{"id":"https://openalex.org/W2052023686","doi":"https://doi.org/10.1021/bi00067a026","title":"Mapping the S' subsites of serine proteases using acyl transfer to mixtures of peptide nucleophiles","display_name":"Mapping the S' subsites of serine proteases using acyl transfer to mixtures of peptide nucleophiles","publication_year":1993,"publication_date":"1993-04-27","ids":{"openalex":"https://openalex.org/W2052023686","doi":"https://doi.org/10.1021/bi00067a026","mag":"2052023686","pmid":"https://pubmed.ncbi.nlm.nih.gov/8476865"},"language":"en","primary_location":{"id":"doi:10.1021/bi00067a026","doi":"https://doi.org/10.1021/bi00067a026","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00067a026","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018783910","display_name":"Volker Schellenberger","orcid":"https://orcid.org/0000-0002-3491-8229","observed_orcids":["https://orcid.org/0000-0002-3491-8229"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Volker Schellenberger","raw_affiliation_strings":["Hormone Research Institute, University of California, San Francisco 94143"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hormone Research Institute, University of California, San Francisco 94143","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080063862","display_name":"Christoph W. Turck","orcid":"https://orcid.org/0000-0002-6585-8636","observed_orcids":["https://orcid.org/0000-0002-6585-8636"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Christoph W. Turck","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083404534","display_name":"Lizbeth Hedstrom","orcid":"https://orcid.org/0000-0001-8432-3322","observed_orcids":["https://orcid.org/0000-0001-8432-3322"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lizbeth Hedstrom","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5036382303","display_name":"William J. Rutter","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"William J. Rutter","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":4.8043,"has_fulltext":false,"cited_by_count":81,"citation_normalized_percentile":{"value":0.96060554,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"32","issue":"16","first_page":"4349","last_page":"4353"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9550999999046326,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9550999999046326,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.011300000362098217,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.006500000134110451,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.8730000257492065},{"id":"https://openalex.org/keywords/acyl-transfer","display_name":"acyl transfer","score":0.8519999980926514},{"id":"https://openalex.org/keywords/chymotrypsin","display_name":"chymotrypsin","score":0.9760000109672546},{"id":"https://openalex.org/keywords/trypsin","display_name":"trypsin","score":1.0}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8660781383514404},{"id":"https://openalex.org/C178907741","wikidata":"https://www.wikidata.org/wiki/Q745930","display_name":"Nucleophile","level":3,"score":0.8133267164230347},{"id":"https://openalex.org/C2778401398","wikidata":"https://www.wikidata.org/wiki/Q383836","display_name":"Chymotrypsin","level":4,"score":0.7125840783119202},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.6657794713973999},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6403018236160278},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5158366560935974},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.4891364872455597},{"id":"https://openalex.org/C193557335","wikidata":"https://www.wikidata.org/wiki/Q61424","display_name":"Acylation","level":3,"score":0.4851458966732025},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.44481346011161804},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.2825341820716858},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.28053802251815796},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17367395758628845},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.17208093404769897}],"mesh":[{"descriptor_ui":"D000217","descriptor_name":"Acyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002918","descriptor_name":"Chymotrypsin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002918","descriptor_name":"Chymotrypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00067a026","doi":"https://doi.org/10.1021/bi00067a026","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00067a026","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:8476865","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8476865","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1727563152","display_name":null,"funder_award_id":"NCRR RR01614","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G5334010142","display_name":"Bio-Organic Biomedical Mass Spectrometry Resource","funder_award_id":"P41RR001614","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G6016573372","display_name":null,"funder_award_id":"DK21344","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320332622","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W151902730","https://openalex.org/W1520137283","https://openalex.org/W1526936134","https://openalex.org/W1586570074","https://openalex.org/W1595939586","https://openalex.org/W1597564353","https://openalex.org/W1889077695","https://openalex.org/W1985469117","https://openalex.org/W1990566833","https://openalex.org/W1993973444","https://openalex.org/W1996427305","https://openalex.org/W1997509365","https://openalex.org/W2001262910","https://openalex.org/W2006446936","https://openalex.org/W2010649076","https://openalex.org/W2031222768","https://openalex.org/W2031817642","https://openalex.org/W2041719513","https://openalex.org/W2055151600","https://openalex.org/W2062819748","https://openalex.org/W2062874398","https://openalex.org/W2066837313","https://openalex.org/W2073723813","https://openalex.org/W2080262597","https://openalex.org/W2088855126","https://openalex.org/W2134567498","https://openalex.org/W2159511216"],"related_works":["https://openalex.org/W2060587633","https://openalex.org/W2001262910","https://openalex.org/W2056611341","https://openalex.org/W2032920834","https://openalex.org/W2007609350","https://openalex.org/W1971714630","https://openalex.org/W2089314802","https://openalex.org/W1993973444","https://openalex.org/W2035860907","https://openalex.org/W2418122357"],"abstract_inverted_index":{"We":[0],"have":[1,170,238],"developed":[2],"a":[3,35,69,80,93,163],"rapid":[4],"and":[5,92,141,168],"convenient":[6],"procedure":[7],"for":[8,59,152],"the":[9,12,27,30,53,63,85,126,153,220,230],"characterization":[10],"of":[11,16,21,32,34,52,77,89,125,165,177,219,232,242],"S'":[13],"subsite":[14,87],"specificity":[15,88,106,218,231],"serine":[17],"proteases.":[18],"A":[19],"mixture":[20,64],"peptide":[22,123],"nucleophiles":[23,124,159],"is":[24,45,184,227],"incubated":[25],"with":[26,103],"enzyme":[28],"in":[29,41,62,179,213],"presence":[31],"excess":[33],"specific":[36],"ester":[37],"substrate.":[38],"The":[39,175,216],"decrease":[40],"each":[42,60],"nucleophile":[43,61],"concentration":[44],"monitored":[46],"by":[47,132],"high-performance":[48],"liquid":[49],"chromatography":[50],"analysis":[51],"dansylated":[54],"mixture.":[55],"Relative":[56,148],"kinetic":[57],"parameters":[58],"are":[65,208],"then":[66],"calculated":[67],"using":[68],"new":[70],"statistical":[71],"algorithm":[72],"that":[73],"relates":[74],"all":[75,142],"pairs":[76],"nucleophiles.":[78],"As":[79],"first":[81],"application,":[82],"we":[83],"investigated":[84],"S'1":[86,173,217],"chymotrypsin,":[90],"trypsin,":[91],"recently":[94],"described":[95],"trypsin":[96,169],"mutant,":[97],"Tr-->Ch[S1":[98,222],"+":[99,101,223,225],"L1":[100,224],"L2]":[102,226],"chymotrypsin-like":[104],"primary":[105],"[Hedstrom,":[107],"L.,":[108,110],"Szilagyi,":[109],"&":[111],"Rutter,":[112],"W.":[113],"J.":[114],"(1992)":[115],"Science":[116],"255,":[117],"1249-1253].":[118],"For":[119],"this":[120],"purpose":[121],"21":[122],"general":[127],"structure":[128],"H-Xaa-Ala-Ala-Ala-Ala-NH2":[129],"were":[130,160],"prepared":[131],"multiple":[133],"solid-phase":[134],"synthesis,":[135],"where":[136],"Xaa":[137],"represents":[138],"D-alanine,":[139],"citrulline,":[140],"natural":[143],"amino":[144],"acids":[145],"except":[146],"cysteine.":[147],"second-order":[149],"rate":[150],"constants":[151],"enzyme-catalyzed":[154],"acyl":[155,181],"transfer":[156,182],"to":[157,229],"these":[158],"determined":[161],"over":[162],"range":[164],"10(2).":[166],"Chymotrypsin":[167],"markedly":[171],"different":[172],"specificities.":[174],"order":[176],"preference":[178],"chymotrypsin-catalyzed":[180],"reactions":[183],"positively":[185],"charged":[186],">":[187,189],"aliphatic":[188,206],"aromatic":[190,211,236],">>":[191],"negatively":[192],"charged,":[193],"D-Ala,":[194],"Pro":[195],"P'1":[196,214,235],"side":[197],"chain.":[198],"Trypsin":[199],"prefers":[200],"hydrophobic":[201],"residues,":[202],"but":[203],"like":[204],"chymotrypsin":[205],"residues":[207,212,237],"better":[209],"than":[210],"position.":[215],"mutant":[221],"similar":[228],"trypsin;":[233],"however,":[234],"low":[239],"reactivity":[240],"characteristic":[241],"chymotrypsin.":[243]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}