{"id":"https://openalex.org/W2060829902","doi":"https://doi.org/10.1089/hyb.1987.6.605","title":"Screening for Anti-Idiotypic Monoclonal Antibodies on Paraformaldehyde-Fixed Lymphoma Cells","display_name":"Screening for Anti-Idiotypic Monoclonal Antibodies on Paraformaldehyde-Fixed Lymphoma Cells","publication_year":1987,"publication_date":"1987-12-01","ids":{"openalex":"https://openalex.org/W2060829902","doi":"https://doi.org/10.1089/hyb.1987.6.605","mag":"2060829902","pmid":"https://pubmed.ncbi.nlm.nih.gov/3325402"},"language":"en","primary_location":{"id":"doi:10.1089/hyb.1987.6.605","doi":"https://doi.org/10.1089/hyb.1987.6.605","is_oa":false,"landing_page_url":"https://doi.org/10.1089/hyb.1987.6.605","pdf_url":null,"source":{"id":"https://openalex.org/S4210200239","display_name":"Hybridoma","issn_l":"0272-457X","issn":["0272-457X","1554-0014","1557-8348","2168-7897"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hybridoma","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000004697","display_name":"Michael K. Samoszuk","orcid":"https://orcid.org/0000-0002-0588-8091","observed_orcids":["https://orcid.org/0000-0002-0588-8091"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"MICHAEL SAMOSZUK","raw_affiliation_strings":["Pathology Department, University of California, Irvine 92717"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pathology Department, University of California, Irvine 92717","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066863801","display_name":"Steven Sholly","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"STEVEN SHOLLY","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077143749","display_name":"Alan L. Epstein","orcid":"https://orcid.org/0000-0002-1834-7729","observed_orcids":["https://orcid.org/0000-0002-1834-7729"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"ALAN L. EPSTEIN","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.4143,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.82050461,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"6","issue":"6","first_page":"605","last_page":"610"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/anti-idiotypic-monoclonal-antibody","display_name":"anti-idiotypic monoclonal antibody","score":0.9739999771118164},{"id":"https://openalex.org/keywords/hybridoma-screening","display_name":"hybridoma screening","score":0.6539999842643738},{"id":"https://openalex.org/keywords/paraformaldehyde-fixation","display_name":"paraformaldehyde fixation","score":0.9760000109672546},{"id":"https://openalex.org/keywords/lymphoma-cells","display_name":"lymphoma cells","score":0.9179999828338623},{"id":"https://openalex.org/keywords/indirect-immunofluorescence","display_name":"indirect immunofluorescence","score":0.9259999990463257},{"id":"https://openalex.org/keywords/flow-cytometry-comparison","display_name":"flow cytometry comparison","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C2778723968","wikidata":"https://www.wikidata.org/wiki/Q418566","display_name":"Paraformaldehyde","level":2,"score":0.9592669010162354},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.9143847823143005},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.692416787147522},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5645477175712585},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5603849291801453},{"id":"https://openalex.org/C2779338263","wikidata":"https://www.wikidata.org/wiki/Q208414","display_name":"Lymphoma","level":2,"score":0.5498976707458496},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.5405927896499634},{"id":"https://openalex.org/C163864487","wikidata":"https://www.wikidata.org/wiki/Q6901461","display_name":"Monoclonal","level":4,"score":0.49950361251831055},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4525515139102936},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.34473657608032227},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3259889483451843},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3134692311286926},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.21563997864723206}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000888","descriptor_name":"Antibodies, Anti-Idiotypic","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000888","descriptor_name":"Antibodies, Anti-Idiotypic","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D005404","descriptor_name":"Fixatives","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005557","descriptor_name":"Formaldehyde","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006825","descriptor_name":"Hybridomas","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D007130","descriptor_name":"Immunoglobulin Idiotypes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D008223","descriptor_name":"Lymphoma","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1089/hyb.1987.6.605","doi":"https://doi.org/10.1089/hyb.1987.6.605","is_oa":false,"landing_page_url":"https://doi.org/10.1089/hyb.1987.6.605","pdf_url":null,"source":{"id":"https://openalex.org/S4210200239","display_name":"Hybridoma","issn_l":"0272-457X","issn":["0272-457X","1554-0014","1557-8348","2168-7897"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hybridoma","raw_type":"journal-article"},{"id":"pmid:3325402","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3325402","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hybridoma","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","score":0.5099999904632568,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4635214664","display_name":null,"funder_award_id":"1 KO8 CA01161-01","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G506575037","display_name":"NCI CLINICAL INVESTIGATOR AWARD PROGRAM","funder_award_id":"1k08ca001161-01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W166376016","https://openalex.org/W1513433026","https://openalex.org/W1550930711","https://openalex.org/W2070283605","https://openalex.org/W2243999061","https://openalex.org/W2472024822"],"related_works":["https://openalex.org/W2063274372","https://openalex.org/W2487111276","https://openalex.org/W2005960812","https://openalex.org/W2087739773","https://openalex.org/W2051692974","https://openalex.org/W2055613980","https://openalex.org/W1636020373","https://openalex.org/W1987454928","https://openalex.org/W2059973399","https://openalex.org/W1550930711"],"abstract_inverted_index":{"Paraformaldehyde-fixed,":[0],"human":[1],"lymphoma":[2],"cells":[3,32],"on":[4],"glass":[5],"slides":[6],"were":[7],"used":[8],"to":[9,28],"screen":[10],"murine":[11],"hybridoma":[12],"supernatants":[13],"for":[14,33,80],"anti-idiotypic":[15,82],"monoclonal":[16,83],"antibodies":[17],"by":[18,49,53],"a":[19],"rapid":[20],"indirect":[21],"immunofluorescence":[22],"technique.":[23],"The":[24,67],"method":[25],"is":[26],"shown":[27],"require":[29],"far":[30],"fewer":[31],"screening":[34,48],"than":[35],"current":[36,78],"techniques,":[37],"and":[38],"it":[39],"provides":[40],"results":[41,46],"that":[42,58],"correlate":[43],"with":[44],"the":[45,62],"of":[47,65],"flow":[50],"cytometry":[51],"or":[52],"capture":[54],"ELISA.":[55],"We":[56],"conclude":[57],"paraformaldehyde":[59],"fixation":[60],"preserves":[61],"idiotypic":[63],"determinants":[64],"lymphomas.":[66],"assay":[68],"described":[69],"in":[70],"this":[71],"report,":[72],"therefore,":[73],"has":[74],"significant":[75],"advantages":[76],"over":[77],"methods":[79],"producing":[81],"antibodies.":[84]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}