{"id":"https://openalex.org/W2053040211","doi":"https://doi.org/10.1074/jbc.270.5.2000","title":"A Topological Analysis of Goldfish Kainate Receptors Predicts Three Transmembrane Segments","display_name":"A Topological Analysis of Goldfish Kainate Receptors Predicts Three Transmembrane Segments","publication_year":1995,"publication_date":"1995-02-01","ids":{"openalex":"https://openalex.org/W2053040211","doi":"https://doi.org/10.1074/jbc.270.5.2000","mag":"2053040211","pmid":"https://pubmed.ncbi.nlm.nih.gov/7836426"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.270.5.2000","doi":"https://doi.org/10.1074/jbc.270.5.2000","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.270.5.2000","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.270.5.2000","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081269981","display_name":"Z. Galen Wo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]},{"id":"https://openalex.org/I4210151139","display_name":"New York State College of Veterinary Medicine","ror":"https://ror.org/04r17kf39","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4210151139"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Z. Galen Wo","raw_affiliation_strings":["Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853","From the Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4210151139"]},{"raw_affiliation_string":"From the Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4210151139"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5057714108","display_name":"Robert E. Oswald","orcid":"https://orcid.org/0000-0003-3028-7922","observed_orcids":["https://orcid.org/0000-0003-3028-7922"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]},{"id":"https://openalex.org/I4210151139","display_name":"New York State College of Veterinary Medicine","ror":"https://ror.org/04r17kf39","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4210151139"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Robert E. Oswald","raw_affiliation_strings":["Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853","From the Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4210151139"]},{"raw_affiliation_string":"From the Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4210151139"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5057714108"],"corresponding_institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4210151139"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.4792,"has_fulltext":false,"cited_by_count":66,"citation_normalized_percentile":{"value":0.94277908,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"270","issue":"5","first_page":"2000","last_page":"2009"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.011099999770522118,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/kainate-receptors","display_name":"kainate receptors","score":0.9449999928474426},{"id":"https://openalex.org/keywords/ionotropic-glutamate-receptors","display_name":"ionotropic glutamate receptors","score":0.8360000252723694},{"id":"https://openalex.org/keywords/receptor-topology","display_name":"receptor topology","score":0.6880000233650208},{"id":"https://openalex.org/keywords/transmembrane-helices","display_name":"transmembrane helices","score":0.8759999871253967},{"id":"https://openalex.org/keywords/goldfish","display_name":"goldfish","score":0.7630000114440918},{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.8399999737739563},{"id":"https://openalex.org/keywords/extracellular-loop","display_name":"extracellular loop","score":0.5350000262260437},{"id":"https://openalex.org/keywords/site-specific-antibodies","display_name":"site-specific antibodies","score":0.6050000190734863},{"id":"https://openalex.org/keywords/deletion-mutants","display_name":"deletion mutants","score":0.5109999775886536},{"id":"https://openalex.org/keywords/structure-function-relationship","display_name":"structure-function relationship","score":0.6700000166893005}],"concepts":[{"id":"https://openalex.org/C189184402","wikidata":"https://www.wikidata.org/wiki/Q3454536","display_name":"Kainate receptor","level":5,"score":0.7562383413314819},{"id":"https://openalex.org/C184720557","wikidata":"https://www.wikidata.org/wiki/Q7825049","display_name":"Topology (electrical circuits)","level":2,"score":0.5248960256576538},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5059935450553894},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.49794983863830566},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3740830421447754},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.3519866466522217},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.32926681637763977},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.1391623318195343},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12794464826583862},{"id":"https://openalex.org/C61174792","wikidata":"https://www.wikidata.org/wiki/Q420510","display_name":"Glutamate receptor","level":3,"score":0.06986072659492493},{"id":"https://openalex.org/C160268369","wikidata":"https://www.wikidata.org/wiki/Q295251","display_name":"AMPA receptor","level":4,"score":0.05510708689689636}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D006054","descriptor_name":"Goldfish","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006596","descriptor_name":"Hexosaminidases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007158","descriptor_name":"Immunologic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018092","descriptor_name":"Kainic Acid Receptors","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D019286","descriptor_name":"Endopeptidase K","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.270.5.2000","doi":"https://doi.org/10.1074/jbc.270.5.2000","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.270.5.2000","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:7836426","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7836426","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Comparative Study"}],"best_oa_location":{"id":"doi:10.1074/jbc.270.5.2000","doi":"https://doi.org/10.1074/jbc.270.5.2000","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.270.5.2000","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2383587547","display_name":"Biophysical and Biochemical Pharmacology","funder_award_id":"T32GM008210","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":63,"referenced_works":["https://openalex.org/W120263029","https://openalex.org/W161114408","https://openalex.org/W171632912","https://openalex.org/W276882634","https://openalex.org/W1515431754","https://openalex.org/W1519445799","https://openalex.org/W1532205945","https://openalex.org/W1571379445","https://openalex.org/W1584455845","https://openalex.org/W1586225177","https://openalex.org/W1599420609","https://openalex.org/W1966548233","https://openalex.org/W1975304761","https://openalex.org/W1982028592","https://openalex.org/W1986130768","https://openalex.org/W1991740949","https://openalex.org/W1992414754","https://openalex.org/W1994322758","https://openalex.org/W2002360326","https://openalex.org/W2002995942","https://openalex.org/W2003349457","https://openalex.org/W2003861265","https://openalex.org/W2005913521","https://openalex.org/W2006406301","https://openalex.org/W2006695112","https://openalex.org/W2009297045","https://openalex.org/W2017498808","https://openalex.org/W2019831795","https://openalex.org/W2021184142","https://openalex.org/W2023661816","https://openalex.org/W2024358879","https://openalex.org/W2028133023","https://openalex.org/W2029798646","https://openalex.org/W2031017369","https://openalex.org/W2034171869","https://openalex.org/W2038602542","https://openalex.org/W2038697935","https://openalex.org/W2046534438","https://openalex.org/W2049122170","https://openalex.org/W2050391960","https://openalex.org/W2053418558","https://openalex.org/W2055043387","https://openalex.org/W2057581357","https://openalex.org/W2067093784","https://openalex.org/W2070449043","https://openalex.org/W2081413793","https://openalex.org/W2088682178","https://openalex.org/W2089841008","https://openalex.org/W2094046904","https://openalex.org/W2094074715","https://openalex.org/W2094403030","https://openalex.org/W2099597342","https://openalex.org/W2100040827","https://openalex.org/W2108595383","https://openalex.org/W2129108032","https://openalex.org/W2130227962","https://openalex.org/W2143968827","https://openalex.org/W2144112248","https://openalex.org/W2152558091","https://openalex.org/W2156224334","https://openalex.org/W2269886648","https://openalex.org/W2409438444","https://openalex.org/W2461830693"],"related_works":["https://openalex.org/W2023661816","https://openalex.org/W1515431754","https://openalex.org/W2308472860","https://openalex.org/W1990787705","https://openalex.org/W2035189018","https://openalex.org/W2758330","https://openalex.org/W2053303814","https://openalex.org/W1599420609","https://openalex.org/W7098949956","https://openalex.org/W2104581765"],"abstract_inverted_index":{"Glutamate":[0],"receptors":[1,8,21],"are":[2,136],"the":[3,82,86,108,139,143,158,162,173,176,180,197,202,220,251],"most":[4],"abundant":[5],"excitatory":[6],"neurotransmitter":[7],"in":[9,64,68,101,138,157,237,257],"vertebrate":[10],"brain.":[11],"We":[12,78],"have":[13,59],"previously":[14,87,221],"cloned":[15],"cDNAs":[16],"encoding":[17],"two":[18],"homologous":[19],"kainate":[20],"(GFKAR":[22],"alpha,":[23],"45":[24],"kDa,":[25],"and":[26,34,45,73,75,90,96,120,123,145,201,210],"GFKAR":[27,215],"beta,":[28],"41":[29],"kDa)":[30],"from":[31,117],"goldfish":[32],"brain":[33],"proposed":[35,88,222],"a":[36,128,147,228,240],"topology":[37,241],"with":[38,70,150,242],"three":[39,243],"transmembrane":[40,92,194,224,230,244],"domains":[41,245],"(Wo,":[42],"Z.":[43],"G.,":[44],"Oswald,":[46],"R.":[47],"E.":[48],"(1994)":[49],"Proc.":[50],"Natl.":[51],"Acad.":[52],"Sci.":[53],"U.S.A.":[54],"91,":[55],"7154-7158).":[56],"These":[57,232],"studies":[58],"been":[60],"extended":[61],"using":[62],"an":[63,151,190],"vitro":[65],"translation/translocation":[66],"system":[67],"conjunction":[69],"site-specific":[71,129],"antibodies":[72],"point":[74],"deletion":[76],"mutations.":[77],"report":[79],"here":[80],"that":[81,170,175,219,246],"entire":[83,113],"region":[84],"between":[85,196],"third":[89],"fourth":[91],"segments":[93,195],"is":[94,182,226],"translocated":[95],"likely":[97,183],"to":[98,166,184,255],"be":[99,125,167,185],"extracellular":[100,198],"mature":[102],"receptors.":[103,260],"This":[104],"was":[105,115,164],"based":[106],"on":[107],"following":[109],"results.":[110],"1)":[111],"The":[112],"segment":[114,225],"protected":[116],"Proteinase":[118],"K":[119],"trypsin":[121],"digestion":[122],"could":[124],"immunoprecipitated":[126],"by":[127],"antibody.":[130],"2)":[131],"Functional":[132],"sites":[133],"for":[134,155,250],"N-glycosylation":[135,156,209],"present":[137],"C-terminal":[140],"half":[141,160],"of":[142,161,179,193,208,214,239,253],"segment,":[144,163],"3)":[146],"mutation,":[148],"constructed":[149],"additional":[152],"consensus":[153],"site":[154],"N-terminal":[159],"found":[165],"glycosylated":[168,203],"at":[169],"site.":[171],"Given":[172],"fact":[174],"N":[177,199],"terminus":[178,200],"protein":[181],"extracellular,":[186],"this":[187],"would":[188],"place":[189],"even":[191],"number":[192],"segment.":[204],"In":[205],"addition,":[206],"results":[207,233],"proteolysis":[211],"protection":[212],"assays":[213],"alpha":[216],"mutations":[217],"indicated":[218],"second":[223],"not":[227],"true":[229],"domain.":[231],"provide":[234],"further":[235],"evidence":[236],"support":[238],"has":[247],"important":[248],"implications":[249],"relationship":[252],"structure":[254],"function":[256],"ionotropic":[258],"glutamate":[259]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
