{"id":"https://openalex.org/W2053166995","doi":"https://doi.org/10.3136/fstr.6.115","title":"A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria.","display_name":"A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria.","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W2053166995","doi":"https://doi.org/10.3136/fstr.6.115","mag":"2053166995"},"language":"en","primary_location":{"id":"doi:10.3136/fstr.6.115","doi":"https://doi.org/10.3136/fstr.6.115","is_oa":true,"landing_page_url":"https://doi.org/10.3136/fstr.6.115","pdf_url":"https://www.jstage.jst.go.jp/article/fstr/6/2/6_2_115/_pdf","source":{"id":"https://openalex.org/S60340032","display_name":"Food Science and Technology Research","issn_l":"1344-6606","issn":["1344-6606","1881-3984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Food Science and Technology Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jstage.jst.go.jp/article/fstr/6/2/6_2_115/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055908604","display_name":"Koji Nagashima","orcid":"https://orcid.org/0000-0003-4933-3239","observed_orcids":["https://orcid.org/0000-0003-4933-3239"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Koji NAGASHIMA","raw_affiliation_strings":["Hokkaido Food Processing Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Food Processing Research Center","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089998559","display_name":"Takeshi Shimizu","orcid":"https://orcid.org/0000-0002-1344-7210","observed_orcids":["https://orcid.org/0000-0002-1344-7210"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Takeshi SHIMIZU","raw_affiliation_strings":["Hokkaido Food Processing Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Food Processing Research Center","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110029108","display_name":"Kouichi Takeshi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210158475","display_name":"Hokkaido Institute of Public Health","ror":"https://ror.org/050hwd209","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210158475"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kouichi TAKESHI","raw_affiliation_strings":["Hokkaido Institute of Public Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Institute of Public Health","institution_ids":["https://openalex.org/I4210158475"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084898660","display_name":"Makoto Kawakami","orcid":"https://orcid.org/0000-0003-0639-0882","observed_orcids":["https://orcid.org/0000-0003-0639-0882"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Makoto KAWAKAMI","raw_affiliation_strings":["Hokkaido Food Processing Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Food Processing Research Center","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040302379","display_name":"Daisuke Yasokawa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Daisuke YASOKAWA","raw_affiliation_strings":["Hokkaido Food Processing Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Food Processing Research Center","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057797198","display_name":"Ryoji Nakagawa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ryoji NAKAGAWA","raw_affiliation_strings":["Hokkaido Food Processing Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Food Processing Research Center","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110827643","display_name":"Yukihiro OKUMURA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yukihiro OKUMURA","raw_affiliation_strings":["Hokkaido Food Processing Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido Food Processing Research Center","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1719,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":{"value":0.48196248,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"6","issue":"2","first_page":"115","last_page":"118"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.7074999809265137,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.7074999809265137,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.06750000268220901,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12388","display_name":"Identification and Quantification in Food","score":0.036400001496076584,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacterial-detection","display_name":"bacterial detection","score":0.7229999899864197},{"id":"https://openalex.org/keywords/gram-positive-bacteria","display_name":"Gram-positive bacteria","score":0.7720000147819519},{"id":"https://openalex.org/keywords/chelex-100","display_name":"Chelex-100","score":0.9440000057220459},{"id":"https://openalex.org/keywords/16s-rrna-gene","display_name":"16S rRNA gene","score":0.9179999828338623},{"id":"https://openalex.org/keywords/universal-primers","display_name":"universal primers","score":0.8820000290870667},{"id":"https://openalex.org/keywords/food-safety","display_name":"food safety","score":0.3709999918937683},{"id":"https://openalex.org/keywords/pcr-template-preparation","display_name":"PCR template preparation","score":0.5180000066757202}],"concepts":[{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.7609140872955322},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.6925487518310547},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.6783257126808167},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5702972412109375},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5403518676757812},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4794410765171051},{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.4160791337490082},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3940345048904419},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.38215136528015137},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3547266125679016},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21072125434875488}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3136/fstr.6.115","doi":"https://doi.org/10.3136/fstr.6.115","is_oa":true,"landing_page_url":"https://doi.org/10.3136/fstr.6.115","pdf_url":"https://www.jstage.jst.go.jp/article/fstr/6/2/6_2_115/_pdf","source":{"id":"https://openalex.org/S60340032","display_name":"Food Science and Technology Research","issn_l":"1344-6606","issn":["1344-6606","1881-3984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Food Science and Technology Research","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.3136/fstr.6.115","doi":"https://doi.org/10.3136/fstr.6.115","is_oa":true,"landing_page_url":"https://doi.org/10.3136/fstr.6.115","pdf_url":"https://www.jstage.jst.go.jp/article/fstr/6/2/6_2_115/_pdf","source":{"id":"https://openalex.org/S60340032","display_name":"Food Science and Technology Research","issn_l":"1344-6606","issn":["1344-6606","1881-3984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Food Science and Technology Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2053166995.pdf","grobid_xml":"https://content.openalex.org/works/W2053166995.grobid-xml"},"referenced_works_count":14,"referenced_works":["https://openalex.org/W1958790533","https://openalex.org/W1962223937","https://openalex.org/W1978065201","https://openalex.org/W2053954611","https://openalex.org/W2069956534","https://openalex.org/W2088002866","https://openalex.org/W2091309425","https://openalex.org/W2102767606","https://openalex.org/W2103930732","https://openalex.org/W2114136095","https://openalex.org/W2156380818","https://openalex.org/W2160044108","https://openalex.org/W4212864994","https://openalex.org/W4240489031"],"related_works":["https://openalex.org/W1994684228","https://openalex.org/W1993707017","https://openalex.org/W2082622581","https://openalex.org/W4409376082","https://openalex.org/W2030972523","https://openalex.org/W2073014331","https://openalex.org/W2624949466","https://openalex.org/W1960606583","https://openalex.org/W2327208813","https://openalex.org/W1947175631"],"abstract_inverted_index":{"We":[0],"devised":[1],"a":[2,17],"simple":[3],"and":[4],"efficient":[5],"method":[6],"of":[7,23,50,53],"preparing":[8],"chromosomal":[9],"DNA":[10,33],"from":[11],"Gram-positive":[12],"bacteria":[13,55],"for":[14,69],"use":[15],"as":[16,43,45],"template":[18,32],"in":[19],"PCR,":[20],"which":[21],"consists":[22],"achromopeptidase":[24],"treatment":[25],"followed":[26],"by":[27,35],"Chelex":[28],"100":[29],"treatment.":[30],"Using":[31],"prepared":[34],"this":[36],"method,":[37],"it":[38],"was":[39],"possible":[40],"to":[41],"detect":[42],"few":[44],"one":[46],"colony":[47],"forming":[48],"unit":[49],"some":[51],"kinds":[52],"food-related":[54],"using":[56],"universal":[57],"primers":[58,68],"targeting":[59],"the":[60],"bacterial":[61,70],"16S":[62],"ribosomal":[63],"RNA":[64],"gene":[65],"or":[66],"specific":[67],"genes":[71],"under":[72],"fixed":[73],"thermocycling":[74],"conditions.":[75]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-08-11T07:18:39.950985","created_date":"2016-06-24T00:00:00"}