{"id":"https://openalex.org/W2045549239","doi":"https://doi.org/10.1109/iembs.2011.6090167","title":"Electroporation based gene therapy \u2014 From the bench to the bedside","display_name":"Electroporation based gene therapy \u2014 From the bench to the bedside","publication_year":2011,"publication_date":"2011-08-01","ids":{"openalex":"https://openalex.org/W2045549239","doi":"https://doi.org/10.1109/iembs.2011.6090167","mag":"2045549239","pmid":"https://pubmed.ncbi.nlm.nih.gov/22254415"},"language":"en","primary_location":{"id":"doi:10.1109/iembs.2011.6090167","doi":"https://doi.org/10.1109/iembs.2011.6090167","is_oa":false,"landing_page_url":"https://doi.org/10.1109/iembs.2011.6090167","pdf_url":null,"source":{"id":"https://openalex.org/S4363608786","display_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","issn_l":"2375-7477","issn":["2375-7477"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["doyens","jufo-1","ki-jl-1","medline","russia-white-list-3"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057362562","display_name":"Richard F. Heller","orcid":"https://orcid.org/0000-0003-1899-3859","observed_orcids":["https://orcid.org/0000-0003-1899-3859"]},"institutions":[{"id":"https://openalex.org/I81365321","display_name":"Old Dominion University","ror":"https://ror.org/04zjtrb98","country_code":"US","type":"education","lineage":["https://openalex.org/I81365321"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard Heller","raw_affiliation_strings":["Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA. rheller@odu.edu","Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA. rheller@odu.edu","institution_ids":["https://openalex.org/I81365321"]},{"raw_affiliation_string":"Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA","institution_ids":["https://openalex.org/I81365321"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110086104","display_name":"Shawna A. Shirley","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I81365321","display_name":"Old Dominion University","ror":"https://ror.org/04zjtrb98","country_code":"US","type":"education","lineage":["https://openalex.org/I81365321"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shawna Shirley","raw_affiliation_strings":["Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","institution_ids":["https://openalex.org/I81365321"]},{"raw_affiliation_string":"Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA","institution_ids":["https://openalex.org/I81365321"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024979833","display_name":"Siqi Guo","orcid":"https://orcid.org/0000-0002-7280-7888","observed_orcids":["https://orcid.org/0000-0002-7280-7888"]},"institutions":[{"id":"https://openalex.org/I81365321","display_name":"Old Dominion University","ror":"https://ror.org/04zjtrb98","country_code":"US","type":"education","lineage":["https://openalex.org/I81365321"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Siqi Guo","raw_affiliation_strings":["Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","institution_ids":["https://openalex.org/I81365321"]},{"raw_affiliation_string":"Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA","institution_ids":["https://openalex.org/I81365321"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017826169","display_name":"Amy Donate","orcid":"https://orcid.org/0000-0002-0481-5675","observed_orcids":["https://orcid.org/0000-0002-0481-5675"]},"institutions":[{"id":"https://openalex.org/I81365321","display_name":"Old Dominion University","ror":"https://ror.org/04zjtrb98","country_code":"US","type":"education","lineage":["https://openalex.org/I81365321"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amy Donate","raw_affiliation_strings":["Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","institution_ids":["https://openalex.org/I81365321"]},{"raw_affiliation_string":"Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA","institution_ids":["https://openalex.org/I81365321"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048547302","display_name":"Lore\u00e9 C. Heller","orcid":"https://orcid.org/0000-0001-8084-0787","observed_orcids":["https://orcid.org/0000-0001-8084-0787"]},"institutions":[{"id":"https://openalex.org/I81365321","display_name":"Old Dominion University","ror":"https://ror.org/04zjtrb98","country_code":"US","type":"education","lineage":["https://openalex.org/I81365321"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Loree Heller","raw_affiliation_strings":["Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frank Reidy Research Center of Bioelectrics, Old Dominion University, Norfolk, VA, USA","institution_ids":["https://openalex.org/I81365321"]},{"raw_affiliation_string":"Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA","institution_ids":["https://openalex.org/I81365321"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I81365321"],"apc_list":null,"apc_paid":null,"fwci":20.4419,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":1.0,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"2011","issue":null,"first_page":"736","last_page":"738"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10704","display_name":"Advancements in Transdermal Drug Delivery","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/3003","display_name":"Pharmaceutical Science"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/electroporation","display_name":"electroporation","score":0.9919999837875366},{"id":"https://openalex.org/keywords/plasmid-dna-delivery","display_name":"plasmid DNA delivery","score":0.9229999780654907},{"id":"https://openalex.org/keywords/dna-vaccine","display_name":"DNA vaccine","score":0.6790000200271606},{"id":"https://openalex.org/keywords/wound-healing","display_name":"wound healing","score":0.847000002861023},{"id":"https://openalex.org/keywords/angiogenesis","display_name":"angiogenesis","score":0.9919999837875366},{"id":"https://openalex.org/keywords/vascular-endothelial-growth-factor","display_name":"vascular endothelial growth factor","score":0.9879999756813049},{"id":"https://openalex.org/keywords/hepatitis-b-surface-antigen","display_name":"hepatitis B surface antigen","score":0.9769999980926514},{"id":"https://openalex.org/keywords/cytokine-gene-therapy","display_name":"cytokine gene therapy","score":0.753000020980835},{"id":"https://openalex.org/keywords/cutaneous-melanoma","display_name":"cutaneous melanoma","score":0.8949999809265137},{"id":"https://openalex.org/keywords/il-12","display_name":"IL-12","score":0.8830000162124634},{"id":"https://openalex.org/keywords/tumor-necrosis","display_name":"tumor necrosis","score":0.5090000033378601},{"id":"https://openalex.org/keywords/lymphocytic-infiltration","display_name":"lymphocyte infiltration","score":0.7960000038146973}],"concepts":[{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.9484644532203674},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.8060097694396973},{"id":"https://openalex.org/C135983454","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Gene delivery","level":4,"score":0.7053639888763428},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6439396142959595},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5208771228790283},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5151365995407104},{"id":"https://openalex.org/C2780394083","wikidata":"https://www.wikidata.org/wiki/Q539568","display_name":"Angiogenesis","level":2,"score":0.4834481179714203},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.47566503286361694},{"id":"https://openalex.org/C168112101","wikidata":"https://www.wikidata.org/wiki/Q1502320","display_name":"Gene gun","level":5,"score":0.431270956993103},{"id":"https://openalex.org/C2780269544","wikidata":"https://www.wikidata.org/wiki/Q1509074","display_name":"Wound healing","level":2,"score":0.4217597246170044},{"id":"https://openalex.org/C167508917","wikidata":"https://www.wikidata.org/wiki/Q578537","display_name":"DNA vaccination","level":4,"score":0.36383140087127686},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3576718270778656},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3390903174877167},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2595462203025818},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.23361364006996155},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000639","qualifier_name":"trends","is_major_topic":true},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":"Q000639","qualifier_name":"trends","is_major_topic":true},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":"Q000639","qualifier_name":"trends","is_major_topic":true},{"descriptor_ui":"D057170","descriptor_name":"Translational Research, Biomedical","qualifier_ui":"Q000639","qualifier_name":"trends","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1109/iembs.2011.6090167","doi":"https://doi.org/10.1109/iembs.2011.6090167","is_oa":false,"landing_page_url":"https://doi.org/10.1109/iembs.2011.6090167","pdf_url":null,"source":{"id":"https://openalex.org/S4363608786","display_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","issn_l":"2375-7477","issn":["2375-7477"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["doyens","jufo-1","ki-jl-1","medline","russia-white-list-3"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society","raw_type":"proceedings-article"},{"id":"pmid:22254415","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22254415","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9763000011444092,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1711365341","display_name":null,"funder_award_id":"CA122518","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G4993976806","display_name":null,"funder_award_id":"EB005441","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"},{"id":"https://openalex.org/G5922881526","display_name":"Therapeutic Potential of IL-15 Plasmid Delivery to Tumors Using Electroporation","funder_award_id":"R01CA122518","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6194890768","display_name":"Electroporation System for Cutaneous Gene Transfer","funder_award_id":"R01EB005441","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1622592612","https://openalex.org/W1966463645","https://openalex.org/W1970510706","https://openalex.org/W1974571310","https://openalex.org/W2016101110","https://openalex.org/W2016724596","https://openalex.org/W2039942347","https://openalex.org/W2053525254","https://openalex.org/W2054457322","https://openalex.org/W2057822452","https://openalex.org/W2063802293","https://openalex.org/W2066606615","https://openalex.org/W2067738057","https://openalex.org/W2075010947","https://openalex.org/W2076337048","https://openalex.org/W2077850392","https://openalex.org/W2122387392","https://openalex.org/W2133053113","https://openalex.org/W2136535174","https://openalex.org/W2326599775","https://openalex.org/W2400389955","https://openalex.org/W3024831056"],"related_works":["https://openalex.org/W144445175","https://openalex.org/W1972913617","https://openalex.org/W2104979755","https://openalex.org/W2095200130","https://openalex.org/W2400249100","https://openalex.org/W1853640651","https://openalex.org/W2054020019","https://openalex.org/W2270410932","https://openalex.org/W1566545437","https://openalex.org/W2010105484"],"abstract_inverted_index":{"A":[0],"critical":[1],"aspect":[2],"of":[3,9,20,93,121,148,161,175,181,205,250],"gene":[4,251],"transfer":[5,44,252],"is":[6,37,50,138,237],"effective":[7,32],"delivery":[8,18,92,124,241],"the":[10,13,46,69,119,142,146,154,185,248,255,258],"transgene":[11],"to":[12,30,41,45,68,123,229,246,257],"appropriate":[14],"target.":[15],"Electrically":[16],"mediated":[17],"(electroporation)":[19],"plasmid":[21,39,74,95,125,137,231],"DNA":[22,40,66,126,232],"has":[23,81,116,243],"been":[24,234],"accepted":[25],"as":[26,130,187,189],"a":[27,73,94,131,157,213],"viable":[28],"approach":[29,242],"achieve":[31],"delivery.":[33],"One":[34],"promising":[35],"area":[36],"delivering":[38,65],"skin.":[42,70],"Gene":[43],"skin":[47],"with":[48,169],"electroporation":[49,122,228],"currently":[51],"being":[52],"evaluated":[53],"for":[54,57,60,64,76,134,156],"its":[55],"potential":[56,132,245],"inducing":[58],"angiogenesis":[59],"wound":[61,84],"healing":[62,85],"and":[63],"vaccines":[67],"Experiments":[71],"utilizing":[72,227],"encoding":[75,96,127],"vascular":[77],"endothelial":[78],"growth":[79],"factor":[80],"demonstrated":[82,102],"how":[83],"could":[86],"be":[87,108],"accelerated.":[88],"In":[89],"another":[90],"study,":[91],"Hepatitis":[97],"B":[98],"surface":[99],"antigen":[100],"have":[101,233],"that":[103,239],"high":[104],"antibody":[105],"titers":[106],"can":[107],"induced":[109],"after":[110],"two":[111],"applications":[112],"(prime/boost).":[113],"Our":[114],"laboratory":[115],"also":[117],"examined":[118],"use":[120],"various":[128],"cytokines":[129],"therapy":[133,217],"melanoma.":[135],"The":[136],"injected":[139],"directly":[140],"into":[141],"tumor":[143,186],"followed":[144],"by":[145],"administration":[147],"electroporation.":[149],"Extensive":[150],"preclinical":[151],"work":[152],"provided":[153],"rationale":[155],"Phase":[158],"I":[159],"proof":[160],"concept":[162],"first":[163],"in":[164,167,196,200,207],"human":[165],"trial":[166,222],"patients":[168,199],"accessible":[170],"cutaneous":[171],"melanoma":[172,182],"metastases.":[173],"Biopsies":[174],"treated":[176],"lesions":[177,209],"showed":[178],"significant":[179],"necrosis":[180],"cells":[183],"within":[184],"well":[188],"IL-12":[190],"expression.":[191],"Lymphocytic":[192],"infiltrate":[193],"was":[194,212,218],"observed":[195],"biopsies":[197],"from":[198,254],"several":[201,223],"cohorts.":[202],"Clinical":[203],"evidence":[204],"responses":[206],"untreated":[208],"suggested":[210],"there":[211],"systemic":[214],"response":[215],"following":[216],"observed.":[219],"Since":[220],"this":[221,240],"other":[224],"clinical":[225],"studies":[226],"deliver":[230],"initiated.":[235],"It":[236],"clear":[238],"tremendous":[244],"facilitate":[247],"translation":[249],"protocols":[253],"bench":[256],"bedside.":[259]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}