{"id":"https://openalex.org/W2046515690","doi":"https://doi.org/10.1021/pr0503073","title":"Phosphoproteomic Analysis of Rat Liver by High Capacity IMAC and LC\u2212MS/MS","display_name":"Phosphoproteomic Analysis of Rat Liver by High Capacity IMAC and LC\u2212MS/MS","publication_year":2005,"publication_date":"2005-12-01","ids":{"openalex":"https://openalex.org/W2046515690","doi":"https://doi.org/10.1021/pr0503073","mag":"2046515690","pmid":"https://pubmed.ncbi.nlm.nih.gov/16396499"},"language":"en","primary_location":{"id":"doi:10.1021/pr0503073","doi":"https://doi.org/10.1021/pr0503073","is_oa":false,"landing_page_url":"https://doi.org/10.1021/pr0503073","pdf_url":null,"source":{"id":"https://openalex.org/S74367812","display_name":"Journal of Proteome Research","issn_l":"1535-3893","issn":["1535-3893","1535-3907"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Proteome Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Katrin Moser","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katrin Moser","raw_affiliation_strings":["Biological Engineering Division, MIT, Cambridge, Massachusetts 02139","Biological Engineering Division, MIT, Cambridge, Massachusetts 02139, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Engineering Division, MIT, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Biological Engineering Division, MIT, Cambridge, Massachusetts 02139, USA","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002079869","display_name":"Forest M. White","orcid":"https://orcid.org/0000-0002-1545-1651","observed_orcids":["https://orcid.org/0000-0002-1545-1651"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Forest M. White","raw_affiliation_strings":["Biological Engineering Division, MIT, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Engineering Division, MIT, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5002079869"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":8.0939,"has_fulltext":false,"cited_by_count":148,"citation_normalized_percentile":{"value":0.98665806,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"5","issue":"1","first_page":"98","last_page":"104"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11465","display_name":"Drug-Induced Hepatotoxicity and Protection","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-phosphorylation","display_name":"protein phosphorylation","score":0.871999979019165},{"id":"https://openalex.org/keywords/immobilized-metal-affinity-chromatography","display_name":"immobilized metal affinity chromatography","score":0.8659999966621399},{"id":"https://openalex.org/keywords/phosphorylation-site-identification","display_name":"phosphorylation site identification","score":0.7459999918937683},{"id":"https://openalex.org/keywords/hepatotoxicity","display_name":"hepatotoxicity","score":0.8040000200271606},{"id":"https://openalex.org/keywords/small-molecule-drugs","display_name":"small molecule drugs","score":0.7990000247955322},{"id":"https://openalex.org/keywords/signal-transduction","display_name":"signal transduction","score":0.6520000100135803},{"id":"https://openalex.org/keywords/rat-liver","display_name":"rat liver","score":0.8489999771118164},{"id":"https://openalex.org/keywords/drug-toxicity-assessment","display_name":"drug toxicity assessment","score":0.3659999966621399}],"concepts":[{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.7779677510261536},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.6358892917633057},{"id":"https://openalex.org/C87325107","wikidata":"https://www.wikidata.org/wiki/Q7251493","display_name":"Protein phosphorylation","level":4,"score":0.6011294722557068},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.5457621216773987},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.522657573223114},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4992375373840332},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.4391372799873352},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.43542978167533875},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4155155122280121},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36844921112060547},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2601245045661926},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.24167689681053162},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010748","descriptor_name":"Phosphopeptides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D010750","descriptor_name":"Phosphoproteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D010750","descriptor_name":"Phosphoproteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010770","descriptor_name":"Phosphotransferases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011916","descriptor_name":"Rats, Inbred F344","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/pr0503073","doi":"https://doi.org/10.1021/pr0503073","is_oa":false,"landing_page_url":"https://doi.org/10.1021/pr0503073","pdf_url":null,"source":{"id":"https://openalex.org/S74367812","display_name":"Journal of Proteome Research","issn_l":"1535-3893","issn":["1535-3893","1535-3907"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Proteome Research","raw_type":"journal-article"},{"id":"pmid:16396499","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16396499","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of proteome research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3909382221","display_name":"MIT Center for Environmental Health Sciences","funder_award_id":"P30ES002109","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G482854693","display_name":null,"funder_award_id":"DK42816","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1966606518","https://openalex.org/W1979456040","https://openalex.org/W2004704197","https://openalex.org/W2011147958","https://openalex.org/W2025036829","https://openalex.org/W2030157681","https://openalex.org/W2038986538","https://openalex.org/W2042412160","https://openalex.org/W2046976278","https://openalex.org/W2054038703","https://openalex.org/W2085037886","https://openalex.org/W2100061987","https://openalex.org/W2110663167","https://openalex.org/W2113569709","https://openalex.org/W2129777487","https://openalex.org/W2155880596","https://openalex.org/W2404695032"],"related_works":["https://openalex.org/W1985475405","https://openalex.org/W2467704906","https://openalex.org/W2140961919","https://openalex.org/W2463495909","https://openalex.org/W2313673075","https://openalex.org/W2280759106","https://openalex.org/W2354394277","https://openalex.org/W1800605021","https://openalex.org/W2062234646","https://openalex.org/W3196786708"],"abstract_inverted_index":{"Proper":[0],"liver":[1,25,46,126,159,251],"function":[2,26],"is":[3,99],"crucial":[4],"for":[5,213],"metabolism":[6,181],"control":[7],"and":[8,27,32,44,88,165,185,254],"to":[9,41,68,79,113,216,233,246],"clear":[10],"toxic":[11,69],"substances":[12],"from":[13,117,124,143],"the":[14,21,85,135,158,172,199,248],"bloodstream.":[15],"Many":[16],"small-molecule":[17],"therapeutics":[18],"accumulate":[19],"in":[20,30,66,84,95,134,147,171,180,239],"liver,":[22,86,149],"negatively":[23],"impacting":[24],"often":[28],"resulting":[29],"hepatotoxicity":[31,43],"cell":[33],"death.":[34],"Several":[35],"analytical":[36],"methods":[37,53],"are":[38],"currently":[39],"utilized":[40,104],"evaluate":[42,255],"monitor":[45],"function.":[47],"To":[48,75],"date,":[49],"none":[50],"of":[51,71,90,121,130,137,157,198,203,250,258],"these":[52,223],"have":[54,103],"specifically":[55],"targeted":[56],"protein":[57,92,190],"phosphorylation-mediated":[58],"signal":[59],"transduction":[60],"pathways":[61,83],"which":[62],"should":[63],"be":[64,217,231],"altered":[65],"response":[67],"effects":[70,249],"small":[72,204],"molecule":[73],"therapeutics.":[74],"develop":[76],"novel":[77,167],"assays":[78,215],"probe":[80],"specific":[81,91],"signaling":[82,187],"identification":[87,136],"quantification":[89],"phosphorylation":[93,141,163,191,236],"sites":[94,142,164,168,176,237],"this":[96,220,228],"complex":[97],"organ":[98],"necessary.":[100],"Here,":[101],"we":[102],"an":[105],"optimized":[106],"immobilized":[107],"metal":[108],"affinity":[109],"chromatography":[110],"(IMAC)":[111],"protocol":[112],"enrich":[114],"phosphorylated":[115],"peptides":[116,132],"a":[118,150,242],"tryptic":[119],"digest":[120],"proteins":[122,146,178],"isolated":[123],"whole":[125],"lysate.":[127],"LC-MS/MS":[128],"analysis":[129,192,202,209],"IMAC-enriched":[131],"resulted":[133],"more":[138],"than":[139],"300":[140],"over":[144,153],"200":[145],"rat":[148],"significant":[151],"advance":[152],"previously":[154],"published":[155],"analyses":[156],"phosphoproteome.":[160],"Previously":[161],"characterized":[162],"potentially":[166,211],"were":[169],"identified":[170],"current":[173],"study,":[174],"including":[175],"on":[177],"implicated":[179],"regulation,":[182],"transcription,":[183],"translation,":[184],"canonical":[186],"pathways.":[188],"Moreover,":[189],"was":[193],"performed":[194,218],"without":[195],"prior":[196],"fractionation":[197],"sample,":[200],"enabling":[201],"sample":[205],"amounts":[206],"while":[207],"minimizing":[208],"time,":[210],"allowing":[212],"high-throughput":[214],"with":[219,241],"methodology.":[221],"From":[222],"data,":[224],"it":[225],"appears":[226],"that":[227],"methodology":[229],"can":[230],"used":[232],"identify":[234],"new":[235,259],"and,":[238],"combination":[240],"stable":[243],"isotope-labeling":[244],"step,":[245],"investigate":[247],"diseases,":[252],"cancer":[253],"potential":[256],"toxicology":[257],"drug":[260],"substances.":[261]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}