{"id":"https://openalex.org/W206038790","doi":null,"title":"Cytotoxicity of Thymoquinone (TQ) from Nigella sativa Towards Human Cervical Carcinoma Cells (HeLa)","display_name":"Cytotoxicity of Thymoquinone (TQ) from Nigella sativa Towards Human Cervical Carcinoma Cells (HeLa)","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W206038790","mag":"206038790"},"language":"en","primary_location":{"id":"pmh:oai:CiteSeerX.psu:10.1.1.736.5323","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.736.5323","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://jprsolutions.info/files/final-file-56947379e1acc5.59305475.pdf","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075531224","display_name":"Latifah Saiful Yazan","orcid":"https://orcid.org/0000-0001-7712-4990","observed_orcids":["https://orcid.org/0000-0001-7712-4990"]},"institutions":[{"id":"https://openalex.org/I130343225","display_name":"Universiti Putra Malaysia","ror":"https://ror.org/02e91jd64","country_code":"MY","type":"education","lineage":["https://openalex.org/I130343225"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Latifah Saiful Yazan","raw_affiliation_strings":["UNIVERSITI PUTRA MALAYSIA"],"raw_orcid":"https://orcid.org/0000-0001-7712-4990","affiliations":[{"raw_affiliation_string":"UNIVERSITI PUTRA MALAYSIA","institution_ids":["https://openalex.org/I130343225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109344050","display_name":"Wei Keat Ng","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wei Keat Ng","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068368608","display_name":"Ghanya Al\u2010Naqeb","orcid":"https://orcid.org/0000-0002-7734-1706","observed_orcids":["https://orcid.org/0000-0002-7734-1706"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ghanya Al\u2010Naqeb","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-7734-1706","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5019508937","display_name":"Maznah Ismail","orcid":"https://orcid.org/0000-0002-2378-0519","observed_orcids":["https://orcid.org/0000-0002-2378-0519"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Maznah Ismail","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-2378-0519","affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.7987,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.93170644,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"2","issue":"4","first_page":"585","last_page":"589"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12750","display_name":"Nigella sativa pharmacological applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12750","display_name":"Nigella sativa pharmacological applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11332","display_name":"Genomics, phytochemicals, and oxidative stress","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14521","display_name":"Medicinal Plant Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thymoquinone","display_name":"thymoquinone","score":1.0},{"id":"https://openalex.org/keywords/nigella-sativa","display_name":"Nigella sativa","score":1.0},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.7929999828338623},{"id":"https://openalex.org/keywords/hela-cells","display_name":"HeLa cells","score":0.847000002861023},{"id":"https://openalex.org/keywords/cervical-cancer","display_name":"cervical cancer","score":0.9810000061988831},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.7400000095367432},{"id":"https://openalex.org/keywords/cell-cycle-arrest","display_name":"cell cycle arrest","score":0.8840000033378601},{"id":"https://openalex.org/keywords/p53-expression","display_name":"p53 expression","score":0.8899999856948853},{"id":"https://openalex.org/keywords/antiproliferative-activity","display_name":"antiproliferative activity","score":0.9200000166893005},{"id":"https://openalex.org/keywords/ic50","display_name":"IC50","score":0.7080000042915344}],"concepts":[{"id":"https://openalex.org/C2779034068","wikidata":"https://www.wikidata.org/wiki/Q7799650","display_name":"Thymoquinone","level":3,"score":0.9207068085670471},{"id":"https://openalex.org/C2780070996","wikidata":"https://www.wikidata.org/wiki/Q61482373","display_name":"Trypan blue","level":3,"score":0.900375247001648},{"id":"https://openalex.org/C2777366897","wikidata":"https://www.wikidata.org/wiki/Q847482","display_name":"HeLa","level":3,"score":0.8649018406867981},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.7886191606521606},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.6108763217926025},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.6038809418678284},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5572614073753357},{"id":"https://openalex.org/C2780783641","wikidata":"https://www.wikidata.org/wiki/Q738686","display_name":"MTT assay","level":3,"score":0.5489910840988159},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5334907174110413},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.49256807565689087},{"id":"https://openalex.org/C2993714443","wikidata":"https://www.wikidata.org/wiki/Q160575","display_name":"Nigella sativa","level":2,"score":0.4412245750427246},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.40852147340774536},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3831978440284729},{"id":"https://openalex.org/C2778004101","wikidata":"https://www.wikidata.org/wiki/Q133948","display_name":"Antioxidant","level":2,"score":0.34291958808898926},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.32838281989097595},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28329747915267944},{"id":"https://openalex.org/C556039675","wikidata":"https://www.wikidata.org/wiki/Q771035","display_name":"Traditional medicine","level":1,"score":0.2656400501728058},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2450307011604309},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2310371696949005},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:CiteSeerX.psu:10.1.1.736.5323","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.736.5323","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://jprsolutions.info/files/final-file-56947379e1acc5.59305475.pdf","raw_type":"text"},{"id":"pmh:oai:psasir.upm.edu.my:13864","doi":null,"is_oa":false,"landing_page_url":"http://psasir.upm.edu.my/id/eprint/13864/","pdf_url":null,"source":{"id":"https://openalex.org/S4377196260","display_name":"Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I130343225","host_organization_name":"Universiti Putra Malaysia","host_organization_lineage":["https://openalex.org/I130343225"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"mag:206038790","doi":null,"is_oa":false,"landing_page_url":"http://psasir.upm.edu.my/id/eprint/13864/","pdf_url":null,"source":{"id":"https://openalex.org/S2765036248","display_name":"Journal of Pharmacy Research","issn_l":"0974-6943","issn":["0974-6943"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Journal of Pharmacy Research","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.98580002784729,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1520044475","https://openalex.org/W1991455005","https://openalex.org/W1994445467","https://openalex.org/W2016505914","https://openalex.org/W2021399542","https://openalex.org/W2029080338","https://openalex.org/W2051899483","https://openalex.org/W2053104672","https://openalex.org/W2061018452","https://openalex.org/W2062261425","https://openalex.org/W2069581132","https://openalex.org/W2069755793","https://openalex.org/W2079809624","https://openalex.org/W2086369936","https://openalex.org/W2088523359","https://openalex.org/W2101589435","https://openalex.org/W2114918609","https://openalex.org/W2129605627","https://openalex.org/W2171407623"],"related_works":["https://openalex.org/W4322748335","https://openalex.org/W2020963110","https://openalex.org/W3137769850","https://openalex.org/W206841795","https://openalex.org/W2106712418","https://openalex.org/W2913097514","https://openalex.org/W2090382739","https://openalex.org/W2185099364","https://openalex.org/W2742705575","https://openalex.org/W2567777839"],"abstract_inverted_index":{"Received":[0],"on:12-12-2008;":[1],"Accepted":[2],"on:07-02-2009":[3],"Thymoquinone":[4],"(TQ),":[5],"the":[6,10,35,57,87,95,117,187,195,216,219,224],"bioactive":[7],"constituent":[8],"of":[9,13,37,62,89,164,184,190,206,215,218],"volatile":[11],"oil":[12],"Nigella":[14],"sativa,":[15],"has":[16],"been":[17],"shown":[18],"to":[19,130,223],"exert":[20],"anti-inflammatory,":[21],"anti-oxidant":[22],"and":[23,29,53,64,80,101,161,173],"anti-neoplastic":[24],"effects":[25],"both":[26],"in":[27,30,86,109,139,155,186,212],"vitro":[28],"vivo.":[31],"In":[32],"this":[33],"study,":[34],"cytotoxicity":[36,108,138],"TQ":[38,50,106,136,147,178,207],"was":[39,92],"evaluated":[40],"on":[41],"human":[42],"cervical":[43],"carcinoma":[44],"cells":[45,58,144,202],"(HeLa).":[46],"Results":[47],"showed":[48,151,199],"that":[49,105,135,177,200],"exhibited":[51],"cytotoxic":[52],"anti-proliferative":[54],"activities":[55],"towards":[56],"with":[59,97,146,204],"IC50":[60,113],"value":[61],"2.80\u00b10.10mg/ml":[63,131],"5.37\u00b10.12mg/ml":[65],"after":[66,94],"72":[67,149,209],"hours":[68,150,210],"incubation":[69],"time":[70],"as":[71],"being":[72],"detected":[73,192],"by":[74,116,182,193],"trypan":[75,118],"blue":[76,119],"dye":[77,120],"exclusion":[78,121],"test":[79,122],"MTT":[81],"assay,":[82],"respectively.":[83],"Significant":[84],"decrease":[85,154],"percentage":[88],"cell":[90,156,165,180],"viability":[91],"observed":[93],"treatment":[96],"1.0,":[98],"3.0,":[99],"10":[100,172],"30mg/ml":[102,174],"(p<0.05)":[103,221],"indicating":[104],"induced":[107,137],"a":[110,140,152],"dose-dependent":[111],"manner.":[112,142],"values":[114],"determined":[115],"were":[123],"significantly":[124],"decreased":[125],"from":[126],"5.93\u00b10.81mg/ml":[127],"(24":[128],"hours)":[129,133],"(72":[132],"suggesting":[134,176],"time-dependent":[141],"HeLa":[143,201],"treated":[145],"for":[148,208],"significant":[153,162,213],"population":[157,166],"at":[158,167,170],"G0/G1":[159],"phase":[160,169],"increase":[163],"sub-G1":[168],"6.0,":[171],"(p<0.05),":[175],"inhibited":[179],"proliferation":[181],"induction":[183],"apoptosis":[185],"cells.":[188],"Expression":[189],"p53":[191,197],"using":[194],"Human":[196],"ELISA":[198],"incubated":[203],"10mg/ml":[205],"resulted":[211],"up-regulation":[214],"expression":[217],"protein":[220],"compared":[222],"control":[225],"untreated":[226],"sample.":[227],"It":[228],"is":[229]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}