{"id":"https://openalex.org/W2049828693","doi":"https://doi.org/10.1371/journal.pone.0071401","title":"Determination of Neuraminidase Kinetic Constants Using Whole Influenza Virus Preparations and Correction for Spectroscopic Interference by a Fluorogenic Substrate","display_name":"Determination of Neuraminidase Kinetic Constants Using Whole Influenza Virus Preparations and Correction for Spectroscopic Interference by a Fluorogenic Substrate","publication_year":2013,"publication_date":"2013-08-15","ids":{"openalex":"https://openalex.org/W2049828693","doi":"https://doi.org/10.1371/journal.pone.0071401","mag":"2049828693","pmid":"https://pubmed.ncbi.nlm.nih.gov/23977037","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3744557"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0071401","doi":"https://doi.org/10.1371/journal.pone.0071401","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0071401","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0071401&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0071401&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002367868","display_name":"Bindumadhav M. Marathe","orcid":"https://orcid.org/0000-0002-9929-7566","observed_orcids":["https://orcid.org/0000-0002-9929-7566"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bindumadhav M. Marathe","raw_affiliation_strings":["Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, United States of America","institution_ids":["https://openalex.org/I1313298211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028994393","display_name":"Vincent L\u00e9v\u00eaque","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Vincent L\u00e9v\u00eaque","raw_affiliation_strings":["Virology Discovery, Hoffmann-La Roche Inc., Nutley, New Jersey, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology Discovery, Hoffmann-La Roche Inc., Nutley, New Jersey, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076998022","display_name":"Klaus Klumpp","orcid":"https://orcid.org/0000-0002-4829-3127","observed_orcids":["https://orcid.org/0000-0002-4829-3127"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Klaus Klumpp","raw_affiliation_strings":["Virology Discovery, Hoffmann-La Roche Inc., Nutley, New Jersey, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology Discovery, Hoffmann-La Roche Inc., Nutley, New Jersey, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091454011","display_name":"Robert G. Webster","orcid":"https://orcid.org/0000-0002-1899-1856","observed_orcids":["https://orcid.org/0000-0002-1899-1856"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]},{"id":"https://openalex.org/I160606119","display_name":"University of Tennessee Health Science Center","ror":"https://ror.org/0011qv509","country_code":"US","type":"education","lineage":["https://openalex.org/I160606119"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert G. Webster","raw_affiliation_strings":["Department of Pathology, University of Tennessee, Memphis, Tennessee, United States of America","Division of Virology, Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Tennessee, Memphis, Tennessee, United States of America","institution_ids":["https://openalex.org/I160606119"]},{"raw_affiliation_string":"Division of Virology, Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, United States of America","institution_ids":["https://openalex.org/I1313298211"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067937189","display_name":"Elena A. Govorkova","orcid":"https://orcid.org/0000-0001-9067-5682","observed_orcids":["https://orcid.org/0000-0001-9067-5682"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Elena A. Govorkova","raw_affiliation_strings":["Division of Virology, Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, United States of America","institution_ids":["https://openalex.org/I1313298211"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5067937189"],"corresponding_institution_ids":["https://openalex.org/I1313298211"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":2.4104,"has_fulltext":true,"cited_by_count":69,"citation_normalized_percentile":{"value":0.88383453,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"8","issue":"8","first_page":"e71401","last_page":"e71401"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/influenza-neuraminidase","display_name":"influenza neuraminidase","score":0.9399999976158142},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.8740000128746033},{"id":"https://openalex.org/keywords/michaelis-constant","display_name":"Michaelis constant","score":0.8569999933242798},{"id":"https://openalex.org/keywords/maximum-velocity","display_name":"peak velocity","score":0.8690000176429749},{"id":"https://openalex.org/keywords/fluorogenic-substrates","display_name":"fluorogenic substrates","score":0.8500000238418579},{"id":"https://openalex.org/keywords/inner-filter-effect","display_name":"inner filter effect","score":0.8999999761581421},{"id":"https://openalex.org/keywords/sialic-acid","display_name":"sialic acid","score":0.6959999799728394},{"id":"https://openalex.org/keywords/hemagglutinin","display_name":"hemagglutinin","score":0.8220000267028809},{"id":"https://openalex.org/keywords/influenza-surveillance","display_name":"influenza surveillance","score":0.6700000166893005},{"id":"https://openalex.org/keywords/viral-fitness","display_name":"viral fitness","score":0.3440000116825104}],"concepts":[{"id":"https://openalex.org/C134164806","wikidata":"https://www.wikidata.org/wiki/Q409485","display_name":"Neuraminidase","level":3,"score":0.822537899017334},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.6886279582977295},{"id":"https://openalex.org/C2780115458","wikidata":"https://www.wikidata.org/wiki/Q419311","display_name":"Sialic acid","level":2,"score":0.6212860345840454},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5831500291824341},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5562178492546082},{"id":"https://openalex.org/C2777546802","wikidata":"https://www.wikidata.org/wiki/Q834390","display_name":"Influenza A virus","level":3,"score":0.5325462222099304},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.45468780398368835},{"id":"https://openalex.org/C2778692840","wikidata":"https://www.wikidata.org/wiki/Q9136673","display_name":"Hemagglutinin (influenza)","level":3,"score":0.45139947533607483},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4241976737976074},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3661779761314392},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3629048466682434},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.34456342458724976},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002642","descriptor_name":"Chick Embryo","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004285","descriptor_name":"Dogs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006923","descriptor_name":"Hymecromone","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D006923","descriptor_name":"Hymecromone","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009439","descriptor_name":"Neuraminidase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D053118","descriptor_name":"Influenza A Virus, H1N1 Subtype","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D057075","descriptor_name":"Enzyme Assays","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D059629","descriptor_name":"Signal-To-Noise Ratio","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D061985","descriptor_name":"Madin Darby Canine Kidney Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1371/journal.pone.0071401","doi":"https://doi.org/10.1371/journal.pone.0071401","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0071401","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0071401&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2741183","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3744557","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3744557","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3744557","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:23977037","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23977037","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:8fe59209ff6c4f4aa2cc0c8acdd7b0f6","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/8fe59209ff6c4f4aa2cc0c8acdd7b0f6","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 8, Iss 8, p e71401 (2013)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0071401","doi":"https://doi.org/10.1371/journal.pone.0071401","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0071401","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0071401&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1309367075","display_name":null,"funder_award_id":"HHSN266200700005C","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G2227505777","display_name":null,"funder_award_id":"P30CA","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G5034719483","display_name":null,"funder_award_id":"HHSN266200700005C","funder_id":"https://openalex.org/F4320313598","funder_display_name":"American Lebanese Syrian Associated Charities"},{"id":"https://openalex.org/G5663772383","display_name":null,"funder_award_id":"HHSN266200700005C","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G609259107","display_name":null,"funder_award_id":"CA21765","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G782193539","display_name":null,"funder_award_id":"21765","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G865138149","display_name":null,"funder_award_id":"CA 21765","funder_id":"https://openalex.org/F4320313598","funder_display_name":"American Lebanese Syrian Associated Charities"},{"id":"https://openalex.org/G9000086855","display_name":null,"funder_award_id":"CA21765)","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000904664","display_name":"Cancer Center Support Grant (CCSG)","funder_award_id":"P30CA021765","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320313598","display_name":"American Lebanese Syrian Associated Charities","ror":"https://ror.org/03rx10x31"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2049828693.pdf","grobid_xml":"https://content.openalex.org/works/W2049828693.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1481106099","https://openalex.org/W1560287730","https://openalex.org/W1982287510","https://openalex.org/W1982394675","https://openalex.org/W1994644330","https://openalex.org/W1999320428","https://openalex.org/W2003317860","https://openalex.org/W2016540701","https://openalex.org/W2023260449","https://openalex.org/W2026220572","https://openalex.org/W2028464664","https://openalex.org/W2036081511","https://openalex.org/W2039181792","https://openalex.org/W2046745698","https://openalex.org/W2050205112","https://openalex.org/W2053548646","https://openalex.org/W2061675984","https://openalex.org/W2104356521","https://openalex.org/W2105278533","https://openalex.org/W2107651145","https://openalex.org/W2108358599","https://openalex.org/W2111155300","https://openalex.org/W2128060816","https://openalex.org/W2129316641","https://openalex.org/W2129482297","https://openalex.org/W2133465300","https://openalex.org/W2133880910","https://openalex.org/W2138111668","https://openalex.org/W2140740881","https://openalex.org/W2145320830","https://openalex.org/W2148249666","https://openalex.org/W2154288940","https://openalex.org/W2156140162","https://openalex.org/W2164368077","https://openalex.org/W2171272529","https://openalex.org/W2313070037","https://openalex.org/W2412900389","https://openalex.org/W2412996610"],"related_works":["https://openalex.org/W2387720714","https://openalex.org/W2606220583","https://openalex.org/W4410260304","https://openalex.org/W4298087724","https://openalex.org/W2082765137","https://openalex.org/W1988876814","https://openalex.org/W2587033438","https://openalex.org/W2317930192","https://openalex.org/W4256087778","https://openalex.org/W2783589006"],"abstract_inverted_index":{"The":[0,97,194,206],"influenza":[1,56,81,110],"neuraminidase":[2],"(NA)":[3],"enzyme":[4,119],"cleaves":[5],"terminal":[6],"sialic":[7,29],"acid":[8,30,103],"residues":[9],"from":[10],"cellular":[11],"receptors,":[12],"a":[13,197],"process":[14],"required":[15],"for":[16,38,66,86,152,180],"the":[17,52,67,87,142,153,172],"release":[18],"of":[19,25,33,55,69,72,89,93,99,117,141,145,158,168,191,200,214,221],"newly":[20],"synthesized":[21],"virions.":[22],"A":[23,61],"balance":[24],"NA":[26,42,73,118,215],"activity":[27,74,120],"with":[28,174,202],"binding":[31],"affinity":[32],"hemagglutinin":[34],"(HA)":[35],"is":[36],"important":[37,124],"optimal":[39],"virus":[40,57,95,111],"replication.":[41],"sequence":[43],"evolution":[44],"through":[45],"genetic":[46],"shift":[47],"and":[48,59,63,83,91,105,132,147,183,218],"drift":[49],"contributes":[50],"to":[51,79,126,161],"continuous":[53],"modulation":[54],"fitness":[58,90],"pathogenicity.":[60],"simple":[62],"reliable":[64],"method":[65],"determination":[68,140],"kinetic":[70,134,216],"parameters":[71,85,135,217],"could":[75],"add":[76],"significant":[77,198],"value":[78],"global":[80],"surveillance":[82],"provide":[84],"projection":[88],"pathogenicity":[92],"emerging":[94],"variants.":[96],"use":[98],"fluorogenic":[100],"substrate":[101,169],"2'-(4-methylumbelliferyl)-\u03b1-D-N-acetylneuraminic":[102],"(MUNANA)":[104],"cell-":[106],"or":[107],"egg-grown":[108],"whole":[109],"preparations":[112],"have":[113],"been":[114],"attractive":[115],"components":[116],"investigations.":[121],"We":[122],"describe":[123],"criteria":[125,207],"be":[127],"addressed":[128],"when":[129],"determining":[130],"K(m)":[131],"V(max)":[133],"using":[136],"this":[137],"method:":[138],"(1)":[139],"dynamic":[143],"range":[144],"MUNANA":[146,192,201],"4-methylumbelliferone":[148],"product":[149],"(4-MU)":[150],"fluorescence":[151,204],"instrument":[154],"used;":[155],"(2)":[156],"adjustment":[157],"reaction":[159],"conditions":[160],"approximate":[162],"initial":[163],"rate":[164],"conditions,":[165],"i.e.":[166],"\u226415%":[167],"converted":[170],"during":[171],"reaction,":[173],"signal-to-noise":[175],"ratio":[176],"\u226510;":[177],"(3)":[178],"correction":[179],"optical":[181],"interference":[182,199],"inner":[184],"filter":[185],"effect":[186],"caused":[187],"by":[188],"increasing":[189],"concentrations":[190],"substrate.":[193],"results":[195],"indicate":[196],"4-MU":[203],"determination.":[205],"proposed":[208],"enable":[209],"an":[210],"improved":[211],"rapid":[212],"estimation":[213],"facilitate":[219],"comparison":[220],"data":[222],"between":[223],"laboratories.":[224]},"counts_by_year":[{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":14},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}