{"id":"https://openalex.org/W2056265178","doi":"https://doi.org/10.1016/j.jbiotec.2012.01.028","title":"Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes","display_name":"Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes","publication_year":2012,"publication_date":"2012-02-03","ids":{"openalex":"https://openalex.org/W2056265178","doi":"https://doi.org/10.1016/j.jbiotec.2012.01.028","mag":"2056265178","pmid":"https://pubmed.ncbi.nlm.nih.gov/22326628"},"language":"en","primary_location":{"id":"doi:10.1016/j.jbiotec.2012.01.028","doi":"https://doi.org/10.1016/j.jbiotec.2012.01.028","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jbiotec.2012.01.028","pdf_url":null,"source":{"id":"https://openalex.org/S182099265","display_name":"Journal of Biotechnology","issn_l":"0168-1656","issn":["0168-1656","1873-4863"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056627017","display_name":"Ben Reed","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ben Reed","raw_affiliation_strings":["Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028913983","display_name":"John Blazeck","orcid":"https://orcid.org/0000-0002-6110-2938","observed_orcids":["https://orcid.org/0000-0002-6110-2938"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Blazeck","raw_affiliation_strings":["Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023917549","display_name":"Hal S. Alper","orcid":"https://orcid.org/0000-0002-8246-8605","observed_orcids":["https://orcid.org/0000-0002-8246-8605"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hal Alper","raw_affiliation_strings":["Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States","Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712, United States","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5023917549"],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":{"value":4360,"currency":"USD","value_usd":4360},"apc_paid":null,"fwci":0.899,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.71350648,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"158","issue":"3","first_page":"75","last_page":"79"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.6523000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.6523000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.17260000109672546,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.07680000364780426,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/short-chain-alkanes","display_name":"short-chain alkanes","score":0.8029999732971191},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.6769999861717224},{"id":"https://openalex.org/keywords/error-prone-pcr","display_name":"error-prone PCR","score":0.7310000061988831},{"id":"https://openalex.org/keywords/fluorescence-activated-cell-sorting","display_name":"fluorescence-activated cell sorting","score":0.8529999852180481},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7429999709129333},{"id":"https://openalex.org/keywords/gfp-reporter","display_name":"GFP reporter","score":0.7200000286102295}],"concepts":[{"id":"https://openalex.org/C2781327020","wikidata":"https://www.wikidata.org/wiki/Q41581","display_name":"Alkane","level":3,"score":0.6588257551193237},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.622944176197052},{"id":"https://openalex.org/C2780543182","wikidata":"https://www.wikidata.org/wiki/Q2738168","display_name":"Pseudomonas putida","level":3,"score":0.6204345226287842},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5101154446601868},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.5056262016296387},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49936604499816895},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.48850929737091064},{"id":"https://openalex.org/C2777589835","wikidata":"https://www.wikidata.org/wiki/Q150429","display_name":"Pentane","level":2,"score":0.46391263604164124},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.4341876208782196},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4007995128631592},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39342325925827026},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.36443954706192017},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3088895380496979},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.17368999123573303},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.10708630084991455},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000473","descriptor_name":"Alkanes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000473","descriptor_name":"Alkanes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000473","descriptor_name":"Alkanes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016958","descriptor_name":"Pseudomonas putida","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020125","descriptor_name":"Mutation, Missense","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jbiotec.2012.01.028","doi":"https://doi.org/10.1016/j.jbiotec.2012.01.028","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jbiotec.2012.01.028","pdf_url":null,"source":{"id":"https://openalex.org/S182099265","display_name":"Journal of Biotechnology","issn_l":"0168-1656","issn":["0168-1656","1873-4863"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biotechnology","raw_type":"journal-article"},{"id":"pmid:22326628","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22326628","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biotechnology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310620","display_name":"University of Texas at Austin","ror":"https://ror.org/00hj54h04"},{"id":"https://openalex.org/F4320332414","display_name":"Department of Mechanical Engineering, University of Texas at Austin","ror":"https://ror.org/00hj54h04"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W1493556845","https://openalex.org/W1513717105","https://openalex.org/W1583211798","https://openalex.org/W1596254587","https://openalex.org/W1709457880","https://openalex.org/W1741878792","https://openalex.org/W1748737364","https://openalex.org/W1972306691","https://openalex.org/W1982052751","https://openalex.org/W2003536193","https://openalex.org/W2017204918","https://openalex.org/W2037993016","https://openalex.org/W2057998342","https://openalex.org/W2066461160","https://openalex.org/W2090000449","https://openalex.org/W2111325483","https://openalex.org/W2134783465","https://openalex.org/W2136012241","https://openalex.org/W2143256443","https://openalex.org/W2804007336","https://openalex.org/W2916371305"],"related_works":["https://openalex.org/W2272482044","https://openalex.org/W4320491490","https://openalex.org/W1604051195","https://openalex.org/W4400197464","https://openalex.org/W7064579471","https://openalex.org/W2023308290","https://openalex.org/W1748737364","https://openalex.org/W3197427572","https://openalex.org/W2789503396","https://openalex.org/W3213406240"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}