{"id":"https://openalex.org/W2060071007","doi":"https://doi.org/10.1126/science.3175665","title":"Heat Shock Is Lethal to Fibroblasts Microinjected with Antibodies Against hsp70","display_name":"Heat Shock Is Lethal to Fibroblasts Microinjected with Antibodies Against hsp70","publication_year":1988,"publication_date":"1988-10-21","ids":{"openalex":"https://openalex.org/W2060071007","doi":"https://doi.org/10.1126/science.3175665","mag":"2060071007","pmid":"https://pubmed.ncbi.nlm.nih.gov/3175665"},"language":"en","primary_location":{"id":"doi:10.1126/science.3175665","doi":"https://doi.org/10.1126/science.3175665","is_oa":false,"landing_page_url":"https://doi.org/10.1126/science.3175665","pdf_url":null,"source":{"id":"https://openalex.org/S3880285","display_name":"Science","issn_l":"0036-8075","issn":["0036-8075","0193-4511","1095-9203"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315823","host_organization_name":"American Association for the Advancement of Science","host_organization_lineage":["https://openalex.org/P4310315823"],"host_organization_lineage_names":["American Association for the Advancement of Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2060071007"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081300202","display_name":"Karl T. Riabowol","orcid":"https://orcid.org/0000-0002-5318-2536","observed_orcids":["https://orcid.org/0000-0002-5318-2536"]},"institutions":[{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karl T. Riabowol","raw_affiliation_strings":["Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724","institution_ids":["https://openalex.org/I2750212522"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078358608","display_name":"Lee Mizzen","orcid":"https://orcid.org/0000-0001-6723-8441","observed_orcids":["https://orcid.org/0000-0001-6723-8441"]},"institutions":[{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lee A. Mizzen","raw_affiliation_strings":["Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724","institution_ids":["https://openalex.org/I2750212522"]}]},{"author_position":"last","author":{"id":null,"display_name":"William J. Welch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William J. Welch","raw_affiliation_strings":["Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724","institution_ids":["https://openalex.org/I2750212522"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I2750212522"],"apc_list":null,"apc_paid":null,"fwci":12.3752,"has_fulltext":false,"cited_by_count":626,"citation_normalized_percentile":{"value":0.99282887,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"242","issue":"4877","first_page":"433","last_page":"436"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11150","display_name":"Endoplasmic Reticulum Stress and Disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.8050000071525574},{"id":"https://openalex.org/keywords/hsp70","display_name":"HSP70","score":0.9990000128746033},{"id":"https://openalex.org/keywords/heat-shock","display_name":"heat shock","score":0.7459999918937683},{"id":"https://openalex.org/keywords/cell-survival","display_name":"cell survival","score":0.5559999942779541},{"id":"https://openalex.org/keywords/thermal-stress-biology","display_name":"thermal stress (biology)","score":0.8669999837875366},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8740000128746033},{"id":"https://openalex.org/keywords/antibody-microinjection","display_name":"antibody microinjection","score":0.8119999766349792},{"id":"https://openalex.org/keywords/fibroblasts","display_name":"fibroblasts","score":0.8980000019073486},{"id":"https://openalex.org/keywords/nuclear-translocation","display_name":"nuclear translocation","score":0.8999999761581421},{"id":"https://openalex.org/keywords/stress-response","display_name":"stress response","score":0.6079999804496765}],"concepts":[{"id":"https://openalex.org/C68991219","wikidata":"https://www.wikidata.org/wiki/Q419102","display_name":"Hsp70","level":4,"score":0.8224445581436157},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.7714830636978149},{"id":"https://openalex.org/C62525196","wikidata":"https://www.wikidata.org/wiki/Q3311945","display_name":"Microinjection","level":2,"score":0.7112593650817871},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.587276816368103},{"id":"https://openalex.org/C2781300812","wikidata":"https://www.wikidata.org/wiki/Q178061","display_name":"Shock (circulatory)","level":2,"score":0.5678579807281494},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.5589348077774048},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5173965692520142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49750521779060364},{"id":"https://openalex.org/C2780723820","wikidata":"https://www.wikidata.org/wiki/Q1934178","display_name":"Nucleus","level":2,"score":0.4786897301673889},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4552610516548157},{"id":"https://openalex.org/C137984847","wikidata":"https://www.wikidata.org/wiki/Q1621258","display_name":"Heat shock","level":4,"score":0.45090144872665405},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3249872922897339},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23057174682617188},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.19449195265769958},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.13263177871704102},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.06415724754333496},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":false},{"descriptor_ui":"D000936","descriptor_name":"Antigen-Antibody Complex","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006360","descriptor_name":"Heat-Shock Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006360","descriptor_name":"Heat-Shock Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1126/science.3175665","doi":"https://doi.org/10.1126/science.3175665","is_oa":false,"landing_page_url":"https://doi.org/10.1126/science.3175665","pdf_url":null,"source":{"id":"https://openalex.org/S3880285","display_name":"Science","issn_l":"0036-8075","issn":["0036-8075","0193-4511","1095-9203"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315823","host_organization_name":"American Association for the Advancement of Science","host_organization_lineage":["https://openalex.org/P4310315823"],"host_organization_lineage_names":["American Association for the Advancement of Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science","raw_type":"journal-article"},{"id":"pmid:3175665","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3175665","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science (New York, N.Y.)","raw_type":"Journal Article"},{"id":"pmh:oai:repository.cshl.edu:35206","doi":null,"is_oa":false,"landing_page_url":"https://repository.cshl.edu/id/eprint/35206/","pdf_url":null,"source":{"id":"https://openalex.org/S4306402288","display_name":"Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Paper"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.46810001134872437,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3198669182","display_name":"Structure Function of the Mammalian Stress Proteins","funder_award_id":"R01GM033551","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1614185919","https://openalex.org/W1618691523","https://openalex.org/W1888409122","https://openalex.org/W1961919679","https://openalex.org/W1964892712","https://openalex.org/W1966907932","https://openalex.org/W1968796017","https://openalex.org/W1972458635","https://openalex.org/W1977388184","https://openalex.org/W1981718777","https://openalex.org/W2005383788","https://openalex.org/W2012333825","https://openalex.org/W2013024855","https://openalex.org/W2031684542","https://openalex.org/W2051180448","https://openalex.org/W2058333572","https://openalex.org/W2067128865","https://openalex.org/W2082205973","https://openalex.org/W2088603473","https://openalex.org/W2093141883","https://openalex.org/W2107774451","https://openalex.org/W2130249954","https://openalex.org/W2165113979","https://openalex.org/W2229542208","https://openalex.org/W2292476398"],"related_works":["https://openalex.org/W2003088600","https://openalex.org/W7217246593","https://openalex.org/W785411030","https://openalex.org/W7098482371","https://openalex.org/W2013814855","https://openalex.org/W1966907932","https://openalex.org/W2087675686","https://openalex.org/W4300110223","https://openalex.org/W1976963083","https://openalex.org/W1614124891"],"abstract_inverted_index":{"Synthesis":[0],"of":[1,5,24,61,93,137],"a":[2,21,37,110,123],"small":[3],"group":[4],"highly":[6,64],"conserved":[7,65],"proteins":[8,35,95],"in":[9,39,54],"response":[10],"to":[11,51,77,88,108],"elevated":[12],"temperature":[13],"and":[14,26,43,141],"other":[15],"agents":[16],"that":[17,33,130],"induce":[18],"stress":[19],"is":[20,47,133],"universal":[22],"feature":[23],"prokaryotic":[25],"eukaryotic":[27],"cells.":[28,56],"Although":[29],"correlative":[30],"evidence":[31,50],"suggests":[32],"these":[34,138],"play":[36],"role":[38,60],"enhancing":[40],"survival":[41,136],"during":[42,71,140],"after":[44,99,142],"stress,":[45],"there":[46],"no":[48],"direct":[49],"support":[52],"this":[53],"mammalian":[55],"To":[57],"assess":[58],"the":[59,62,90,97],"most":[63],"heat":[66,72,101,125],"shock":[67,102],"protein":[68],"(hsp)":[69],"family":[70],"shock,":[73],"affinity-purified":[74],"monoclonal":[75],"antibodies":[76,121],"hsp70":[78,94,132],"were":[79,106],"introduced":[80],"into":[81,96],"fibroblasts":[82],"by":[83],"needle":[84],"microinjection.":[85],"In":[86],"addition":[87],"impairing":[89],"heat-induced":[91],"translocation":[92],"nucleus":[98],"mild":[100],"treatment,":[103],"injected":[104,118],"cells":[105,139],"unable":[107],"survive":[109],"brief":[111],"incubation":[112],"at":[113],"45":[114],"degrees":[115],"C.":[116],"Cells":[117],"with":[119],"control":[120],"survived":[122],"similar":[124],"shock.":[126],"These":[127],"results":[128],"indicate":[129],"functional":[131],"required":[134],"for":[135],"thermal":[143],"stress.":[144]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}