{"id":"https://openalex.org/W2056520296","doi":"https://doi.org/10.1263/jbb.106.194","title":"Transient gene expression in guard cell chloroplasts of tobacco using ArF excimer laser microablation","display_name":"Transient gene expression in guard cell chloroplasts of tobacco using ArF excimer laser microablation","publication_year":2008,"publication_date":"2008-08-01","ids":{"openalex":"https://openalex.org/W2056520296","doi":"https://doi.org/10.1263/jbb.106.194","mag":"2056520296","pmid":"https://pubmed.ncbi.nlm.nih.gov/18804064"},"language":"en","primary_location":{"id":"doi:10.1263/jbb.106.194","doi":"https://doi.org/10.1263/jbb.106.194","is_oa":false,"landing_page_url":"https://doi.org/10.1263/jbb.106.194","pdf_url":null,"source":{"id":"https://openalex.org/S63783143","display_name":"Journal of Bioscience and Bioengineering","issn_l":"1347-4421","issn":["1347-4421","1389-1723"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bioscience and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5037814947","display_name":"Shin\u2019ichiro Kajiyama","orcid":"https://orcid.org/0000-0002-6848-4203","observed_orcids":["https://orcid.org/0000-0002-6848-4203"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]},{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shin'Ichiro Kajiyama","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan","Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan","School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan","institution_ids":["https://openalex.org/I98285908"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]},{"raw_affiliation_string":"School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068906179","display_name":"Benesh Joseph","orcid":"https://orcid.org/0000-0003-4968-889X","observed_orcids":["https://orcid.org/0000-0003-4968-889X"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Benesh Joseph","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111491090","display_name":"Fumihide Inoue","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Fumihide Inoue","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068089899","display_name":"Masaki Shimamura","orcid":"https://orcid.org/0000-0002-5665-5116","observed_orcids":["https://orcid.org/0000-0002-5665-5116"]},"institutions":[{"id":"https://openalex.org/I4210145897","display_name":"Research Institute of Innovative Technology for the Earth","ror":"https://ror.org/04p00tq71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210145897"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masaki Shimamura","raw_affiliation_strings":["Research Laboratory for Plant Molecular Physiology, Research Institute of Innovative Technology for the Earth, 9-2 Kizugawa-dai, Kizu-Cho, Soraku-Gun, Kyoto 619-0292, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratory for Plant Molecular Physiology, Research Institute of Innovative Technology for the Earth, 9-2 Kizugawa-dai, Kizu-Cho, Soraku-Gun, Kyoto 619-0292, Japan","institution_ids":["https://openalex.org/I4210145897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066301304","display_name":"Eiichiro Fukusaki","orcid":"https://orcid.org/0000-0003-3069-5547","observed_orcids":["https://orcid.org/0000-0003-3069-5547"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Eiichiro Fukusaki","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113973346","display_name":"Ken\u2010ichi Tomizawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145897","display_name":"Research Institute of Innovative Technology for the Earth","ror":"https://ror.org/04p00tq71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210145897"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenichi Tomizawa","raw_affiliation_strings":["Research Laboratory for Plant Molecular Physiology, Research Institute of Innovative Technology for the Earth, 9-2 Kizugawa-dai, Kizu-Cho, Soraku-Gun, Kyoto 619-0292, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratory for Plant Molecular Physiology, Research Institute of Innovative Technology for the Earth, 9-2 Kizugawa-dai, Kizu-Cho, Soraku-Gun, Kyoto 619-0292, Japan","institution_ids":["https://openalex.org/I4210145897"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111534575","display_name":"Akio Kobayashi","orcid":"https://orcid.org/0009-0000-4302-166X","observed_orcids":["https://orcid.org/0009-0000-4302-166X"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Akio Kobayashi","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5111534575"],"corresponding_institution_ids":["https://openalex.org/I98285908"],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":0.8314,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.66666667,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"106","issue":"2","first_page":"194","last_page":"198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.7773000001907349,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.7773000001907349,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.18799999356269836,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.016899999231100082,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transient-expression","display_name":"transient expression","score":0.9629999995231628},{"id":"https://openalex.org/keywords/chloroplast-transformation","display_name":"chloroplast transformation","score":0.8769999742507935},{"id":"https://openalex.org/keywords/guard-cells","display_name":"guard cells","score":0.878000020980835},{"id":"https://openalex.org/keywords/nicotiana-tabacum","display_name":"tobacco","score":0.9070000052452087},{"id":"https://openalex.org/keywords/gfp-expression","display_name":"GFP expression","score":0.7400000095367432},{"id":"https://openalex.org/keywords/stomatal-closure","display_name":"stomatal closure","score":0.9139999747276306},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.6430000066757202}],"concepts":[{"id":"https://openalex.org/C136547172","wikidata":"https://www.wikidata.org/wiki/Q241368","display_name":"Guard cell","level":2,"score":0.8763033151626587},{"id":"https://openalex.org/C2780477314","wikidata":"https://www.wikidata.org/wiki/Q241056","display_name":"Excimer laser","level":3,"score":0.7787728905677795},{"id":"https://openalex.org/C2780799671","wikidata":"https://www.wikidata.org/wiki/Q17087362","display_name":"Transient (computer programming)","level":2,"score":0.5566669702529907},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.527096152305603},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5219578146934509},{"id":"https://openalex.org/C141141315","wikidata":"https://www.wikidata.org/wiki/Q2379942","display_name":"Guard (computer science)","level":2,"score":0.4944169521331787},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4436694085597992},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4286224842071533},{"id":"https://openalex.org/C74561354","wikidata":"https://www.wikidata.org/wiki/Q901723","display_name":"Excimer","level":3,"score":0.4184058606624603},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.41060787439346313},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.36356258392333984},{"id":"https://openalex.org/C520434653","wikidata":"https://www.wikidata.org/wiki/Q38867","display_name":"Laser","level":2,"score":0.3506328761577606},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3392290472984314},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3111647367477417},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.18769866228103638},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12828412652015686},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.10263532400131226},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0797421932220459},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006046","descriptor_name":"Gold","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010316","descriptor_name":"Particle Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D054018","descriptor_name":"Lasers, Excimer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1263/jbb.106.194","doi":"https://doi.org/10.1263/jbb.106.194","is_oa":false,"landing_page_url":"https://doi.org/10.1263/jbb.106.194","pdf_url":null,"source":{"id":"https://openalex.org/S63783143","display_name":"Journal of Bioscience and Bioengineering","issn_l":"1347-4421","issn":["1347-4421","1389-1723"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bioscience and Bioengineering","raw_type":"journal-article"},{"id":"pmid:18804064","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18804064","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bioscience and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W1506710402","https://openalex.org/W1559267289","https://openalex.org/W1977537416","https://openalex.org/W1983251735","https://openalex.org/W1988531595","https://openalex.org/W2002829704","https://openalex.org/W2005412677","https://openalex.org/W2020986744","https://openalex.org/W2026311140","https://openalex.org/W2032379550","https://openalex.org/W2033975186","https://openalex.org/W2040679387","https://openalex.org/W2049160993","https://openalex.org/W2066811776","https://openalex.org/W2085983043","https://openalex.org/W2089934847","https://openalex.org/W2092164769","https://openalex.org/W2109276758","https://openalex.org/W2114265434","https://openalex.org/W2125091792","https://openalex.org/W2131491171","https://openalex.org/W2138643584","https://openalex.org/W2497617892","https://openalex.org/W2512144930","https://openalex.org/W4285719527","https://openalex.org/W6725434993"],"related_works":["https://openalex.org/W2114265434","https://openalex.org/W2109276758","https://openalex.org/W2137868796","https://openalex.org/W1522228474","https://openalex.org/W2019354226","https://openalex.org/W2073806918","https://openalex.org/W2077264977","https://openalex.org/W2129106971","https://openalex.org/W2022081295","https://openalex.org/W1976252886"],"abstract_inverted_index":null,"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}