{"id":"https://openalex.org/W2048640865","doi":"https://doi.org/10.1590/s1516-89132008000700020","title":"Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli","display_name":"Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli","publication_year":2008,"publication_date":"2008-12-01","ids":{"openalex":"https://openalex.org/W2048640865","doi":"https://doi.org/10.1590/s1516-89132008000700020","mag":"2048640865"},"language":"en","primary_location":{"id":"doi:10.1590/s1516-89132008000700020","doi":"https://doi.org/10.1590/s1516-89132008000700020","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s1516-89132008000700020","pdf_url":"https://www.scielo.br/j/babt/a/5jPXxMZHrxc4SDpY84SX5Sb/?lang=en&format=pdf","source":{"id":"https://openalex.org/S175992573","display_name":"Brazilian Archives of Biology and Technology","issn_l":"1516-8913","issn":["1516-8913","1678-4324"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-40","russia-white-list-2","scielo"],"host_organization":"https://openalex.org/P4310312018","host_organization_name":"Instituto de Tecnologia do Paran\u00e1 (Tecpar)","host_organization_lineage":["https://openalex.org/P4310312018"],"host_organization_lineage_names":["Instituto de Tecnologia do Paran\u00e1 (Tecpar)"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Brazilian Archives of Biology and Technology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.scielo.br/j/babt/a/5jPXxMZHrxc4SDpY84SX5Sb/?lang=en&format=pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003977231","display_name":"Jos\u00e9 Carlos Pelielo de Mattos","orcid":"https://orcid.org/0000-0002-4923-4356","observed_orcids":["https://orcid.org/0000-0002-4923-4356"]},"institutions":[{"id":"https://openalex.org/I40034438","display_name":"Universidade do Estado do Rio de Janeiro","ror":"https://ror.org/0198v2949","country_code":"BR","type":"education","lineage":["https://openalex.org/I40034438"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Jos\u00e9 Carlos Pelielo de Mattos","raw_affiliation_strings":["Universidade do estado do Rio de Janeiro, Brazil","Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade do estado do Rio de Janeiro, Brazil","institution_ids":["https://openalex.org/I40034438"]},{"raw_affiliation_string":"Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil","institution_ids":["https://openalex.org/I40034438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029237609","display_name":"Ellen Serri da Motta","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40034438","display_name":"Universidade do Estado do Rio de Janeiro","ror":"https://ror.org/0198v2949","country_code":"BR","type":"education","lineage":["https://openalex.org/I40034438"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Ellen Serri da Motta","raw_affiliation_strings":["Universidade do estado do Rio de Janeiro, Brazil","Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade do estado do Rio de Janeiro, Brazil","institution_ids":["https://openalex.org/I40034438"]},{"raw_affiliation_string":"Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil","institution_ids":["https://openalex.org/I40034438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020745794","display_name":"M. B.N. Oliveira","orcid":"https://orcid.org/0000-0001-7672-6121","observed_orcids":["https://orcid.org/0000-0001-7672-6121"]},"institutions":[{"id":"https://openalex.org/I40034438","display_name":"Universidade do Estado do Rio de Janeiro","ror":"https://ror.org/0198v2949","country_code":"BR","type":"education","lineage":["https://openalex.org/I40034438"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"M\u00e1rcia Betania Nunes de Oliveira","raw_affiliation_strings":["Universidade do estado do Rio de Janeiro, Brazil","Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade do estado do Rio de Janeiro, Brazil","institution_ids":["https://openalex.org/I40034438"]},{"raw_affiliation_string":"Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil","institution_ids":["https://openalex.org/I40034438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070444300","display_name":"Fl\u00e1vio Jos\u00e9 da Silva Dantas","orcid":"https://orcid.org/0000-0001-6243-7169","observed_orcids":["https://orcid.org/0000-0001-6243-7169"]},"institutions":[{"id":"https://openalex.org/I40034438","display_name":"Universidade do Estado do Rio de Janeiro","ror":"https://ror.org/0198v2949","country_code":"BR","type":"education","lineage":["https://openalex.org/I40034438"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Fl\u00e1vio Jos\u00e9 da Silva Dantas","raw_affiliation_strings":["Universidade do estado do Rio de Janeiro, Brazil","Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade do estado do Rio de Janeiro, Brazil","institution_ids":["https://openalex.org/I40034438"]},{"raw_affiliation_string":"Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil","institution_ids":["https://openalex.org/I40034438"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103479635","display_name":"Adriano Caldeira de Ara\u00fajo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40034438","display_name":"Universidade do Estado do Rio de Janeiro","ror":"https://ror.org/0198v2949","country_code":"BR","type":"education","lineage":["https://openalex.org/I40034438"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Adriano Caldeira de Araujo","raw_affiliation_strings":["Universidade do estado do Rio de Janeiro, Brazil","Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade do estado do Rio de Janeiro, Brazil","institution_ids":["https://openalex.org/I40034438"]},{"raw_affiliation_string":"Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil","institution_ids":["https://openalex.org/I40034438"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I40034438"],"apc_list":null,"apc_paid":null,"fwci":0.224,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.50167674,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"51","issue":"spe","first_page":"121","last_page":"126"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.5679000020027161,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.5679000020027161,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.4113999903202057,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.010400000028312206,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-strand-breaks","display_name":"DNA strand breaks","score":0.9399999976158142},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.7350000143051147},{"id":"https://openalex.org/keywords/base-excision-repair","display_name":"base excision repair","score":0.9760000109672546},{"id":"https://openalex.org/keywords/tin-ii-chloride","display_name":"tin(II) chloride","score":0.8299999833106995},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.9800000190734863},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9240000247955322},{"id":"https://openalex.org/keywords/endonuclease-iv","display_name":"endonuclease IV","score":0.925000011920929},{"id":"https://openalex.org/keywords/exonuclease-iii","display_name":"exonuclease III","score":1.0},{"id":"https://openalex.org/keywords/genotoxicity-testing","display_name":"genotoxicity testing","score":0.6809999942779541}],"concepts":[{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.7035418748855591},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.6970376968383789},{"id":"https://openalex.org/C2781243718","wikidata":"https://www.wikidata.org/wiki/Q5420625","display_name":"Exonuclease III","level":4,"score":0.6606202721595764},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6565238833427429},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.6438558101654053},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5458652377128601},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5380733609199524},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.5203728079795837},{"id":"https://openalex.org/C2780358455","wikidata":"https://www.wikidata.org/wiki/Q903780","display_name":"Comet assay","level":4,"score":0.5173022747039795},{"id":"https://openalex.org/C2778959862","wikidata":"https://www.wikidata.org/wiki/Q1009245","display_name":"Genotoxicity","level":3,"score":0.48802128434181213},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.45823389291763306},{"id":"https://openalex.org/C187206112","wikidata":"https://www.wikidata.org/wiki/Q3113091","display_name":"Base excision repair","level":4,"score":0.4390898048877716},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40998417139053345},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2685283422470093},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11319530010223389},{"id":"https://openalex.org/C29730261","wikidata":"https://www.wikidata.org/wiki/Q274160","display_name":"Toxicity","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1590/s1516-89132008000700020","doi":"https://doi.org/10.1590/s1516-89132008000700020","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s1516-89132008000700020","pdf_url":"https://www.scielo.br/j/babt/a/5jPXxMZHrxc4SDpY84SX5Sb/?lang=en&format=pdf","source":{"id":"https://openalex.org/S175992573","display_name":"Brazilian Archives of Biology and Technology","issn_l":"1516-8913","issn":["1516-8913","1678-4324"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-40","russia-white-list-2","scielo"],"host_organization":"https://openalex.org/P4310312018","host_organization_name":"Instituto de Tecnologia do Paran\u00e1 (Tecpar)","host_organization_lineage":["https://openalex.org/P4310312018"],"host_organization_lineage_names":["Instituto de Tecnologia do Paran\u00e1 (Tecpar)"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Brazilian Archives of Biology and Technology","raw_type":"journal-article"},{"id":"pmh:oai:scielo:S1516-89132008000700020","doi":null,"is_oa":true,"landing_page_url":"http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Brazilian Archives of Biology and Technology v.51 n.spe 2008","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:americanae.aecid.es:2443205","doi":null,"is_oa":true,"landing_page_url":"http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020","pdf_url":null,"source":{"id":"https://openalex.org/S4306400786","display_name":"Americanae (AECID Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Brazilian Archives of Biology and Technology v.51 n.spe 2008","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:ba960a45f1824088a7232bf72141ba07","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/ba960a45f1824088a7232bf72141ba07","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Brazilian Archives of Biology and Technology, Vol 51, Iss spe, Pp 121-126 (2008)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1590/s1516-89132008000700020","doi":"https://doi.org/10.1590/s1516-89132008000700020","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s1516-89132008000700020","pdf_url":"https://www.scielo.br/j/babt/a/5jPXxMZHrxc4SDpY84SX5Sb/?lang=en&format=pdf","source":{"id":"https://openalex.org/S175992573","display_name":"Brazilian Archives of Biology and Technology","issn_l":"1516-8913","issn":["1516-8913","1678-4324"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-40","russia-white-list-2","scielo"],"host_organization":"https://openalex.org/P4310312018","host_organization_name":"Instituto de Tecnologia do Paran\u00e1 (Tecpar)","host_organization_lineage":["https://openalex.org/P4310312018"],"host_organization_lineage_names":["Instituto de Tecnologia do Paran\u00e1 (Tecpar)"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Brazilian Archives of Biology and Technology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1840770272","display_name":null,"funder_award_id":"E-26/","funder_id":"https://openalex.org/F4320322749","funder_display_name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro"}],"funders":[{"id":"https://openalex.org/F4320322025","display_name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","ror":"https://ror.org/03swz6y49"},{"id":"https://openalex.org/F4320322749","display_name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","ror":"https://ror.org/03kk0s825"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2048640865.pdf","grobid_xml":"https://content.openalex.org/works/W2048640865.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W1491876634","https://openalex.org/W1546472725","https://openalex.org/W1579892113","https://openalex.org/W1780014751","https://openalex.org/W1963712961","https://openalex.org/W1975127082","https://openalex.org/W1977913875","https://openalex.org/W1978870715","https://openalex.org/W2017564717","https://openalex.org/W2025528877","https://openalex.org/W2029130914","https://openalex.org/W2052178794","https://openalex.org/W2054970021","https://openalex.org/W2064816788","https://openalex.org/W2092629964","https://openalex.org/W2092998226","https://openalex.org/W2126473492","https://openalex.org/W2126902508","https://openalex.org/W2140126848","https://openalex.org/W2147762625","https://openalex.org/W4299546695"],"related_works":["https://openalex.org/W2064816788","https://openalex.org/W7111470140","https://openalex.org/W7096817180","https://openalex.org/W2073185020","https://openalex.org/W2593560261","https://openalex.org/W2309549135","https://openalex.org/W180623734","https://openalex.org/W2395437000","https://openalex.org/W2017564717","https://openalex.org/W7061047628"],"abstract_inverted_index":{"Reactive":[0],"oxygen":[1],"species":[2],"(ROS)":[3],"can":[4],"induce":[5,104],"lesions":[6],"in":[7,23,38,52,84,108,121],"different":[8],"cellular":[9],"targets,":[10],"including":[11],"DNA.":[12],"Stannous":[13],"chloride":[14],"(SnCl2)":[15],"is":[16],"a":[17,36,119],"ROS":[18],"generator,":[19],"leading":[20],"to":[21,46,75,103],"lethality":[22],"Escherichia":[24],"coli":[25,89],"(E.":[26],"coli),":[27],"with":[28],"the":[29,67,77,125,131],"base":[30],"excision":[31],"repair":[32,94,149],"(BER)":[33],"mechanism":[34],"playing":[35],"role":[37,120],"this":[39,62],"process.":[40],"Many":[41],"techniques":[42],"have":[43],"been":[44],"developed":[45],"detect":[47],"genotoxicity,":[48],"as":[49],"comet":[50],"assay,":[51],"eukaryotic":[53],"cells,":[54],"and":[55,92,115,146],"plasmid":[56],"DNA":[57,105,122,142],"agarose":[58],"gel":[59,69,133],"electrophoresis.":[60],"In":[61],"study,":[63],"an":[64],"adaptation":[65],"of":[66,79],"alkaline":[68,132],"electrophoresis":[70,134],"method":[71],"was":[72,101],"carried":[73],"out":[74],"ascertain":[76],"induction":[78,145],"strand":[80,106,143],"breaks":[81,107,144],"by":[82],"SnCl2":[83,100],"bacterial":[85],"DNA,":[86],"from":[87],"E.":[88],"BER":[90],"mutants,":[91],"its":[93],"pathway.":[95],"Results":[96],"obtained":[97],"show":[98],"that":[99,130],"able":[102],"all":[109],"strains":[110],"tested.":[111],"Moreover,":[112],"endonuclease":[113],"IV":[114],"exonuclease":[116],"III":[117],"play":[118],"repair.":[123],"On":[124],"whole,":[126],"data":[127],"has":[128],"shown":[129],"assay":[135],"could":[136],"be":[137],"used":[138],"both":[139],"for":[140,147],"studying":[141],"associated":[148],"mechanisms.":[150]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2016-06-24T00:00:00"}