{"id":"https://openalex.org/W2054530395","doi":"https://doi.org/10.1002/jbio.200710024","title":"Fluorescent proteins for single\u2010molecule fluorescence applications","display_name":"Fluorescent proteins for single\u2010molecule fluorescence applications","publication_year":2008,"publication_date":"2008-02-05","ids":{"openalex":"https://openalex.org/W2054530395","doi":"https://doi.org/10.1002/jbio.200710024","mag":"2054530395","pmid":"https://pubmed.ncbi.nlm.nih.gov/19343637"},"language":"en","primary_location":{"id":"doi:10.1002/jbio.200710024","doi":"https://doi.org/10.1002/jbio.200710024","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jbio.200710024","pdf_url":null,"source":{"id":"https://openalex.org/S47864364","display_name":"Journal of Biophotonics","issn_l":"1864-063X","issn":["1864-063X","1864-0648"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biophotonics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040432343","display_name":"Britta Seefeldt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20121455","display_name":"Bielefeld University","ror":"https://ror.org/02hpadn98","country_code":"DE","type":"education","lineage":["https://openalex.org/I20121455"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Britta Seefeldt","raw_affiliation_strings":["Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052555319","display_name":"Robert T. Kasper","orcid":"https://orcid.org/0000-0002-3457-1931","observed_orcids":["https://orcid.org/0000-0002-3457-1931"]},"institutions":[{"id":"https://openalex.org/I20121455","display_name":"Bielefeld University","ror":"https://ror.org/02hpadn98","country_code":"DE","type":"education","lineage":["https://openalex.org/I20121455"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Robert Kasper","raw_affiliation_strings":["Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universita\u0308tsstrasse 25, 33615 Bielefeld, Germany","Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universita\u0308tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]},{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079856412","display_name":"Thorsten Seidel","orcid":"https://orcid.org/0000-0003-4423-3001","observed_orcids":["https://orcid.org/0000-0003-4423-3001"]},"institutions":[{"id":"https://openalex.org/I20121455","display_name":"Bielefeld University","ror":"https://ror.org/02hpadn98","country_code":"DE","type":"education","lineage":["https://openalex.org/I20121455"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Thorsten Seidel","raw_affiliation_strings":["Department of Biochemistry and Physiology of Plants, University of Bielefeld, 33501 Bielefeld, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Physiology of Plants, University of Bielefeld, 33501 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048312093","display_name":"Philip Tinnefeld","orcid":"https://orcid.org/0000-0003-4290-7770","observed_orcids":["https://orcid.org/0000-0003-4290-7770"]},"institutions":[{"id":"https://openalex.org/I2801304276","display_name":"Center for NanoScience","ror":"https://ror.org/002epp671","country_code":"DE","type":"facility","lineage":["https://openalex.org/I2801304276","https://openalex.org/I8204097"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Philip Tinnefeld","raw_affiliation_strings":["Applied Physics, Department of Applied Physics \u2013 Biophysics, and Center for Nanoscience, Ludwig-Maximilians Universita\u0308t Mu\u0308nchen, Amalienstrasse 54, 80799 Mu\u0308nchen, Germany","Applied Physics, Department of Applied Physics \u2013 Biophysics, and Center for Nanoscience, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, Amalienstrasse 54, 80799 M\u00fcnchen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Physics, Department of Applied Physics \u2013 Biophysics, and Center for Nanoscience, Ludwig-Maximilians Universita\u0308t Mu\u0308nchen, Amalienstrasse 54, 80799 Mu\u0308nchen, Germany","institution_ids":["https://openalex.org/I2801304276","https://openalex.org/I8204097"]},{"raw_affiliation_string":"Applied Physics, Department of Applied Physics \u2013 Biophysics, and Center for Nanoscience, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, Amalienstrasse 54, 80799 M\u00fcnchen, Germany","institution_ids":["https://openalex.org/I2801304276","https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012666877","display_name":"Karl\u2010Josef Dietz","orcid":"https://orcid.org/0000-0003-0311-2182","observed_orcids":["https://orcid.org/0000-0003-0311-2182"]},"institutions":[{"id":"https://openalex.org/I20121455","display_name":"Bielefeld University","ror":"https://ror.org/02hpadn98","country_code":"DE","type":"education","lineage":["https://openalex.org/I20121455"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Karl\u2010Josef Dietz","raw_affiliation_strings":["Department of Biochemistry and Physiology of Plants, University of Bielefeld, 33501 Bielefeld, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Physiology of Plants, University of Bielefeld, 33501 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032450795","display_name":"Mike Heilemann","orcid":"https://orcid.org/0000-0002-9821-3578","observed_orcids":["https://orcid.org/0000-0002-9821-3578"]},"institutions":[{"id":"https://openalex.org/I20121455","display_name":"Bielefeld University","ror":"https://ror.org/02hpadn98","country_code":"DE","type":"education","lineage":["https://openalex.org/I20121455"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Mike Heilemann","raw_affiliation_strings":["Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universita\u0308tsstrasse 25, 33615 Bielefeld, Germany","Markus Sauer, Phone: +49 521 106 5450","Mike Heilemann, Phone: +49 521 106 5442","Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universita\u0308tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]},{"raw_affiliation_string":"Markus Sauer, Phone: +49 521 106 5450","institution_ids":[]},{"raw_affiliation_string":"Mike Heilemann, Phone: +49 521 106 5442","institution_ids":[]},{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006887252","display_name":"Markus Sauer","orcid":"https://orcid.org/0000-0002-1692-3219","observed_orcids":["https://orcid.org/0000-0002-1692-3219"]},"institutions":[{"id":"https://openalex.org/I20121455","display_name":"Bielefeld University","ror":"https://ror.org/02hpadn98","country_code":"DE","type":"education","lineage":["https://openalex.org/I20121455"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Markus Sauer","raw_affiliation_strings":["Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universita\u0308tsstrasse 25, 33615 Bielefeld, Germany","Markus Sauer, Phone: +49 521 106 5450","Mike Heilemann, Phone: +49 521 106 5442","Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universita\u0308tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]},{"raw_affiliation_string":"Markus Sauer, Phone: +49 521 106 5450","institution_ids":[]},{"raw_affiliation_string":"Mike Heilemann, Phone: +49 521 106 5442","institution_ids":[]},{"raw_affiliation_string":"Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universit\u00e4tsstrasse 25, 33615 Bielefeld, Germany","institution_ids":["https://openalex.org/I20121455"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5006887252","https://openalex.org/A5032450795"],"corresponding_institution_ids":["https://openalex.org/I20121455"],"apc_list":{"value":3370,"currency":"USD","value_usd":3370},"apc_paid":null,"fwci":2.9148,"has_fulltext":false,"cited_by_count":72,"citation_normalized_percentile":{"value":0.9109556,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"1","issue":"1","first_page":"74","last_page":"82"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11240","display_name":"Luminescence and Fluorescent Materials","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11129","display_name":"Photochemistry and Electron Transfer Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescent-proteins","display_name":"fluorescent proteins","score":0.9559999704360962},{"id":"https://openalex.org/keywords/single-molecule-fluorescence","display_name":"single-molecule fluorescence","score":0.8709999918937683},{"id":"https://openalex.org/keywords/mcherry","display_name":"mCherry","score":0.9929999709129333},{"id":"https://openalex.org/keywords/dsred","display_name":"DsRed","score":0.9860000014305115},{"id":"https://openalex.org/keywords/green-fluorescent-protein","display_name":"green fluorescent protein","score":0.722000002861023},{"id":"https://openalex.org/keywords/yellow-fluorescent-protein","display_name":"YFP","score":0.9589999914169312},{"id":"https://openalex.org/keywords/photostability","display_name":"photostability","score":0.9769999980926514},{"id":"https://openalex.org/keywords/fluorescence-fluctuations","display_name":"fluorescence intensity fluctuations","score":0.7820000052452087},{"id":"https://openalex.org/keywords/fluorescence-quantum-yield","display_name":"fluorescence quantum yield","score":0.8899999856948853},{"id":"https://openalex.org/keywords/fluorescence-lifetime","display_name":"fluorescence lifetime","score":0.984000027179718},{"id":"https://openalex.org/keywords/fluorescence-resonance-energy-transfer","display_name":"fluorescence resonance energy transfer","score":0.8399999737739563},{"id":"https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy","display_name":"fluorescence lifetime imaging","score":0.9649999737739563},{"id":"https://openalex.org/keywords/multicolor-labeling","display_name":"multicolor labeling","score":0.8140000104904175}],"concepts":[{"id":"https://openalex.org/C2779260011","wikidata":"https://www.wikidata.org/wiki/Q412287","display_name":"mCherry","level":4,"score":0.9761072397232056},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.9112635254859924},{"id":"https://openalex.org/C62535639","wikidata":"https://www.wikidata.org/wiki/Q15055422","display_name":"Fluorescence in the life sciences","level":4,"score":0.9098557233810425},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.9067889451980591},{"id":"https://openalex.org/C2778617687","wikidata":"https://www.wikidata.org/wiki/Q2448456","display_name":"Fluorophore","level":3,"score":0.8517103791236877},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.6767638921737671},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5754792094230652},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5586963891983032},{"id":"https://openalex.org/C123243835","wikidata":"https://www.wikidata.org/wiki/Q863465","display_name":"Bimolecular fluorescence complementation","level":3,"score":0.5321767926216125},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5317137837409973},{"id":"https://openalex.org/C199191878","wikidata":"https://www.wikidata.org/wiki/Q6504739","display_name":"Single-molecule experiment","level":3,"score":0.5050130486488342},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.49010738730430603},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.48578742146492004},{"id":"https://openalex.org/C49853544","wikidata":"https://www.wikidata.org/wiki/Q206229","display_name":"Biomolecule","level":2,"score":0.47824564576148987},{"id":"https://openalex.org/C171001562","wikidata":"https://www.wikidata.org/wiki/Q2856048","display_name":"Quantum yield","level":3,"score":0.44367244839668274},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13216719031333923},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1257725954055786},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.08703038096427917},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.06979817152023315},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D031541","descriptor_name":"Fluorescence Resonance Energy Transfer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1002/jbio.200710024","doi":"https://doi.org/10.1002/jbio.200710024","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jbio.200710024","pdf_url":null,"source":{"id":"https://openalex.org/S47864364","display_name":"Journal of Biophotonics","issn_l":"1864-063X","issn":["1864-063X","1864-0648"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biophotonics","raw_type":"journal-article"},{"id":"pmid:19343637","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19343637","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biophotonics","raw_type":"Journal Article"},{"id":"pmh:oai:pub.librecat.org:1636544","doi":null,"is_oa":false,"landing_page_url":"https://pub.uni-bielefeld.de/record/1636544","pdf_url":null,"source":{"id":"https://openalex.org/S4306401671","display_name":"PUB \u2013 Publications at Bielefeld University (Bielefeld University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I20121455","host_organization_name":"Bielefeld University","host_organization_lineage":["https://openalex.org/I20121455"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Seefeldt B, Kasper R, Seidel T, et al. Fluorescent proteins for single-molecule fluorescence applications. &lt;em&gt;Journal of Biophotonics&lt;/em&gt;. 2008;1(1):74-82.","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:pub.uni-bielefeld.de:1636544","doi":null,"is_oa":false,"landing_page_url":"https://pub.uni-bielefeld.de/record/1636544","pdf_url":null,"source":{"id":"https://openalex.org/S4306401624","display_name":"Publikationen an der Universit\u00e4t Bielefeld (Universit\u00e4t Bielefeld)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I20121455","host_organization_name":"Bielefeld University","host_organization_lineage":["https://openalex.org/I20121455"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Seefeldt B, Kasper R, Seidel T, et al. Fluorescent proteins for single-molecule fluorescence applications. &lt;em&gt;Journal of Biophotonics&lt;/em&gt;. 2008;1(1):74-82.","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1544900705","https://openalex.org/W1626513324","https://openalex.org/W1657499324","https://openalex.org/W1969103349","https://openalex.org/W1973244829","https://openalex.org/W1973306521","https://openalex.org/W1974642741","https://openalex.org/W1978176055","https://openalex.org/W1983188523","https://openalex.org/W1988326309","https://openalex.org/W1998337135","https://openalex.org/W1998948607","https://openalex.org/W1999046814","https://openalex.org/W1999056422","https://openalex.org/W1999531802","https://openalex.org/W2027480851","https://openalex.org/W2039712591","https://openalex.org/W2043450412","https://openalex.org/W2045006795","https://openalex.org/W2052512546","https://openalex.org/W2054455698","https://openalex.org/W2054665524","https://openalex.org/W2055100785","https://openalex.org/W2056963450","https://openalex.org/W2088864569","https://openalex.org/W2090307809","https://openalex.org/W2099906733","https://openalex.org/W2106060961","https://openalex.org/W2115734610","https://openalex.org/W2118065916","https://openalex.org/W2123847884","https://openalex.org/W2127068865","https://openalex.org/W2164565944","https://openalex.org/W2169791141"],"related_works":["https://openalex.org/W1999531802","https://openalex.org/W2024396937","https://openalex.org/W2100389936","https://openalex.org/W2001522957","https://openalex.org/W1965411905","https://openalex.org/W2019624766","https://openalex.org/W4302164647","https://openalex.org/W2049700149","https://openalex.org/W1987534489","https://openalex.org/W2077803788"],"abstract_inverted_index":{"We":[0],"present":[1],"single-molecule":[2,20,31,65],"fluorescence":[3,21,46,66,75,99,104],"data":[4],"of":[5,16,77,93],"fluorescent":[6,51,84],"proteins":[7,52,85],"GFP,":[8],"YFP,":[9],"DsRed,":[10],"and":[11,19,37,73,86,91],"mCherry,":[12],"a":[13],"new":[14],"derivative":[15],"DsRed.":[17],"Ensemble":[18],"experiments":[22],"proved":[23],"mCherry":[24,42,59,80],"as":[25,60],"an":[26,61],"ideally":[27],"suited":[28],"fluorophore":[29],"for":[30,89],"applications,":[32],"demonstrated":[33],"by":[34],"high":[35,97],"photostability":[36],"rare":[38],"fluorescence-intensity":[39],"fluctuations.":[40],"Although":[41],"exhibits":[43],"the":[44,50],"lowest":[45],"quantum":[47],"yield":[48],"among":[49],"investigated,":[53],"its":[54,70],"superior":[55],"photophysical":[56],"characteristics":[57,72],"suggest":[58],"ideal":[62],"alternative":[63],"in":[64],"experiments.":[67],"Due":[68],"to":[69],"spectral":[71],"short":[74],"lifetime":[76,105],"1.46":[78],"ns,":[79],"complements":[81],"other":[82],"existing":[83],"is":[87],"recommended":[88],"tracking":[90],"localization":[92],"target":[94],"molecules":[95],"with":[96],"accuracy,":[98],"resonance":[100],"energy":[101],"transfer":[102],"(FRET),":[103],"imaging":[106],"microscopy":[107],"(FLIM),":[108],"or":[109],"multicolor":[110],"applications.":[111]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":8},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}
