{"id":"https://openalex.org/W2047054792","doi":"https://doi.org/10.1093/nar/5.4.1325","title":"Ability of modified forms of phenylalanine tRNA to stimulate guanosine pentaphosphate synthesis by the stringent factor-ribosome complex of E. coli","display_name":"Ability of modified forms of phenylalanine tRNA to stimulate guanosine pentaphosphate synthesis by the stringent factor-ribosome complex of E. coli","publication_year":1978,"publication_date":"1978-01-01","ids":{"openalex":"https://openalex.org/W2047054792","doi":"https://doi.org/10.1093/nar/5.4.1325","mag":"2047054792","pmid":"https://pubmed.ncbi.nlm.nih.gov/349503","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/342080"},"language":"en","primary_location":{"id":"doi:10.1093/nar/5.4.1325","doi":"https://doi.org/10.1093/nar/5.4.1325","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/5.4.1325","pdf_url":"https://academic.oup.com/nar/article-pdf/5/4/1325/3870238/5-4-1325.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/nar/article-pdf/5/4/1325/3870238/5-4-1325.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029509149","display_name":"James Ofengand","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"James Ofengand","raw_affiliation_strings":["Roche Institute of Molecular Biology Nutley, NJ 07110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Roche Institute of Molecular Biology Nutley, NJ 07110, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5080738139","display_name":"Richard Liou","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Richard Liou","raw_affiliation_strings":["Roche Institute of Molecular Biology Nutley, NJ 07110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Roche Institute of Molecular Biology Nutley, NJ 07110, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":0.7523,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":{"value":0.66422132,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"5","issue":"4","first_page":"1325","last_page":"1334"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9560999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9560999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.030700000002980232,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phenylalanine-trna","display_name":"phenylalanine tRNA","score":0.9570000171661377},{"id":"https://openalex.org/keywords/ribosome","display_name":"ribosome","score":0.6050000190734863},{"id":"https://openalex.org/keywords/p-ppgpp","display_name":"(p)ppGpp","score":0.8659999966621399},{"id":"https://openalex.org/keywords/4-thiouridine","display_name":"4-thiouridine","score":0.8259999752044678},{"id":"https://openalex.org/keywords/trna-modification","display_name":"tRNA modification","score":0.6600000262260437},{"id":"https://openalex.org/keywords/photoaffinity-labeling","display_name":"photoaffinity labeling","score":0.8270000219345093},{"id":"https://openalex.org/keywords/fluorescent-probes","display_name":"fluorescent probes","score":0.5389999747276306},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9789999723434448}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.897130012512207},{"id":"https://openalex.org/C2777995097","wikidata":"https://www.wikidata.org/wiki/Q422462","display_name":"Guanosine","level":2,"score":0.8005995750427246},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.7458022832870483},{"id":"https://openalex.org/C2777431362","wikidata":"https://www.wikidata.org/wiki/Q170545","display_name":"Phenylalanine","level":3,"score":0.6606281399726868},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.6423325538635254},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5445144772529602},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.49902939796447754},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.4875156581401825},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4760301411151886},{"id":"https://openalex.org/C2778301229","wikidata":"https://www.wikidata.org/wiki/Q169313","display_name":"Guanine","level":4,"score":0.44055694341659546},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3703387975692749},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.27604570984840393},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.2606275677680969},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14767852425575256},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.14520591497421265},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.1322181522846222}],"mesh":[{"descriptor_ui":"D000643","descriptor_name":"Ammonium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000643","descriptor_name":"Ammonium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000643","descriptor_name":"Ammonium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006150","descriptor_name":"Guanine Nucleotides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006150","descriptor_name":"Guanine Nucleotides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006150","descriptor_name":"Guanine Nucleotides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006159","descriptor_name":"Guanosine Tetraphosphate","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006159","descriptor_name":"Guanosine Tetraphosphate","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006159","descriptor_name":"Guanosine Tetraphosphate","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012269","descriptor_name":"Ribosomal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012269","descriptor_name":"Ribosomal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012269","descriptor_name":"Ribosomal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013891","descriptor_name":"Thiouridine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013891","descriptor_name":"Thiouridine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013891","descriptor_name":"Thiouridine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014529","descriptor_name":"Uridine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D014529","descriptor_name":"Uridine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D014529","descriptor_name":"Uridine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/nar/5.4.1325","doi":"https://doi.org/10.1093/nar/5.4.1325","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/5.4.1325","pdf_url":"https://academic.oup.com/nar/article-pdf/5/4/1325/3870238/5-4-1325.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:342080","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/342080","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:349503","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/349503","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1093/nar/5.4.1325","doi":"https://doi.org/10.1093/nar/5.4.1325","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/5.4.1325","pdf_url":"https://academic.oup.com/nar/article-pdf/5/4/1325/3870238/5-4-1325.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6","score":0.5400000214576721}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2047054792.pdf","grobid_xml":"https://content.openalex.org/works/W2047054792.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W255940250","https://openalex.org/W1172343711","https://openalex.org/W1526088607","https://openalex.org/W1534923971","https://openalex.org/W1555966296","https://openalex.org/W1677762627","https://openalex.org/W1900318200","https://openalex.org/W1969964298","https://openalex.org/W2004357841","https://openalex.org/W2004829645","https://openalex.org/W2027918802","https://openalex.org/W2042621443","https://openalex.org/W2049111069","https://openalex.org/W2050494626","https://openalex.org/W2052915258","https://openalex.org/W2058443519","https://openalex.org/W2060704465","https://openalex.org/W2134055885","https://openalex.org/W2407701564","https://openalex.org/W6601795639","https://openalex.org/W6608022050"],"related_works":["https://openalex.org/W2032417720","https://openalex.org/W2026092280","https://openalex.org/W2412197671","https://openalex.org/W2148579902","https://openalex.org/W2088944537","https://openalex.org/W2412260744","https://openalex.org/W2054812744","https://openalex.org/W2060644630","https://openalex.org/W2438069074","https://openalex.org/W2033032486"],"abstract_inverted_index":{"tRNA(Phe)":[0],"of":[1,39,54,66,83,96,104,131],"E.":[2],"coli,":[3],"modified":[4],"at":[5,77,87],"its":[6,16],"4-thiouridine":[7],"((4)Srd)":[8],"and":[9,34,74],"3-(3-amino-3-carboxypropyl)uridine":[10],"(nbt(3)Urd)":[11],"residues,":[12],"was":[13,25,42,47],"tested":[14],"for":[15,69,115,128],"ability":[17],"to":[18,32,111,138],"induce":[19],"(p)ppGpp":[20,84],"synthesis.":[21],"The":[22,44],"(4)Srd":[23],"residue":[24,46],"derivatized":[26,48],"with":[27,49,136],"the":[28,35,40,50,64,67,70,80,97,105,118,129],"p-azido-phenacyl":[29],"group,":[30],"cross-linked":[31],"Cyd(13),":[33],"borohydride":[36],"reduction":[37],"product":[38],"cross-link":[41],"prepared.":[43],"nbt(3)Urd":[45],"N-(4-azido-2-nitrophenyl)glycyl":[51],"group.":[52],"None":[53],"these":[55],"derivatives":[56],"had":[57],"more":[58],"than":[59],"a":[60],"minor":[61],"effect":[62,76],"on":[63,79,101],"affinity":[65],"tRNA":[68,98,106],"stringent":[71,119],"factor-ribosome":[72,120],"complex,":[73],"no":[75],"all":[78],"maximum":[81],"velocity":[82],"synthesis,":[85],"either":[86],"2":[88],"or":[89,133],"82":[90],"mM":[91],"NH(4)Cl.":[92],"These":[93],"two":[94],"regions":[95],"which":[99,137],"are":[100],"opposite":[102],"faces":[103],"molecule":[107],"do":[108],"not":[109],"appear":[110],"be":[112],"structurally":[113],"important":[114],"recognition":[116],"by":[117],"complex.":[121],"They":[122],"may":[123],"provide":[124],"useful":[125],"sites,":[126],"therefore,":[127],"introduction":[130],"photoaffinity":[132],"fluorescent":[134],"probes":[135],"study":[139],"tRNA-stringent":[140],"factor":[141],"recognition.":[142]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}