{"id":"https://openalex.org/W2055455129","doi":"https://doi.org/10.1089/scd.2006.15.109","title":"Specific and Stable Gene Transfer to Human Embryonic Stem Cells Using Pseudotyped Lentiviral Vectors","display_name":"Specific and Stable Gene Transfer to Human Embryonic Stem Cells Using Pseudotyped Lentiviral Vectors","publication_year":2006,"publication_date":"2006-02-01","ids":{"openalex":"https://openalex.org/W2055455129","doi":"https://doi.org/10.1089/scd.2006.15.109","mag":"2055455129","pmid":"https://pubmed.ncbi.nlm.nih.gov/16522168"},"language":"en","primary_location":{"id":"doi:10.1089/scd.2006.15.109","doi":"https://doi.org/10.1089/scd.2006.15.109","is_oa":false,"landing_page_url":"https://doi.org/10.1089/scd.2006.15.109","pdf_url":null,"source":{"id":"https://openalex.org/S120158623","display_name":"Stem Cells and Development","issn_l":"1525-8165","issn":["1525-8165","1547-3287","1557-8534","2168-6580"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cells and Development","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110643039","display_name":"Jung\u2010Eun Jang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]},{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jung-Eun Jang","raw_affiliation_strings":["Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90027","Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1174212"]},{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]},{"raw_affiliation_string":"Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010407904","display_name":"Kit L. Shaw","orcid":"https://orcid.org/0000-0002-1293-8050","observed_orcids":["https://orcid.org/0000-0002-1293-8050"]},"institutions":[{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kit Shaw","raw_affiliation_strings":["Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100705349","display_name":"Xiaojin Yu","orcid":"https://orcid.org/0000-0002-1952-2576","observed_orcids":["https://orcid.org/0000-0002-1952-2576"]},"institutions":[{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiao-Jin Yu","raw_affiliation_strings":["Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017786784","display_name":"Denise Petersen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Denise Petersen","raw_affiliation_strings":["Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033377588","display_name":"Karen A. Pepper","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karen Pepper","raw_affiliation_strings":["Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021982985","display_name":"Carolyn Lutzko","orcid":"https://orcid.org/0009-0001-6266-3470","observed_orcids":["https://orcid.org/0009-0001-6266-3470"]},"institutions":[{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carolyn Lutzko","raw_affiliation_strings":["Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050971129","display_name":"Donald B. Kohn","orcid":"https://orcid.org/0000-0003-1840-6087","observed_orcids":["https://orcid.org/0000-0003-1840-6087"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]},{"id":"https://openalex.org/I1336910626","display_name":"Children's Hospital Los Angeles","ror":"https://ror.org/00412ts95","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336910626"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald B. Kohn","raw_affiliation_strings":["Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90027","Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1174212"]},{"raw_affiliation_string":"Division of Research Immunology/B.M.T., Children's Hospital Los Angeles, Los Angeles, CA 90027","institution_ids":["https://openalex.org/I1336910626"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.7575,"has_fulltext":false,"cited_by_count":30,"citation_normalized_percentile":{"value":0.93594982,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"15","issue":"1","first_page":"109","last_page":"117"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9901999831199646,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9901999831199646,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.006899999920278788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-embryonic-stem-cells","display_name":"human embryonic stem cells","score":0.9919999837875366},{"id":"https://openalex.org/keywords/lentiviral-vectors","display_name":"lentiviral vectors","score":0.921999990940094},{"id":"https://openalex.org/keywords/pseudotyping","display_name":"pseudotyping","score":0.7860000133514404},{"id":"https://openalex.org/keywords/envelope-glycoprotein","display_name":"envelope glycoprotein","score":0.781000018119812},{"id":"https://openalex.org/keywords/gibbon-ape-leukemia-virus","display_name":"gibbon ape leukemia virus","score":0.8510000109672546},{"id":"https://openalex.org/keywords/vsv-g","display_name":"vesicular stomatitis virus glycoprotein","score":0.7250000238418579},{"id":"https://openalex.org/keywords/murine-embryonic-fibroblasts","display_name":"murine embryonic fibroblasts","score":0.9169999957084656},{"id":"https://openalex.org/keywords/stable-gene-transfer","display_name":"stable gene transfer","score":0.8790000081062317},{"id":"https://openalex.org/keywords/gene-expression-stability","display_name":"gene expression stability","score":0.4090000092983246},{"id":"https://openalex.org/keywords/differentiation","display_name":"differentiation","score":0.39100000262260437}],"concepts":[{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.8731781244277954},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8621169328689575},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.7887309789657593},{"id":"https://openalex.org/C2779057330","wikidata":"https://www.wikidata.org/wiki/Q162762","display_name":"Vesicular stomatitis virus","level":3,"score":0.7667102813720703},{"id":"https://openalex.org/C2779562191","wikidata":"https://www.wikidata.org/wiki/Q673848","display_name":"Murine leukemia virus","level":3,"score":0.694844663143158},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.647586464881897},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5058310031890869},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.4766814708709717},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.46888065338134766},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4399978220462799},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.39808350801467896},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.3797096610069275},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3439787030220032},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.315693736076355},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22701549530029297},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.05899152159690857},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015778","descriptor_name":"Minor Histocompatibility Antigens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D026962","descriptor_name":"Amino Acid Transport System ASC","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1089/scd.2006.15.109","doi":"https://doi.org/10.1089/scd.2006.15.109","is_oa":false,"landing_page_url":"https://doi.org/10.1089/scd.2006.15.109","pdf_url":null,"source":{"id":"https://openalex.org/S120158623","display_name":"Stem Cells and Development","issn_l":"1525-8165","issn":["1525-8165","1547-3287","1557-8534","2168-6580"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cells and Development","raw_type":"journal-article"},{"id":"pmid:16522168","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16522168","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem cells and development","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2019920222","display_name":"GENE EXPRESSION IN BETA-CELLS BY LENTIVIRAL VECTORS","funder_award_id":"R21DK062649","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1601995899","https://openalex.org/W1686812758","https://openalex.org/W1908249423","https://openalex.org/W1992923049","https://openalex.org/W2003611043","https://openalex.org/W2006519535","https://openalex.org/W2009761723","https://openalex.org/W2035595162","https://openalex.org/W2052337124","https://openalex.org/W2065341945","https://openalex.org/W2080589169","https://openalex.org/W2082139926","https://openalex.org/W2110301828","https://openalex.org/W2112936117","https://openalex.org/W2125403231","https://openalex.org/W2160266371","https://openalex.org/W2161949020"],"related_works":["https://openalex.org/W2011648833","https://openalex.org/W1188093619","https://openalex.org/W1569557718","https://openalex.org/W3085175179","https://openalex.org/W2405921529","https://openalex.org/W1992923049","https://openalex.org/W2188944408","https://openalex.org/W1982321638","https://openalex.org/W2416053588","https://openalex.org/W2167958044"],"abstract_inverted_index":{"Genetic":[0],"modification":[1,173],"of":[2,59,66,76,155,174],"human":[3],"embryonic":[4,50],"stem":[5],"cells":[6],"(hESCs)":[7],"is":[8],"an":[9],"important":[10],"tool":[11],"for":[12,38,167],"understanding":[13],"and":[14,79,101,170],"influencing":[15],"their":[16,103],"biologic":[17],"properties.":[18],"At":[19],"the":[20,27,56,68,109,113,120],"present":[21],"time,":[22],"lentiviral":[23,83,148,161],"vectors":[24,84,106,149,162],"pseudotyped":[25,85,107,134,147,160],"with":[26,86],"vesicular":[28],"stomatitis":[29],"virus":[30,117,124],"G":[31],"protein":[32],"(VSV-G)":[33],"have":[34,92,96],"been":[35,93],"most":[36],"effective":[37],"stable":[39,171],"gene":[40,77,143,172],"transfer":[41,78],"to":[42,54,95,129],"hESCs.":[43,175],"However,":[44],"they":[45],"also":[46],"efficiently":[47],"transduce":[48,139],"murine":[49],"fibroblasts":[51],"(MEF),":[52],"used":[53,62],"support":[55],"undifferentiated":[57],"state":[58],"many":[60],"commonly":[61],"hESC":[63],"lines.":[64],"Transduction":[65],"both":[67],"MEF":[69],"as":[70,72,132],"well":[71],"hESCs":[73,128,156],"complicates":[74],"analyses":[75],"expression.":[80],"We":[81],"made":[82],"envelope":[87],"glycoproteins":[88],"from":[89,111],"retroviruses":[90],"that":[91],"shown":[94],"more":[97],"restricted":[98],"transduction":[99],"ranges":[100],"evaluated":[102],"specificity.":[104],"Lentiviral":[105],"by":[108,145],"envelopes":[110],"either":[112],"gibbon":[114],"ape":[115],"leukemia":[116],"(GALV)":[118],"or":[119],"RD114":[121],"feline":[122],"endogenous":[123],"(RD114)":[125],"specifically":[126],"transduced":[127],"similar":[130],"extents":[131],"VSV-G":[133],"vectors,":[135],"but":[136],"did":[137],"not":[138],"MEF.":[140],"In":[141],"addition,":[142],"modfication":[144],"these":[146],"was":[150],"stably":[151],"maintained":[152],"throughout":[153],"differentiation":[154],"in":[157],"vitro.":[158],"These":[159],"may":[163],"be":[164],"valuable":[165],"tools":[166],"efficient,":[168],"specific":[169]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}