{"id":"https://openalex.org/W2048397996","doi":"https://doi.org/10.1007/s13361-014-0946-4","title":"Collision-Induced Release, Ion Mobility Separation, and Amino Acid Sequence Analysis of Subunits from Mass-Selected Noncovalent Protein Complexes","display_name":"Collision-Induced Release, Ion Mobility Separation, and Amino Acid Sequence Analysis of Subunits from Mass-Selected Noncovalent Protein Complexes","publication_year":2014,"publication_date":"2014-07-07","ids":{"openalex":"https://openalex.org/W2048397996","doi":"https://doi.org/10.1007/s13361-014-0946-4","mag":"2048397996","pmid":"https://pubmed.ncbi.nlm.nih.gov/25001382"},"language":"en","primary_location":{"id":"doi:10.1007/s13361-014-0946-4","doi":"https://doi.org/10.1007/s13361-014-0946-4","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s13361-014-0946-4","pdf_url":null,"source":{"id":"https://openalex.org/S60743949","display_name":"Journal of the American Society for Mass Spectrometry","issn_l":"1044-0305","issn":["1044-0305","1879-1123"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079415198","display_name":"Deepali Rathore","orcid":"https://orcid.org/0000-0002-3157-7000","observed_orcids":["https://orcid.org/0000-0002-3157-7000"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Deepali Rathore","raw_affiliation_strings":["Department of Chemistry, University of Nebraska-Lincoln, 68588-0304, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Nebraska-Lincoln, 68588-0304, Lincoln, NE, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072075494","display_name":"Eric D. Dodds","orcid":"https://orcid.org/0000-0002-7721-394X","observed_orcids":["https://orcid.org/0000-0002-7721-394X"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric D. Dodds","raw_affiliation_strings":["Department of Chemistry, University of Nebraska-Lincoln, 68588-0304, Lincoln, NE, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Nebraska-Lincoln, 68588-0304, Lincoln, NE, USA","institution_ids":["https://openalex.org/I114395901"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I114395901"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.8562,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.66791093,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"25","issue":"9","first_page":"1600","last_page":"1609"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/noncovalent-protein-complexes","display_name":"noncovalent protein complexes","score":0.9390000104904175},{"id":"https://openalex.org/keywords/collision-induced-dissociation","display_name":"collision-induced dissociation","score":0.8109999895095825},{"id":"https://openalex.org/keywords/ion-mobility-separation","display_name":"ion mobility separation","score":0.9789999723434448},{"id":"https://openalex.org/keywords/traveling-wave-ion-mobility","display_name":"traveling wave ion mobility","score":0.8709999918937683},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.7820000052452087},{"id":"https://openalex.org/keywords/homodimer","display_name":"homodimer","score":0.6610000133514404},{"id":"https://openalex.org/keywords/collision-cross-sections","display_name":"collision cross sections","score":0.6690000295639038},{"id":"https://openalex.org/keywords/subunit-stoichiometry","display_name":"subunit stoichiometry","score":0.7680000066757202},{"id":"https://openalex.org/keywords/glucagon","display_name":"glucagon","score":0.6230000257492065},{"id":"https://openalex.org/keywords/ubiquitin","display_name":"ubiquitin","score":1.0},{"id":"https://openalex.org/keywords/cytochrome-c","display_name":"cytochrome c","score":1.0},{"id":"https://openalex.org/keywords/beta-lactoglobulin","display_name":"\u03b2-lactoglobulin","score":0.9120000004768372},{"id":"https://openalex.org/keywords/tandem-mass-spectrometry","display_name":"tandem mass spectrometry","score":0.875}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8530870676040649},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.7249063849449158},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6844817399978638},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.6315872073173523},{"id":"https://openalex.org/C102931765","wikidata":"https://www.wikidata.org/wiki/Q189673","display_name":"Dissociation (chemistry)","level":2,"score":0.6002817153930664},{"id":"https://openalex.org/C176107965","wikidata":"https://www.wikidata.org/wiki/Q3710336","display_name":"Collision-induced dissociation","level":4,"score":0.5359823107719421},{"id":"https://openalex.org/C15213710","wikidata":"https://www.wikidata.org/wiki/Q904207","display_name":"Ion-mobility spectrometry","level":3,"score":0.5322774648666382},{"id":"https://openalex.org/C137277065","wikidata":"https://www.wikidata.org/wiki/Q2915446","display_name":"Non-covalent interactions","level":4,"score":0.4110090732574463},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.4032946527004242},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.34885719418525696},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3378722667694092},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2152528166770935},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.20758062601089478},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.11825397610664368},{"id":"https://openalex.org/C112887158","wikidata":"https://www.wikidata.org/wiki/Q169324","display_name":"Hydrogen bond","level":3,"score":0.10779604315757751},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005934","descriptor_name":"Glucagon","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007782","descriptor_name":"Lactoglobulins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D020539","descriptor_name":"Sequence Analysis, Protein","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D025801","descriptor_name":"Ubiquitin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D045304","descriptor_name":"Cytochromes c","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D055503","descriptor_name":"Protein Multimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s13361-014-0946-4","doi":"https://doi.org/10.1007/s13361-014-0946-4","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s13361-014-0946-4","pdf_url":null,"source":{"id":"https://openalex.org/S60743949","display_name":"Journal of the American Society for Mass Spectrometry","issn_l":"1044-0305","issn":["1044-0305","1879-1123"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:25001382","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25001382","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320316613","display_name":"Nebraska Tobacco Settlement Biomedical Research Development Fund","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W1965817736","https://openalex.org/W1971800073","https://openalex.org/W1987505759","https://openalex.org/W1988332348","https://openalex.org/W1991998914","https://openalex.org/W1997645853","https://openalex.org/W2000798961","https://openalex.org/W2004531406","https://openalex.org/W2004645109","https://openalex.org/W2008876483","https://openalex.org/W2011859588","https://openalex.org/W2011887852","https://openalex.org/W2012801290","https://openalex.org/W2019812098","https://openalex.org/W2021199425","https://openalex.org/W2027138806","https://openalex.org/W2030265676","https://openalex.org/W2034489451","https://openalex.org/W2035546135","https://openalex.org/W2038563588","https://openalex.org/W2041516405","https://openalex.org/W2041603270","https://openalex.org/W2045907498","https://openalex.org/W2050060187","https://openalex.org/W2057424991","https://openalex.org/W2058794436","https://openalex.org/W2065001285","https://openalex.org/W2068508879","https://openalex.org/W2068722030","https://openalex.org/W2074901150","https://openalex.org/W2075889405","https://openalex.org/W2078811015","https://openalex.org/W2081961557","https://openalex.org/W2083486056","https://openalex.org/W2086145691","https://openalex.org/W2092462626","https://openalex.org/W2097328374","https://openalex.org/W2111527039","https://openalex.org/W2115569106","https://openalex.org/W2129733824","https://openalex.org/W2147774349","https://openalex.org/W2147990271","https://openalex.org/W2156467165","https://openalex.org/W2166127438","https://openalex.org/W2325556600","https://openalex.org/W2330891955","https://openalex.org/W2335117233"],"related_works":["https://openalex.org/W1987505759","https://openalex.org/W2085559869","https://openalex.org/W2158998104","https://openalex.org/W2114871642","https://openalex.org/W2315485810","https://openalex.org/W2074901150","https://openalex.org/W2109486727","https://openalex.org/W1254760643","https://openalex.org/W2055106056","https://openalex.org/W1973167628"],"abstract_inverted_index":{"In":[0,157],"recent":[1],"years,":[2],"mass":[3,37,100],"spectrometry":[4,38],"has":[5],"become":[6],"a":[7,71,83,87,97,104,114,219,227],"valuable":[8],"tool":[9],"for":[10,17,70,113,215],"detecting":[11],"and":[12,16,21,46,155,161,212,242],"characterizing":[13],"protein-protein":[14],"interactions":[15],"measuring":[18,199],"the":[19,172,184,195,216,231],"masses":[20,45],"subunit":[22,44,56,75,206,208,245],"stoichiometries":[23],"of":[24,31,68,128,135,171,186,198,233],"noncovalent":[25,32,115],"protein":[26,33,84,116,234],"complexes.":[27],"The":[28,91],"gas-phase":[29,89,221],"dissociation":[30,49,130],"assemblies":[34],"via":[35],"tandem":[36],"can":[39],"be":[40,61,79,119],"useful":[41],"in":[42,86,176,218],"confirming":[43],"stoichiometries;":[47],"however,":[48],"experiments":[50,92],"that":[51,73],"are":[52],"able":[53],"to":[54,78,118,124,143,178,183],"yield":[55,244],"sequence":[57,76,180,213],"information":[58,77,214],"must":[59],"usually":[60],"conducted":[62],"separately.":[63],"Here,":[64],"we":[65],"furnish":[66],"proof":[67],"concept":[69],"method":[72],"allows":[74,112],"directly":[80,243],"obtained":[81,166],"from":[82],"aggregate":[85],"single":[88,220],"analysis.":[90],"were":[93,165],"carried":[94],"out":[95],"using":[96],"quadrupole":[98,120],"time-of-flight":[99],"spectrometer":[101],"equipped":[102],"with":[103,131,239],"traveling-wave":[105],"ion":[106,136,188],"mobility":[107,137,189],"separator.":[108],"This":[109,139],"instrument":[110],"configuration":[111],"assembly":[117],"selected,":[121],"then":[122],"subjected":[123],"two":[125],"successive":[126],"rounds":[127],"collision-induced":[129],"an":[132,187],"intervening":[133],"stage":[134],"separation.":[138],"approach":[140],"was":[141],"applied":[142],"four":[144],"model":[145],"proteins":[146],"as":[147],"their":[148],"corresponding":[149],"homodimers:":[150],"glucagon,":[151],"ubiquitin,":[152],"cytochrome":[153],"c,":[154],"\u03b2-lactoglobulin.":[156],"each":[158],"case,":[159],"b-":[160],"y-type":[162],"fragment":[163],"ions":[164],"upon":[167],"further":[168],"collisional":[169,203,209],"activation":[170],"collisionally-released":[173],"subunits,":[174],"resulting":[175],"up":[177],"50%":[179],"coverage.":[181],"Owing":[182],"incorporation":[185],"separation,":[190],"these":[191,224],"results":[192],"also":[193],"suggest":[194],"intriguing":[196],"possibility":[197],"complex":[200,202],"mass,":[201],"cross":[204,210],"section,":[205],"masses,":[207],"sections,":[211],"subunits":[217],"experiment.":[222],"Overall,":[223],"findings":[225],"represent":[226],"significant":[228],"contribution":[229],"towards":[230],"realization":[232],"interactomic":[235],"analyses,":[236],"which":[237],"begin":[238],"native":[240],"complexes":[241],"identities.":[246]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}