{"id":"https://openalex.org/W2053613399","doi":"https://doi.org/10.1083/jcb.80.3.751","title":"Isolation of concanavalin a caps during various stages of formation and their association with actin and myosin","display_name":"Isolation of concanavalin a caps during various stages of formation and their association with actin and myosin","publication_year":1979,"publication_date":"1979-03-01","ids":{"openalex":"https://openalex.org/W2053613399","doi":"https://doi.org/10.1083/jcb.80.3.751","mag":"2053613399","pmid":"https://pubmed.ncbi.nlm.nih.gov/457767","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2110358"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.80.3.751","doi":"https://doi.org/10.1083/jcb.80.3.751","is_oa":true,"landing_page_url":"https://doi.org/10.1083/jcb.80.3.751","pdf_url":"https://rupress.org/jcb/article-pdf/80/3/751/1073509/751.pdf","source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Cell Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://rupress.org/jcb/article-pdf/80/3/751/1073509/751.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014100567","display_name":"John S. Condeelis","orcid":"https://orcid.org/0000-0001-8826-2336","observed_orcids":["https://orcid.org/0000-0001-8826-2336"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"J Condeelis","raw_affiliation_strings":["Anatomy & Structural Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Anatomy & Structural Biology","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5014100567"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":8.864,"has_fulltext":true,"cited_by_count":163,"citation_normalized_percentile":{"value":0.98330879,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"80","issue":"3","first_page":"751","last_page":"758"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.382099986076355,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.382099986076355,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.35690000653266907,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.12999999523162842,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/concanavalin-a","display_name":"concanavalin A","score":0.8560000061988831},{"id":"https://openalex.org/keywords/dictyostelium-discoideum","display_name":"Dictyostelium discoideum","score":0.9169999957084656},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.6060000061988831},{"id":"https://openalex.org/keywords/actin","display_name":"actin","score":0.8289999961853027},{"id":"https://openalex.org/keywords/myosin","display_name":"myosin","score":1.0},{"id":"https://openalex.org/keywords/actin-filaments","display_name":"F-actin","score":0.6320000290870667},{"id":"https://openalex.org/keywords/myosin-filaments","display_name":"myosin filaments","score":0.921999990940094}],"concepts":[{"id":"https://openalex.org/C6997183","wikidata":"https://www.wikidata.org/wiki/Q271727","display_name":"Myosin","level":2,"score":0.7830591797828674},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6985033750534058},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.6323468685150146},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.6171292066574097},{"id":"https://openalex.org/C19869320","wikidata":"https://www.wikidata.org/wiki/Q419277","display_name":"Concanavalin A","level":3,"score":0.5664684772491455},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.54152911901474},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.5252801179885864},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.5133079886436462},{"id":"https://openalex.org/C3620293","wikidata":"https://www.wikidata.org/wiki/Q14908027","display_name":"Actin remodeling","level":5,"score":0.5118924379348755},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.47359374165534973},{"id":"https://openalex.org/C2775969001","wikidata":"https://www.wikidata.org/wiki/Q134351","display_name":"Dictyostelium discoideum","level":3,"score":0.43955469131469727},{"id":"https://openalex.org/C197462201","wikidata":"https://www.wikidata.org/wiki/Q2476074","display_name":"Cell surface receptor","level":3,"score":0.4354550838470459},{"id":"https://openalex.org/C189040839","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Microfilament","level":4,"score":0.4134944975376129},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3752630054950714},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36679285764694214},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.35412371158599854},{"id":"https://openalex.org/C2993400109","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Actin cytoskeleton","level":4,"score":0.3154280483722687},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D003208","descriptor_name":"Concanavalin A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004023","descriptor_name":"Dictyostelium","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004023","descriptor_name":"Dictyostelium","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D007152","descriptor_name":"Immunologic Capping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009218","descriptor_name":"Myosins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D009218","descriptor_name":"Myosins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011952","descriptor_name":"Receptors, Concanavalin A","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011952","descriptor_name":"Receptors, Concanavalin A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011955","descriptor_name":"Receptors, Drug","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1083/jcb.80.3.751","doi":"https://doi.org/10.1083/jcb.80.3.751","is_oa":true,"landing_page_url":"https://doi.org/10.1083/jcb.80.3.751","pdf_url":"https://rupress.org/jcb/article-pdf/80/3/751/1073509/751.pdf","source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Cell Biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2110358","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2110358","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmid:457767","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/457767","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.280.4436","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4436","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/56/91/J_Cell_Biol_1979_Mar_1_80(3)_751-758.tar.gz","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1083/jcb.80.3.751","doi":"https://doi.org/10.1083/jcb.80.3.751","is_oa":true,"landing_page_url":"https://doi.org/10.1083/jcb.80.3.751","pdf_url":"https://rupress.org/jcb/article-pdf/80/3/751/1073509/751.pdf","source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Cell Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2053613399.pdf","grobid_xml":"https://content.openalex.org/works/W2053613399.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W1497478067","https://openalex.org/W1553626628","https://openalex.org/W1970092792","https://openalex.org/W1970101963","https://openalex.org/W1970266617","https://openalex.org/W1994255416","https://openalex.org/W1998938420","https://openalex.org/W1998950111","https://openalex.org/W2006201918","https://openalex.org/W2007524790","https://openalex.org/W2048683363","https://openalex.org/W2135630126","https://openalex.org/W2160971658","https://openalex.org/W2396894690","https://openalex.org/W2410530349","https://openalex.org/W2461696474"],"related_works":["https://openalex.org/W2048683363","https://openalex.org/W2409085313","https://openalex.org/W2015367719","https://openalex.org/W7100891996","https://openalex.org/W2086638372","https://openalex.org/W2014953060","https://openalex.org/W2072663410","https://openalex.org/W14461437","https://openalex.org/W2104254394","https://openalex.org/W2099842635"],"abstract_inverted_index":{"Regions":[0],"of":[1,4,29,47,57,67,113,142,147,171,181,191,220,231],"plasma":[2,76,200],"membrane":[3,32,77,201],"dictyostelium":[5],"discoideum":[6],"amoebae":[7],"that":[8,88,117,151,164,195,216],"contain":[9],"concanavalin":[10],"A":[11,59,116],"(Con":[12],"A)-receptor":[13],"complexes":[14,177,234],"are":[15,105,137,165],"more":[16,81],"resistant":[17],"to":[18,39,60,84,120,167,198],"disruption":[19],"by":[20,94],"Triton":[21],"X-100.":[22],"This":[23],"resistance":[24],"makes":[25],"possible":[26],"the":[27,41,51,61,68,75,85,114,121,145,154,158,168,172,185,189,199,205,209,217,229,239],"isolation":[28],"Con":[30,58,115,210,232],"A-associated":[31],"fragments":[33],"in":[34,129],"sufficient":[35],"quantity":[36],"and":[37,44,71,90,99,149,161,193,223],"homogeneity":[38],"permit":[40],"direct":[42],"biochemical":[43],"ultrastructural":[45],"study":[46],"receptor-cytoskeletal":[48],"interactions":[49],"across":[50],"cell":[52,62,122,240],"membrane.":[53],"After":[54],"specific":[55],"binding":[56],"surface,":[63],"a":[64,127,130,236],"large":[65],"amount":[66,146,190],"cell's":[69],"actin":[70,89,148,192,222],"myosin":[72,150,194,224],"copurifies":[73,152],"with":[74,97,153,208],"fragments.":[78],"Myosin":[79],"is":[80,91,118,124,187,202,226],"loosely":[82],"bound":[83,119,197],"isolated":[86,155,173],"membranes":[87,96,156,174],"efficiently":[92],"removed":[93],"treating":[95],"ATP":[98],"low":[100],"ionic":[101],"strength.":[102],"If":[103],"cells":[104,136],"not":[106],"lysed":[107,138],"immediately":[108],"after":[109],"lectin":[110],"binding,":[111],"all":[112,179],"surface":[123,170],"swept":[125],"into":[126,204,235],"cap":[128,143,182,186,206,237],"process":[131],"requiring":[132],"metabolic":[133],"energy.":[134],"When":[135],"at":[139],"different":[140],"stages":[141,180],"formation,":[144],"remains":[157],"same.":[159],"Thick":[160],"thin":[162],"filaments":[163,225],"attached":[166],"protoplasmic":[169],"underlie":[175],"lectin-receptor":[176],"during":[178],"formation.":[183],"Once":[184],"complete,":[188],"tightly":[196],"concentrated":[203],"along":[207],"A-receptor":[211,233],"complexes.":[212],"These":[213],"results":[214],"suggest":[215],"ATP-dependent":[218],"sliding":[219],"membrane-associated":[221],"responsible":[227],"for":[228],"accumulation":[230],"on":[238],"surface.":[241]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}