{"id":"https://openalex.org/W2057316637","doi":"https://doi.org/10.1039/c4cp00208c","title":"Optical manipulation of single molecules in the living cell","display_name":"Optical manipulation of single molecules in the living cell","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2057316637","doi":"https://doi.org/10.1039/c4cp00208c","mag":"2057316637","pmid":"https://pubmed.ncbi.nlm.nih.gov/24651890"},"language":"en","primary_location":{"id":"doi:10.1039/c4cp00208c","doi":"https://doi.org/10.1039/c4cp00208c","is_oa":false,"landing_page_url":"https://doi.org/10.1039/c4cp00208c","pdf_url":null,"source":{"id":"https://openalex.org/S181332294","display_name":"Physical Chemistry Chemical Physics","issn_l":"1463-9076","issn":["1463-9076","1463-9084"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-3","ki-jl-1","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Phys. Chem. Chem. Phys.","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2057316637"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085508945","display_name":"Kamilla N\u00f8rregaard","orcid":"https://orcid.org/0000-0003-1953-9703","observed_orcids":["https://orcid.org/0000-0003-1953-9703"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Kamilla Norregaard","raw_affiliation_strings":["Copenhagen, Denmark","Niels Bohr Institute","University of Copenhagen","[Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark]"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Copenhagen, Denmark","institution_ids":[]},{"raw_affiliation_string":"Niels Bohr Institute","institution_ids":[]},{"raw_affiliation_string":"University of Copenhagen","institution_ids":["https://openalex.org/I124055696"]},{"raw_affiliation_string":"[Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark]","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031057899","display_name":"Liselotte Jauffred","orcid":"https://orcid.org/0000-0002-2223-9464","observed_orcids":["https://orcid.org/0000-0002-2223-9464"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Liselotte Jauffred","raw_affiliation_strings":["Copenhagen, Denmark","Niels Bohr Institute","University of Copenhagen","[Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark]"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Copenhagen, Denmark","institution_ids":[]},{"raw_affiliation_string":"Niels Bohr Institute","institution_ids":[]},{"raw_affiliation_string":"University of Copenhagen","institution_ids":["https://openalex.org/I124055696"]},{"raw_affiliation_string":"[Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark]","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001898424","display_name":"Kirstine Berg\u2010S\u00f8rensen","orcid":"https://orcid.org/0000-0002-9977-3980","observed_orcids":["https://orcid.org/0000-0002-9977-3980"]},"institutions":[{"id":"https://openalex.org/I96673099","display_name":"Technical University of Denmark","ror":"https://ror.org/04qtj9h94","country_code":"DK","type":"education","lineage":["https://openalex.org/I96673099"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Kirstine Berg-S\u00f8rensen","raw_affiliation_strings":["Denmark","Department of Physics","Technical University of Denmark","Department of Physics, Technical University of Denmark, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Denmark","institution_ids":[]},{"raw_affiliation_string":"Department of Physics","institution_ids":[]},{"raw_affiliation_string":"Technical University of Denmark","institution_ids":["https://openalex.org/I96673099"]},{"raw_affiliation_string":"Department of Physics, Technical University of Denmark, Denmark","institution_ids":["https://openalex.org/I96673099"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070844214","display_name":"Lene Broeng Oddershede","orcid":"https://orcid.org/0000-0003-2923-2844","observed_orcids":["https://orcid.org/0000-0003-2923-2844"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":true,"raw_author_name":"Lene B. Oddershede","raw_affiliation_strings":["Copenhagen, Denmark","Niels Bohr Institute","University of Copenhagen","[Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark]"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Copenhagen, Denmark","institution_ids":[]},{"raw_affiliation_string":"Niels Bohr Institute","institution_ids":[]},{"raw_affiliation_string":"University of Copenhagen","institution_ids":["https://openalex.org/I124055696"]},{"raw_affiliation_string":"[Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark]","institution_ids":["https://openalex.org/I124055696"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5070844214"],"corresponding_institution_ids":["https://openalex.org/I124055696"],"apc_list":null,"apc_paid":null,"fwci":2.9468,"has_fulltext":false,"cited_by_count":42,"citation_normalized_percentile":{"value":0.91571126,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"16","issue":"25","first_page":"12614","last_page":"12624"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10490","display_name":"Orbital Angular Momentum in Optics","score":0.7339000105857849,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10490","display_name":"Orbital Angular Momentum in Optics","score":0.7339000105857849,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.2409999966621399,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.009800000116229057,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/optical-tweezers","display_name":"optical tweezers","score":1.0},{"id":"https://openalex.org/keywords/single-molecule-manipulation","display_name":"single-molecule manipulation","score":0.875},{"id":"https://openalex.org/keywords/force-measurement","display_name":"force measurement","score":0.4869999885559082},{"id":"https://openalex.org/keywords/living-cells","display_name":"living cells","score":0.8140000104904175},{"id":"https://openalex.org/keywords/cytoplasm","display_name":"cytoplasm","score":0.6470000147819519},{"id":"https://openalex.org/keywords/microrheology","display_name":"microrheology","score":0.8220000267028809},{"id":"https://openalex.org/keywords/lipid-granules","display_name":"lipid granules","score":0.9760000109672546},{"id":"https://openalex.org/keywords/chromosomes","display_name":"chromosomes","score":0.9509999752044678},{"id":"https://openalex.org/keywords/optical-trapping","display_name":"optical trapping","score":0.32899999618530273}],"concepts":[{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.5084297060966492},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.40717318654060364},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37393566966056824},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2648668587207794},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08772706985473633}],"mesh":[{"descriptor_ui":"D002138","descriptor_name":"Calibration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002477","descriptor_name":"Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D052898","descriptor_name":"Optical Tweezers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1039/c4cp00208c","doi":"https://doi.org/10.1039/c4cp00208c","is_oa":false,"landing_page_url":"https://doi.org/10.1039/c4cp00208c","pdf_url":null,"source":{"id":"https://openalex.org/S181332294","display_name":"Physical Chemistry Chemical Physics","issn_l":"1463-9076","issn":["1463-9076","1463-9084"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-3","ki-jl-1","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Phys. Chem. Chem. Phys.","raw_type":"journal-article"},{"id":"pmid:24651890","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24651890","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Physical chemistry chemical physics : PCCP","raw_type":"Journal Article"},{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/b5f3dc14-78cf-4aad-bc22-c60f9dc6b55c","doi":null,"is_oa":false,"landing_page_url":"https://curis.ku.dk/portal/da/publications/optical-manipulation-of-single-molecules-in-the-living-cell(b5f3dc14-78cf-4aad-bc22-c60f9dc6b55c).html","pdf_url":null,"source":{"id":"https://openalex.org/S4306401983","display_name":"Research at the University of Copenhagen (University of Copenhagen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I124055696","host_organization_name":"University of Copenhagen","host_organization_lineage":["https://openalex.org/I124055696"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"N\u00f8rregaard, K, Pedersen, L J, Berg-S\u00f8rensen, K & Oddershede, L B 2014, 'Optical manipulation of single molecules in the living cell', Physical Chemistry Chemical Physics, vol. 16, no. 25, pp. 12614-12624. https://doi.org/10.1039/c4cp00208c","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:pure.atira.dk:publications/7fa8da28-3a80-4bc0-80e0-e693a0771732","doi":null,"is_oa":false,"landing_page_url":"https://orbit.dtu.dk/en/publications/7fa8da28-3a80-4bc0-80e0-e693a0771732","pdf_url":null,"source":{"id":"https://openalex.org/S4306400705","display_name":"Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I96673099","host_organization_name":"Technical University of Denmark","host_organization_lineage":["https://openalex.org/I96673099"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Norregaard , K , Jauffred , L , Berg-S\u00f8rensen , K & Oddershede , L B 2014 , ' Optical manipulation of single molecules in the living cell ' , Physical Chemistry Chemical Physics , vol. 16 , no. 25 , pp. 12614-12624 . https://doi.org/10.1039/c4cp00208c","raw_type":"article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":98,"referenced_works":["https://openalex.org/W1487659884","https://openalex.org/W1498593390","https://openalex.org/W1510866292","https://openalex.org/W1515092449","https://openalex.org/W1817473332","https://openalex.org/W1966086662","https://openalex.org/W1968921863","https://openalex.org/W1972398314","https://openalex.org/W1975247969","https://openalex.org/W1975532391","https://openalex.org/W1976765186","https://openalex.org/W1978249718","https://openalex.org/W1979340466","https://openalex.org/W1982557289","https://openalex.org/W1987939090","https://openalex.org/W1990459252","https://openalex.org/W1992858116","https://openalex.org/W1999250123","https://openalex.org/W2000479770","https://openalex.org/W2003617144","https://openalex.org/W2005084201","https://openalex.org/W2007402868","https://openalex.org/W2009744852","https://openalex.org/W2017457274","https://openalex.org/W2019215591","https://openalex.org/W2020010253","https://openalex.org/W2021211941","https://openalex.org/W2022149140","https://openalex.org/W2022475320","https://openalex.org/W2022817606","https://openalex.org/W2027738535","https://openalex.org/W2028568731","https://openalex.org/W2029158435","https://openalex.org/W2030365976","https://openalex.org/W2033257199","https://openalex.org/W2033431258","https://openalex.org/W2033859483","https://openalex.org/W2034735979","https://openalex.org/W2036265613","https://openalex.org/W2037449390","https://openalex.org/W2041105700","https://openalex.org/W2041654055","https://openalex.org/W2042081776","https://openalex.org/W2044100923","https://openalex.org/W2047519015","https://openalex.org/W2052216032","https://openalex.org/W2055566196","https://openalex.org/W2055790819","https://openalex.org/W2057995810","https://openalex.org/W2068524116","https://openalex.org/W2070675925","https://openalex.org/W2072335754","https://openalex.org/W2072903201","https://openalex.org/W2072926767","https://openalex.org/W2074541903","https://openalex.org/W2077961113","https://openalex.org/W2082252496","https://openalex.org/W2087627543","https://openalex.org/W2091464855","https://openalex.org/W2092674833","https://openalex.org/W2098889694","https://openalex.org/W2104425579","https://openalex.org/W2105608576","https://openalex.org/W2111053500","https://openalex.org/W2111299494","https://openalex.org/W2114452066","https://openalex.org/W2116313856","https://openalex.org/W2116479507","https://openalex.org/W2117116692","https://openalex.org/W2122739844","https://openalex.org/W2123498748","https://openalex.org/W2124683597","https://openalex.org/W2128427159","https://openalex.org/W2130379504","https://openalex.org/W2133257760","https://openalex.org/W2138103294","https://openalex.org/W2139279857","https://openalex.org/W2140086723","https://openalex.org/W2140405694","https://openalex.org/W2144879614","https://openalex.org/W2151034868","https://openalex.org/W2152516404","https://openalex.org/W2153375918","https://openalex.org/W2154827556","https://openalex.org/W2156524419","https://openalex.org/W2158190317","https://openalex.org/W2158208080","https://openalex.org/W2158655795","https://openalex.org/W2160708064","https://openalex.org/W2160780620","https://openalex.org/W2165134887","https://openalex.org/W2166009034","https://openalex.org/W2171250826","https://openalex.org/W2171712877","https://openalex.org/W2313159301","https://openalex.org/W2320696217","https://openalex.org/W2320781448","https://openalex.org/W4211178136"],"related_works":["https://openalex.org/W2554474864","https://openalex.org/W3036160793","https://openalex.org/W2584641334","https://openalex.org/W2129917004","https://openalex.org/W2594908770","https://openalex.org/W2549556690","https://openalex.org/W2409368126","https://openalex.org/W2072926767","https://openalex.org/W2388805686","https://openalex.org/W2031179592"],"abstract_inverted_index":{"Optical":[0,65],"tweezers":[1,43,66],"are":[2,44,127],"the":[3,18,26,31,58,62,78,85,95,107,118,121,125],"only":[4],"nano-tools":[5],"capable":[6],"of":[7,61,97,124,143],"manipulating":[8],"and":[9,15,29,50,54,120,130,135],"performing":[10,22],"force-measurements":[11],"on":[12],"individual":[13,48,69,144],"molecules":[14,49,70,145],"organelles":[16],"within":[17,57],"living":[19,63],"cell":[20,27,126],"without":[21,30],"destructive":[23],"penetration":[24],"through":[25,139],"wall":[28],"need":[32],"for":[33,88],"inserting":[34],"a":[35,103],"non-endogenous":[36],"probe.":[37],"Here,":[38],"we":[39,131],"describe":[40],"how":[41],"optical":[42,75,108,140],"used":[45,113],"to":[46,77,102],"manipulate":[47],"perform":[51],"accurate":[52],"force":[53,141],"distance":[55],"measurements":[56],"complex":[59],"cytoplasm":[60],"cell.":[64],"can":[67],"grab":[68],"or":[71,91],"organelles,":[72],"if":[73],"their":[74,115],"contrast":[76],"medium":[79],"is":[80,84,99],"large":[81],"enough,":[82],"as":[83],"case,":[86],"e.g.,":[87],"lipid":[89],"granules":[90],"chromosomes.":[92],"However,":[93],"often":[94],"molecule":[96],"interest":[98],"specifically":[100],"attached":[101],"handle":[104],"manipulated":[105],"by":[106],"trap.":[109],"The":[110],"most":[111],"commonly":[112],"handles,":[114],"insertion":[116],"into":[117],"cytoplasm,":[119],"relevant":[122],"micro-rheology":[123],"discussed":[128],"here":[129],"also":[132],"review":[133],"recent":[134],"exciting":[136],"results":[137],"achieved":[138],"manipulation":[142],"in":[146],"vivo.":[147]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}