{"id":"https://openalex.org/W2043333387","doi":"https://doi.org/10.1016/0014-4827(86)90356-3","title":"Efficient immortalization and morphological transformation of human fibroblasts by transfection with SV40 DNA linked to a dominant marker","display_name":"Efficient immortalization and morphological transformation of human fibroblasts by transfection with SV40 DNA linked to a dominant marker","publication_year":1986,"publication_date":"1986-02-01","ids":{"openalex":"https://openalex.org/W2043333387","doi":"https://doi.org/10.1016/0014-4827(86)90356-3","mag":"2043333387","pmid":"https://pubmed.ncbi.nlm.nih.gov/3002824"},"language":"en","primary_location":{"id":"doi:10.1016/0014-4827(86)90356-3","doi":"https://doi.org/10.1016/0014-4827(86)90356-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0014-4827(86)90356-3","pdf_url":null,"source":{"id":"https://openalex.org/S132505712","display_name":"Experimental Cell Research","issn_l":"0014-4827","issn":["0014-4827","1090-2422"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental Cell Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111628871","display_name":"Lynne V. Mayne","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Lynne V. Mayne","raw_affiliation_strings":["Cell and Molecular Biology Laboratory, School of Biological Sciences, University of Sussex, Palmer, Brighton BN1 9QG UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell and Molecular Biology Laboratory, School of Biological Sciences, University of Sussex, Palmer, Brighton BN1 9QG UK","institution_ids":["https://openalex.org/I162608824"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111740359","display_name":"Anne Priestley","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Anne Priestley","raw_affiliation_strings":["Medical Research Council, Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9RP, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council, Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9RP, UK","institution_ids":["https://openalex.org/I162608824","https://openalex.org/I90344618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101855287","display_name":"Michael R. James","orcid":"https://orcid.org/0000-0002-4918-2998","observed_orcids":["https://orcid.org/0000-0002-4918-2998"]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Michael R. James","raw_affiliation_strings":["Medical Research Council, Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9RP, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council, Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9RP, UK","institution_ids":["https://openalex.org/I162608824","https://openalex.org/I90344618"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111420269","display_name":"Julian F. Burke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Julian F. Burke","raw_affiliation_strings":["Medical Research Council, Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9RP, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council, Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9RP, UK","institution_ids":["https://openalex.org/I162608824","https://openalex.org/I90344618"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111628871"],"corresponding_institution_ids":["https://openalex.org/I162608824"],"apc_list":{"value":3270,"currency":"USD","value_usd":3270},"apc_paid":null,"fwci":4.5779,"has_fulltext":false,"cited_by_count":101,"citation_normalized_percentile":{"value":0.94759576,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"162","issue":"2","first_page":"530","last_page":"538"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11827","display_name":"Polyomavirus and related diseases","score":0.4821999967098236,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11827","display_name":"Polyomavirus and related diseases","score":0.4821999967098236,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.4065000116825104,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.027499999850988388,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sv40-t-antigen","display_name":"SV40 T antigen","score":0.8460000157356262},{"id":"https://openalex.org/keywords/morphological-transformation","display_name":"morphological transformation","score":0.8029999732971191},{"id":"https://openalex.org/keywords/xanthine-guanine-phosphoribosyltransferase","display_name":"xanthine-guanine phosphoribosyltransferase","score":0.9369999766349792},{"id":"https://openalex.org/keywords/dominant-selectable-marker","display_name":"dominant selectable marker","score":0.9089999794960022},{"id":"https://openalex.org/keywords/plasmid-transfection","display_name":"plasmid transfection","score":0.8159999847412109},{"id":"https://openalex.org/keywords/cockayne-syndrome","display_name":"Cockayne syndrome","score":0.8270000219345093},{"id":"https://openalex.org/keywords/immunodeficiency","display_name":"immunodeficiency","score":0.6000000238418579},{"id":"https://openalex.org/keywords/cellular-senescence","display_name":"cellular senescence","score":0.8289999961853027},{"id":"https://openalex.org/keywords/crisis","display_name":"crisis","score":0.5619999766349792},{"id":"https://openalex.org/keywords/human-cell-lines","display_name":"human cell lines","score":0.5099999904632568}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9085036516189575},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.8516591787338257},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6661781072616577},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6526069045066833},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.5627052783966064},{"id":"https://openalex.org/C42411736","wikidata":"https://www.wikidata.org/wiki/Q1550327","display_name":"Selectable marker","level":4,"score":0.5337492227554321},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5236244797706604},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4934743642807007},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4840640127658844},{"id":"https://openalex.org/C2775971809","wikidata":"https://www.wikidata.org/wiki/Q7395063","display_name":"SV40 large T antigen","level":4,"score":0.4593075215816498},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.4465918242931366},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4429195523262024},{"id":"https://openalex.org/C2780381497","wikidata":"https://www.wikidata.org/wiki/Q463418","display_name":"Fibroblast","level":3,"score":0.4343675971031189},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4151475131511688},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.393758088350296},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3040933609008789}],"mesh":[{"descriptor_ui":"D000957","descriptor_name":"Antigens, Viral, Tumor","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002472","descriptor_name":"Cell Transformation, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003057","descriptor_name":"Cockayne Syndrome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003057","descriptor_name":"Cockayne Syndrome","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004392","descriptor_name":"Dwarfism","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010430","descriptor_name":"Pentosyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012867","descriptor_name":"Skin","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D013539","descriptor_name":"Simian virus 40","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0014-4827(86)90356-3","doi":"https://doi.org/10.1016/0014-4827(86)90356-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0014-4827(86)90356-3","pdf_url":null,"source":{"id":"https://openalex.org/S132505712","display_name":"Experimental Cell Research","issn_l":"0014-4827","issn":["0014-4827","1090-2422"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental Cell Research","raw_type":"journal-article"},{"id":"pmid:3002824","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3002824","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental cell research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8199999928474426}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W1499179356","https://openalex.org/W1519954836","https://openalex.org/W1643353036","https://openalex.org/W1965372133","https://openalex.org/W1967551846","https://openalex.org/W1981151145","https://openalex.org/W1990731481","https://openalex.org/W2004632795","https://openalex.org/W2018355080","https://openalex.org/W2063450941","https://openalex.org/W2068073829","https://openalex.org/W2405745947"],"related_works":["https://openalex.org/W2067067822","https://openalex.org/W2047911545","https://openalex.org/W1987704148","https://openalex.org/W2473549482","https://openalex.org/W2040394773","https://openalex.org/W1799722196","https://openalex.org/W2044202880","https://openalex.org/W2026355623","https://openalex.org/W2088072564","https://openalex.org/W1998998203"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}