{"id":"https://openalex.org/W2057318421","doi":"https://doi.org/10.1093/nar/gkn531","title":"An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase\u2013DNA complexes to surfaces","display_name":"An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase\u2013DNA complexes to surfaces","publication_year":2008,"publication_date":"2008-08-22","ids":{"openalex":"https://openalex.org/W2057318421","doi":"https://doi.org/10.1093/nar/gkn531","mag":"2057318421","pmid":"https://pubmed.ncbi.nlm.nih.gov/18723573","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2566871"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkn531","doi":"https://doi.org/10.1093/nar/gkn531","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkn531","pdf_url":"https://academic.oup.com/nar/article-pdf/36/18/e121/18780852/gkn531.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/36/18/e121/18780852/gkn531.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109238934","display_name":"John Garnett Williams","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John G. K. Williams","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA. john.williams@licor.com","Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA. john.williams@licor.com","institution_ids":["https://openalex.org/I149218723"]},{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075455365","display_name":"David L. Steffens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David L. Steffens","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103829705","display_name":"Jon P. Anderson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jon P. Anderson","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030787211","display_name":"Teresa M. Urlacher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Teresa M. Urlacher","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079548817","display_name":"Don T. Lamb","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald T. Lamb","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008207685","display_name":"Daniel L. Grone","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel L. Grone","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5068736102","display_name":"Jolene C. Egelhoff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149218723","display_name":"LI-COR Biosciences (United States)","ror":"https://ror.org/017shr829","country_code":"US","type":"company","lineage":["https://openalex.org/I149218723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jolene C. Egelhoff","raw_affiliation_strings":["Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Research & Development, LI-COR Biosciences, Lincoln, NE, USA","institution_ids":["https://openalex.org/I149218723"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5109238934"],"corresponding_institution_ids":["https://openalex.org/I149218723"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":{"value":3992,"currency":"USD","value_usd":3992},"fwci":0.68,"has_fulltext":true,"cited_by_count":13,"citation_normalized_percentile":{"value":0.67315175,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"36","issue":"18","first_page":"e121","last_page":"e121"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.4399000108242035,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.4399000108242035,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.1151999980211258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.09799999743700027,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-polymerase","display_name":"DNA polymerase","score":0.6309999823570251},{"id":"https://openalex.org/keywords/streptavidin","display_name":"streptavidin","score":0.8299999833106995},{"id":"https://openalex.org/keywords/oriented-immobilization","display_name":"oriented immobilization","score":0.7670000195503235},{"id":"https://openalex.org/keywords/archaeal-dna-polymerase","display_name":"archaeal DNA polymerase","score":0.7789999842643738},{"id":"https://openalex.org/keywords/single-molecule-dna-sequencing","display_name":"single-molecule DNA sequencing","score":0.7710000276565552},{"id":"https://openalex.org/keywords/polymerase-dna-complex","display_name":"polymerase-DNA complex","score":0.777999997138977},{"id":"https://openalex.org/keywords/surface-immobilization","display_name":"surface immobilization","score":0.5879999995231628},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.5090000033378601}],"concepts":[{"id":"https://openalex.org/C131140672","wikidata":"https://www.wikidata.org/wiki/Q2031164","display_name":"Processivity","level":4,"score":0.9345630407333374},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8699383735656738},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.6631525158882141},{"id":"https://openalex.org/C31254050","wikidata":"https://www.wikidata.org/wiki/Q22328799","display_name":"DNA clamp","level":5,"score":0.65578693151474},{"id":"https://openalex.org/C2776190903","wikidata":"https://www.wikidata.org/wiki/Q421817","display_name":"Streptavidin","level":3,"score":0.6283690929412842},{"id":"https://openalex.org/C2776161997","wikidata":"https://www.wikidata.org/wiki/Q846600","display_name":"Clamp","level":3,"score":0.5888941884040833},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5835651755332947},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.5701387524604797},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.559096097946167},{"id":"https://openalex.org/C164965036","wikidata":"https://www.wikidata.org/wiki/Q2529148","display_name":"DNA polymerase I","level":5,"score":0.4210905134677887},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.35412150621414185},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.32932770252227783},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32700076699256897},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.32515382766723633},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3091481626033783},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.12925463914871216},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1104937195777893},{"id":"https://openalex.org/C2778204606","wikidata":"https://www.wikidata.org/wiki/Q181354","display_name":"Biotin","level":2,"score":0.09761714935302734},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.07698699831962585},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.0},{"id":"https://openalex.org/C84111939","wikidata":"https://www.wikidata.org/wiki/Q5125465","display_name":"Clamping","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004259","descriptor_name":"DNA-Directed DNA Polymerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004259","descriptor_name":"DNA-Directed DNA Polymerase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005615","descriptor_name":"Freezing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013698","descriptor_name":"Templates, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019809","descriptor_name":"Streptavidin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019921","descriptor_name":"Biotinylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/nar/gkn531","doi":"https://doi.org/10.1093/nar/gkn531","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkn531","pdf_url":"https://academic.oup.com/nar/article-pdf/36/18/e121/18780852/gkn531.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2566871","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2566871","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:18723573","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18723573","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1093/nar/gkn531","doi":"https://doi.org/10.1093/nar/gkn531","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkn531","pdf_url":"https://academic.oup.com/nar/article-pdf/36/18/e121/18780852/gkn531.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1604999283","display_name":null,"funder_award_id":"R44 HG002292","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G2598569130","display_name":null,"funder_award_id":"3R44HG002292-04S1","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G5805986","display_name":"DNA polymerase for single molecule DNA sequencing","funder_award_id":"5r44hg002292-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G7996048276","display_name":"Single-Molecule DNA Sequencing Using Charge-Switch dNTPs","funder_award_id":"P01HG003015","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2057318421.pdf","grobid_xml":"https://content.openalex.org/works/W2057318421.grobid-xml"},"referenced_works_count":33,"referenced_works":["https://openalex.org/W1486075102","https://openalex.org/W1491798811","https://openalex.org/W1493170168","https://openalex.org/W1592166638","https://openalex.org/W1981370593","https://openalex.org/W1982538875","https://openalex.org/W1985160176","https://openalex.org/W1988975446","https://openalex.org/W1990968510","https://openalex.org/W1996851263","https://openalex.org/W2014976263","https://openalex.org/W2020805838","https://openalex.org/W2026583935","https://openalex.org/W2032704055","https://openalex.org/W2035469525","https://openalex.org/W2049093445","https://openalex.org/W2050471630","https://openalex.org/W2055593712","https://openalex.org/W2056601391","https://openalex.org/W2070985991","https://openalex.org/W2076946539","https://openalex.org/W2104247890","https://openalex.org/W2106477570","https://openalex.org/W2111202021","https://openalex.org/W2114345540","https://openalex.org/W2122308238","https://openalex.org/W2125021618","https://openalex.org/W2140897574","https://openalex.org/W2154526671","https://openalex.org/W2155596249","https://openalex.org/W2158510501","https://openalex.org/W2162010421","https://openalex.org/W4234093616"],"related_works":["https://openalex.org/W2027956895","https://openalex.org/W2169224146","https://openalex.org/W2015375243","https://openalex.org/W2066776671","https://openalex.org/W59045488","https://openalex.org/W2419103704","https://openalex.org/W2034110126","https://openalex.org/W27976823","https://openalex.org/W7095260326","https://openalex.org/W2322238553"],"abstract_inverted_index":{"Single":[0],"molecule":[1,27,192],"analysis":[2],"of":[3,10,20,35,52,67,94,183],"individual":[4],"enzymes":[5],"can":[6,172],"require":[7],"oriented":[8],"immobilization":[9],"the":[11,32,64,68,86,95,99,103,107,137,145,159,167],"subject":[12],"molecules":[13],"on":[14,57,91,109,127],"a":[15,21,38,58,110,128],"detection":[16],"surface.":[17,60,112,129],"As":[18],"part":[19],"technology":[22],"development":[23],"project":[24],"for":[25,188],"single":[26,191],"DNA":[28,39,43,55,70,100,150],"sequencing,":[29],"we":[30,62],"faced":[31],"multiple":[33],"challenges":[34],"immobilizing":[36],"both":[37,97,122],"polymerase":[40,71,104,139],"and":[41,105,125,176],"its":[42],"template":[44,101],"together":[45],"in":[46,75,102,123,136,144,190],"an":[47],"active,":[48],"stable":[49],"complex":[50,108],"capable":[51],"highly":[53],"processive":[54],"synthesis":[56],"nonstick":[59],"Here,":[61],"report":[63],"genetic":[65],"modification":[66],"archaeal":[69],"9":[72],"degrees":[73],"N":[74],"which":[76],"two":[77],"biotinylated":[78,111],"peptide":[79],"'legs'":[80],"are":[81,120],"inserted":[82],"at":[83],"positions":[84],"flanking":[85],"DNA-binding":[87],"cleft.":[88],"Streptavidin":[89],"binding":[90],"either":[92],"side":[93],"cleft":[96],"traps":[98],"orients":[106],"We":[113],"present":[114],"evidence":[115],"that":[116],"purified":[117],"polymerase-DNA-streptavidin":[118,170],"complexes":[119,154,171],"active":[121],"solution":[124],"immobilized":[126,153],"Processivity":[130],"is":[131,155],"improved":[132],"from":[133],"<20":[134],"nt":[135],"unmodified":[138],"to":[140,166],"several":[141],"thousand":[142],"nucleotides":[143],"engineered":[146],"complexes.":[147],"High-molecular":[148],"weight":[149],"synthesized":[151],"by":[152],"observed":[156],"moving":[157],"above":[158],"surface":[160],"even":[161],"as":[162],"it":[163],"remains":[164],"tethered":[165],"polymerase.":[168],"Pre-formed":[169],"be":[173],"stored":[174],"frozen":[175],"subsequently":[177],"thawed":[178],"without":[179],"dissociation":[180],"or":[181],"loss":[182],"activity,":[184],"making":[185],"them":[186],"convenient":[187],"use":[189],"analysis.":[193]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}