{"id":"https://openalex.org/W2055760720","doi":"https://doi.org/10.1016/s0014-5793(97)01026-0","title":"In vitro virus: Bonding of mRNA bearing puromycin at the 3\u2032\u2010terminal end to the C\u2010terminal end of its encoded protein on the ribosome in vitro","display_name":"In vitro virus: Bonding of mRNA bearing puromycin at the 3\u2032\u2010terminal end to the C\u2010terminal end of its encoded protein on the ribosome in vitro","publication_year":1997,"publication_date":"1997-09-08","ids":{"openalex":"https://openalex.org/W2055760720","doi":"https://doi.org/10.1016/s0014-5793(97)01026-0","mag":"2055760720","pmid":"https://pubmed.ncbi.nlm.nih.gov/9315729"},"language":"en","primary_location":{"id":"doi:10.1016/s0014-5793(97)01026-0","doi":"https://doi.org/10.1016/s0014-5793(97)01026-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(97)01026-0","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2901026-0","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2901026-0","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007966350","display_name":"Naoto Nemoto","orcid":"https://orcid.org/0009-0000-2038-5145","observed_orcids":["https://orcid.org/0009-0000-2038-5145"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"N Nemoto","raw_affiliation_strings":["Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo, Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012497365","display_name":"Etsuko Miyamoto\u2010Sato","orcid":"https://orcid.org/0000-0002-6289-632X","observed_orcids":["https://orcid.org/0000-0002-6289-632X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E Miyamoto-Sato","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072717552","display_name":"Yuzuru Husimi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y Husimi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111541664","display_name":"Hiroshi Yanagawa","orcid":"https://orcid.org/0009-0000-8557-684X","observed_orcids":["https://orcid.org/0009-0000-8557-684X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Yanagawa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":2.8428,"has_fulltext":true,"cited_by_count":463,"citation_normalized_percentile":{"value":0.91629385,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"414","issue":"2","first_page":"405","last_page":"408"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11764","display_name":"Evolution and Genetic Dynamics","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/puromycin","display_name":"puromycin","score":0.9279999732971191},{"id":"https://openalex.org/keywords/ribosome","display_name":"ribosome","score":0.7649999856948853},{"id":"https://openalex.org/keywords/in-vitro-translation","display_name":"in vitro translation","score":0.7639999985694885},{"id":"https://openalex.org/keywords/genotype-phenotype-correlation","display_name":"genotype-phenotype correlation","score":0.7450000047683716},{"id":"https://openalex.org/keywords/protein-evolution","display_name":"protein evolution","score":0.8889999985694885},{"id":"https://openalex.org/keywords/protein-selection","display_name":"protein selection","score":0.5270000100135803},{"id":"https://openalex.org/keywords/phage-display","display_name":"phage display","score":0.6850000023841858}],"concepts":[{"id":"https://openalex.org/C2779200571","wikidata":"https://www.wikidata.org/wiki/Q424696","display_name":"Puromycin","level":3,"score":0.7810249328613281},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.6730940937995911},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.5908843278884888},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.5396912097930908},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.5305030345916748},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5095787048339844},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.4959307610988617},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4743056297302246},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.45607948303222656},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3748302757740021},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.35845571756362915},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.32827073335647583},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31117066740989685},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2859388589859009},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16412663459777832}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001435","descriptor_name":"Bacteriophages","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005075","descriptor_name":"Biological Evolution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011691","descriptor_name":"Puromycin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012156","descriptor_name":"Reticulocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014644","descriptor_name":"Genetic Variation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014780","descriptor_name":"Viruses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016875","descriptor_name":"tau Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D016875","descriptor_name":"tau Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019281","descriptor_name":"Dimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0014-5793(97)01026-0","doi":"https://doi.org/10.1016/s0014-5793(97)01026-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(97)01026-0","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2901026-0","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:9315729","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9315729","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0014-5793(97)01026-0","doi":"https://doi.org/10.1016/s0014-5793(97)01026-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(97)01026-0","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2901026-0","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2055760720.pdf","grobid_xml":"https://content.openalex.org/works/W2055760720.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W150987474","https://openalex.org/W1554943836","https://openalex.org/W1799375255","https://openalex.org/W1862261356","https://openalex.org/W1970403529","https://openalex.org/W1984286262","https://openalex.org/W1990557050","https://openalex.org/W2006891427","https://openalex.org/W2010649076","https://openalex.org/W2017423606","https://openalex.org/W2028161058","https://openalex.org/W2030189518","https://openalex.org/W2070360032","https://openalex.org/W2077034524","https://openalex.org/W2079760897","https://openalex.org/W2124425166"],"related_works":["https://openalex.org/W1966330764","https://openalex.org/W7097997885","https://openalex.org/W2094258310","https://openalex.org/W1993171676","https://openalex.org/W2028493269","https://openalex.org/W2070569816","https://openalex.org/W34502556","https://openalex.org/W2018380171","https://openalex.org/W67130317","https://openalex.org/W3111501009"],"abstract_inverted_index":{"Adequate":[0],"means":[1],"for":[2,82,98,102],"genotype":[3,27],"assignment":[4],"to":[5,32,61],"phenotype":[6,34],"is":[7,80],"essential":[8],"in":[9,24,42,58,72,77,86],"evolutionary":[10],"molecular":[11],"engineering.":[12],"In":[13],"this":[14,67],"study,":[15],"construction":[16],"of":[17,56],"'in":[18],"vitro":[19,59,78],"virus'":[20],"was":[21,49],"carried":[22],"out":[23],"which":[25],"a":[26,43,54,87,99],"molecule":[28,35],"(mRNA)":[29],"covalently":[30],"binds":[31],"the":[33,40,57,73,91,106],"(protein)":[36],"through":[37],"puromycin":[38],"on":[39],"ribosome":[41],"cell-free":[44],"translation":[45],"system.":[46],"Bonding":[47],"efficiency":[48],"approximately":[50,63],"10%,":[51],"thus":[52],"indicating":[53],"population":[55],"virus":[60,79],"have":[62],"10(12)":[64],"protein":[65,84],"variants,":[66],"number":[68],"being":[69],"10(4)":[70],"that":[71],"phage":[74],"display.":[75],"The":[76],"useful":[81],"examining":[83],"evolution":[85],"test":[88],"tube":[89],"and":[90],"results":[92],"may":[93],"possibly":[94],"serve":[95],"as":[96],"basis":[97],"general":[100],"method":[101],"selecting":[103],"proteins":[104],"possessing":[105],"most":[107],"desirable":[108],"functions.":[109]},"counts_by_year":[{"year":2026,"cited_by_count":18},{"year":2025,"cited_by_count":14},{"year":2024,"cited_by_count":16},{"year":2023,"cited_by_count":15},{"year":2022,"cited_by_count":16},{"year":2021,"cited_by_count":22},{"year":2020,"cited_by_count":20},{"year":2019,"cited_by_count":17},{"year":2018,"cited_by_count":14},{"year":2017,"cited_by_count":19},{"year":2016,"cited_by_count":12},{"year":2015,"cited_by_count":20},{"year":2014,"cited_by_count":17},{"year":2013,"cited_by_count":23},{"year":2012,"cited_by_count":26}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}