{"id":"https://openalex.org/W2042054063","doi":"https://doi.org/10.1021/ja102257v","title":"Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens","display_name":"Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens","publication_year":2010,"publication_date":"2010-05-26","ids":{"openalex":"https://openalex.org/W2042054063","doi":"https://doi.org/10.1021/ja102257v","mag":"2042054063","pmid":"https://pubmed.ncbi.nlm.nih.gov/20504019","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2919334"},"language":"en","primary_location":{"id":"doi:10.1021/ja102257v","doi":"https://doi.org/10.1021/ja102257v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja102257v","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2919334","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042366783","display_name":"Jacob S. Yount","orcid":"https://orcid.org/0000-0002-6128-4575","observed_orcids":["https://orcid.org/0000-0002-6128-4575"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jacob S. Yount","raw_affiliation_strings":["Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794","institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055415786","display_name":"Lun K. Tsou","orcid":"https://orcid.org/0000-0002-1593-5226","observed_orcids":["https://orcid.org/0000-0002-1593-5226"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lun K. Tsou","raw_affiliation_strings":["Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794","institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035787351","display_name":"Paul D. Dossa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul D. Dossa","raw_affiliation_strings":["Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794","institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089271570","display_name":"Amy L. Kullas","orcid":"https://orcid.org/0000-0003-2429-4691","observed_orcids":["https://orcid.org/0000-0003-2429-4691"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amy L. Kullas","raw_affiliation_strings":["Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794","institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011655832","display_name":"Adrianus W. M. van der Velden","orcid":"https://orcid.org/0000-0003-3006-0306","observed_orcids":["https://orcid.org/0000-0003-3006-0306"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adrianus W. M. van der Velden","raw_affiliation_strings":["Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794","institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088099956","display_name":"Howard C. Hang","orcid":"https://orcid.org/0000-0003-4053-5547","observed_orcids":["https://orcid.org/0000-0003-4053-5547","https://orcid.org/0000-0003-3373-7140"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Howard C. Hang","raw_affiliation_strings":["Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065 and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794","institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5088099956"],"corresponding_institution_ids":["https://openalex.org/I59553526","https://openalex.org/I83399316"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.4409,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.78867216,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"132","issue":"24","first_page":"8244","last_page":"8245"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.4984999895095825,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.4984999895095825,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.2371000051498413,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.059700001031160355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacterial-effectors","display_name":"bacterial effector proteins","score":0.6330000162124634},{"id":"https://openalex.org/keywords/carboxypeptidase-g2","display_name":"carboxypeptidase G2","score":0.9779999852180481},{"id":"https://openalex.org/keywords/reporter-system","display_name":"reporter system","score":0.6179999709129333},{"id":"https://openalex.org/keywords/fluorescence-detection","display_name":"fluorescence detection","score":0.7549999952316284},{"id":"https://openalex.org/keywords/salmonella-typhimurium","display_name":"Salmonella typhimurium","score":0.9739999771118164},{"id":"https://openalex.org/keywords/gram-negative-pathogens","display_name":"Gram-negative pathogens","score":0.7250000238418579},{"id":"https://openalex.org/keywords/antibiotic-targets","display_name":"antibiotic targets","score":0.7990000247955322},{"id":"https://openalex.org/keywords/high-throughput-assay","display_name":"high-throughput assay","score":0.7300000190734863}],"concepts":[{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.8640279173851013},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8231438398361206},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.7901793718338013},{"id":"https://openalex.org/C2781065037","wikidata":"https://www.wikidata.org/wiki/Q150839","display_name":"Salmonella","level":3,"score":0.6431863903999329},{"id":"https://openalex.org/C130211564","wikidata":"https://www.wikidata.org/wiki/Q21199165","display_name":"Type three secretion system","level":4,"score":0.5448808670043945},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.49910974502563477},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4402995705604553},{"id":"https://openalex.org/C156399914","wikidata":"https://www.wikidata.org/wiki/Q7444454","display_name":"Secretory protein","level":3,"score":0.4326637387275696},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.430597186088562},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.386226624250412},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.11526808142662048},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.0746796727180481},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07217398285865784},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003116","descriptor_name":"Color","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011623","descriptor_name":"gamma-Glutamyl Hydrolase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019779","descriptor_name":"Salmonella enterica","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1021/ja102257v","doi":"https://doi.org/10.1021/ja102257v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja102257v","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:20504019","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20504019","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:2118602","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2919334","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:2919334","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2919334","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Am Chem Soc","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:2118602","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2919334","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8733888550","display_name":"Northeast Biodefense Center","funder_award_id":"U54AI057158","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1965933688","https://openalex.org/W1971204318","https://openalex.org/W1977871291","https://openalex.org/W1982019125","https://openalex.org/W1993933593","https://openalex.org/W2000707384","https://openalex.org/W2015150314","https://openalex.org/W2021071631","https://openalex.org/W2024180691","https://openalex.org/W2049906028","https://openalex.org/W2071999738","https://openalex.org/W2127318266","https://openalex.org/W2158295903","https://openalex.org/W2162051865"],"related_works":["https://openalex.org/W2148663046","https://openalex.org/W4411704884","https://openalex.org/W1978146003","https://openalex.org/W3102147795","https://openalex.org/W196460864","https://openalex.org/W2163670463","https://openalex.org/W2159643690","https://openalex.org/W4245863404","https://openalex.org/W2750933264","https://openalex.org/W2021694299"],"abstract_inverted_index":{"Type":[0],"III":[1,40,69],"protein":[2,27,41,54,70],"secretion":[3,42,57,71],"is":[4],"essential":[5],"for":[6,18,33,52,66],"many":[7],"gram-negative":[8],"bacterial":[9,26],"infections":[10],"of":[11,38],"host":[12],"cells":[13],"and":[14,35,56,63],"an":[15],"attractive":[16],"target":[17],"new":[19],"antibacterial":[20],"drugs.":[21],"Here,":[22],"we":[23],"describe":[24],"a":[25,49,61],"effector-carboxypeptidase":[28],"G2":[29],"(CPG2)":[30],"reporter":[31],"system":[32,47],"fluorescence":[34],"visible":[36],"detection":[37],"type":[39,68],"in":[43],"Salmonella":[44],"typhimurium.":[45],"This":[46],"provides":[48],"general":[50],"method":[51],"measuring":[53],"expression":[55],"as":[58,60],"well":[59],"high-throughput":[62],"quantitative":[64],"assay":[65],"analyzing":[67],"inhibitors.":[72]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}
