{"id":"https://openalex.org/W2051663745","doi":"https://doi.org/10.1074/jbc.m512787200","title":"Kinetic Analysis of the Ku-DNA Binding Activity Reveals a Redox-dependent Alteration in Protein Structure That Stimulates Dissociation of the Ku-DNA Complex","display_name":"Kinetic Analysis of the Ku-DNA Binding Activity Reveals a Redox-dependent Alteration in Protein Structure That Stimulates Dissociation of the Ku-DNA Complex","publication_year":2006,"publication_date":"2006-03-15","ids":{"openalex":"https://openalex.org/W2051663745","doi":"https://doi.org/10.1074/jbc.m512787200","mag":"2051663745","pmid":"https://pubmed.ncbi.nlm.nih.gov/16537541","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2432111"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m512787200","doi":"https://doi.org/10.1074/jbc.m512787200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m512787200","pdf_url":"http://www.jbc.org/article/S0021925819748996/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925819748996/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065119590","display_name":"Brooke J. Andrews","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brooke J. Andrews","raw_affiliation_strings":["Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202","institution_ids":["https://openalex.org/I4394709130"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013404192","display_name":"Jason A. Lehman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jason A. Lehman","raw_affiliation_strings":["Department of Biomedical Sciences, Wright State University, Dayton, Ohio 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, Wright State University, Dayton, Ohio 45435","institution_ids":["https://openalex.org/I19648265"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005809670","display_name":"John J. Turchi","orcid":"https://orcid.org/0000-0001-5375-2992","observed_orcids":["https://orcid.org/0000-0001-5375-2992"]},"institutions":[{"id":"https://openalex.org/I4388446366","display_name":"Indiana University Indianapolis","ror":"https://ror.org/03eftgw80","country_code":"US","type":"education","lineage":["https://openalex.org/I4388446366","https://openalex.org/I592451"]},{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]},{"id":"https://openalex.org/I55769427","display_name":"Indiana University \u2013 Purdue University Indianapolis","ror":"https://ror.org/05gxnyn08","country_code":"US","type":"education","lineage":["https://openalex.org/I55769427","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John J. Turchi","raw_affiliation_strings":["Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202. Electronic address: jturchi@iupui.edu","Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202","Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202. Electronic address: jturchi@iupui.edu","institution_ids":["https://openalex.org/I4388446366","https://openalex.org/I4394709130","https://openalex.org/I55769427"]},{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202","institution_ids":["https://openalex.org/I4394709130"]},{"raw_affiliation_string":"Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202","institution_ids":["https://openalex.org/I4394709130"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5005809670"],"corresponding_institution_ids":["https://openalex.org/I4388446366","https://openalex.org/I4394709130","https://openalex.org/I55769427"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.5614,"has_fulltext":true,"cited_by_count":49,"citation_normalized_percentile":{"value":0.58491411,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"281","issue":"19","first_page":"13596","last_page":"13603"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ku-protein","display_name":"Ku protein","score":0.9309999942779541},{"id":"https://openalex.org/keywords/redox-regulation","display_name":"redox regulation","score":0.8669999837875366},{"id":"https://openalex.org/keywords/non-homologous-end-joining","display_name":"non-homologous end joining","score":0.9409999847412109},{"id":"https://openalex.org/keywords/double-strand-break-repair","display_name":"DNA double-strand break repair","score":0.949999988079071},{"id":"https://openalex.org/keywords/pre-steady-state-kinetics","display_name":"pre-steady-state kinetics","score":0.7979999780654907},{"id":"https://openalex.org/keywords/fluorescence-quenching","display_name":"fluorescence quenching","score":0.8569999933242798},{"id":"https://openalex.org/keywords/protein-structure","display_name":"protein structure","score":0.3659999966621399},{"id":"https://openalex.org/keywords/diamide","display_name":"diamide","score":0.6029999852180481},{"id":"https://openalex.org/keywords/dithiothreitol","display_name":"dithiothreitol","score":0.9359999895095825},{"id":"https://openalex.org/keywords/limited-proteolysis","display_name":"limited proteolysis","score":0.7419999837875366},{"id":"https://openalex.org/keywords/ku80","display_name":"Ku80","score":0.5320000052452087},{"id":"https://openalex.org/keywords/ku70","display_name":"Ku70","score":0.9900000095367432}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7267284989356995},{"id":"https://openalex.org/C55904794","wikidata":"https://www.wikidata.org/wiki/Q82682","display_name":"Redox","level":2,"score":0.6801462769508362},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5445206165313721},{"id":"https://openalex.org/C2780919815","wikidata":"https://www.wikidata.org/wiki/Q6440930","display_name":"Ku70","level":4,"score":0.48568519949913025},{"id":"https://openalex.org/C2779270785","wikidata":"https://www.wikidata.org/wiki/Q300902","display_name":"TCEP","level":4,"score":0.44077789783477783},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.39632803201675415},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3872942328453064},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.35646331310272217},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2568771541118622},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C2777716191","wikidata":"https://www.wikidata.org/wiki/Q207877","display_name":"Phosphine","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000072200","descriptor_name":"Ku Autoantigen","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004265","descriptor_name":"DNA Helicases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1074/jbc.m512787200","doi":"https://doi.org/10.1074/jbc.m512787200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m512787200","pdf_url":"http://www.jbc.org/article/S0021925819748996/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:16537541","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16537541","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2432111","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2432111","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Biol Chem","raw_type":"Text"},{"id":"pmh:oai:scholarworks.indianapolis.iu.edu:1805/55503","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/1805/55503","pdf_url":null,"source":{"id":"https://openalex.org/S4306400987","display_name":"IUScholarWorks (Indiana University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I592451","host_organization_name":"Indiana University","host_organization_lineage":["https://openalex.org/I592451"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PMC","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m512787200","doi":"https://doi.org/10.1074/jbc.m512787200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m512787200","pdf_url":"http://www.jbc.org/article/S0021925819748996/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3707751629","display_name":"Recognition and Repair of Cisplatin-DNA Damage","funder_award_id":"R01CA082741","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306078","display_name":"U.S. Department of Defense","ror":"https://ror.org/0447fe631"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2051663745.pdf","grobid_xml":"https://content.openalex.org/works/W2051663745.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W16758304","https://openalex.org/W92983960","https://openalex.org/W1516845708","https://openalex.org/W1557970645","https://openalex.org/W1964867778","https://openalex.org/W1974224154","https://openalex.org/W1977273560","https://openalex.org/W1979726641","https://openalex.org/W1985679430","https://openalex.org/W1999037466","https://openalex.org/W2008864694","https://openalex.org/W2011320725","https://openalex.org/W2015006197","https://openalex.org/W2015339656","https://openalex.org/W2033270381","https://openalex.org/W2045526190","https://openalex.org/W2049878642","https://openalex.org/W2052349609","https://openalex.org/W2053311488","https://openalex.org/W2061015483","https://openalex.org/W2062513246","https://openalex.org/W2066180009","https://openalex.org/W2069651213","https://openalex.org/W2071695004","https://openalex.org/W2091097441","https://openalex.org/W2096628134","https://openalex.org/W2097517187","https://openalex.org/W2111802021","https://openalex.org/W2114745290","https://openalex.org/W2127173305","https://openalex.org/W2142935483","https://openalex.org/W2148330718","https://openalex.org/W2149912948","https://openalex.org/W2162412306","https://openalex.org/W2177380697","https://openalex.org/W2188227426","https://openalex.org/W2417226089","https://openalex.org/W4254273459"],"related_works":["https://openalex.org/W2090103244","https://openalex.org/W2273724537","https://openalex.org/W2325442151","https://openalex.org/W2417226089","https://openalex.org/W1999271329","https://openalex.org/W2060734146","https://openalex.org/W1971939906","https://openalex.org/W3030061889","https://openalex.org/W2040847982","https://openalex.org/W1516845708"],"abstract_inverted_index":{"Ku":[0,33,49,56,96,116,124,135,164,179,212,233],"is":[1,205,237,243],"a":[2,41,71,80,120,152,157,188,208,227],"heterodimeric":[3],"protein":[4],"comprising":[5],"70-":[6],"and":[7,39,61,145,180,220,242],"80-kDa":[8],"subunits":[9],"that":[10,236],"participate":[11],"in":[12,70,134,141,160,202,211],"the":[13,28,106,128,142,161,193,196,230,247,250],"non-homologous":[14],"end-joining":[15],"(NHEJ)":[16],"repair":[17],"pathway":[18],"for":[19,229],"rejoining":[20],"DNA":[21,36,50,60,136,167,235],"double":[22],"strand":[23],"breaks.":[24],"We":[25],"have":[26],"analyzed":[27],"pre-steady":[29],"state":[30,46],"binding":[31,51,59,68,82,99,118,137],"of":[32,48,95,154,163,178,195,232,249],"with":[34,234],"various":[35],"duplex":[37],"substrates":[38],"identified":[40],"redox-sensitive":[42],"Ku-DNA":[43,197,203,251],"interaction.":[44],"Pre-steady":[45],"analysis":[47],"was":[52,168,172,215],"monitored":[53],"via":[54],"intrinsic":[55],"quenching":[57,94,103],"upon":[58,98],"revealed":[62,79],"that,":[63],"under":[64,76,85,105],"fully":[65,86],"reduced":[66],"conditions,":[67,88,156],"occurred":[69],"single-step":[72],"process.":[73],"Reactions":[74],"performed":[75],"limited":[77,218],"reduction":[78],"two-step":[81],"process,":[83],"whereas":[84],"oxidized":[87],"we":[89],"were":[90,139],"unable":[91],"to":[92,114,119],"detect":[93],"fluorescence":[97],"DNA.":[100],"The":[101,199,224],"differential":[102],"observed":[104,140],"different":[107],"redox":[108,155,190,240],"conditions":[109],"could":[110,181],"not":[111],"be":[112,182],"attributed":[113],"two":[115],"molecules":[117],"single":[121],"substrate":[122],"or":[123],"sliding":[125],"inward":[126],"on":[127,192],"substrate.":[129],"Although":[130],"only":[131],"modest":[132],"differences":[133],"activity":[138],"stoichiometric":[143],"anisotropy":[144],"electrophoretic":[146],"mobility":[147],"shift":[148],"assay":[149],"studies,":[150],"as":[151],"function":[153],"dramatic":[158],"difference":[159],"rate":[162],"dissociation":[165,248],"from":[166],"observed.":[169],"This":[170],"effect":[171,191],"also":[173],"induced":[174],"by":[175,184,207,217,239,245],"diamide":[176],"treatment":[177],"abrogated":[183],"dithiothreitol":[185],"treatment,":[186],"demonstrating":[187],"reversible":[189],"stability":[194],"complex.":[198,252],"redox-dependent":[200,209],"alteration":[201,210],"interactions":[204],"manifested":[206],"structure,":[213],"which":[214],"confirmed":[216],"proteolysis":[219],"mass":[221],"spectrometry":[222],"analyses.":[223],"results":[225],"support":[226],"model":[228],"interaction":[231],"regulated":[238],"status":[241],"achieved":[244],"altering":[246]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":8}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}