{"id":"https://openalex.org/W2049219115","doi":"https://doi.org/10.1021/pr8000749","title":"Scaled-Down Purification Protocol To Access Proteomic Analysis of 20S Proteasome from Human Tissue Samples: Comparison of Normal and Tumor Colorectal Cells","display_name":"Scaled-Down Purification Protocol To Access Proteomic Analysis of 20S Proteasome from Human Tissue Samples: Comparison of Normal and Tumor Colorectal Cells","publication_year":2008,"publication_date":"2008-05-30","ids":{"openalex":"https://openalex.org/W2049219115","doi":"https://doi.org/10.1021/pr8000749","mag":"2049219115","pmid":"https://pubmed.ncbi.nlm.nih.gov/18510353"},"language":"en","primary_location":{"id":"doi:10.1021/pr8000749","doi":"https://doi.org/10.1021/pr8000749","is_oa":false,"landing_page_url":"https://doi.org/10.1021/pr8000749","pdf_url":null,"source":{"id":"https://openalex.org/S74367812","display_name":"Journal of Proteome Research","issn_l":"1535-3893","issn":["1535-3893","1535-3907"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Proteome Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065608015","display_name":"Manuelle Ducoux\u2010Petit","orcid":"https://orcid.org/0000-0002-5346-2044","observed_orcids":["https://orcid.org/0000-0002-5346-2044"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Manuelle Ducoux-Petit","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037775438","display_name":"Sandrine Uttenweiler\u2010Joseph","orcid":"https://orcid.org/0000-0001-9019-4766","observed_orcids":["https://orcid.org/0000-0001-9019-4766"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Sandrine Uttenweiler-Joseph","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112198750","display_name":"Franck Brichory","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Franck Brichory","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010499759","display_name":"Marie\u2010Pierre Bousquet","orcid":"https://orcid.org/0000-0003-2680-8932","observed_orcids":["https://orcid.org/0000-0003-2680-8932"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Marie-Pierre Bousquet-Dubouch","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069577241","display_name":"Odile Burlet\u2010Schiltz","orcid":"https://orcid.org/0000-0002-3606-2356","observed_orcids":["https://orcid.org/0000-0002-3606-2356"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Odile Burlet-Schiltz","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108220952","display_name":"Jean\u2010Fran\u00e7ois Haeuw","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jean-Fran\u00e7ois Haeuw","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5064172203","display_name":"Bernard Monsarrat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210099749","display_name":"Institut de Pharmacologie et de Biologie Structurale","ror":"https://ror.org/016zvc994","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210099749","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Bernard Monsarrat","raw_affiliation_strings":["Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Toulouse, Institute of Pharmacology and Structural Biology, IPBS, UPS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, CNRS, IPBS, 205 route de Narbonne, 31077, Toulouse, cedex 4, France, and Centre d\u2019Immunologie Pierre Fabre, 5 avenue Napol\u00e9on III, BP 497, 74164 Saint Julien en Genevois Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5037775438"],"corresponding_institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I134560555","https://openalex.org/I4210099749","https://openalex.org/I4210135355","https://openalex.org/I4405258862"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.12,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.74336629,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"7","issue":"7","first_page":"2852","last_page":"2859"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/20s-proteasome","display_name":"20S proteasome","score":1.0},{"id":"https://openalex.org/keywords/proteasome-purification","display_name":"proteasome purification","score":0.9120000004768372},{"id":"https://openalex.org/keywords/magnetic-beads","display_name":"magnetic beads","score":0.9139999747276306},{"id":"https://openalex.org/keywords/colorectal-cancer","display_name":"colorectal cancer","score":0.9359999895095825},{"id":"https://openalex.org/keywords/proteasome-subunit-composition","display_name":"proteasome subunit composition","score":0.9340000152587891},{"id":"https://openalex.org/keywords/proteasome-activity","display_name":"proteasome activity","score":0.9929999709129333},{"id":"https://openalex.org/keywords/structure-activity-relationship","display_name":"structure-activity relationship","score":0.6909999847412109},{"id":"https://openalex.org/keywords/human-tissue-samples","display_name":"human tissue samples","score":0.5609999895095825},{"id":"https://openalex.org/keywords/proteomics","display_name":"proteomics","score":0.5730000138282776}],"concepts":[{"id":"https://openalex.org/C27740335","wikidata":"https://www.wikidata.org/wiki/Q14878333","display_name":"Proteasome","level":2,"score":0.9028910994529724},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.5504093766212463},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4900780916213989},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.47234785556793213},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.4385395646095276},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.4334373474121094},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4320669174194336},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.42657771706581116},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4042421579360962},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3220450282096863},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19036439061164856},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12687209248542786}],"mesh":[{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000369","descriptor_name":"Aged, 80 and over","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008280","descriptor_name":"Magnetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015179","descriptor_name":"Colorectal Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D015179","descriptor_name":"Colorectal Neoplasms","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046988","descriptor_name":"Proteasome Endopeptidase Complex","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D046988","descriptor_name":"Proteasome Endopeptidase Complex","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D047468","descriptor_name":"Immunoprecipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/pr8000749","doi":"https://doi.org/10.1021/pr8000749","is_oa":false,"landing_page_url":"https://doi.org/10.1021/pr8000749","pdf_url":null,"source":{"id":"https://openalex.org/S74367812","display_name":"Journal of Proteome Research","issn_l":"1535-3893","issn":["1535-3893","1535-3907"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Proteome Research","raw_type":"journal-article"},{"id":"pmid:18510353","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18510353","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of proteome research","raw_type":"Comparative Study"},{"id":"pmh:oai:HAL:hal-02504491v1","doi":null,"is_oa":false,"landing_page_url":"https://insa-toulouse.hal.science/hal-02504491","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Proteome Research, 2008, 7 (7), pp.2852-2859. ⟨10.1021/pr8000749⟩","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.766700029373169,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W53803839","https://openalex.org/W1556426431","https://openalex.org/W1603044497","https://openalex.org/W1963689482","https://openalex.org/W1976582166","https://openalex.org/W1982107660","https://openalex.org/W1983403197","https://openalex.org/W1988746802","https://openalex.org/W1999701266","https://openalex.org/W2005317701","https://openalex.org/W2025714022","https://openalex.org/W2025890297","https://openalex.org/W2028699526","https://openalex.org/W2033715459","https://openalex.org/W2040863926","https://openalex.org/W2057832589","https://openalex.org/W2065617511","https://openalex.org/W2069723920","https://openalex.org/W2070434454","https://openalex.org/W2076711139","https://openalex.org/W2079504029","https://openalex.org/W2092725115","https://openalex.org/W2095331480","https://openalex.org/W2105469941","https://openalex.org/W2118937204","https://openalex.org/W2128403792","https://openalex.org/W2131502197","https://openalex.org/W2136735139","https://openalex.org/W2148173928","https://openalex.org/W2150123812","https://openalex.org/W2150632173","https://openalex.org/W2158930367","https://openalex.org/W2159887483","https://openalex.org/W2182979694","https://openalex.org/W2400251154","https://openalex.org/W2414223325","https://openalex.org/W3159164887","https://openalex.org/W4243155738"],"related_works":["https://openalex.org/W2062003845","https://openalex.org/W1979286486","https://openalex.org/W53803839","https://openalex.org/W2427882327","https://openalex.org/W4213178320","https://openalex.org/W1603044497","https://openalex.org/W2088084798","https://openalex.org/W1982297162","https://openalex.org/W1726555330","https://openalex.org/W1993908102"],"abstract_inverted_index":{"The":[0,122,146],"proteasome":[1,38,78,123,149],"is":[2,164],"a":[3,60],"proteolytic":[4],"complex":[5],"that":[6,43,105],"constitutes":[7],"the":[8,36,55,75,106,129,142],"main":[9],"pathway":[10],"for":[11,54],"degradation":[12],"of":[13,57,63,79,90,109],"intracellular":[14],"proteins":[15],"in":[16,41,128,137,141],"eukaryotic":[17],"cells.":[18,145],"It":[19],"regulates":[20],"many":[21],"physiological":[22],"processes":[23],"and":[24,82,101,115,132,163],"its":[25],"dysfunction":[26],"can":[27,154],"lead":[28],"to":[29,73,157],"several":[30,91],"pathologies":[31],"like":[32],"cancer.":[33],"To":[34],"study":[35],"20S":[37,77,110,148],"structure/activity":[39],"relationship":[40],"cells":[42,64,85,117],"derive":[44],"from":[45,87,112],"human":[46,159],"biopsy":[47],"samples,":[48],"we":[49],"optimized":[50],"an":[51],"immuno-purification":[52],"protocol":[53,70,151],"analysis":[56],"samples":[58,89,162],"containing":[59],"small":[61],"number":[62],"using":[65],"magnetic":[66],"beads.":[67],"This":[68],"scaled-down":[69,147],"was":[71,125,133],"used":[72],"purify":[74],"cytoplasmic":[76,130],"adjacent":[80],"normal":[81,114,144],"tumor":[83,116,138],"colorectal":[84,139],"arising":[86],"tissue":[88,161],"patients.":[92],"Proteomic":[93],"analyses":[94],"based":[95],"on":[96],"two-dimensional":[97],"gel":[98],"electrophoresis":[99],"(2DE)":[100],"mass":[102],"spectrometry":[103],"showed":[104],"subunit":[107],"composition":[108],"proteasomes":[111],"these":[113],"were":[118],"not":[119],"significantly":[120],"different.":[121],"activity":[124],"also":[126],"assessed":[127],"extracts":[131],"similar":[134],"or":[135],"higher":[136],"than":[140],"corresponding":[143],"purification":[150],"developed":[152],"here":[153],"be":[155],"applied":[156],"any":[158],"clinical":[160],"compatible":[165],"with":[166],"further":[167],"proteomic":[168],"analyses.":[169]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}