{"id":"https://openalex.org/W2054975504","doi":"https://doi.org/10.1016/j.ymeth.2008.09.016","title":"Imaging a target of Ca2+ signalling: Dense core granule exocytosis viewed by total internal reflection fluorescence microscopy","display_name":"Imaging a target of Ca2+ signalling: Dense core granule exocytosis viewed by total internal reflection fluorescence microscopy","publication_year":2008,"publication_date":"2008-10-15","ids":{"openalex":"https://openalex.org/W2054975504","doi":"https://doi.org/10.1016/j.ymeth.2008.09.016","mag":"2054975504","pmid":"https://pubmed.ncbi.nlm.nih.gov/18854212","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2597054"},"language":"en","primary_location":{"id":"doi:10.1016/j.ymeth.2008.09.016","doi":"https://doi.org/10.1016/j.ymeth.2008.09.016","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.ymeth.2008.09.016","pdf_url":"https://www.sciencedirect.com/science/article/pii/S1046202308001680/pdf","source":{"id":"https://openalex.org/S73539393","display_name":"Methods","issn_l":"1046-2023","issn":["1046-2023","1095-9130"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://www.sciencedirect.com/science/article/pii/S1046202308001680/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079310385","display_name":"Magalie A. Ravier","orcid":"https://orcid.org/0000-0001-6607-6559","observed_orcids":["https://orcid.org/0000-0001-6607-6559"]},"institutions":[{"id":"https://openalex.org/I95674353","display_name":"UCLouvain","ror":"https://ror.org/02495e989","country_code":"BE","type":"education","lineage":["https://openalex.org/I95674353"]},{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Magalie A. Ravier","raw_affiliation_strings":["Unit of Endocrinology and Metabolism, University of Louvain Faculty of Medicine, UCL 55.30, Avenue Hippocrate 55, B-1200 Brussels, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Unit of Endocrinology and Metabolism, University of Louvain Faculty of Medicine, UCL 55.30, Avenue Hippocrate 55, B-1200 Brussels, Belgium","institution_ids":["https://openalex.org/I95674353","https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080968511","display_name":"Takashi Tsuboi","orcid":"https://orcid.org/0000-0001-8290-2021","observed_orcids":["https://orcid.org/0000-0001-8290-2021"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takashi Tsuboi","raw_affiliation_strings":["Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1, Komaba, Meguro, Tokyo 153-8902, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1, Komaba, Meguro, Tokyo 153-8902, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015737554","display_name":"Guy A. Rutter","orcid":"https://orcid.org/0000-0001-6360-0343","observed_orcids":["https://orcid.org/0000-0001-6360-0343"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Guy A. Rutter","raw_affiliation_strings":["Department of Cell Biology, Division of Medicine, Faculty of Medicine, Sir Alexander Fleming Building, Imperial College London, Exhibition Road, London SW7 2AZ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Division of Medicine, Faculty of Medicine, Sir Alexander Fleming Building, Imperial College London, Exhibition Road, London SW7 2AZ, UK","institution_ids":["https://openalex.org/I47508984"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5015737554"],"corresponding_institution_ids":["https://openalex.org/I47508984"],"apc_list":{"value":3600,"currency":"USD","value_usd":3600},"apc_paid":{"value":3600,"currency":"USD","value_usd":3600},"fwci":1.3568,"has_fulltext":true,"cited_by_count":25,"citation_normalized_percentile":{"value":0.79326923,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"46","issue":"3","first_page":"233","last_page":"238"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9053000211715698,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9053000211715698,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.08609999716281891,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/calcium-signaling","display_name":"calcium signaling","score":0.7179999947547913},{"id":"https://openalex.org/keywords/regulated-secretion","display_name":"regulated secretion","score":0.7919999957084656},{"id":"https://openalex.org/keywords/total-internal-reflection-fluorescence-microscopy","display_name":"total internal reflection fluorescence microscopy","score":0.9739999771118164},{"id":"https://openalex.org/keywords/optical-sectioning","display_name":"optical sectioning","score":0.7879999876022339},{"id":"https://openalex.org/keywords/hormone-secretion","display_name":"hormone secretion","score":0.7889999747276306},{"id":"https://openalex.org/keywords/confocal-microscopy-comparison","display_name":"confocal microscopy comparison","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C121381206","wikidata":"https://www.wikidata.org/wiki/Q902514","display_name":"Total internal reflection fluorescence microscope","level":3,"score":0.9007924199104309},{"id":"https://openalex.org/C175969161","wikidata":"https://www.wikidata.org/wiki/Q323426","display_name":"Exocytosis","level":3,"score":0.8367775678634644},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5721240639686584},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5382905006408691},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5245026350021362},{"id":"https://openalex.org/C49760210","wikidata":"https://www.wikidata.org/wiki/Q5596727","display_name":"Granule (geology)","level":2,"score":0.49190449714660645},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43033766746520996},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4076923727989197},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3213287591934204},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.25637322664260864},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.25015103816986084},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24229282140731812},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.23287737369537354},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1526993215084076},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.12866544723510742},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005089","descriptor_name":"Exocytosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016716","descriptor_name":"PC12 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020013","descriptor_name":"Calcium Signaling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.ymeth.2008.09.016","doi":"https://doi.org/10.1016/j.ymeth.2008.09.016","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.ymeth.2008.09.016","pdf_url":"https://www.sciencedirect.com/science/article/pii/S1046202308001680/pdf","source":{"id":"https://openalex.org/S73539393","display_name":"Methods","issn_l":"1046-2023","issn":["1046-2023","1095-9130"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2597054","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2597054","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods","raw_type":"Text"},{"id":"pmid:18854212","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18854212","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods (San Diego, Calif.)","raw_type":"Journal Article"},{"id":"pmh:oai:dial.uclouvain.be:boreal:19642","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/2078.1/19642","pdf_url":null,"source":{"id":"https://openalex.org/S4306400166","display_name":"Digital Access to Libraries","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2800384882","host_organization_name":"Harris County Public Library","host_organization_lineage":["https://openalex.org/I2800384882"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Methods (San Diego, Calif.), Vol. 46, no. 3, p. 233-8 (2008)","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/j.ymeth.2008.09.016","doi":"https://doi.org/10.1016/j.ymeth.2008.09.016","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.ymeth.2008.09.016","pdf_url":"https://www.sciencedirect.com/science/article/pii/S1046202308001680/pdf","source":{"id":"https://openalex.org/S73539393","display_name":"Methods","issn_l":"1046-2023","issn":["1046-2023","1095-9130"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311904","display_name":"Wellcome Trust","ror":"https://ror.org/029chgv08"},{"id":"https://openalex.org/F4320320065","display_name":"Diabetes UK","ror":"https://ror.org/050rgn017"},{"id":"https://openalex.org/F4320320283","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10"},{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320323467","display_name":"Mochida Memorial Foundation for Medical and Pharmaceutical Research","ror":"https://ror.org/045p8e908"},{"id":"https://openalex.org/F4320327424","display_name":"Research Foundation for Opto-Science and Technology","ror":"https://ror.org/01fvvv243"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320334626","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517"},{"id":"https://openalex.org/F4320335125","display_name":"RIKEN","ror":"https://ror.org/01sjwvz98"},{"id":"https://openalex.org/F4320337741","display_name":"RIKEN Brain Science Institute","ror":"https://ror.org/04j1n1c04"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2054975504.pdf","grobid_xml":"https://content.openalex.org/works/W2054975504.grobid-xml"},"referenced_works_count":58,"referenced_works":["https://openalex.org/W109599291","https://openalex.org/W148837820","https://openalex.org/W1486302232","https://openalex.org/W1539090868","https://openalex.org/W1558787692","https://openalex.org/W1599277155","https://openalex.org/W1976136773","https://openalex.org/W1989965174","https://openalex.org/W2000353267","https://openalex.org/W2000654807","https://openalex.org/W2001590529","https://openalex.org/W2010017695","https://openalex.org/W2011654614","https://openalex.org/W2013173675","https://openalex.org/W2015426350","https://openalex.org/W2016166286","https://openalex.org/W2017427555","https://openalex.org/W2018456874","https://openalex.org/W2018579546","https://openalex.org/W2021749851","https://openalex.org/W2030713769","https://openalex.org/W2032074620","https://openalex.org/W2032571240","https://openalex.org/W2033866317","https://openalex.org/W2036145275","https://openalex.org/W2036853230","https://openalex.org/W2037887026","https://openalex.org/W2039210066","https://openalex.org/W2042381753","https://openalex.org/W2051881543","https://openalex.org/W2065113965","https://openalex.org/W2067542515","https://openalex.org/W2081866534","https://openalex.org/W2085581680","https://openalex.org/W2091697443","https://openalex.org/W2092327340","https://openalex.org/W2098947846","https://openalex.org/W2102781049","https://openalex.org/W2105955034","https://openalex.org/W2107847399","https://openalex.org/W2114033090","https://openalex.org/W2122776408","https://openalex.org/W2127068865","https://openalex.org/W2129963016","https://openalex.org/W2130258021","https://openalex.org/W2133916339","https://openalex.org/W2146072506","https://openalex.org/W2156270295","https://openalex.org/W2158081658","https://openalex.org/W2161127226","https://openalex.org/W2161231472","https://openalex.org/W2163967697","https://openalex.org/W2170850787","https://openalex.org/W2339049224","https://openalex.org/W2464889532","https://openalex.org/W2887036954","https://openalex.org/W4253422011","https://openalex.org/W4255760236"],"related_works":["https://openalex.org/W2429471876","https://openalex.org/W2107847399","https://openalex.org/W3040279284","https://openalex.org/W2010017695","https://openalex.org/W4303432641","https://openalex.org/W2071327366","https://openalex.org/W7097822928","https://openalex.org/W1978325778","https://openalex.org/W7006193005","https://openalex.org/W1909467843"],"abstract_inverted_index":{"Ca2+":[0,40],"ions":[1],"are":[2],"the":[3,95,99,102,115,130],"most":[4],"ubiquitous":[5],"second":[6],"messenger":[7],"found":[8],"in":[9,17],"all":[10],"cells,":[11],"and":[12,25,101,126,137,143],"play":[13],"a":[14,37,92,108],"significant":[15],"role":[16],"controlling":[18],"regulated":[19,35,78],"secretion":[20,125],"from":[21],"neurons,":[22],"endocrine,":[23],"neuroendocrine":[24],"exocrine":[26],"cells.":[27],"Here,":[28],"we":[29],"describe":[30],"microscopic":[31],"techniques":[32],"to":[33,104,123,128],"image":[34],"secretion,":[36],"target":[38],"of":[39,44,65,77,84,94],"signalling.":[41],"The":[42],"first":[43],"these,":[45],"total":[46],"internal":[47],"reflection":[48],"fluorescence":[49,66,110],"(TIRF),":[50],"is":[51,71,87,146],"well":[52,90],"suited":[53],"for":[54,75,98],"optical":[55],"sectioning":[56],"at":[57],"cell-substrate":[58],"regions":[59],"with":[60],"an":[61],"unusually":[62],"thin":[63],"region":[64],"excitation":[67],"(<150":[68],"nm).":[69],"It":[70],"thus":[72],"particularly":[73],"useful":[74],"studies":[76],"hormone":[79],"secretion.":[80],"A":[81,133],"brief":[82],"summary":[83],"this":[85],"approach":[86],"provided,":[88],"as":[89,91],"description":[93],"physical":[96],"basis":[97],"technique":[100],"tools":[103],"implement":[105],"TIRF":[106,136],"using":[107],"standard":[109],"microscope.":[111],"We":[112],"also":[113,147],"detail":[114],"different":[116],"fluorescent":[117],"probes":[118],"which":[119],"can":[120],"be":[121],"used":[122],"detect":[124],"how":[127],"analyze":[129],"data":[131],"obtained.":[132],"comparison":[134],"between":[135],"other":[138],"imaging":[139],"modalities":[140],"including":[141],"confocal":[142],"multiphoton":[144],"microscopy":[145],"included.":[148]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
