{"id":"https://openalex.org/W2051643501","doi":"https://doi.org/10.1002/bms.1200221102","title":"On-line post-column Diels-Alder derivatization for the determination of vitamin D3 and its metabolites by liquid chromatography/thermospray mass spectrometry","display_name":"On-line post-column Diels-Alder derivatization for the determination of vitamin D3 and its metabolites by liquid chromatography/thermospray mass spectrometry","publication_year":1993,"publication_date":"1993-11-01","ids":{"openalex":"https://openalex.org/W2051643501","doi":"https://doi.org/10.1002/bms.1200221102","mag":"2051643501","pmid":"https://pubmed.ncbi.nlm.nih.gov/8251549"},"language":"en","primary_location":{"id":"doi:10.1002/bms.1200221102","doi":"https://doi.org/10.1002/bms.1200221102","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bms.1200221102","pdf_url":null,"source":{"id":"https://openalex.org/S37349105","display_name":"Journal of Mass Spectrometry","issn_l":"1076-5174","issn":["1076-5174","1096-9888"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079069511","display_name":"Rob J. Vreeken","orcid":"https://orcid.org/0000-0003-3568-1371","observed_orcids":["https://orcid.org/0000-0003-3568-1371"]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"R. J. Vreeken","raw_affiliation_strings":["Department of Analytical Chemistry, Free University, Amsterdam, The Netherlands","Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102978022","display_name":"Maarten Honing","orcid":"https://orcid.org/0000-0001-7180-6245","observed_orcids":["https://orcid.org/0000-0001-7180-6245"]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"M. Honing","raw_affiliation_strings":["Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110157497","display_name":"Ben L. M. van Baar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"B. L. M. van Baar","raw_affiliation_strings":["Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015009595","display_name":"Rudy T. Ghijsen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"R. T. Ghijsen","raw_affiliation_strings":["Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111872639","display_name":"Gerhardus J. de Jong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"G. J. de Jong","raw_affiliation_strings":["Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108419076","display_name":"Udo A. Th. Brinkman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"U. A. Th. Brinkman","raw_affiliation_strings":["Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]},{"raw_affiliation_string":"Department of Organic Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5079069511"],"corresponding_institution_ids":["https://openalex.org/I865915315"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":1.3208,"has_fulltext":false,"cited_by_count":37,"citation_normalized_percentile":{"value":0.77268655,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"22","issue":"11","first_page":"621","last_page":"632"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.8695999979972839,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.8695999979972839,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.1005999967455864,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.010499999858438969,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vitamin-d3","display_name":"vitamin D3","score":0.9940000176429749},{"id":"https://openalex.org/keywords/vitamin-d-metabolites","display_name":"vitamin D metabolites","score":0.9789999723434448},{"id":"https://openalex.org/keywords/diels-alder-reaction","display_name":"Diels-Alder reaction","score":0.9819999933242798},{"id":"https://openalex.org/keywords/thermospray-mass-spectrometry","display_name":"thermospray mass spectrometry","score":0.9319999814033508},{"id":"https://openalex.org/keywords/liquid-chromatography","display_name":"liquid chromatography","score":0.5669999718666077},{"id":"https://openalex.org/keywords/1-25-dihydroxyvitamin-d3","display_name":"1,25-dihydroxyvitamin D3","score":0.8220000267028809},{"id":"https://openalex.org/keywords/25-hydroxyvitamin-d3","display_name":"25-hydroxyvitamin D3","score":0.9729999899864197},{"id":"https://openalex.org/keywords/1alpha-hydroxyvitamin-d3","display_name":"1\u03b1-hydroxyvitamin D3","score":0.8870000243186951},{"id":"https://openalex.org/keywords/24-25-dihydroxyvitamin-d3","display_name":"24,25-dihydroxyvitamin D3","score":0.9860000014305115},{"id":"https://openalex.org/keywords/negative-ion-detection","display_name":"negative ion detection","score":0.75},{"id":"https://openalex.org/keywords/regioisomers","display_name":"regioisomers","score":0.6570000052452087}],"concepts":[{"id":"https://openalex.org/C186716092","wikidata":"https://www.wikidata.org/wiki/Q3990339","display_name":"Thermospray","level":5,"score":0.9447802305221558},{"id":"https://openalex.org/C2776923573","wikidata":"https://www.wikidata.org/wiki/Q2294884","display_name":"Derivatization","level":3,"score":0.9268842935562134},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8426709175109863},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.8248312473297119},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.6992875337600708},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.6884336471557617},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6654201149940491},{"id":"https://openalex.org/C51884965","wikidata":"https://www.wikidata.org/wiki/Q471254","display_name":"Chemical ionization","level":4,"score":0.4735488295555115},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.4618493616580963},{"id":"https://openalex.org/C64489805","wikidata":"https://www.wikidata.org/wiki/Q873864","display_name":"Liquid chromatography\u2013mass spectrometry","level":3,"score":0.4536714553833008},{"id":"https://openalex.org/C2776940978","wikidata":"https://www.wikidata.org/wiki/Q34956","display_name":"Vitamin","level":2,"score":0.4444595277309418},{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.3127968907356262},{"id":"https://openalex.org/C198291218","wikidata":"https://www.wikidata.org/wiki/Q190382","display_name":"Ionization","level":3,"score":0.22695893049240112},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.20493796467781067},{"id":"https://openalex.org/C71043704","wikidata":"https://www.wikidata.org/wiki/Q17144692","display_name":"Selected reaction monitoring","level":4,"score":0.17420917749404907},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0957963764667511},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002762","descriptor_name":"Cholecalciferol","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bms.1200221102","doi":"https://doi.org/10.1002/bms.1200221102","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bms.1200221102","pdf_url":null,"source":{"id":"https://openalex.org/S37349105","display_name":"Journal of Mass Spectrometry","issn_l":"1076-5174","issn":["1076-5174","1096-9888"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:8251549","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8251549","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological mass spectrometry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W590764796","https://openalex.org/W629107560","https://openalex.org/W1483580501","https://openalex.org/W1504544933","https://openalex.org/W1511537762","https://openalex.org/W1528546590","https://openalex.org/W1599024555","https://openalex.org/W1964020568","https://openalex.org/W1966920819","https://openalex.org/W1977872425","https://openalex.org/W1983039354","https://openalex.org/W1990527227","https://openalex.org/W1997239483","https://openalex.org/W1997776389","https://openalex.org/W1999069832","https://openalex.org/W1999795927","https://openalex.org/W2003221727","https://openalex.org/W2006289398","https://openalex.org/W2011244100","https://openalex.org/W2011452755","https://openalex.org/W2015291122","https://openalex.org/W2020706257","https://openalex.org/W2029793661","https://openalex.org/W2030150020","https://openalex.org/W2032721551","https://openalex.org/W2035696576","https://openalex.org/W2044002585","https://openalex.org/W2056919047","https://openalex.org/W2062997049","https://openalex.org/W2065881195","https://openalex.org/W2067232524","https://openalex.org/W2067872720","https://openalex.org/W2069561619","https://openalex.org/W2073614670","https://openalex.org/W2078737842","https://openalex.org/W2079987591","https://openalex.org/W2081876226","https://openalex.org/W2083037890","https://openalex.org/W2088142244","https://openalex.org/W2105686632","https://openalex.org/W2111836913","https://openalex.org/W2142335026","https://openalex.org/W2161915094","https://openalex.org/W2316202874","https://openalex.org/W2463984628","https://openalex.org/W2887409801"],"related_works":["https://openalex.org/W2062997049","https://openalex.org/W4381050043","https://openalex.org/W2057725809","https://openalex.org/W4382933011","https://openalex.org/W4377564574","https://openalex.org/W2583673808","https://openalex.org/W2767521567","https://openalex.org/W2115218143","https://openalex.org/W1988319754","https://openalex.org/W2079777604"],"abstract_inverted_index":{"Liquid":[0],"chromatography/thermospray":[1],"mass":[2],"spectrometry":[3],"(LC/TSP":[4],"MS)":[5],"has":[6],"been":[7],"used":[8],"for":[9],"the":[10,53,61,105,109,117,139],"determination":[11],"of":[12,17,60,67,108],"vitamin":[13,23,26,30,34],"D3":[14,31],"and":[15,32,38],"some":[16],"its":[18],"metabolites,":[19],"i.e.":[20,129],"1":[21,28,92,127],"alpha(OH)":[22],"D3,":[24,27,35],"25(OH)":[25],"alpha,25(OH)2":[29],"24,25(OH)2":[33],"using":[36,69],"positive":[37],"negative":[39,54,140],"ion":[40,55,71,141],"detection.":[41],"Using":[42],"these":[43],"two":[44],"modes":[45],"positional":[46],"isomers":[47],"can":[48,111],"be":[49,112],"identified.":[50],"Detection":[51],"in":[52,102,138],"mode":[56],"was":[57],"preferred":[58],"because":[59],"slightly":[62],"higher":[63],"sensitivity.":[64],"The":[65,120],"limits":[66,107,124],"detection,":[68,72],"multiple":[70],"are":[73,133],"50-100":[74],"nM":[75],"(6-12":[76],"pmol":[77,131],"injected).":[78],"On-line":[79],"post-column":[80],"derivatization":[81],"based":[82],"on":[83,116],"[4":[84],"+":[85],"2]":[86],"cyclo-addition":[87],"(Diels-Alder":[88],"reaction)":[89],"proceeds":[90],"within":[91],"min":[93],"at":[94],"room":[95],"temperature.":[96],"If":[97],"this":[98],"step":[99],"is":[100],"included":[101],"LC/TSP":[103],"MS,":[104],"detection":[106],"analytes":[110],"improved":[113],"7-70-fold":[114],"depending":[115],"analyte":[118],"tested.":[119],"best":[121],"results":[122],"(detection":[123],"down":[125],"to":[126],"nM,":[128],"0.12":[130],"injected)":[132],"obtained":[134],"with":[135],"discharge":[136],"ionization":[137],"mode.":[142]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}