{"id":"https://openalex.org/W2052000490","doi":"https://doi.org/10.1073/pnas.0702172104","title":"Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved","display_name":"Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved","publication_year":2007,"publication_date":"2007-07-02","ids":{"openalex":"https://openalex.org/W2052000490","doi":"https://doi.org/10.1073/pnas.0702172104","mag":"2052000490","pmid":"https://pubmed.ncbi.nlm.nih.gov/17606918","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1924579"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.0702172104","doi":"https://doi.org/10.1073/pnas.0702172104","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.0702172104","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1924579","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101840591","display_name":"Mi\u2010Yeon Kim","orcid":"https://orcid.org/0000-0001-7440-5025","observed_orcids":["https://orcid.org/0000-0001-7440-5025"]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Miyeon Kim","raw_affiliation_strings":["Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319","Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319","institution_ids":["https://openalex.org/I51556381"]},{"raw_affiliation_string":"Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319, USA","institution_ids":["https://openalex.org/I51556381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086992798","display_name":"Gail E. Fanucci","orcid":"https://orcid.org/0000-0003-1960-160X","observed_orcids":["https://orcid.org/0000-0003-1960-160X"]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gail E. Fanucci","raw_affiliation_strings":["Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319","institution_ids":["https://openalex.org/I51556381"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028973291","display_name":"David S. Cafiso","orcid":"https://orcid.org/0000-0002-3813-8721","observed_orcids":["https://orcid.org/0000-0002-3813-8721"]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David S. Cafiso","raw_affiliation_strings":["Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, VA 22904-4319","institution_ids":["https://openalex.org/I51556381"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I51556381"],"apc_list":null,"apc_paid":null,"fwci":2.1249,"has_fulltext":false,"cited_by_count":38,"citation_normalized_percentile":{"value":0.87991774,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"104","issue":"29","first_page":"11975","last_page":"11980"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9018999934196472,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9018999934196472,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0348999984562397,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12381","display_name":"Electron Spin Resonance Studies","score":0.026799999177455902,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tonb-dependent-transporters","display_name":"TonB-dependent transporters","score":0.968999981880188},{"id":"https://openalex.org/keywords/tonb","display_name":"TonB","score":0.8019999861717224},{"id":"https://openalex.org/keywords/btub","display_name":"BtuB","score":0.8240000009536743},{"id":"https://openalex.org/keywords/feca","display_name":"FecA","score":0.9940000176429749},{"id":"https://openalex.org/keywords/fhua","display_name":"FhuA","score":0.996999979019165},{"id":"https://openalex.org/keywords/protein-conformational-dynamics","display_name":"protein conformational dynamics","score":0.6899999976158142},{"id":"https://openalex.org/keywords/bacterial-outer-membrane","display_name":"bacterial outer membrane","score":0.4950000047683716},{"id":"https://openalex.org/keywords/site-directed-spin-labeling","display_name":"site-directed spin labeling","score":0.7059999704360962}],"concepts":[{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.6956335306167603},{"id":"https://openalex.org/C146587185","wikidata":"https://www.wikidata.org/wiki/Q258248","display_name":"Bacterial outer membrane","level":4,"score":0.6136888265609741},{"id":"https://openalex.org/C118892022","wikidata":"https://www.wikidata.org/wiki/Q7834587","display_name":"Transmembrane domain","level":3,"score":0.6003872156143188},{"id":"https://openalex.org/C115335371","wikidata":"https://www.wikidata.org/wiki/Q142943","display_name":"Membrane transport protein","level":4,"score":0.5551175475120544},{"id":"https://openalex.org/C57581600","wikidata":"https://www.wikidata.org/wiki/Q2449730","display_name":"Transport protein","level":2,"score":0.5375608205795288},{"id":"https://openalex.org/C177462420","wikidata":"https://www.wikidata.org/wiki/Q7531731","display_name":"Site-directed spin labeling","level":3,"score":0.526929497718811},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.5179364085197449},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.5144315361976624},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5121955275535583},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.49101686477661133},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.47644880414009094},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4386154115200043},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39147207140922546},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.19519540667533875},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.09613871574401855},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004578","descriptor_name":"Electron Spin Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.0702172104","doi":"https://doi.org/10.1073/pnas.0702172104","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.0702172104","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1924579","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1924579","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:17606918","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17606918","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1924579","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1924579","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000892662","display_name":"Molecular Mechanisms of Membrane Transport","funder_award_id":"R01GM035215","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W68474599","https://openalex.org/W163411833","https://openalex.org/W1558837374","https://openalex.org/W1601331206","https://openalex.org/W1858237937","https://openalex.org/W1929389910","https://openalex.org/W1963676019","https://openalex.org/W1966106135","https://openalex.org/W1966854979","https://openalex.org/W1973583974","https://openalex.org/W1974635026","https://openalex.org/W1974700625","https://openalex.org/W1975121208","https://openalex.org/W1976022528","https://openalex.org/W1986651499","https://openalex.org/W1989128783","https://openalex.org/W1990111634","https://openalex.org/W1991385639","https://openalex.org/W2000984377","https://openalex.org/W2006192061","https://openalex.org/W2006580038","https://openalex.org/W2007760903","https://openalex.org/W2007921389","https://openalex.org/W2008812223","https://openalex.org/W2012925002","https://openalex.org/W2015577465","https://openalex.org/W2025346903","https://openalex.org/W2031534307","https://openalex.org/W2039369649","https://openalex.org/W2039722695","https://openalex.org/W2044186322","https://openalex.org/W2053108297","https://openalex.org/W2069610748","https://openalex.org/W2072871401","https://openalex.org/W2076272075","https://openalex.org/W2080738203","https://openalex.org/W2084608838","https://openalex.org/W2090678119","https://openalex.org/W2100118286","https://openalex.org/W2108623931","https://openalex.org/W2151319524","https://openalex.org/W2152218729","https://openalex.org/W2163483796","https://openalex.org/W3144201415"],"related_works":["https://openalex.org/W2585187796","https://openalex.org/W2785168947","https://openalex.org/W1982699178","https://openalex.org/W4243422931","https://openalex.org/W1980562288","https://openalex.org/W1558837374","https://openalex.org/W2049076695","https://openalex.org/W2593626773","https://openalex.org/W2032764052","https://openalex.org/W2094990028"],"abstract_inverted_index":{"Site-directed":[0],"spin":[1],"labeling":[2],"(SDSL)":[3],"was":[4],"used":[5],"to":[6,31,41,74,126],"examine":[7],"and":[8,21,130],"compare":[9],"transmembrane":[10],"signaling":[11],"events":[12],"in":[13,53,100,141,147,173],"the":[14,32,45,57,65,79,101,120,156,182],"bacterial":[15],"outer-membrane":[16],"transport":[17,28],"proteins":[18,24],"BtuB,":[19,78],"FecA,":[20,61],"FhuA.":[22,149],"These":[23],"extract":[25],"energy":[26],"for":[27,77,165],"by":[29,44,181],"coupling":[30],"transperiplasmic":[33],"protein":[34,92],"TonB,":[35,168],"an":[36,87],"interaction":[37],"that":[38,64,75,137,155,170],"is":[39,124],"thought":[40],"be":[42,127,163],"mediated":[43],"Ton":[46,66,121,157,183],"box,":[47],"a":[48,69,109],"highly":[49,90,128],"conserved":[50],"energy-coupling":[51],"motif":[52],"these":[54,152],"transporters.":[55],"In":[56,113,133],"ferric":[58],"citrate":[59],"transporter,":[60,103],"SDSL":[62,105,135],"indicates":[63,136],"box":[67,122,158,184],"undergoes":[68],"substrate-induced":[70,110],"disorder":[71],"transition":[72],"similar":[73],"seen":[76,140],"vitamin":[80],"B(12)":[81],"transporter.":[82],"This":[83],"conformational":[84],"change":[85],"produces":[86],"aqueous":[88],"exposed,":[89],"disordered":[91],"fragment,":[93],"which":[94],"likely":[95],"regulates":[96],"transporter-TonB":[97,171],"interactions.":[98],"However,":[99],"ferrichrome":[102],"FhuA,":[104],"does":[106],"not":[107,145,179],"reveal":[108],"unfolding":[111],"transition.":[112],"this":[114],"protein,":[115],"with":[116,167],"or":[117,178],"without":[118],"substrate,":[119],"conformation":[123],"found":[125,146],"dynamic":[129],"constitutively":[131],"unfolded.":[132],"addition,":[134],"structural":[138],"features":[139],"high-resolution":[142],"models":[143],"are":[144,175],"membrane-associated":[148],"Taken":[150],"together,":[151],"data":[153],"indicate":[154],"of":[159],"FhuA":[160,174],"may":[161],"always":[162],"available":[164],"interactions":[166,172],"implying":[169],"either":[176],"constitutive":[177],"regulated":[180],"configuration.":[185]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}