{"id":"https://openalex.org/W2059976588","doi":"https://doi.org/10.1074/jbc.m111.254219","title":"Shortened Engineered Human Antibody CH2 Domains","display_name":"Shortened Engineered Human Antibody CH2 Domains","publication_year":2011,"publication_date":"2011-06-14","ids":{"openalex":"https://openalex.org/W2059976588","doi":"https://doi.org/10.1074/jbc.m111.254219","mag":"2059976588","pmid":"https://pubmed.ncbi.nlm.nih.gov/21669873","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3149322"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m111.254219","doi":"https://doi.org/10.1074/jbc.m111.254219","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m111.254219","pdf_url":"http://www.jbc.org/article/S0021925820503347/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925820503347/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111782319","display_name":"Rui Gong","orcid":"https://orcid.org/0000-0001-9222-5642","observed_orcids":["https://orcid.org/0000-0001-9222-5642"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rui Gong","raw_affiliation_strings":["Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702, USA","Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"]},{"raw_affiliation_string":"Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100605203","display_name":"Yanping Wang","orcid":"https://orcid.org/0000-0002-5842-715X","observed_orcids":["https://orcid.org/0000-0002-5842-715X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yanping Wang","raw_affiliation_strings":["Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702","SAIC-Frederick, Inc., NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"]},{"raw_affiliation_string":"SAIC-Frederick, Inc., NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112690536","display_name":"Yang Feng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yang Feng","raw_affiliation_strings":["Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028355034","display_name":"Qi Zhao","orcid":"https://orcid.org/0000-0002-5969-6407","observed_orcids":["https://orcid.org/0000-0002-5969-6407"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Qi Zhao","raw_affiliation_strings":["Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089862290","display_name":"Dimiter Stanchev Dimitrov","orcid":"https://orcid.org/0000-0002-2258-1024","observed_orcids":["https://orcid.org/0000-0002-2258-1024"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Dimiter S. Dimitrov","raw_affiliation_strings":["Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Protein Interactions Group, Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Institutes of Health, Frederick, Maryland 21702","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5089862290"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210149717"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":5.8666,"has_fulltext":true,"cited_by_count":52,"citation_normalized_percentile":{"value":0.96302928,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"286","issue":"31","first_page":"27288","last_page":"27293"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.010900000110268593,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.699999988079071},{"id":"https://openalex.org/keywords/thermal-denaturation","display_name":"thermal denaturation","score":0.734000027179718},{"id":"https://openalex.org/keywords/disulfide-bond-engineering","display_name":"disulfide bond engineering","score":0.8989999890327454},{"id":"https://openalex.org/keywords/n-terminal-truncation","display_name":"N-terminal truncation","score":0.8489999771118164},{"id":"https://openalex.org/keywords/conformational-epitopes","display_name":"conformational epitopes","score":0.847000002861023},{"id":"https://openalex.org/keywords/fcrn","display_name":"neonatal Fc receptor","score":0.8669999837875366},{"id":"https://openalex.org/keywords/antibody-effector-functions","display_name":"antibody effector functions","score":0.7570000290870667},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.4970000088214874},{"id":"https://openalex.org/keywords/protease-resistance","display_name":"protease resistance","score":0.890999972820282}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7265015840530396},{"id":"https://openalex.org/C34349673","wikidata":"https://www.wikidata.org/wiki/Q899124","display_name":"Random coil","level":3,"score":0.5926527976989746},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.586520791053772},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.5616611838340759},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5386514663696289},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.46746304631233215},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.4442134499549866},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3975071310997009},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39012476801872253},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3217664957046509},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.32085686922073364},{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.2825695872306824},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.16002413630485535},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14032170176506042},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.09233155846595764},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007231","descriptor_name":"Infant, Newborn","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011961","descriptor_name":"Receptors, Fc","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019906","descriptor_name":"Nuclear Magnetic Resonance, Biomolecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1074/jbc.m111.254219","doi":"https://doi.org/10.1074/jbc.m111.254219","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m111.254219","pdf_url":"http://www.jbc.org/article/S0021925820503347/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2417282","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3149322","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3149322","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3149322","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biol Chem","raw_type":"Text"},{"id":"pmid:21669873","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21669873","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m111.254219","doi":"https://doi.org/10.1074/jbc.m111.254219","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m111.254219","pdf_url":"http://www.jbc.org/article/S0021925820503347/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1356092923","display_name":null,"funder_award_id":"N01CO12400","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G174812244","display_name":"DCTD Computer Support","funder_award_id":"n01co12400-79-0-22","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G1799052500","display_name":null,"funder_award_id":"N01-CO-012400","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G3277247684","display_name":null,"funder_award_id":"N01-CO-12400","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2059976588.pdf","grobid_xml":"https://content.openalex.org/works/W2059976588.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W199166898","https://openalex.org/W1532082174","https://openalex.org/W1586605199","https://openalex.org/W1969305630","https://openalex.org/W1987654889","https://openalex.org/W2005003298","https://openalex.org/W2016690055","https://openalex.org/W2018295245","https://openalex.org/W2021256658","https://openalex.org/W2038911138","https://openalex.org/W2041177153","https://openalex.org/W2043532306","https://openalex.org/W2052505196","https://openalex.org/W2058915223","https://openalex.org/W2059594199","https://openalex.org/W2065620086","https://openalex.org/W2065654184","https://openalex.org/W2108796583","https://openalex.org/W2117131474","https://openalex.org/W2138906847","https://openalex.org/W4313311282"],"related_works":["https://openalex.org/W2117131474","https://openalex.org/W2993846589","https://openalex.org/W2961576737","https://openalex.org/W2344344719","https://openalex.org/W1968243973","https://openalex.org/W2957804804","https://openalex.org/W4388339561","https://openalex.org/W2052505196","https://openalex.org/W7097484905","https://openalex.org/W2890364443"],"abstract_inverted_index":{"The":[0,146],"immunoglobulin":[1],"(Ig)":[2],"constant":[3],"CH2":[4,13,40,140,150,181,217],"domain":[5,41,107],"is":[6,42,168],"critical":[7],"for":[8,18,261],"antibody":[9,106,201],"effector":[10,30],"functions.":[11,31],"Isolated":[12],"domains":[14],"are":[15,127],"promising":[16],"scaffolds":[17],"construction":[19],"of":[20,103,137,164,177],"libraries":[21],"containing":[22],"diverse":[23],"binders":[24],"that":[25,36,100,126,202,244,258],"could":[26,108],"also":[27,272],"confer":[28],"some":[29],"We":[32,97],"have":[33,98],"shown":[34],"previously":[35],"an":[37,57],"isolated":[38,139],"human":[39,230],"relatively":[43],"unstable":[44],"to":[45,192,208,216,222,226,274],"thermally":[46],"induced":[47],"unfolding,":[48],"but":[49],"its":[50,255],"stability":[51,102,252,263],"can":[52],"be":[53,109],"improved":[54],"by":[55,112,134,267],"engineering":[56],"additional":[58],"disulfide":[59],"bond":[60],"(Gong,":[61],"R.,":[62,86],"Vu,":[63],"B.":[64],"K.,":[65],"Feng,":[66],"Y.,":[67],"Prieto,":[68],"D.":[69,89],"A.,":[70,73],"Dyba,":[71],"M.":[72],"Walsh,":[74],"J.":[75,92],"D.,":[76,81],"Prabakaran,":[77],"P.,":[78],"Veenstra,":[79],"T.":[80],"Tarasov,":[82],"S.":[83,90],"G.,":[84],"Ishima,":[85],"and":[87,143,156,171,183,187,218,257,264],"Dimitrov,":[88],"(2009)":[91],"Biol.":[93],"Chem.":[94],"284,":[95],"14203-14210).":[96],"hypothesized":[99],"the":[101,122,138,178,246],"this":[104],"engineered":[105,149],"further":[110],"increased":[111],"removing":[113,268],"unstructured":[114,269],"residues.":[115],"To":[116],"test":[117],"our":[118,135,259],"hypothesis,":[119],"we":[120],"removed":[121],"seven":[123],"N-terminal":[124],"residues":[125],"in":[128],"a":[129,160,204,227],"random":[130],"coil":[131],"as":[132],"suggested":[133],"analysis":[136],"crystal":[141],"structure":[142],"NMR":[144],"data.":[145],"resulting":[147],"shortened":[148],"(m01s)":[151],"was":[152],"highly":[153],"soluble,":[154],"monomeric,":[155],"remarkably":[157],"stable,":[158],"with":[159],"melting":[161],"temperature":[162],"(T(m))":[163],"82.6":[165],"\u00b0C,":[166],"which":[167],"about":[169],"10":[170],"30":[172],"\u00b0C":[173],"higher":[174,213],"than":[175,195,215,221,239],"those":[176],"original":[179],"stabilized":[180],"(m01)":[182],"CH2,":[184],"respectively.":[185],"m01s":[186,209,224],"m01":[188,247],"were":[189],"more":[190,237],"resistant":[191],"protease":[193],"digestion":[194],"CH2.":[196,240],"A":[197],"newly":[198],"identified":[199],"anti-CH2":[200],"recognizes":[203],"conformational":[205],"epitope":[206],"bound":[207,225],"significantly":[210,250],"better":[211,220],"(>10-fold":[212],"affinity)":[214],"slightly":[219],"m01.":[223],"recombinant":[228],"soluble":[229],"neonatal":[231],"Fc":[232],"receptor":[233],"at":[234],"pH":[235],"6.0":[236],"strongly":[238],"These":[241],"data":[242],"suggest":[243],"shortening":[245],"N":[248],"terminus":[249],"increases":[251],"without":[253],"disrupting":[254],"conformation":[256],"approach":[260],"increasing":[262],"decreasing":[265],"size":[266],"regions":[270],"may":[271],"apply":[273],"other":[275],"proteins.":[276]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":9}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}