{"id":"https://openalex.org/W2048104591","doi":"https://doi.org/10.1016/j.femsle.2004.08.046","title":"Cloning and functional analysis by gene disruption of a novel gene involved in indigo production and fluoranthene metabolism inPseudomonas alcaligenesPA-10","display_name":"Cloning and functional analysis by gene disruption of a novel gene involved in indigo production and fluoranthene metabolism inPseudomonas alcaligenesPA-10","publication_year":2004,"publication_date":"2004-09-14","ids":{"openalex":"https://openalex.org/W2048104591","doi":"https://doi.org/10.1016/j.femsle.2004.08.046","mag":"2048104591","pmid":"https://pubmed.ncbi.nlm.nih.gov/15476978"},"language":"en","primary_location":{"id":"doi:10.1016/j.femsle.2004.08.046","doi":"https://doi.org/10.1016/j.femsle.2004.08.046","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.femsle.2004.08.046","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003354815","display_name":"Debebe Alemayehu","orcid":"https://orcid.org/0000-0003-3151-308X","observed_orcids":["https://orcid.org/0000-0003-3151-308X"]},"institutions":[{"id":"https://openalex.org/I181231927","display_name":"National University of Ireland","ror":"https://ror.org/00shsf120","country_code":"IE","type":"education","lineage":["https://openalex.org/I181231927"]},{"id":"https://openalex.org/I27577105","display_name":"University College Cork","ror":"https://ror.org/03265fv13","country_code":"IE","type":"education","lineage":["https://openalex.org/I27577105"]}],"countries":["IE"],"is_corresponding":false,"raw_author_name":"D. Alemayehu","raw_affiliation_strings":["Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland","institution_ids":["https://openalex.org/I181231927","https://openalex.org/I27577105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036677985","display_name":"Linda Gordon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181231927","display_name":"National University of Ireland","ror":"https://ror.org/00shsf120","country_code":"IE","type":"education","lineage":["https://openalex.org/I181231927"]},{"id":"https://openalex.org/I27577105","display_name":"University College Cork","ror":"https://ror.org/03265fv13","country_code":"IE","type":"education","lineage":["https://openalex.org/I27577105"]}],"countries":["IE"],"is_corresponding":false,"raw_author_name":"L.M. Gordon","raw_affiliation_strings":["Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland","institution_ids":["https://openalex.org/I181231927","https://openalex.org/I27577105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001640952","display_name":"Mark O'Mahony","orcid":"https://orcid.org/0000-0001-6224-640X","observed_orcids":["https://orcid.org/0000-0001-6224-640X"]},"institutions":[{"id":"https://openalex.org/I181231927","display_name":"National University of Ireland","ror":"https://ror.org/00shsf120","country_code":"IE","type":"education","lineage":["https://openalex.org/I181231927"]},{"id":"https://openalex.org/I27577105","display_name":"University College Cork","ror":"https://ror.org/03265fv13","country_code":"IE","type":"education","lineage":["https://openalex.org/I27577105"]}],"countries":["IE"],"is_corresponding":false,"raw_author_name":"M.M. O'Mahony","raw_affiliation_strings":["Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland","institution_ids":["https://openalex.org/I181231927","https://openalex.org/I27577105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114182676","display_name":"Niall D. O\u2019Leary","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181231927","display_name":"National University of Ireland","ror":"https://ror.org/00shsf120","country_code":"IE","type":"education","lineage":["https://openalex.org/I181231927"]},{"id":"https://openalex.org/I27577105","display_name":"University College Cork","ror":"https://ror.org/03265fv13","country_code":"IE","type":"education","lineage":["https://openalex.org/I27577105"]}],"countries":["IE"],"is_corresponding":false,"raw_author_name":"N.D. O'Leary","raw_affiliation_strings":["Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland","institution_ids":["https://openalex.org/I181231927","https://openalex.org/I27577105"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003887698","display_name":"Alan D. W. Dobson","orcid":"https://orcid.org/0000-0002-7713-5574","observed_orcids":["https://orcid.org/0000-0002-7713-5574"]},"institutions":[{"id":"https://openalex.org/I181231927","display_name":"National University of Ireland","ror":"https://ror.org/00shsf120","country_code":"IE","type":"education","lineage":["https://openalex.org/I181231927"]},{"id":"https://openalex.org/I27577105","display_name":"University College Cork","ror":"https://ror.org/03265fv13","country_code":"IE","type":"education","lineage":["https://openalex.org/I27577105"]}],"countries":["IE"],"is_corresponding":true,"raw_author_name":"A.D.W. Dobson","raw_affiliation_strings":["Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland","institution_ids":["https://openalex.org/I181231927","https://openalex.org/I27577105"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5003887698"],"corresponding_institution_ids":["https://openalex.org/I181231927","https://openalex.org/I27577105"],"apc_list":{"value":2500,"currency":"GBP","value_usd":3297},"apc_paid":null,"fwci":1.9503,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.82248864,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"239","issue":"2","first_page":"285","last_page":"293"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11136","display_name":"Enzyme-mediated dye degradation","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13736","display_name":"Steroid Chemistry and Biochemistry","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/indigo-biosynthesis","display_name":"indigo biosynthesis","score":0.8230000138282776},{"id":"https://openalex.org/keywords/gene-disruption","display_name":"gene disruption","score":0.8309999704360962},{"id":"https://openalex.org/keywords/acyl-coa-dehydrogenase","display_name":"acyl-CoA dehydrogenase","score":0.843999981880188},{"id":"https://openalex.org/keywords/novosphingobium-aromaticivorans","display_name":"Novosphingobium aromaticivorans","score":0.8709999918937683},{"id":"https://openalex.org/keywords/aromatic-hydrocarbon-degradation","display_name":"aromatic hydrocarbon degradation","score":0.6850000023841858},{"id":"https://openalex.org/keywords/gene-expression-regulation","display_name":"gene expression regulation","score":0.6880000233650208}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6181444525718689},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5691558718681335},{"id":"https://openalex.org/C2776834422","wikidata":"https://www.wikidata.org/wiki/Q5279660","display_name":"Dioxygenase","level":3,"score":0.5163310766220093},{"id":"https://openalex.org/C2780112459","wikidata":"https://www.wikidata.org/wiki/Q423462","display_name":"Fluoranthene","level":3,"score":0.5099376440048218},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.46208474040031433},{"id":"https://openalex.org/C203075996","wikidata":"https://www.wikidata.org/wiki/Q139677","display_name":"Operon","level":4,"score":0.44771236181259155},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.2885912358760834},{"id":"https://openalex.org/C2776891815","wikidata":"https://www.wikidata.org/wiki/Q422037","display_name":"Phenanthrene","level":2,"score":0.0},{"id":"https://openalex.org/C87355193","wikidata":"https://www.wikidata.org/wiki/Q411","display_name":"Astrobiology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005449","descriptor_name":"Fluorenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D007203","descriptor_name":"Indigo Carmine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007211","descriptor_name":"Indoles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015964","descriptor_name":"Gene Expression Regulation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044202","descriptor_name":"Pseudomonas alcaligenes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D044202","descriptor_name":"Pseudomonas alcaligenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049308","descriptor_name":"Dioxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.femsle.2004.08.046","doi":"https://doi.org/10.1016/j.femsle.2004.08.046","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.femsle.2004.08.046","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},{"id":"pmid:15476978","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15476978","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS microbiology letters","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1480869892","https://openalex.org/W1507281775","https://openalex.org/W1573484779","https://openalex.org/W1751341019","https://openalex.org/W1780083071","https://openalex.org/W1780544588","https://openalex.org/W1865262034","https://openalex.org/W1878804204","https://openalex.org/W1930963475","https://openalex.org/W1966939113","https://openalex.org/W1997562369","https://openalex.org/W2004003750","https://openalex.org/W2011261850","https://openalex.org/W2017129943","https://openalex.org/W2017583902","https://openalex.org/W2024347427","https://openalex.org/W2064814720","https://openalex.org/W2066969905","https://openalex.org/W2073755842","https://openalex.org/W2094123166","https://openalex.org/W2099772297","https://openalex.org/W2130911289","https://openalex.org/W2137475948","https://openalex.org/W2139669165","https://openalex.org/W2143296802","https://openalex.org/W2144634347","https://openalex.org/W2146440369","https://openalex.org/W2146884288","https://openalex.org/W2148327669","https://openalex.org/W2149078888","https://openalex.org/W2149353640","https://openalex.org/W2150297520","https://openalex.org/W2158714788","https://openalex.org/W2573542368"],"related_works":["https://openalex.org/W1780544588","https://openalex.org/W4294251913","https://openalex.org/W2026982131","https://openalex.org/W1541759828","https://openalex.org/W4385230796","https://openalex.org/W2893815130","https://openalex.org/W2012591853","https://openalex.org/W2528629896","https://openalex.org/W2339024793","https://openalex.org/W2944341375"],"abstract_inverted_index":{"A":[0],"novel":[1],"indole":[2,19],"dioxygenase":[3],"(idoA)":[4],"gene":[5,26,52,70,85,98],"has":[6,86],"been":[7,87],"cloned":[8,32],"from":[9,39,61],"Pseudomonas":[10],"alcaligenes":[11],"PA-10,":[12,105],"based":[13],"on":[14],"its":[15,107],"ability":[16,91],"to":[17,20,30,36,57,92,100,112,117,120],"convert":[18],"indigo.":[21],"The":[22,64,96],"chromosomally":[23],"encoded":[24,66],"idoA":[25,51,69,84,97],"exhibits":[27],"no":[28],"similarity":[29],"previously":[31],"naphthalene":[33],"dioxygenases":[34],"or":[35],"aromatic":[37],"oxygenases":[38],"other":[40],"species":[41],"at":[42],"the":[43,50,68,74,83,90],"nucleotide":[44],"level.":[45],"Phylogenetic":[46],"analysis":[47],"indicates":[48],"that":[49],"product":[53],"is":[54,71,109],"most":[55],"similar":[56],"an":[58],"acyl-CoA":[59],"dehydrogenase":[60],"Novosphingobium":[62],"aromaticivorans.":[63],"enzyme":[65],"by":[67],"essential":[72],"for":[73],"metabolism":[75],"of":[76],"fluoranthene,":[77],"since":[78],"a":[79],"mutant":[80],"in":[81,104],"which":[82],"disrupted":[88],"looses":[89],"degrade":[93],"this":[94],"compound.":[95],"appears":[99],"be":[101],"constitutively":[102],"expressed":[103],"but":[106],"expression":[108],"also":[110],"subject":[111],"regulation":[113],"following":[114],"prior":[115],"exposure":[116],"salicylate":[118],"and":[119],"fluoranthene":[121],"degradative":[122],"intermediates.":[123]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}
