400000214576721},{"id":"https://openalex.org/keywords/negative-regulation","display_name":"negative regulation","score":0.6349999904632568},{"id":"https://openalex.org/keywords/cell-growth-and-proliferation","display_name":"cell growth and proliferation","score":0.5979999899864197}],"concepts":[{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.5274869799613953},{"id":"https://openalex.org/C38062823","wikidata":"https://www.wikidata.org/wiki/Q3408228","display_name":"Ribosomal protein","level":5,"score":0.5206415057182312},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4920015037059784},{"id":"https://openalex.org/C2779664074","wikidata":"https://www.wikidata.org/wiki/Q3518405","display_name":"Terminal (telecommunication)","level":2,"score":0.48120978474617004},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.458352267742157},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.4309088885784149},{"id":"https://openalex.org/C32276052","wikidata":"https://www.wikidata.org/wiki/Q908349","display_name":"Motif (music)","level":2,"score":0.4280781149864197},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42137789726257324},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40432804822921753},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.35423627495765686},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3175548315048218},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.2456553876399994},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17994871735572815},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.09313789010047913},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.08152049779891968},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.07196417450904846},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017124","descriptor_name":"Conserved Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020123","descriptor_name":"Sirolimus","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D038762","descriptor_name":"Ribosomal Protein S6 Kinases, 70-kDa","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D038762","descriptor_name":"Ribosomal Protein S6 Kinases, 70-kDa","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D058570","descriptor_name":"TOR Serine-Threonine Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m413995200","doi":"https://doi.org/10.1074/jbc.m413995200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m413995200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:15659381","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15659381","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m413995200","doi":"https://doi.org/10.1074/jbc.m413995200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m413995200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6281187208","display_name":"Signal Transduction to P70 S6 Kinase 1","funder_award_id":"R01GM051405","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W1489003564","https://openalex.org/W1585385265","https://openalex.org/W1984736455","https://openalex.org/W1992480202","https://openalex.org/W2002031023","https://openalex.org/W2012142261","https://openalex.org/W2015674575","https://openalex.org/W2033238056","https://openalex.org/W2057875362","https://openalex.org/W2058012673","https://openalex.org/W2071618706","https://openalex.org/W2081659685","https://openalex.org/W2089731973","https://openalex.org/W2110443482","https://openalex.org/W2114106527","https://openalex.org/W2115479382","https://openalex.org/W2139856846","https://openalex.org/W2141560789","https://openalex.org/W2150446853","https://openalex.org/W2163583831","https://openalex.org/W7096186325"],"related_works":["https://openalex.org/W1873124068","https://openalex.org/W2002031023","https://openalex.org/W1983112008","https://openalex.org/W2057875362","https://openalex.org/W2277777756","https://openalex.org/W2012142261","https://openalex.org/W2052583546","https://openalex.org/W4322487486","https://openalex.org/W4238942796","https://openalex.org/W1987991478"],"abstract_inverted_index":{"The":[0],"mammalian":[1],"target":[2],"of":[3,37,63,92,107,202],"rapamycin,":[4],"mTOR,":[5],"is":[6,40,86,118,205],"a":[7,27,80,199],"Ser/Thr":[8],"kinase":[9,21,105],"that":[10,39,50,85,125,140,176,193,204],"promotes":[11],"cell":[12],"growth":[13],"and":[14,114,138,152,167],"proliferation":[15],"by":[16,186,208],"activating":[17],"ribosomal":[18],"protein":[19],"S6":[20],"1":[22],"(S6K1).":[23],"We":[24,77,191],"previously":[25],"identified":[26,79],"conserved":[28,81],"TOR":[29],"signaling":[30],"(TOS)":[31],"motif":[32,53,101,111,128,154,180,196],"in":[33],"the":[34,51,60,69,74,104,108,126,149,157,177,187,194],"N":[35],"terminus":[36,62],"S6K1":[38,93,109,131,144,160,203],"required":[41],"for":[42,88,143],"its":[43,165],"mTOR-dependent":[44,90],"activation.":[45,94,145],"Furthermore,":[46,121],"our":[47],"data":[48,174],"suggested":[49],"TOS":[52,110,179],"suppresses":[54,130,181],"an":[55,89,182],"inhibitory":[56,71,183],"function":[57,184],"associated":[58],"with":[59,198],"C":[61,75],"S6K1.":[64],"Here,":[65],"we":[66,122],"have":[67,78,123],"characterized":[68],"mTOR-regulated":[70],"region":[72],"within":[73,98],"terminus.":[76],"C-terminal":[82,188],"\"RSPRR\"":[83],"sequence":[84],"responsible":[87],"suppression":[91],"Deletion":[95],"or":[96],"mutations":[97,155],"this":[99,115],"RSPRR":[100,127,153,189,195],"partially":[102],"rescue":[103],"activity":[106,117,166],"mutant":[112,161],"(S6K1-F5A),":[113],"rescued":[116],"rapamycin":[119,171],"resistant.":[120,172],"shown":[124],"significantly":[129],"phosphorylation":[132,135],"at":[133],"two":[134],"sites":[136],"(Thr-389":[137],"Thr-229)":[139],"are":[141],"crucial":[142],"Importantly,":[146],"introducing":[147],"both":[148],"Thr-389":[150],"phosphomimetic":[151],"into":[156],"catalytically":[158],"inactive":[159],"S6K1-F5A":[162],"completely":[163],"rescues":[164],"renders":[168],"it":[169],"fully":[170],"These":[173],"show":[175],"N-terminal":[178],"mediated":[185],"motif.":[190],"propose":[192],"interacts":[197],"negative":[200],"regulator":[201],"normally":[206],"suppressed":[207],"mTOR.":[209]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}