{"id":"https://openalex.org/W2057074411","doi":"https://doi.org/10.1172/jci1617","title":"Generation and characterization of mice deficient in hepsin, a hepatic transmembrane serine protease.","display_name":"Generation and characterization of mice deficient in hepsin, a hepatic transmembrane serine protease.","publication_year":1998,"publication_date":"1998-01-15","ids":{"openalex":"https://openalex.org/W2057074411","doi":"https://doi.org/10.1172/jci1617","mag":"2057074411","pmid":"https://pubmed.ncbi.nlm.nih.gov/9435303","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/508570"},"language":"en","primary_location":{"id":"doi:10.1172/jci1617","doi":"https://doi.org/10.1172/jci1617","is_oa":false,"landing_page_url":"https://doi.org/10.1172/jci1617","pdf_url":null,"source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/508570","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061354837","display_name":"Qingyu Wu","orcid":"https://orcid.org/0000-0003-0561-9315","observed_orcids":["https://orcid.org/0000-0003-0561-9315"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Q Wu","raw_affiliation_strings":["Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804, USA. Qingyu_Wu@Berlex.com","Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804, USA. Qingyu_Wu@Berlex.com","institution_ids":[]},{"raw_affiliation_string":"Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040450464","display_name":"Dihua Yu","orcid":"https://orcid.org/0000-0001-6231-9381","observed_orcids":["https://orcid.org/0000-0001-6231-9381"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Yu","raw_affiliation_strings":["BAYER"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BAYER","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"J Post","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Post","raw_affiliation_strings":["BAYER"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BAYER","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042935008","display_name":"Meredith Halks\u2010Miller","orcid":"https://orcid.org/0000-0002-9847-1331","observed_orcids":["https://orcid.org/0000-0002-9847-1331"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Halks-Miller","raw_affiliation_strings":["BAYER"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BAYER","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005704032","display_name":"J. Evan Sadler","orcid":"https://orcid.org/0000-0001-5705-469X","observed_orcids":["https://orcid.org/0000-0001-5705-469X"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J E Sadler","raw_affiliation_strings":["Washington University \u2013 St Louis"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington University \u2013 St Louis","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018985536","display_name":"John Morser","orcid":"https://orcid.org/0000-0003-3376-8692","observed_orcids":["https://orcid.org/0000-0003-3376-8692"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Morser","raw_affiliation_strings":["BAYER"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BAYER","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5200,"currency":"USD","value_usd":5200},"apc_paid":null,"fwci":1.3374,"has_fulltext":false,"cited_by_count":85,"citation_normalized_percentile":{"value":0.79264863,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"101","issue":"2","first_page":"321","last_page":"326"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.9160000085830688,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.9160000085830688,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.0731000006198883,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepsin","display_name":"hepsin","score":1.0},{"id":"https://openalex.org/keywords/transmembrane-serine-protease","display_name":"transmembrane serine protease","score":0.9509999752044678},{"id":"https://openalex.org/keywords/blood-coagulation","display_name":"coagulation","score":0.7770000100135803},{"id":"https://openalex.org/keywords/hemostasis","display_name":"hemostasis","score":0.9399999976158142},{"id":"https://openalex.org/keywords/disseminated-intravascular-coagulation","display_name":"disseminated intravascular coagulation","score":0.9589999914169312},{"id":"https://openalex.org/keywords/tissue-factor","display_name":"tissue factor","score":0.8370000123977661},{"id":"https://openalex.org/keywords/alkaline-phosphatase","display_name":"alkaline phosphatase","score":0.9120000004768372},{"id":"https://openalex.org/keywords/hepatocytes","display_name":"hepatocytes","score":0.6819999814033508},{"id":"https://openalex.org/keywords/embryonic-development","display_name":"embryonic development","score":0.7319999933242798},{"id":"https://openalex.org/keywords/liver-function","display_name":"liver function","score":0.4480000138282776}],"concepts":[{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.6114112138748169},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5129451155662537},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.43834561109542847},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.41020333766937256},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.37089985609054565},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34531503915786743},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23867547512054443},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23682653903961182},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.15274077653884888}],"mesh":[{"descriptor_ui":"D000469","descriptor_name":"Alkaline Phosphatase","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000172","qualifier_name":"deficiency","is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D013925","descriptor_name":"Thromboplastin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D018345","descriptor_name":"Mice, Knockout","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018390","descriptor_name":"Gene Targeting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1172/jci1617","doi":"https://doi.org/10.1172/jci1617","is_oa":false,"landing_page_url":"https://doi.org/10.1172/jci1617","pdf_url":null,"source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:508570","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/508570","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Invest","raw_type":"Text"},{"id":"pmid:9435303","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9435303","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of clinical investigation","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:508570","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/508570","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Invest","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W89574707","https://openalex.org/W139274608","https://openalex.org/W148931160","https://openalex.org/W167520159","https://openalex.org/W202768902","https://openalex.org/W1239656452","https://openalex.org/W1492544320","https://openalex.org/W1508240107","https://openalex.org/W1547743287","https://openalex.org/W1547948326","https://openalex.org/W1594006822","https://openalex.org/W1604192266","https://openalex.org/W1926573163","https://openalex.org/W1949259756","https://openalex.org/W1973404702","https://openalex.org/W1979516833","https://openalex.org/W2005638713","https://openalex.org/W2005782838","https://openalex.org/W2022527563","https://openalex.org/W2027877275","https://openalex.org/W2040862545","https://openalex.org/W2049831508","https://openalex.org/W2060396407","https://openalex.org/W2061460050","https://openalex.org/W2070018377","https://openalex.org/W2081055498","https://openalex.org/W2089509586","https://openalex.org/W2092806325","https://openalex.org/W2093528419","https://openalex.org/W2098267952","https://openalex.org/W2101710059","https://openalex.org/W2112636022","https://openalex.org/W2165868580","https://openalex.org/W2255950219","https://openalex.org/W2299040454","https://openalex.org/W2414944471","https://openalex.org/W2418591689","https://openalex.org/W4230951225","https://openalex.org/W4253629888"],"related_works":["https://openalex.org/W2390105790","https://openalex.org/W1575527051","https://openalex.org/W4246937097","https://openalex.org/W2037562795","https://openalex.org/W1547743287","https://openalex.org/W2060396407","https://openalex.org/W7098203434","https://openalex.org/W4282914412","https://openalex.org/W2139886856","https://openalex.org/W2082080310"],"abstract_inverted_index":{"Hepsin":[0],"is":[1,20,156],"a":[2,31],"type":[3],"II":[4],"transmembrane":[5],"serine":[6],"protease":[7],"highly":[8],"expressed":[9],"on":[10],"the":[11,34,45,171],"surface":[12],"of":[13,18,36,48,102,117,128,174],"hepatocytes.":[14],"The":[15],"physiological":[16],"function":[17],"hepsin":[19,29,155,175],"not":[21,157],"known,":[22],"although":[23],"in":[24,33,40,109,125,142,145,148,177],"vitro":[25],"studies":[26],"indicate":[27,153],"that":[28,154],"plays":[30],"role":[32],"initiation":[35],"blood":[37],"coagulation":[38,84],"and":[39,62,64,77,93,99,111,162],"hepatocyte":[41],"growth.":[42],"To":[43],"determine":[44],"functional":[46,68],"importance":[47],"hepsin,":[49],"we":[50],"generated":[51],"hepsin-deficient":[52],"mice":[53,59,127,147,166],"by":[54],"homologous":[55],"recombination.":[56],"Homozygous":[57],"hepsin-/-":[58,92,110,126,146],"were":[60,107,121,140],"viable":[61],"fertile,":[63],"grew":[65],"normally.":[66],"In":[67],"assays":[69],"including":[70],"tail":[71],"bleeding":[72],"time,":[73],"plasma":[74],"clotting":[75],"times,":[76],"tissue":[78],"factor-":[79],"or":[80,105],"LPS-induced":[81],"disseminated":[82],"intravascular":[83],"models,":[85],"no":[86],"significant":[87],"difference":[88],"was":[89],"found":[90,141],"between":[91],"wild-type":[94,112,134],"litter":[95,135],"mates.":[96,136],"Liver":[97],"weight":[98],"serum":[100,115],"concentrations":[101,116],"liver-derived":[103],"proteins":[104],"enzymes":[106],"similar":[108],"mice.":[113],"Interestingly,":[114],"bone-derived":[118],"alkaline":[119],"phosphatase":[120],"approximately":[122],"twofold":[123],"higher":[124],"both":[129],"sexes":[130],"when":[131],"compared":[132],"with":[133],"No":[137],"obvious":[138],"abnormalities":[139],"major":[143],"organs":[144],"histological":[149],"examinations.":[150],"Our":[151],"results":[152],"essential":[158],"for":[159],"embryonic":[160],"development":[161],"normal":[163],"hemostasis.":[164],"Hepsin-/-":[165],"will":[167],"help":[168],"to":[169],"evaluate":[170],"long-term":[172],"effects":[173],"deficiency":[176],"these":[178],"animals.":[179]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}