{"id":"https://openalex.org/W2042751935","doi":"https://doi.org/10.1016/j.jim.2011.09.015","title":"Development and validation of a cell-based SEAP reporter assay for the detection of neutralizing antibodies against an anti-IL-13 therapeutic antibody","display_name":"Development and validation of a cell-based SEAP reporter assay for the detection of neutralizing antibodies against an anti-IL-13 therapeutic antibody","publication_year":2011,"publication_date":"2011-10-12","ids":{"openalex":"https://openalex.org/W2042751935","doi":"https://doi.org/10.1016/j.jim.2011.09.015","mag":"2042751935","pmid":"https://pubmed.ncbi.nlm.nih.gov/22001556"},"language":"en","primary_location":{"id":"doi:10.1016/j.jim.2011.09.015","doi":"https://doi.org/10.1016/j.jim.2011.09.015","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jim.2011.09.015","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075501994","display_name":"Karen Liao","orcid":"https://orcid.org/0000-0002-4685-7179","observed_orcids":["https://orcid.org/0000-0002-4685-7179"]},"institutions":[{"id":"https://openalex.org/I4210114143","display_name":"GlaxoSmithKline (United States)","ror":"https://ror.org/025vn3989","country_code":"US","type":"company","lineage":["https://openalex.org/I188950975","https://openalex.org/I4210114143"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karen Liao","raw_affiliation_strings":["Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States","institution_ids":["https://openalex.org/I4210114143"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110080565","display_name":"Dan Sikkema","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210114143","display_name":"GlaxoSmithKline (United States)","ror":"https://ror.org/025vn3989","country_code":"US","type":"company","lineage":["https://openalex.org/I188950975","https://openalex.org/I4210114143"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dan Sikkema","raw_affiliation_strings":["Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States","institution_ids":["https://openalex.org/I4210114143"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026190610","display_name":"Catherine Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210114143","display_name":"GlaxoSmithKline (United States)","ror":"https://ror.org/025vn3989","country_code":"US","type":"company","lineage":["https://openalex.org/I188950975","https://openalex.org/I4210114143"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Catherine Wang","raw_affiliation_strings":["Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States","institution_ids":["https://openalex.org/I4210114143"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047954240","display_name":"Keguan Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210114143","display_name":"GlaxoSmithKline (United States)","ror":"https://ror.org/025vn3989","country_code":"US","type":"company","lineage":["https://openalex.org/I188950975","https://openalex.org/I4210114143"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Keguan Chen","raw_affiliation_strings":["Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States","institution_ids":["https://openalex.org/I4210114143"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042823676","display_name":"Stephen L. DeWall","orcid":"https://orcid.org/0000-0002-8119-5748","observed_orcids":["https://orcid.org/0000-0002-8119-5748"]},"institutions":[{"id":"https://openalex.org/I4210114143","display_name":"GlaxoSmithKline (United States)","ror":"https://ror.org/025vn3989","country_code":"US","type":"company","lineage":["https://openalex.org/I188950975","https://openalex.org/I4210114143"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen DeWall","raw_affiliation_strings":["Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States","institution_ids":["https://openalex.org/I4210114143"]}]},{"author_position":"last","author":{"id":null,"display_name":"Thomas N. Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210114143","display_name":"GlaxoSmithKline (United States)","ror":"https://ror.org/025vn3989","country_code":"US","type":"company","lineage":["https://openalex.org/I188950975","https://openalex.org/I4210114143"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas N. Lee","raw_affiliation_strings":["Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Immunology, GlaxoSmithKline, King Of Prussia, PA 19406, United States","institution_ids":["https://openalex.org/I4210114143"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210114143"],"apc_list":{"value":2970,"currency":"USD","value_usd":2970},"apc_paid":null,"fwci":0.2633,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.53881261,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"375","issue":"1-2","first_page":"258","last_page":"263"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12255","display_name":"Biosimilars and Bioanalytical Methods","score":0.8906000256538391,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12255","display_name":"Biosimilars and Bioanalytical Methods","score":0.8906000256538391,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.08290000259876251,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10051","display_name":"Asthma and respiratory diseases","score":0.009200000204145908,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neutralizing-antibodies","display_name":"neutralizing antibodies","score":0.8209999799728394},{"id":"https://openalex.org/keywords/anti-il-13-monoclonal-antibody","display_name":"anti-IL-13 monoclonal antibody","score":0.9760000109672546},{"id":"https://openalex.org/keywords/il-13","display_name":"IL-13","score":0.8550000190734863},{"id":"https://openalex.org/keywords/cell-based-assays","display_name":"cell-based assays","score":0.8259999752044678},{"id":"https://openalex.org/keywords/assay-validation","display_name":"assay validation","score":0.7950000166893005},{"id":"https://openalex.org/keywords/asthma","display_name":"asthma","score":0.5210000276565552},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.800000011920929},{"id":"https://openalex.org/keywords/assay-sensitivity-and-specificity","display_name":"assay sensitivity and specificity","score":0.6309999823570251},{"id":"https://openalex.org/keywords/matrix-effects","display_name":"matrix effects","score":0.515999972820282}],"concepts":[{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.6791454553604126},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.62388676404953},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5813060998916626},{"id":"https://openalex.org/C2780367336","wikidata":"https://www.wikidata.org/wiki/Q4808618","display_name":"Assay sensitivity","level":3,"score":0.5157804489135742},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.4915793836116791},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.46809887886047363},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4239419400691986},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.4141950011253357},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3614981770515442},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.35013967752456665},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2569299340248108},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13186457753181458},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.08959195017814636},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.08493047952651978},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000469","descriptor_name":"Alkaline Phosphatase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000888","descriptor_name":"Antibodies, Anti-Idiotypic","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001249","descriptor_name":"Asthma","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003429","descriptor_name":"Cross Reactions","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007118","descriptor_name":"Immunoassay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015847","descriptor_name":"Interleukin-4","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D016898","descriptor_name":"Interferon-alpha","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018793","descriptor_name":"Interleukin-13","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D057134","descriptor_name":"Antibodies, Neutralizing","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jim.2011.09.015","doi":"https://doi.org/10.1016/j.jim.2011.09.015","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jim.2011.09.015","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},{"id":"pmid:22001556","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22001556","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9537000060081482,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W1967295501","https://openalex.org/W1983263771","https://openalex.org/W2008322431","https://openalex.org/W2070051070","https://openalex.org/W2090587541","https://openalex.org/W2108626100"],"related_works":["https://openalex.org/W2003941995","https://openalex.org/W2070051070","https://openalex.org/W2013948590","https://openalex.org/W2058135202","https://openalex.org/W2097191440","https://openalex.org/W1521025638","https://openalex.org/W1471362546","https://openalex.org/W3137839857","https://openalex.org/W7096260835","https://openalex.org/W2235250931"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}