{"id":"https://openalex.org/W2052551677","doi":"https://doi.org/10.1371/journal.pone.0005716","title":"Novel Application of Fluorescence Lifetime and Fluorescence Microscopy Enables Quantitative Access to Subcellular Dynamics in Plant Cells","display_name":"Novel Application of Fluorescence Lifetime and Fluorescence Microscopy Enables Quantitative Access to Subcellular Dynamics in Plant Cells","publication_year":2009,"publication_date":"2009-05-26","ids":{"openalex":"https://openalex.org/W2052551677","doi":"https://doi.org/10.1371/journal.pone.0005716","mag":"2052551677","pmid":"https://pubmed.ncbi.nlm.nih.gov/19492078","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2683565"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0005716","doi":"https://doi.org/10.1371/journal.pone.0005716","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0005716","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0005716&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0005716&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088683742","display_name":"Kirstin Elgass","orcid":"https://orcid.org/0000-0001-7747-2637","observed_orcids":["https://orcid.org/0000-0001-7747-2637"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Kirstin Elgass","raw_affiliation_strings":["Institute for Physical and Theoretical Chemistry, University of T\u00fcbingen, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Physical and Theoretical Chemistry, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063752357","display_name":"Katharina Caesar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Katharina Caesar","raw_affiliation_strings":["Center for Plant Molecular Biology, Department of Plant Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Plant Molecular Biology, Department of Plant Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012598199","display_name":"Frank E. Schleifenbaum","orcid":"https://orcid.org/0000-0003-2138-3170","observed_orcids":["https://orcid.org/0000-0003-2138-3170"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Frank Schleifenbaum","raw_affiliation_strings":["Center for Plant Molecular Biology, Department of Plant Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany","Institute for Physical and Theoretical Chemistry, University of T\u00fcbingen, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Plant Molecular Biology, Department of Plant Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]},{"raw_affiliation_string":"Institute for Physical and Theoretical Chemistry, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067295491","display_name":"York\u2010Dieter Stierhof","orcid":"https://orcid.org/0000-0002-1784-1768","observed_orcids":["https://orcid.org/0000-0002-1784-1768"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"York-Dieter Stierhof","raw_affiliation_strings":["Center for Plant Molecular Biology, Microscopy, University of T\u00fcbingen, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Plant Molecular Biology, Microscopy, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082497361","display_name":"Alfred Johann Meixner","orcid":"https://orcid.org/0000-0002-0187-2906","observed_orcids":["https://orcid.org/0000-0002-0187-2906"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Alfred J. Meixner","raw_affiliation_strings":["Institute for Physical and Theoretical Chemistry, University of T\u00fcbingen, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Physical and Theoretical Chemistry, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065388692","display_name":"Klaus Harter","orcid":"https://orcid.org/0000-0002-2150-6970","observed_orcids":["https://orcid.org/0000-0002-2150-6970"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Klaus Harter","raw_affiliation_strings":["Center for Plant Molecular Biology, Department of Plant Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Plant Molecular Biology, Department of Plant Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5065388692","https://openalex.org/A5082497361"],"corresponding_institution_ids":["https://openalex.org/I8087733"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":3.05,"has_fulltext":true,"cited_by_count":37,"citation_normalized_percentile":{"value":0.91111601,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"4","issue":"5","first_page":"e5716","last_page":"e5716"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy","display_name":"fluorescence lifetime imaging","score":0.8379999995231628},{"id":"https://openalex.org/keywords/subcellular-dynamics","display_name":"subcellular dynamics","score":0.8119999766349792},{"id":"https://openalex.org/keywords/plant-cells","display_name":"plant cells","score":0.7860000133514404},{"id":"https://openalex.org/keywords/brassinosteroid-signaling","display_name":"brassinosteroid signaling","score":0.8610000014305115},{"id":"https://openalex.org/keywords/brassinolide","display_name":"brassinolide","score":0.8840000033378601},{"id":"https://openalex.org/keywords/cell-wall-expansion","display_name":"cell wall expansion","score":0.9919999837875366},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8640000224113464},{"id":"https://openalex.org/keywords/gfp-fusion-protein","display_name":"GFP fusion protein","score":0.8579999804496765},{"id":"https://openalex.org/keywords/in-vivo-monitoring","display_name":"in vivo monitoring","score":0.4350000023841858}],"concepts":[{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.7675982713699341},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.6720999479293823},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.6235268712043762},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.6226937174797058},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5717213153839111},{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.5204691886901855},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5200021862983704},{"id":"https://openalex.org/C192468462","wikidata":"https://www.wikidata.org/wiki/Q533543","display_name":"Chromophore","level":2,"score":0.4812983572483063},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.44371482729911804},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38219910860061646},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3284221887588501},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3030261993408203},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1733679175376892},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.08499601483345032},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002468","descriptor_name":"Cell Physiological Phenomena","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002473","descriptor_name":"Cell Wall","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002473","descriptor_name":"Cell Wall","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002473","descriptor_name":"Cell Wall","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D002777","descriptor_name":"Cholestanols","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013260","descriptor_name":"Steroids, Heterocyclic","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D018546","descriptor_name":"Hypocotyl","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D018546","descriptor_name":"Hypocotyl","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D018546","descriptor_name":"Hypocotyl","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D060406","descriptor_name":"Brassinosteroids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1371/journal.pone.0005716","doi":"https://doi.org/10.1371/journal.pone.0005716","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0005716","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0005716&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmid:19492078","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19492078","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2683565","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2683565/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.803.7560","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.803.7560","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/0f/c1/pone.0005716.PMC2683565.pdf","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:f0524730686a40a7b3eb6602f4ccb6a8","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/f0524730686a40a7b3eb6602f4ccb6a8","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 4, Iss 5, p e5716 (2009)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/147473","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/Novel_Application_of_Fluorescence_Lifetime_and_Fluorescence_Microscopy_Enables_Quantitative_Access_to_Subcellular_Dynamics_in_Plant_Cells/147473","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0005716","doi":"https://doi.org/10.1371/journal.pone.0005716","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0005716","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0005716&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2052551677.pdf","grobid_xml":"https://content.openalex.org/works/W2052551677.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W148837820","https://openalex.org/W1967100850","https://openalex.org/W1976987846","https://openalex.org/W1979218516","https://openalex.org/W1986901428","https://openalex.org/W1994207647","https://openalex.org/W1994442356","https://openalex.org/W2000350495","https://openalex.org/W2004108729","https://openalex.org/W2007378173","https://openalex.org/W2013669507","https://openalex.org/W2024387906","https://openalex.org/W2034507082","https://openalex.org/W2034541953","https://openalex.org/W2035530476","https://openalex.org/W2038294756","https://openalex.org/W2038792481","https://openalex.org/W2040144978","https://openalex.org/W2047205370","https://openalex.org/W2054455698","https://openalex.org/W2079968973","https://openalex.org/W2096946545","https://openalex.org/W2111430142","https://openalex.org/W2112454443","https://openalex.org/W2120014815","https://openalex.org/W2127068865","https://openalex.org/W2129995310","https://openalex.org/W2145757086","https://openalex.org/W2147655328","https://openalex.org/W2150264594","https://openalex.org/W2151001790","https://openalex.org/W2151477454","https://openalex.org/W2157207470","https://openalex.org/W2157294409","https://openalex.org/W2167267427","https://openalex.org/W2171224768","https://openalex.org/W2413103437"],"related_works":["https://openalex.org/W1498634963","https://openalex.org/W2055708944","https://openalex.org/W2039248768","https://openalex.org/W2086762161","https://openalex.org/W7164391961","https://openalex.org/W2117363434","https://openalex.org/W2023494692","https://openalex.org/W7171806681","https://openalex.org/W2467409397","https://openalex.org/W2092149757"],"abstract_inverted_index":{"BACKGROUND:":[0],"Optical":[1],"and":[2,21,38,73,95,114,133,141,166,194],"spectroscopic":[3],"technologies":[4,27,210],"working":[5],"at":[6,36,200],"subcellular":[7,39,168],"resolution":[8,97,202],"with":[9,92,182],"quantitative":[10,195],"output":[11],"are":[12,28,50,204],"required":[13],"for":[14,30,158,190],"a":[15,65,82,108,115],"deeper":[16],"understanding":[17],"of":[18,33,68,81,85,153,163,197],"molecular":[19],"processes":[20,199],"mechanisms":[22],"in":[23,44,56,98,102,119,124,127,135,160,191],"living":[24,99],"cells.":[25],"Such":[26],"prerequisite":[29],"the":[31,45,57,79,86,120,125,151,159,164],"realisation":[32],"predictive":[34],"biology":[35,55],"cellular":[37,198],"level.":[40],"However,":[41],"although":[42],"established":[43],"physical":[46],"sciences,":[47],"these":[48],"techniques":[49],"rarely":[51],"applied":[52],"to":[53,77],"cell":[54,111,130],"plant":[58],"sciences.":[59],"PRINCIPAL":[60],"FINDINGS:":[61],"Here,":[62],"we":[63],"present":[64],"combined":[66,181],"application":[67],"one-chromophore":[69,154],"fluorescence":[70,75,122,136,155,178,184],"lifetime":[71,123,137,156,179],"microscopy":[72,76,157],"wavelength-selective":[74],"analyse":[78],"function":[80],"GFP":[83,171],"fusion":[84,172],"Brassinosteroid":[87],"Insensitive":[88],"1":[89],"Receptor":[90],"(BRI1-GFP)":[91],"high":[93,201],"spatial":[94],"temporal":[96],"Arabidopsis":[100],"cells":[101],"their":[103],"tissue":[104],"environment.":[105],"We":[106],"show":[107,150],"rapid,":[109],"brassinolide-induced":[110],"wall":[112,131],"expansion":[113,132],"fast":[116],"BR-regulated":[117],"change":[118],"BRI1-GFP":[121],"plasmamembrane":[126],"vivo.":[128],"Both":[129],"changes":[134],"reflect":[138],"early":[139],"BR-induced":[140],"BRI1-dependent":[142],"physiological":[143],"or":[144,211],"signalling":[145],"processes.":[146],"Our":[147],"experiments":[148],"also":[149],"potential":[152],"vivo":[161,192],"monitoring":[162],"biochemical":[165],"biophysical":[167],"environment":[169],"using":[170],"proteins":[173],"as":[174],"probes.":[175],"SIGNIFICANCE:":[176],"One-chromophore":[177],"microscopy,":[180,185],"wavelength-specific":[183],"opens":[186],"up":[187],"new":[188],"frontiers":[189],"dynamic":[193],"analysis":[196],"which":[203],"not":[205],"addressable":[206],"by":[207],"pure":[208],"imaging":[209],"transmission":[212],"electron":[213],"microscopy.":[214]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}