{"id":"https://openalex.org/W2056592468","doi":"https://doi.org/10.1086/297315","title":"Apoptosis During Diploid Parthenogenesis and Early Somatic Embryogenesis of Norway Spruce","display_name":"Apoptosis During Diploid Parthenogenesis and Early Somatic Embryogenesis of Norway Spruce","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W2056592468","doi":"https://doi.org/10.1086/297315","mag":"2056592468"},"language":"en","primary_location":{"id":"doi:10.1086/297315","doi":"https://doi.org/10.1086/297315","is_oa":false,"landing_page_url":"https://doi.org/10.1086/297315","pdf_url":null,"source":{"id":"https://openalex.org/S85490461","display_name":"International Journal of Plant Sciences","issn_l":"1058-5893","issn":["1058-5893","1537-5315"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315672","host_organization_name":"University of Chicago Press","host_organization_lineage":["https://openalex.org/P4310315672","https://openalex.org/P4310315690"],"host_organization_lineage_names":["University of Chicago Press","University of Chicago"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Plant Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087419968","display_name":"Ladislav Havel","orcid":"https://orcid.org/0000-0003-3378-2785","observed_orcids":["https://orcid.org/0000-0003-3378-2785"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L. Havel","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5083203892","display_name":"Don J. Durzan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D. J. Durzan","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":9.2829,"has_fulltext":false,"cited_by_count":75,"citation_normalized_percentile":{"value":0.98036411,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"157","issue":"1","first_page":"8","last_page":"16"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.6478999853134155,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.6478999853134155,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.1932000070810318,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.039400000125169754,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.9480000138282776},{"id":"https://openalex.org/keywords/norway-spruce","display_name":"Norway spruce","score":0.9269999861717224},{"id":"https://openalex.org/keywords/somatic-embryogenesis","display_name":"somatic embryogenesis","score":0.8809999823570251},{"id":"https://openalex.org/keywords/suspensor","display_name":"suspensor","score":0.5899999737739563},{"id":"https://openalex.org/keywords/endonuclease-activity","display_name":"endonuclease activity","score":0.7950000166893005},{"id":"https://openalex.org/keywords/tunel-assay","display_name":"TUNEL","score":0.8309999704360962},{"id":"https://openalex.org/keywords/nucleolus","display_name":"nucleolus","score":0.4659999907016754},{"id":"https://openalex.org/keywords/embryonic-survival","display_name":"embryo survival","score":0.4189999997615814}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8828438520431519},{"id":"https://openalex.org/C154113507","wikidata":"https://www.wikidata.org/wiki/Q411149","display_name":"Terminal deoxynucleotidyl transferase","level":4,"score":0.7083415985107422},{"id":"https://openalex.org/C196795494","wikidata":"https://www.wikidata.org/wiki/Q1624817","display_name":"TUNEL assay","level":3,"score":0.6665270924568176},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5531821250915527},{"id":"https://openalex.org/C183873130","wikidata":"https://www.wikidata.org/wiki/Q30869","display_name":"Nucleolus","level":3,"score":0.5499918460845947},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.5155594348907471},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.4736565053462982},{"id":"https://openalex.org/C2780114586","wikidata":"https://www.wikidata.org/wiki/Q40260","display_name":"Cell nucleus","level":3,"score":0.46131962537765503},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.45696136355400085},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.42920365929603577},{"id":"https://openalex.org/C2777527632","wikidata":"https://www.wikidata.org/wiki/Q238382","display_name":"DAPI","level":3,"score":0.42072486877441406},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.39678969979286194},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2925730347633362},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2691638469696045}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1086/297315","doi":"https://doi.org/10.1086/297315","is_oa":false,"landing_page_url":"https://doi.org/10.1086/297315","pdf_url":null,"source":{"id":"https://openalex.org/S85490461","display_name":"International Journal of Plant Sciences","issn_l":"1058-5893","issn":["1058-5893","1537-5315"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315672","host_organization_name":"University of Chicago Press","host_organization_lineage":["https://openalex.org/P4310315672","https://openalex.org/P4310315690"],"host_organization_lineage_names":["University of Chicago Press","University of Chicago"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Plant Sciences","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W46797858","https://openalex.org/W426344629","https://openalex.org/W1481327359","https://openalex.org/W1550426978","https://openalex.org/W1986064635","https://openalex.org/W2007564880","https://openalex.org/W2019417736","https://openalex.org/W2043708408","https://openalex.org/W2052853635","https://openalex.org/W2056471545","https://openalex.org/W2060676319","https://openalex.org/W2069917420","https://openalex.org/W2075926315","https://openalex.org/W2076090031","https://openalex.org/W2084147994","https://openalex.org/W2138302361","https://openalex.org/W2344183540"],"related_works":["https://openalex.org/W2167916354","https://openalex.org/W2019417736","https://openalex.org/W2032058338","https://openalex.org/W3001214487","https://openalex.org/W2054966963","https://openalex.org/W1033174419","https://openalex.org/W2363984586","https://openalex.org/W2792565458","https://openalex.org/W2474891564","https://openalex.org/W2105825262"],"abstract_inverted_index":{"In":[0],"diploid":[1],"parthenogenesis":[2],"of":[3,32,38,42,53,65,77,87,122,158,163,192],"Norway":[4],"spruce,":[5],"specific":[6],"nuclei":[7,97,107,153,191],"were":[8],"ontogenetically":[9],"eliminated":[10],"in":[11,35,62,74,96,105,208],"cells":[12,64,209],"programmed":[13],"for":[14,26,131,142,155],"cell":[15,101],"death":[16],"or":[17],"apoptosis.":[18],"Nuclear":[19],"elimination":[20],"was":[21,69,81,108,167],"defined":[22],"morphologically":[23],"and":[24,34,48,55,98,174,185,188],"biochemically":[25,82],"the":[27,39,51,59,66,75,85,119,156,181,186,193,212],"degenerating":[28],"ventral":[29],"canal":[30],"nucleus":[31],"egg-equivalents,":[33],"differentiating":[36],"suspensors":[37],"axial":[40,67],"tier":[41,68],"early":[43],"embryos.":[44],"Nuclei":[45,202],"became":[46],"pycnotic":[47],"disintegrated":[49],"with":[50,128,133,198,206],"release":[52],"nucleoli":[54],"nuclear":[56,164],"fragments":[57],"into":[58],"cytoplasm.":[60],"Enucleation":[61],"tubular":[63],"preceded":[70],"by":[71,84,110,125,176,211],"a":[72,111,134,139],"reduction":[73],"number":[76],"interphase":[78],"nucleoli.":[79],"Apoptosis":[80],"characterized":[83],"activation":[86],"endonuclease":[88],"activity":[89,104],"that":[90],"contributed":[91],"to":[92,100,169],"massive":[93],"morphological":[94],"changes":[95],"ultimately":[99],"death.":[102],"Endonuclease":[103],"individual":[106],"determined":[109],"terminal":[112],"deoxynucleotidyl":[113],"transferase":[114],"(TdT).":[115],"This":[116],"enzyme":[117],"labeled":[118],"3'OH":[120],"ends":[121],"DNA,":[123],"generated":[124],"DNA":[126],"nicking":[127],"biotin-conjugated":[129],"dUTP,":[130],"visualization":[132],"secondary":[135],"detection":[136],"system":[137],"using":[138],"colorimetric":[140],"substrate":[141],"peroxidase.":[143],"The":[144,161],"TdT-mediated":[145],"dUTP-biotin":[146],"nick-end":[147],"labeling":[148],"(TUNEL)":[149],"assay":[150],"stained":[151],"apoptotic":[152],"red":[154],"expression":[157],"endogenous":[159],"endonucleases.":[160],"salvage":[162],"degradation":[165],"products":[166],"postulated":[168],"enhance":[170],"embryo":[171],"survival,":[172],"growth,":[173],"multiplication":[175],"cleavage":[177],"polyembryony.":[178],"By":[179],"contrast,":[180],"egg-equivalent":[182],"nucleus,":[183],"proembryos,":[184],"large":[187],"rounded":[189],"nonapoptotic":[190],"embryonal":[194],"group":[195],"fluoresced":[196],"strongly":[197],"DAPI":[199],"(4'-6-diamidino-2-phenylindole":[200],"dihydrochloride).":[201],"also":[203],"reacted":[204],"blue":[205],"Giemsa":[207],"probed":[210],"TUNEL":[213],"assay.":[214]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}