{"id":"https://openalex.org/W2055046464","doi":"https://doi.org/10.1167/iovs.05-1670","title":"Human Blue-Opsin Promoter Preferentially Targets Reporter Gene Expression to Rat S-Cone Photoreceptors","display_name":"Human Blue-Opsin Promoter Preferentially Targets Reporter Gene Expression to Rat S-Cone Photoreceptors","publication_year":2006,"publication_date":"2006-07-28","ids":{"openalex":"https://openalex.org/W2055046464","doi":"https://doi.org/10.1167/iovs.05-1670","mag":"2055046464","pmid":"https://pubmed.ncbi.nlm.nih.gov/16877422"},"language":"en","primary_location":{"id":"doi:10.1167/iovs.05-1670","doi":"https://doi.org/10.1167/iovs.05-1670","is_oa":false,"landing_page_url":"https://doi.org/10.1167/iovs.05-1670","pdf_url":null,"source":{"id":"https://openalex.org/S76304953","display_name":"Investigative Ophthalmology & Visual Science","issn_l":"0146-0404","issn":["0146-0404","1552-5783"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310321950","host_organization_name":"Cadmus Press","host_organization_lineage":["https://openalex.org/P4310321950"],"host_organization_lineage_names":["Cadmus Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative Opthalmology & Visual Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113850024","display_name":"Lyudmyla G. Glushakova","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lyudmyla G. Glushakova","raw_affiliation_strings":["From the Departments of Ophthalmology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Departments of Ophthalmology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111408973","display_name":"Adrian M. Timmers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800717037","display_name":"University of Florida Health","ror":"https://ror.org/04tk2gy88","country_code":"US","type":"education","lineage":["https://openalex.org/I2800717037","https://openalex.org/I33213144"]},{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adrian M. Timmers","raw_affiliation_strings":["From the Departments of Ophthalmology and2Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, Florida","From the Departments of Ophthalmology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Departments of Ophthalmology and2Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, Florida","institution_ids":["https://openalex.org/I2800717037","https://openalex.org/I33213144"]},{"raw_affiliation_string":"From the Departments of Ophthalmology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001100231","display_name":"Jijing Pang","orcid":"https://orcid.org/0000-0002-9235-7909","observed_orcids":["https://orcid.org/0000-0002-9235-7909"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jijing Pang","raw_affiliation_strings":["From the Departments of Ophthalmology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Departments of Ophthalmology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041808438","display_name":"Jacqueline T. Teusner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jacqueline T. Teusner","raw_affiliation_strings":["From the Departments of Ophthalmology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Departments of Ophthalmology and","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086264524","display_name":"William W. Hauswirth","orcid":"https://orcid.org/0000-0002-3244-4947","observed_orcids":["https://orcid.org/0000-0002-3244-4947"]},"institutions":[{"id":"https://openalex.org/I2800717037","display_name":"University of Florida Health","ror":"https://ror.org/04tk2gy88","country_code":"US","type":"education","lineage":["https://openalex.org/I2800717037","https://openalex.org/I33213144"]},{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William W. Hauswirth","raw_affiliation_strings":["From the Departments of Ophthalmology and4Molecular Genetics and the2Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, Florida","From the Departments of Ophthalmology and","Molecular Genetics and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Departments of Ophthalmology and4Molecular Genetics and the2Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, Florida","institution_ids":["https://openalex.org/I2800717037","https://openalex.org/I33213144"]},{"raw_affiliation_string":"From the Departments of Ophthalmology and","institution_ids":[]},{"raw_affiliation_string":"Molecular Genetics and the","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1850,"currency":"USD","value_usd":1850},"apc_paid":null,"fwci":2.1736,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.86266939,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":97},"biblio":{"volume":"47","issue":"8","first_page":"3505","last_page":"3505"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.5640000104904175,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.5640000104904175,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.4108999967575073,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.01119999960064888,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aav5","display_name":"AAV5","score":0.609000027179718},{"id":"https://openalex.org/keywords/aav2","display_name":"AAV2","score":0.9179999828338623},{"id":"https://openalex.org/keywords/s-cone-photoreceptors","display_name":"S-cone photoreceptors","score":0.8190000057220459},{"id":"https://openalex.org/keywords/subretinal-gene-therapy","display_name":"subretinal gene therapy","score":0.7549999952316284},{"id":"https://openalex.org/keywords/gfp-expression","display_name":"GFP expression","score":0.6320000290870667},{"id":"https://openalex.org/keywords/s-opsin","display_name":"S-opsin","score":0.6959999799728394},{"id":"https://openalex.org/keywords/m-opsin","display_name":"M-opsin","score":0.9049999713897705},{"id":"https://openalex.org/keywords/retinal-photoreceptors","display_name":"retinal photoreceptors","score":0.652999997138977},{"id":"https://openalex.org/keywords/transduction-efficiency","display_name":"transduction efficiency","score":0.6639999747276306},{"id":"https://openalex.org/keywords/long-term-transgene-expression","display_name":"long-term transgene expression","score":0.6159999966621399},{"id":"https://openalex.org/keywords/rat-retina","display_name":"rat retina","score":0.6729999780654907}],"concepts":[{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.8944734334945679},{"id":"https://openalex.org/C30028475","wikidata":"https://www.wikidata.org/wiki/Q24785022","display_name":"Opsin","level":4,"score":0.846455454826355},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7361998558044434},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.73297518491745},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.7020305395126343},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.571107029914856},{"id":"https://openalex.org/C4224716","wikidata":"https://www.wikidata.org/wiki/Q10859258","display_name":"Immunostaining","level":3,"score":0.5040978193283081},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.46402767300605774},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.40416383743286133},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3969508707523346},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.23204389214515686},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.17098277807235718},{"id":"https://openalex.org/C202033177","wikidata":"https://www.wikidata.org/wiki/Q423107","display_name":"Rhodopsin","level":3,"score":0.1279156506061554},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12751898169517517},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07198622822761536},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.049960941076278687}],"mesh":[{"descriptor_ui":"D000229","descriptor_name":"Dependovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017299","descriptor_name":"Rod Opsins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017299","descriptor_name":"Rod Opsins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017949","descriptor_name":"Retinal Cone Photoreceptor Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D017949","descriptor_name":"Retinal Cone Photoreceptor Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018390","descriptor_name":"Gene Targeting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1167/iovs.05-1670","doi":"https://doi.org/10.1167/iovs.05-1670","is_oa":false,"landing_page_url":"https://doi.org/10.1167/iovs.05-1670","pdf_url":null,"source":{"id":"https://openalex.org/S76304953","display_name":"Investigative Ophthalmology & Visual Science","issn_l":"0146-0404","issn":["0146-0404","1552-5783"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310321950","host_organization_name":"Cadmus Press","host_organization_lineage":["https://openalex.org/P4310321950"],"host_organization_lineage_names":["Cadmus Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative Opthalmology & Visual Science","raw_type":"journal-article"},{"id":"pmid:16877422","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16877422","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1127293759","display_name":null,"funder_award_id":"EY-07864","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G1981988940","display_name":null,"funder_award_id":"EY 08571","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G2995247961","display_name":null,"funder_award_id":"NS 36302","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G3793359605","display_name":"AUTOSOMAL DOMINANT EYE DISEASE: CATALYTIC RNA TREATMENT","funder_award_id":"R01EY011596","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G4282891850","display_name":"PHOTORECEPTOR GENE EXPRESSION DURING DEVELOPMENT","funder_award_id":"5r01ey007864-10","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G4779980729","display_name":"Adeno-Associated Virus Gene Transfer to Nervous System","funder_award_id":"P01NS036302","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G5262378246","display_name":"VISION RESEARCH CORE","funder_award_id":"P30EY008571","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G5502197003","display_name":null,"funder_award_id":"EY-11596","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G686859034","display_name":"Retinal Gene Delivery by Adeno-Associated Virus","funder_award_id":"R01EY011123","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G823154836","display_name":null,"funder_award_id":"EY-11123","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":57,"referenced_works":["https://openalex.org/W54838897","https://openalex.org/W80513504","https://openalex.org/W239276889","https://openalex.org/W1490065438","https://openalex.org/W1490724066","https://openalex.org/W1525882285","https://openalex.org/W1578653675","https://openalex.org/W1597109281","https://openalex.org/W1732752544","https://openalex.org/W1961457995","https://openalex.org/W1967195106","https://openalex.org/W1968703962","https://openalex.org/W1972704004","https://openalex.org/W1976540547","https://openalex.org/W1976833281","https://openalex.org/W1982498248","https://openalex.org/W1992618284","https://openalex.org/W1996364002","https://openalex.org/W2004228177","https://openalex.org/W2006377024","https://openalex.org/W2014348752","https://openalex.org/W2015368612","https://openalex.org/W2019875735","https://openalex.org/W2028868015","https://openalex.org/W2045966455","https://openalex.org/W2048008983","https://openalex.org/W2048481264","https://openalex.org/W2049492636","https://openalex.org/W2055717293","https://openalex.org/W2059344357","https://openalex.org/W2063640158","https://openalex.org/W2066556283","https://openalex.org/W2070162220","https://openalex.org/W2070394325","https://openalex.org/W2070909384","https://openalex.org/W2072414883","https://openalex.org/W2074518631","https://openalex.org/W2078320036","https://openalex.org/W2099545629","https://openalex.org/W2103598247","https://openalex.org/W2108188137","https://openalex.org/W2113963038","https://openalex.org/W2119207529","https://openalex.org/W2119478711","https://openalex.org/W2126487187","https://openalex.org/W2127724565","https://openalex.org/W2134168057","https://openalex.org/W2136302254","https://openalex.org/W2140092254","https://openalex.org/W2145527032","https://openalex.org/W2148831368","https://openalex.org/W2155015939","https://openalex.org/W2156862290","https://openalex.org/W2157191738","https://openalex.org/W2157773282","https://openalex.org/W2167745656","https://openalex.org/W2172036681"],"related_works":["https://openalex.org/W2083117420","https://openalex.org/W4234723689","https://openalex.org/W1979363758","https://openalex.org/W1409205915","https://openalex.org/W1991157268","https://openalex.org/W2045168845","https://openalex.org/W7099208352","https://openalex.org/W1986640715","https://openalex.org/W2018152057","https://openalex.org/W2264231196"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"develop":[2],"a":[3,28,341],"gene":[4,268],"therapy":[5],"system":[6,334],"that":[7,151,199,241,280,308],"specifically":[8],"targets":[9],"transgene":[10,303,325,348],"expression":[11,110,150,165,179,269,284,328,349],"to":[12,33,79,95,169,270,305,311,323],"S-cones":[13],"of":[14,27,39,59,74,108,148,159,171,213,224,233,246,264,314],"the":[15,18,25,40,60,104,114,157,160,214,225,242,261,287,301,315,318],"mammalian":[16],"retina,":[17],"authors":[19],"coupled":[20],"recombinant":[21],"AAV-mediated":[22],"delivery":[23,326],"with":[24,86,129,146,174,190,273],"use":[26],"human":[29,50,288],"blue-opsin":[30],"(HB)":[31],"promoter":[32,42,162,319],"drive":[34],"expression.":[35],"METHODS:":[36],"Two":[37],"regions":[38],"HB":[41,161],"sequence,":[43],"HB569":[44],"and":[45,66,70,103,106,121,195,209,300,327],"HB996,":[46],"were":[47,83,91,111],"amplified":[48],"from":[49],"DNA,":[51],"cloned":[52],"into":[53,68,260],"an":[54,130,274],"AAV":[55,316],"vector":[56,258],"cassette":[57],"upstream":[58],"green":[61],"fluorescent":[62],"protein":[63],"(GFP)":[64],"gene,":[65],"packaged":[67],"AAV2":[69],"AAV5":[71],"capsids.":[72],"Eyes":[73],"postnatal":[75],"day":[76],"(P)":[77],"40":[78],"P48":[80],"Sprague-Dawley":[81,265],"rats":[82,266],"subretinally":[84],"injected":[85,259],"2":[87,94,137],"muL":[88],"vector.":[89,138,176],"Animals":[90],"humanely":[92],"killed":[93],"3":[96],"weeks":[97],"or":[98,192,203],"20":[99,185,295],"months":[100],"after":[101],"injection,":[102],"pattern":[105],"persistence":[107],"GFP":[109,149,178,188,302],"analyzed":[112],"in":[113,309,329,340,344],"treated":[115,173],"retinas":[116,172],"by":[117,286],"immunohistochemistry,":[118],"Western":[119],"blotting,":[120],"RT-PCR.":[122],"RESULTS:":[123],"AAV5.HB.GFP":[124,140,247,257],"vectors":[125,141,248],"targeted":[126,267],"photoreceptor":[127,271],"transduction":[128,144,244],"efficiency":[131,245,275],"20-fold":[132,277],"higher":[133,278],"than":[134,279],"analogous":[135],"serotype":[136],"Both":[139],"exhibited":[142],"similar":[143],"efficiencies":[145],"patterns":[147],"did":[152],"not":[153],"vary":[154],"depending":[155],"on":[156],"size":[158],"used.":[163],"Transgene":[164,283],"was":[166,180,239,249],"exclusively":[167],"localized":[168],"photoreceptors":[170],"either":[175],"Furthermore,":[177],"observed":[181],"for":[182,252,281,294],"at":[183,292],"least":[184,293],"months.":[186,296],"Dual":[187],"immunostaining":[189],"S-":[191],"M-opsin":[193],"antibodies":[194],"GFP/PNA":[196],"labeling":[197],"revealed":[198],"cones":[200,229,253],"coexpressing":[201,298],"S-opsin/GFP":[202],"M-opsin/GFP":[204],"constituted":[205,220],"37.5%":[206],"+/-":[207,211,222],"8%":[208],"13.5%":[210],"3%":[212],"GFP-positive":[215,226],"photoreceptors,":[216,237],"respectively,":[217],"whereas":[218],"rods":[219],"49%":[221],"5%":[223],"photoreceptors.":[227],"Because":[228],"constitute":[230],"approximately":[231,250,276],"1%":[232],"adult":[234],"rat":[235],"retinal":[236,331],"it":[238],"estimated":[240],"relative":[243],"100:1":[251],"versus":[254],"rods.":[255],"CONCLUSIONS:":[256],"subretinal":[262],"space":[263],"cells":[272],"AAV2.HB.GFP.":[282],"regulated":[285],"blue":[289],"cone-promoter":[290],"persisted":[291],"Cones":[297],"S-opsin":[299],"appeared":[304],"prevail,":[306],"confirming":[307],"addition":[310],"having":[312],"properties":[313],"serotype,":[317],"choice":[320],"is":[321,350],"key":[322],"fine-tuning":[324],"specific":[330],"cells.":[332],"The":[333],"described":[335],"here":[336],"may":[337],"be":[338],"effective":[339],"therapeutic":[342],"setting":[343],"which":[345],"strong":[346],"S-cone":[347],"required.":[351]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}