{"id":"https://openalex.org/W2053065416","doi":"https://doi.org/10.1021/bi00307a011","title":"Dynamic equilibrium between the two conformational states of spin-labeled tropomyosin","display_name":"Dynamic equilibrium between the two conformational states of spin-labeled tropomyosin","publication_year":1984,"publication_date":"1984-06-01","ids":{"openalex":"https://openalex.org/W2053065416","doi":"https://doi.org/10.1021/bi00307a011","mag":"2053065416","pmid":"https://pubmed.ncbi.nlm.nih.gov/6087878"},"language":"en","primary_location":{"id":"doi:10.1021/bi00307a011","doi":"https://doi.org/10.1021/bi00307a011","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00307a011","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057469369","display_name":"Philip Graceffa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Philip Graceffa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5064474716","display_name":"Sherwin S. Lehrer","orcid":"https://orcid.org/0000-0002-0284-4472","observed_orcids":["https://orcid.org/0000-0002-0284-4472"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sherwin S. Lehrer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":3.521,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.92927794,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"12","first_page":"2606","last_page":"2612"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12381","display_name":"Electron Spin Resonance Studies","score":0.9634000062942505,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12381","display_name":"Electron Spin Resonance Studies","score":0.9634000062942505,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.03449999913573265,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10612","display_name":"Magnetism in coordination complexes","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2504","display_name":"Electronic, Optical and Magnetic Materials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tropomyosin","display_name":"tropomyosin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/spin-labeling","display_name":"spin labeling","score":0.8259999752044678},{"id":"https://openalex.org/keywords/conformational-equilibrium","display_name":"conformational equilibrium","score":0.8149999976158142},{"id":"https://openalex.org/keywords/thermal-denaturation","display_name":"thermal denaturation","score":0.796999990940094},{"id":"https://openalex.org/keywords/partially-unfolded-intermediate","display_name":"partially unfolded intermediate","score":0.699999988079071},{"id":"https://openalex.org/keywords/conformational-fluctuations","display_name":"conformational fluctuations","score":0.7269999980926514},{"id":"https://openalex.org/keywords/spectral-subtraction","display_name":"spectral subtraction","score":0.5289999842643738},{"id":"https://openalex.org/keywords/van-t-hoff-analysis","display_name":"van't Hoff analysis","score":0.5709999799728394},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.39500001072883606}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.7444138526916504},{"id":"https://openalex.org/C2778447006","wikidata":"https://www.wikidata.org/wiki/Q1156474","display_name":"Icon","level":2,"score":0.6511670351028442},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5516579151153564},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.4772060811519623},{"id":"https://openalex.org/C205875245","wikidata":"https://www.wikidata.org/wiki/Q418454","display_name":"Tropomyosin","level":3,"score":0.4253532290458679},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.36990636587142944},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.2952221930027008},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.24679476022720337},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.12289822101593018},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.08192715048789978},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C6997183","wikidata":"https://www.wikidata.org/wiki/Q271727","display_name":"Myosin","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004578","descriptor_name":"Electron Spin Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009132","descriptor_name":"Muscles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013113","descriptor_name":"Spin Labels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014335","descriptor_name":"Tropomyosin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00307a011","doi":"https://doi.org/10.1021/bi00307a011","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00307a011","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:6087878","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6087878","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W29716068","https://openalex.org/W909996232","https://openalex.org/W1486373758","https://openalex.org/W1495596945","https://openalex.org/W1546253470","https://openalex.org/W1556587164","https://openalex.org/W1575474218","https://openalex.org/W1620274191","https://openalex.org/W1890150156","https://openalex.org/W1968036996","https://openalex.org/W1969047020","https://openalex.org/W1973887565","https://openalex.org/W1976649968","https://openalex.org/W1980839154","https://openalex.org/W1982112682","https://openalex.org/W1986049053","https://openalex.org/W1995903700","https://openalex.org/W2001715586","https://openalex.org/W2002906547","https://openalex.org/W2006370393","https://openalex.org/W2008253062","https://openalex.org/W2008463384","https://openalex.org/W2009354400","https://openalex.org/W2013215295","https://openalex.org/W2017907072","https://openalex.org/W2018076547","https://openalex.org/W2038185605","https://openalex.org/W2050441430","https://openalex.org/W2064789937","https://openalex.org/W2092247667","https://openalex.org/W2092746485","https://openalex.org/W2135157170","https://openalex.org/W2161153356","https://openalex.org/W2177330293","https://openalex.org/W2198115699","https://openalex.org/W2400999883","https://openalex.org/W2408586011","https://openalex.org/W2409800784"],"related_works":["https://openalex.org/W2460629143","https://openalex.org/W2268012538","https://openalex.org/W2038185605","https://openalex.org/W1930009513","https://openalex.org/W1486373758","https://openalex.org/W2086926971","https://openalex.org/W2075642393","https://openalex.org/W2098176418","https://openalex.org/W1997110614","https://openalex.org/W2468776506"],"abstract_inverted_index":{"Tropomyosin":[0],"was":[1,16,129,148,158,205],"labeled":[2],"with":[3,71,83,96,106,174,223,230],"a":[4,12,30,76,107],"maleimide":[5],"nitroxide":[6],"spin-label":[7,229],"attached":[8],"to":[9,131,220],"cysteine-190":[10,112,254],"via":[11],"succinimido":[13,226,233],"ring":[14,234],"which":[15,42,56,115,143],"subsequently":[17],"opened":[18,225],"by":[19],"incubation":[20],"at":[21,51,63,150,191],"alkaline":[22],"pH.":[23],"Electron":[24],"spin":[25],"resonance":[26],"(ESR)":[27],"spectra":[28],"showed":[29],"temperature-dependent":[31],"equilibrium,":[32],"below":[33],"the":[34,79,84,97,102,111,120,133,162,202,208,213,221,224,228,239,244,248,253],"main":[35],"unfolding":[36,86,122],"transition":[37],"of":[38,78,88,123,136,198,212],"tropomyosin,":[39],"between":[40,169],"labels":[41,93,105,141],"were":[43,57,69,94],"restricted":[44],"in":[45,119,166,243,252],"their":[46],"motion":[47],"(strongly":[48],"immobilized),":[49,61],"predominating":[50,62],"low":[52],"temperatures,":[53],"and":[54,101,138,187,210],"those":[55],"highly":[58],"mobile":[59],"(weakly":[60],"higher":[64],"temperatures.":[65,152],"These":[66],"label":[67,222],"states":[68,74,173],"associated":[70,95],"two":[72,171,214],"protein":[73,164],"from":[75,142,207],"comparison":[77],"ESR":[80,215],"spectral":[81,126,216],"changes":[82],"thermal":[85,121,167],"profile":[87],"tropomyosin.":[89,124],"The":[90],"strongly":[91,137,236],"immobilized":[92,104,140,237],"completely":[98],"folded":[99],"molded":[100],"weakly":[103,139],"partially":[108,245],"unfolded":[109,246],"(in":[110],"region)":[113],"state":[114,247],"is":[116,165],"an":[117,144,231],"intermediate":[118],"A":[125,153],"subtraction":[127],"technique":[128],"used":[130],"measure":[132],"concentration":[134],"ratio":[135],"equilibrium":[145,168],"constant,":[146],"K,":[147],"determined":[149],"different":[151],"linear":[154],"van't":[155],"Hoff":[156],"plot":[157],"obtained,":[159],"indicating":[160,241],"that":[161,242],"spin-labeled":[163],"these":[170],"conformational":[172,203],"delta":[175,180],"H":[176],"=":[177,182,189],"17":[178],"kcal/mol,":[179],"S":[181],"56":[183],"cal/(deg":[184],"X":[185],"mol),":[186],"K":[188],"1.0":[190],"34":[192],"degrees":[193],"C.":[194],"An":[195],"upper":[196],"limit":[197],"10(7)":[199],"s-1":[200],"for":[201],"fluctuation":[204],"estimated":[206],"shapes":[209],"separation":[211],"components.":[217],"In":[218],"contrast":[219],"ring,":[227],"intact":[232],"remained":[235],"on":[238],"protein,":[240],"molecule":[249],"retains":[250],"structure":[251],"region.":[255]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}