{"id":"https://openalex.org/W2043799102","doi":"https://doi.org/10.1172/jci111706","title":"Biosynthesis of factor V in isolated guinea pig megakaryocytes.","display_name":"Biosynthesis of factor V in isolated guinea pig megakaryocytes.","publication_year":1985,"publication_date":"1985-02-01","ids":{"openalex":"https://openalex.org/W2043799102","doi":"https://doi.org/10.1172/jci111706","mag":"2043799102","pmid":"https://pubmed.ncbi.nlm.nih.gov/3973008","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/423488"},"language":"en","primary_location":{"id":"doi:10.1172/jci111706","doi":"https://doi.org/10.1172/jci111706","is_oa":true,"landing_page_url":"https://doi.org/10.1172/jci111706","pdf_url":"http://www.jci.org/articles/view/111706/files/pdf","source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://www.jci.org/articles/view/111706/files/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110157179","display_name":"Hui Chong Chiu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210122924","display_name":"Temple University Health System","ror":"https://ror.org/02fhvxj45","country_code":"US","type":"education","lineage":["https://openalex.org/I4210122924"]},{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H C Chiu","raw_affiliation_strings":["Thrombosis Research Center and Hematology-Oncology Section, Department of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thrombosis Research Center and Hematology-Oncology Section, Department of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I4210122924","https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111858661","display_name":"P K Schick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210122924","display_name":"Temple University Health System","ror":"https://ror.org/02fhvxj45","country_code":"US","type":"education","lineage":["https://openalex.org/I4210122924"]},{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P K Schick","raw_affiliation_strings":["Thrombosis Research Center and Hematology-Oncology Section, Department of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thrombosis Research Center and Hematology-Oncology Section, Department of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I4210122924","https://openalex.org/I84392919"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5104652394","display_name":"Robert W. Colman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210122924","display_name":"Temple University Health System","ror":"https://ror.org/02fhvxj45","country_code":"US","type":"education","lineage":["https://openalex.org/I4210122924"]},{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R W Colman","raw_affiliation_strings":["Thrombosis Research Center and Hematology-Oncology Section, Department of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thrombosis Research Center and Hematology-Oncology Section, Department of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I4210122924","https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5200,"currency":"USD","value_usd":5200},"apc_paid":null,"fwci":3.5411,"has_fulltext":true,"cited_by_count":70,"citation_normalized_percentile":{"value":0.92899005,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"75","issue":"2","first_page":"339","last_page":"346"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.6060000061988831,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.6060000061988831,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.37619999051094055,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.011300000362098217,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/factor-v","display_name":"factor V","score":0.7599999904632568},{"id":"https://openalex.org/keywords/megakaryocytes","display_name":"megakaryocytes","score":0.9620000123977661},{"id":"https://openalex.org/keywords/platelets","display_name":"platelets","score":0.6970000267028809},{"id":"https://openalex.org/keywords/alpha-granules","display_name":"alpha-granules","score":0.7960000038146973},{"id":"https://openalex.org/keywords/coagulation-cofactors","display_name":"coagulation cofactors","score":0.7870000004768372},{"id":"https://openalex.org/keywords/guinea-pig","display_name":"guinea pig","score":0.6830000281333923},{"id":"https://openalex.org/keywords/coagulant-activity","display_name":"coagulant activity","score":0.6150000095367432},{"id":"https://openalex.org/keywords/coagulation-inhibition","display_name":"coagulation inhibition","score":0.3799999952316284},{"id":"https://openalex.org/keywords/megakaryopoiesis","display_name":"megakaryopoiesis","score":0.6399999856948853}],"concepts":[{"id":"https://openalex.org/C2778815084","wikidata":"https://www.wikidata.org/wiki/Q79803","display_name":"Guinea pig","level":2,"score":0.7506637573242188},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.7113046050071716},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5911293625831604},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.5871933698654175},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5170591473579407},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5143715143203735},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.451386958360672},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4162830412387848},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.33864185214042664},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3310650587081909},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2500509023666382},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.13817763328552246}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001792","descriptor_name":"Blood Platelets","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005165","descriptor_name":"Factor V","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005165","descriptor_name":"Factor V","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D005165","descriptor_name":"Factor V","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D005165","descriptor_name":"Factor V","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D006168","descriptor_name":"Guinea Pigs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008533","descriptor_name":"Megakaryocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010949","descriptor_name":"Plasma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1172/jci111706","doi":"https://doi.org/10.1172/jci111706","is_oa":true,"landing_page_url":"https://doi.org/10.1172/jci111706","pdf_url":"http://www.jci.org/articles/view/111706/files/pdf","source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:423488","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/423488","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Invest","raw_type":"Text"},{"id":"pmid:3973008","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3973008","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of clinical investigation","raw_type":"Comparative Study"}],"best_oa_location":{"id":"doi:10.1172/jci111706","doi":"https://doi.org/10.1172/jci111706","is_oa":true,"landing_page_url":"https://doi.org/10.1172/jci111706","pdf_url":"http://www.jci.org/articles/view/111706/files/pdf","source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.46810001134872437,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G173662705","display_name":null,"funder_award_id":"HL 14217","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2043799102.pdf","grobid_xml":"https://content.openalex.org/works/W2043799102.grobid-xml"},"referenced_works_count":28,"referenced_works":["https://openalex.org/W38611617","https://openalex.org/W178468969","https://openalex.org/W1484585910","https://openalex.org/W1519085965","https://openalex.org/W1532528898","https://openalex.org/W1538941022","https://openalex.org/W1601861562","https://openalex.org/W1604706922","https://openalex.org/W1873581512","https://openalex.org/W1980662597","https://openalex.org/W1983195876","https://openalex.org/W1988451474","https://openalex.org/W1994419975","https://openalex.org/W2017423159","https://openalex.org/W2018289835","https://openalex.org/W2029845477","https://openalex.org/W2032611772","https://openalex.org/W2038680652","https://openalex.org/W2039205622","https://openalex.org/W2068346921","https://openalex.org/W2073352371","https://openalex.org/W2100837269","https://openalex.org/W2123863764","https://openalex.org/W2136185838","https://openalex.org/W2142480761","https://openalex.org/W2146307479","https://openalex.org/W2395205304","https://openalex.org/W2945433919"],"related_works":["https://openalex.org/W2888938237","https://openalex.org/W167595690","https://openalex.org/W2021504120","https://openalex.org/W2555361697","https://openalex.org/W191075882","https://openalex.org/W1994157173","https://openalex.org/W1965892255","https://openalex.org/W2223479787","https://openalex.org/W7024677969","https://openalex.org/W1874023063"],"abstract_inverted_index":{"Although":[0],"platelets":[1,183],"contain":[2,184,237],"Factor":[3,30,35,55,66,77,82,116,139,154,170,187,238,265,278,285,296,306,328,363,376,401,409],"V,":[4,364],"localized":[5],"primarily":[6],"in":[7,16,143,157,173,315,331],"the":[8,10,271,316,338,366,385,407],"alpha-granules,":[9],"origin":[11],"of":[12,153,169,220,274,318,337,345,352,371,375,400],"this":[13],"coagulation":[14],"cofactor":[15],"these":[17],"cells":[18],"is":[19,142,287,298],"not":[20,412],"known.":[21],"We":[22],"therefore":[23],"explored":[24],"whether":[25],"isolated":[26,404],"megakaryocytes":[27,122,233,243],"could":[28],"biosynthesize":[29],"V.":[31,56,67],"Guinea":[32],"pig":[33,111,121,159,198,302,359],"plasma":[34,112,160,175,225,327,362],"V":[36,78,83,117,140,155,171,188,239,258,266,279,286,297,307,377,402,410],"coagulant":[37,267],"activity":[38,268,273],"was":[39,123,161,308,320,326,403],"demonstrated":[40],"to":[41,63,74,80,236],"be":[42],"neutralized":[43,270],"by":[44,103,240,300,312],"human":[45,54,65,76,174,182,218,256,361],"monoclonal":[46],"and":[47,79,93,210,226,248,269,289,314,335,348,360,389,393],"rabbit":[48,276],"polyclonal":[49],"antibodies":[50,58,69],"directed":[51],"monospecifically":[52],"against":[53],"These":[57,68,292],"had":[59,70,368,411],"been":[60,71,413],"used":[61,72],"earlier":[62,73],"purify":[64,75],"quantify":[81],"antigen":[84,156,172,189],"concentration,":[85],"respectively":[86],"(1983.":[87],"Chiu,":[88],"H.":[89],"C.,":[90],"E.":[91],"Whitaker,":[92],"R.":[94],"W.":[95],"Colman.":[96],"J.":[97],"Clin.":[98],"Invest.":[99],"72:493-503).":[100],"As":[101],"determined":[102],"a":[104,114,144,255,275,342,349,398],"competitive":[105],"enzyme-linked":[106],"immunosorbent":[107],"assay":[108],"with":[109,217,246,357],"guinea":[110,120,158,197,301,358],"as":[113,137,141,355],"standard,":[115],"solubilized":[118],"from":[119],"present":[124],"at":[125],"0.098":[126],"+/-":[127,147,164,177,191,201,206,212,222,228],"0.018":[128],"micrograms/10(5)":[129],"cells.":[130],"Each":[131],"megakaryocyte":[132,284,305,408],"contained":[133],"about":[134],"500":[135],"times":[136],"much":[138],"platelet":[145],"(0.234":[146],"0.180":[148],"micrograms/10(8)":[149,193],"platelets).":[150],"The":[151,242,261],"content":[152],"greater":[162],"(27.0":[163],"3.0":[165],"micrograms/ml)":[166],"than":[167,195,325],"that":[168,283,295,397,406],"(11.1":[176],"0.4":[178],"micrograms/ml).":[179],"In":[180],"contrast,":[181],"ninefold":[185],"more":[186,309,321],"(2.01":[190],"1.09":[192],"platelets)":[194],"do":[196],"were":[199,234,244,253,378],"2.85":[200],"0.30":[202],"U/ml":[203,224],"plasma,":[204],"0.022":[205],"0.012":[207],"U/10(8)":[208,230],"platelets,":[209],"0.032":[211],"0.03":[213],"U/10(5)":[214],"megakaryocytes,":[215],"compared":[216,356],"values":[219],"0.98":[221],"0.02":[223],"0.124":[227],"0.064":[229],"platelets.":[231],"Isolated":[232],"found":[235],"cytoimmunofluorescence.":[241],"incubated":[245],"[35S]methionine,":[247],"radiolabeled":[249],"intracellular":[250],"proteins":[251],"purified":[252,262,339],"on":[254],"anti-Factor":[257],"immunoaffinity":[259],"column.":[260],"protein":[263,367],"exhibited":[264],"inhibitory":[272],"antihuman":[277],"antibody,":[280],"which":[281],"suggests":[282],"functionally":[288],"antigenically":[290],"intact.":[291],"results":[293],"indicate":[294],"synthesized":[299],"megakaryocytes.":[303],"Nonetheless,":[304],"slowly":[310],"activated":[311],"thrombin":[313,391],"absence":[317],"calcium":[319],"stable":[322],"after":[323],"activation":[324],"Va.":[329],"Electrophoresis":[330],"sodium":[332],"dodecyl":[333],"sulfate":[334],"autoradiography":[336],"molecule":[340],"showed":[341],"major":[343],"band":[344,351],"Mr":[346,353,370],"380,000":[347],"minor":[350],"350,000,":[354],"where":[365],"an":[369],"350,000.":[372],"Both":[373],"forms":[374],"substrates":[379],"for":[380,384],"thrombin.":[381],"Possible":[382],"explanations":[383],"higher":[386],"molecular":[387],"weight":[388],"different":[390],"sensitivity":[392],"stability":[394],"observed":[395],"are":[396],"precursor":[399],"or":[405],"fully":[414],"processed":[415],"before":[416],"isolation.":[417]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}