{"id":"https://openalex.org/W2041111684","doi":"https://doi.org/10.1099/00221287-134-7-1779","title":"Construction of Multicopy Expression Vectors for Regulated Over-production of Proteins in Klebsiella pneumoniae and Other Enteric Bacteria","display_name":"Construction of Multicopy Expression Vectors for Regulated Over-production of Proteins in Klebsiella pneumoniae and Other Enteric Bacteria","publication_year":1988,"publication_date":"1988-07-01","ids":{"openalex":"https://openalex.org/W2041111684","doi":"https://doi.org/10.1099/00221287-134-7-1779","mag":"2041111684","pmid":"https://pubmed.ncbi.nlm.nih.gov/3073188"},"language":"en","primary_location":{"id":"doi:10.1099/00221287-134-7-1779","doi":"https://doi.org/10.1099/00221287-134-7-1779","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-134-7-1779","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061563366","display_name":"Diethelm Kleiner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"D. Kleiner","raw_affiliation_strings":["AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton, UK","AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton, UK","institution_ids":["https://openalex.org/I162608824"]},{"raw_affiliation_string":"AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK","institution_ids":["https://openalex.org/I162608824"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003512996","display_name":"Wyatt Paul","orcid":"https://orcid.org/0009-0005-2154-8280","observed_orcids":["https://orcid.org/0009-0005-2154-8280"]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"W. Paul","raw_affiliation_strings":["AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK","institution_ids":["https://openalex.org/I162608824"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111045045","display_name":"M. J. Merrick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162608824","display_name":"University of Sussex","ror":"https://ror.org/00ayhx656","country_code":"GB","type":"education","lineage":["https://openalex.org/I162608824"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"M. J. Merrick","raw_affiliation_strings":["AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK","institution_ids":["https://openalex.org/I162608824"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I162608824"],"apc_list":null,"apc_paid":null,"fwci":3.0832,"has_fulltext":false,"cited_by_count":114,"citation_normalized_percentile":{"value":0.91693478,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"134","issue":"7","first_page":"1779","last_page":"1784"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9785000085830688,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9785000085830688,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.014499999582767487,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-overexpression","display_name":"protein overexpression","score":0.8040000200271606},{"id":"https://openalex.org/keywords/klebsiella-pneumoniae","display_name":"Klebsiella pneumoniae","score":0.996999979019165},{"id":"https://openalex.org/keywords/enteric-bacteria","display_name":"enteric bacteria","score":0.984000027179718},{"id":"https://openalex.org/keywords/tac-promoter","display_name":"tac promoter","score":0.8130000233650208},{"id":"https://openalex.org/keywords/lac-repressor","display_name":"lac repressor","score":0.7940000295639038},{"id":"https://openalex.org/keywords/gene-expression-regulation","display_name":"gene expression regulation","score":0.6690000295639038},{"id":"https://openalex.org/keywords/selectable-marker","display_name":"selectable marker","score":0.7160000205039978},{"id":"https://openalex.org/keywords/kanamycin-resistance","display_name":"kanamycin resistance","score":0.8040000200271606},{"id":"https://openalex.org/keywords/chloramphenicol-resistance","display_name":"chloramphenicol resistance","score":0.9919999837875366},{"id":"https://openalex.org/keywords/ampicillin-resistance","display_name":"ampicillin resistance","score":0.9259999990463257},{"id":"https://openalex.org/keywords/beta-lactamase","display_name":"beta-lactamase","score":0.949999988079071}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7470254302024841},{"id":"https://openalex.org/C2777058267","wikidata":"https://www.wikidata.org/wiki/Q132592","display_name":"Klebsiella pneumoniae","level":4,"score":0.7405933737754822},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.7372851371765137},{"id":"https://openalex.org/C2780920689","wikidata":"https://www.wikidata.org/wiki/Q27094615","display_name":"Kanamycin","level":3,"score":0.6394979953765869},{"id":"https://openalex.org/C113629113","wikidata":"https://www.wikidata.org/wiki/Q4345509","display_name":"Lac repressor","level":5,"score":0.6030207872390747},{"id":"https://openalex.org/C42411736","wikidata":"https://www.wikidata.org/wiki/Q1550327","display_name":"Selectable marker","level":4,"score":0.5578351616859436},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5371876955032349},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5270217657089233},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4806150794029236},{"id":"https://openalex.org/C107618985","wikidata":"https://www.wikidata.org/wiki/Q358963","display_name":"Multiple cloning site","level":5,"score":0.4720505177974701},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.46096110343933105},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.41664204001426697},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.414113312959671},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.37580469250679016},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.37423670291900635},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.18192055821418762}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007711","descriptor_name":"Klebsiella pneumoniae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007711","descriptor_name":"Klebsiella pneumoniae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1099/00221287-134-7-1779","doi":"https://doi.org/10.1099/00221287-134-7-1779","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-134-7-1779","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},{"id":"pmid:3073188","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3073188","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of general microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1540695101","https://openalex.org/W1974072101","https://openalex.org/W1984640298","https://openalex.org/W2016806244","https://openalex.org/W2022064097","https://openalex.org/W2032776824","https://openalex.org/W2043845627","https://openalex.org/W2054887464","https://openalex.org/W2064112457","https://openalex.org/W2068004788","https://openalex.org/W2075330450","https://openalex.org/W2141653906","https://openalex.org/W2144634347"],"related_works":["https://openalex.org/W2068004788","https://openalex.org/W2111836375","https://openalex.org/W2065335949","https://openalex.org/W1994594395","https://openalex.org/W2528244929","https://openalex.org/W2090999973","https://openalex.org/W2047957361","https://openalex.org/W1965157361","https://openalex.org/W4403909960","https://openalex.org/W1824120126"],"abstract_inverted_index":{"A":[0],"number":[1],"of":[2,11,41,54,62,93,112,126],"expression":[3],"vectors":[4,72],"have":[5],"been":[6],"constructed":[7,99],"to":[8,68,87,120],"allow":[9],"over-production":[10,61],"selected":[12],"gene":[13,32,55],"products":[14],"in":[15,79,95,116],"Klebsiella":[16],"pneumoniae":[17,118],"and":[18],"other":[19],"enteric":[20],"bacteria.":[21],"The":[22,71],"plasmids":[23],"use":[24],"the":[25,38,42,47,74,82,88,91,109,121],"strong":[26],"hybrid":[27],"trp-lac":[28],"(tac)":[29],"promoter":[30],"for":[31,60],"expression,":[33,56],"which":[34,57,64,80,113],"is":[35,58],"regulated":[36],"by":[37],"lacIQ":[39],"allele":[40],"lac":[43],"repressor":[44],"carried":[45],"on":[46],"vector.":[48],"This":[49],"provides":[50],"very":[51],"tight":[52],"regulation":[53],"important":[59],"proteins":[63],"may":[65],"be":[66],"detrimental":[67],"cell":[69],"growth.":[70],"carry":[73],"standard":[75],"mp18":[76],"cloning":[77],"nest":[78],"all":[81],"restriction":[83],"sites":[84],"are":[85,114],"unique":[86],"plasmid":[89],"(with":[90],"exception":[92],"EcoRI":[94],"pDK7).":[96],"Derivatives":[97],"were":[98],"carrying":[100],"kanamycin,":[101],"chloramphenicol":[102],"or":[103],"ampicillin":[104],"resistance":[105],"as":[106],"selectable":[107],"markers,":[108],"first":[110],"two":[111],"advantageous":[115],"K.":[117],"due":[119],"high":[122],"inherent":[123],"beta-lactamase":[124],"activity":[125],"this":[127],"organism.":[128]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
