{"id":"https://openalex.org/W2050201307","doi":"https://doi.org/10.1139/y84-218","title":"The 3\u03b2-hydroxysteroid dehydrogenase activity of cultured porcine Leydig cells in primary culture","display_name":"The 3\u03b2-hydroxysteroid dehydrogenase activity of cultured porcine Leydig cells in primary culture","publication_year":1984,"publication_date":"1984-10-01","ids":{"openalex":"https://openalex.org/W2050201307","doi":"https://doi.org/10.1139/y84-218","mag":"2050201307","pmid":"https://pubmed.ncbi.nlm.nih.gov/6595051"},"language":"en","primary_location":{"id":"doi:10.1139/y84-218","doi":"https://doi.org/10.1139/y84-218","is_oa":false,"landing_page_url":"https://doi.org/10.1139/y84-218","pdf_url":null,"source":{"id":"https://openalex.org/S82722058","display_name":"Canadian Journal of Physiology and Pharmacology","issn_l":"0008-4212","issn":["0008-4212","1205-7541"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Physiology and Pharmacology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"M. Bernier","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M. Bernier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008972130","display_name":"William Gibb","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W. Gibb","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109965510","display_name":"J.M. Saez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J. M. Saez","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050023199","display_name":"ROBERT COLLU","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R. Collu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110888148","display_name":"JACQUES R. DUCHARME","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J. -R. Ducharme","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.1546614,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"62","issue":"10","first_page":"1300","last_page":"1303"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9609000086784363,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9609000086784363,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11146","display_name":"Hormonal and reproductive studies","score":0.014499999582767487,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10960","display_name":"Hypothalamic control of reproductive hormones","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/3beta-hydroxysteroid-dehydrogenase","display_name":"3\u03b2-hydroxysteroid dehydrogenase","score":0.9419999718666077},{"id":"https://openalex.org/keywords/primary-culture","display_name":"primary cell culture","score":0.8059999942779541},{"id":"https://openalex.org/keywords/testosterone-production","display_name":"testosterone production","score":0.6740000247955322},{"id":"https://openalex.org/keywords/human-chorionic-gonadotropin","display_name":"human chorionic gonadotropin","score":0.9229999780654907},{"id":"https://openalex.org/keywords/dehydroepiandrosterone","display_name":"dehydroepiandrosterone","score":0.8090000152587891},{"id":"https://openalex.org/keywords/androstenedione","display_name":"androstenedione","score":0.972000002861023},{"id":"https://openalex.org/keywords/enzyme-induction","display_name":"enzyme induction","score":0.609000027179718},{"id":"https://openalex.org/keywords/de-novo-protein-synthesis","display_name":"de novo protein synthesis","score":0.8360000252723694},{"id":"https://openalex.org/keywords/cycloheximide","display_name":"cycloheximide","score":0.9470000267028809},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5450000166893005},{"id":"https://openalex.org/keywords/steroidogenesis","display_name":"steroidogenesis","score":0.4650000035762787}],"concepts":[{"id":"https://openalex.org/C2778128677","wikidata":"https://www.wikidata.org/wiki/Q412895","display_name":"Cycloheximide","level":3,"score":0.9364663362503052},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.8058766722679138},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.805627703666687},{"id":"https://openalex.org/C2776992908","wikidata":"https://www.wikidata.org/wiki/Q408376","display_name":"Dehydroepiandrosterone","level":4,"score":0.7282718420028687},{"id":"https://openalex.org/C2775948956","wikidata":"https://www.wikidata.org/wiki/Q407172","display_name":"Human chorionic gonadotropin","level":3,"score":0.6193471550941467},{"id":"https://openalex.org/C2776683971","wikidata":"https://www.wikidata.org/wiki/Q411064","display_name":"Androstenedione","level":4,"score":0.5910069942474365},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5905954241752625},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.5513444542884827},{"id":"https://openalex.org/C2779279991","wikidata":"https://www.wikidata.org/wiki/Q6058681","display_name":"Testosterone (patch)","level":2,"score":0.48977112770080566},{"id":"https://openalex.org/C2777318727","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"Lactate dehydrogenase","level":3,"score":0.4779201149940491},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4473423957824707},{"id":"https://openalex.org/C2778782382","wikidata":"https://www.wikidata.org/wiki/Q409770","display_name":"Gonadotropin","level":3,"score":0.44391703605651855},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4434613287448883},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.39737236499786377},{"id":"https://openalex.org/C2777911890","wikidata":"https://www.wikidata.org/wiki/Q62614","display_name":"Androgen","level":3,"score":0.3282078504562378},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.24731257557868958},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21312183141708374},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.15617814660072327},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10855254530906677}],"mesh":[{"descriptor_ui":"D000735","descriptor_name":"Androstenedione","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003513","descriptor_name":"Cycloheximide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007985","descriptor_name":"Leydig Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011375","descriptor_name":"Progesterone Reductase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013739","descriptor_name":"Testosterone","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015096","descriptor_name":"3-Hydroxysteroid Dehydrogenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1139/y84-218","doi":"https://doi.org/10.1139/y84-218","is_oa":false,"landing_page_url":"https://doi.org/10.1139/y84-218","pdf_url":null,"source":{"id":"https://openalex.org/S82722058","display_name":"Canadian Journal of Physiology and Pharmacology","issn_l":"0008-4212","issn":["0008-4212","1205-7541"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Physiology and Pharmacology","raw_type":"journal-article"},{"id":"pmid:6595051","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6595051","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian journal of physiology and pharmacology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2084634756","https://openalex.org/W1562997156","https://openalex.org/W2059859570","https://openalex.org/W2144598823","https://openalex.org/W2123215155","https://openalex.org/W2003454617","https://openalex.org/W2378837952","https://openalex.org/W1970474346","https://openalex.org/W7097594070","https://openalex.org/W1980200938"],"abstract_inverted_index":{"Previous":[0],"studies":[1],"have":[2],"shown":[3],"that":[4,39,178,201],"there":[5,43],"is":[6,34,193],"a":[7],"spontaneous":[8,140,180],"progressive":[9],"increase":[10,46,90,111,141,181],"in":[11,47,83,91,142,186],"human":[12],"chorionic":[13],"gonadotropin":[14],"(HCG)-stimulated":[15],"testosterone":[16],"(T)":[17],"production":[18,33,157,171],"by":[19,73,160,163],"immature":[20],"porcine":[21,190],"Leydig":[22,191],"cells":[23,192,214],"during":[24,40,217],"primary":[25,187],"culture":[26,126,219],"for":[27,208],"3":[28,52,69,123,183],"days,":[29],"whereas":[30,168],"basal":[31,169],"T":[32,154,170],"unaltered.":[35],"Preliminary":[36],"data":[37],"indicated":[38],"this":[41,67,139,202],"time,":[42],"was":[44,63,71,120,158,172],"an":[45],"the":[48,51,60,75,84,113,118,128,148,179,209,218],"activity":[49,92,185],"of":[50,59,77,86,112,117,125,151,182,189,212],"beta-hydroxysteroid":[53],"dehydrogenase":[54],"(3":[55],"beta-HSD).":[56],"The":[57],"purpose":[58],"present":[61],"study":[62],"to":[64,81,109,195,215],"further":[65],"characterize":[66],"increase.":[68],"beta-HSD":[70,184],"assayed":[72],"measuring":[74],"conversion":[76],"tritiated":[78],"dehydroepiandrosterone":[79],"(DHEA)":[80],"androstenedione":[82],"presence":[85],"NAD+.":[87],"A":[88,107],"ninefold":[89],"occurred":[93],"between":[94,102],"days":[95,103,124],"1":[96],"and":[97,99,105,155,165,200],"2":[98,104],"only":[100],"twofold":[101],"3.":[106],"5-":[108],"20-fold":[110],"apparent":[114,129],"maximum":[115],"velocity":[116],"enzyme":[119,143],"observed":[121],"after":[122],"while":[127],"Km":[130],"remained":[131],"unchanged.":[132],"Cycloheximide":[133],"treatment":[134,162],"(150":[135],"ng/mL)":[136],"completely":[137],"abolished":[138],"activity.":[144],"In":[145],"parallel":[146],"experiments,":[147],"stimulatory":[149],"effect":[150],"HCG":[152,216],"on":[153],"DHEA":[156],"inhibited":[159],"cycloheximide":[161],"90%":[164],"60%,":[166],"respectively,":[167],"not":[173],"affected.":[174],"These":[175],"results":[176],"suggest":[177],"cultures":[188],"secondary":[194],"de":[196],"novo":[197],"protein":[198],"synthesis":[199],"phenomenon":[203],"may":[204],"be":[205],"partially":[206],"responsible":[207],"increased":[210],"responsiveness":[211],"these":[213],"period.":[220]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}