{"id":"https://openalex.org/W2040948512","doi":"https://doi.org/10.1034/j.1600-0765.2003.02006.x","title":"Combined TUNEL and TRAP methods suggest that apoptotic bone cells are inside vacuoles of alveolar bone osteoclasts in young rats","display_name":"Combined TUNEL and TRAP methods suggest that apoptotic bone cells are inside vacuoles of alveolar bone osteoclasts in young rats","publication_year":2003,"publication_date":"2003-02-28","ids":{"openalex":"https://openalex.org/W2040948512","doi":"https://doi.org/10.1034/j.1600-0765.2003.02006.x","mag":"2040948512","pmid":"https://pubmed.ncbi.nlm.nih.gov/12608919"},"language":"en","primary_location":{"id":"doi:10.1034/j.1600-0765.2003.02006.x","doi":"https://doi.org/10.1034/j.1600-0765.2003.02006.x","is_oa":false,"landing_page_url":"https://doi.org/10.1034/j.1600-0765.2003.02006.x","pdf_url":null,"source":{"id":"https://openalex.org/S195000414","display_name":"Journal of Periodontal Research","issn_l":"0022-3484","issn":["0022-3484","1600-0765"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Periodontal Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014936138","display_name":"Paulo S\u00e9rgio Cerri","orcid":"https://orcid.org/0000-0001-5756-5828","observed_orcids":["https://orcid.org/0000-0001-5756-5828"]},"institutions":[{"id":"https://openalex.org/I879563668","display_name":"Universidade Estadual Paulista (Unesp)","ror":"https://ror.org/00987cb86","country_code":"BR","type":"education","lineage":["https://openalex.org/I879563668"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"P. S. Cerri","raw_affiliation_strings":["Department of Morphology, S\u00e3o Paulo State University (UNESP-Araraquara) SP, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Morphology, S\u00e3o Paulo State University (UNESP-Araraquara) SP, Brazil","institution_ids":["https://openalex.org/I879563668"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069282138","display_name":"Fernanda Boabaid","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I88273585","display_name":"Universidade Federal de S\u00e3o Paulo","ror":"https://ror.org/02k5swt12","country_code":"BR","type":"education","lineage":["https://openalex.org/I88273585"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"F. Boabaid","raw_affiliation_strings":["Department of Morphology, Federal University of Sa\u0303o Paulo (UNIFESP), S.P., Brazil","Department of Morphology, Federal University of S\u00e3o Paulo (UNIFESP), S.P., Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Morphology, Federal University of Sa\u0303o Paulo (UNIFESP), S.P., Brazil","institution_ids":["https://openalex.org/I88273585"]},{"raw_affiliation_string":"Department of Morphology, Federal University of S\u00e3o Paulo (UNIFESP), S.P., Brazil","institution_ids":["https://openalex.org/I88273585"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062472666","display_name":"E. Katchburian","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I88273585","display_name":"Universidade Federal de S\u00e3o Paulo","ror":"https://ror.org/02k5swt12","country_code":"BR","type":"education","lineage":["https://openalex.org/I88273585"]}],"countries":["BR"],"is_corresponding":true,"raw_author_name":"E. Katchburian","raw_affiliation_strings":["Department of Morphology, Federal University of Sa\u0303o Paulo (UNIFESP), S.P., Brazil","Department of Morphology, Federal University of S\u00e3o Paulo (UNIFESP), S.P., Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Morphology, Federal University of Sa\u0303o Paulo (UNIFESP), S.P., Brazil","institution_ids":["https://openalex.org/I88273585"]},{"raw_affiliation_string":"Department of Morphology, Federal University of S\u00e3o Paulo (UNIFESP), S.P., Brazil","institution_ids":["https://openalex.org/I88273585"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5062472666"],"corresponding_institution_ids":["https://openalex.org/I88273585"],"apc_list":{"value":4880,"currency":"USD","value_usd":4880},"apc_paid":null,"fwci":0.7543,"has_fulltext":false,"cited_by_count":63,"citation_normalized_percentile":{"value":0.64353548,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"38","issue":"2","first_page":"223","last_page":"226"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11219","display_name":"Bone Metabolism and Diseases","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11219","display_name":"Bone Metabolism and Diseases","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12672","display_name":"Phagocytosis and Immune Regulation","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/osteoclasts","display_name":"osteoclasts","score":0.9649999737739563},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8569999933242798},{"id":"https://openalex.org/keywords/alveolar-bone","display_name":"alveolar bone","score":0.7310000061988831},{"id":"https://openalex.org/keywords/bone-remodeling","display_name":"bone remodeling","score":0.8389999866485596},{"id":"https://openalex.org/keywords/osteoblasts","display_name":"osteoblasts","score":0.828000009059906},{"id":"https://openalex.org/keywords/osteocytes","display_name":"osteocytes","score":0.8849999904632568},{"id":"https://openalex.org/keywords/vacuoles","display_name":"vacuoles","score":0.824999988079071},{"id":"https://openalex.org/keywords/tunel-assay","display_name":"TUNEL","score":0.7570000290870667},{"id":"https://openalex.org/keywords/traps","display_name":"traps","score":0.777999997138977},{"id":"https://openalex.org/keywords/bone-resorption","display_name":"bone resorption","score":0.6959999799728394}],"concepts":[{"id":"https://openalex.org/C196795494","wikidata":"https://www.wikidata.org/wiki/Q1624817","display_name":"TUNEL assay","level":3,"score":0.8094120025634766},{"id":"https://openalex.org/C2780973866","wikidata":"https://www.wikidata.org/wiki/Q913236","display_name":"Osteocyte","level":4,"score":0.7103927135467529},{"id":"https://openalex.org/C151279780","wikidata":"https://www.wikidata.org/wiki/Q143588","display_name":"Dental alveolus","level":2,"score":0.6530194878578186},{"id":"https://openalex.org/C673006","wikidata":"https://www.wikidata.org/wiki/Q4941581","display_name":"Bone resorption","level":2,"score":0.652585506439209},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6009218096733093},{"id":"https://openalex.org/C21547065","wikidata":"https://www.wikidata.org/wiki/Q418243","display_name":"Acid phosphatase","level":3,"score":0.5534986853599548},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5148662328720093},{"id":"https://openalex.org/C2776033226","wikidata":"https://www.wikidata.org/wiki/Q828410","display_name":"Osteoclast","level":3,"score":0.5012357234954834},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.49257975816726685},{"id":"https://openalex.org/C133605539","wikidata":"https://www.wikidata.org/wiki/Q913236","display_name":"Bone cell","level":2,"score":0.4852721393108368},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4689492881298065},{"id":"https://openalex.org/C170033053","wikidata":"https://www.wikidata.org/wiki/Q952019","display_name":"Bone remodeling","level":2,"score":0.4337151348590851},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3833441436290741},{"id":"https://openalex.org/C2778260815","wikidata":"https://www.wikidata.org/wiki/Q917177","display_name":"Osteoblast","level":3,"score":0.23587313294410706},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.2111257016658783},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.18999835848808289},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1749270260334015},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.16959986090660095},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08500388264656067},{"id":"https://openalex.org/C199343813","wikidata":"https://www.wikidata.org/wiki/Q12128","display_name":"Dentistry","level":1,"score":0.0766003429889679},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000071681","descriptor_name":"Tartrate-Resistant Acid Phosphatase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000135","descriptor_name":"Acid Phosphatase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D000539","descriptor_name":"Alveolar Process","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001862","descriptor_name":"Bone Resorption","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D004396","descriptor_name":"Coloring Agents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D010010","descriptor_name":"Osteoclasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D010011","descriptor_name":"Osteocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D014617","descriptor_name":"Vacuoles","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D014617","descriptor_name":"Vacuoles","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016723","descriptor_name":"Bone Remodeling","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D017208","descriptor_name":"Rats, Wistar","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020287","descriptor_name":"In Situ Nick-End Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1034/j.1600-0765.2003.02006.x","doi":"https://doi.org/10.1034/j.1600-0765.2003.02006.x","is_oa":false,"landing_page_url":"https://doi.org/10.1034/j.1600-0765.2003.02006.x","pdf_url":null,"source":{"id":"https://openalex.org/S195000414","display_name":"Journal of Periodontal Research","issn_l":"0022-3484","issn":["0022-3484","1600-0765"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Periodontal Research","raw_type":"journal-article"},{"id":"pmid:12608919","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12608919","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of periodontal research","raw_type":"Journal Article"},{"id":"pmh:oai:acervodigital.unesp.br:11449/38050","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/11449/38050","pdf_url":null,"source":{"id":"https://openalex.org/S4306400256","display_name":"Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210115194","host_organization_name":"Funda\u00e7\u00e3o para o Desenvolvimento da UNESP","host_organization_lineage":["https://openalex.org/I4210115194"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"outro"},{"id":"pmh:oai:repositorio.unesp.br:11449/38050","doi":null,"is_oa":false,"landing_page_url":"http://doi.org/10.1034/j.1600-0765.2003.02006.x","pdf_url":null,"source":{"id":"https://openalex.org/S4377196277","display_name":"UNESP Institutional Repository (S\u00e3o Paulo State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I879563668","host_organization_name":"Universidade Estadual Paulista (Unesp)","host_organization_lineage":["https://openalex.org/I879563668"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:repositorio.unifesp.br:11600/27196","doi":null,"is_oa":false,"landing_page_url":"http://repositorio.unifesp.br/handle/11600/27196","pdf_url":null,"source":{"id":"https://openalex.org/S4377196380","display_name":"UNIFESP Institutional Repository (Universidade Federal de S\u00e3o Paulo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I88273585","host_organization_name":"Universidade Federal de S\u00e3o Paulo","host_organization_lineage":["https://openalex.org/I88273585"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Artigo"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W71446123","https://openalex.org/W1968807231","https://openalex.org/W1976761657","https://openalex.org/W1986942955","https://openalex.org/W2002315967","https://openalex.org/W2005128372","https://openalex.org/W2008698339","https://openalex.org/W2018330233","https://openalex.org/W2020548763","https://openalex.org/W2020734792","https://openalex.org/W2027215054","https://openalex.org/W2032776924","https://openalex.org/W2036191411","https://openalex.org/W2038622939","https://openalex.org/W2066488281","https://openalex.org/W2070347347","https://openalex.org/W2075292460","https://openalex.org/W2087229400","https://openalex.org/W2095402053","https://openalex.org/W2138302361","https://openalex.org/W2161508058","https://openalex.org/W2161690532","https://openalex.org/W2166511996","https://openalex.org/W2169158043"],"related_works":["https://openalex.org/W2005128372","https://openalex.org/W2095402053","https://openalex.org/W2005950537","https://openalex.org/W2804297149","https://openalex.org/W2390103567","https://openalex.org/W2135444604","https://openalex.org/W1522615306","https://openalex.org/W2018330233","https://openalex.org/W2003995422","https://openalex.org/W1976873827"],"abstract_inverted_index":{"Although":[0],"it":[1,71],"is":[2,63,72],"generally":[3],"accepted":[4],"that":[5,14,47,149,173],"osteoclasts":[6,42,116,137,152,176],"breakdown":[7],"and":[8,36,41,101,115,124,138],"resorb":[9],"bone":[10,66,83,140,144,175,183],"matrix,":[11],"the":[12,64,90,102,109,118,128,171],"possibility":[13],"they":[15],"may":[16],"also":[17],"be":[18],"able":[19,178],"to":[20,50,179],"engulf":[21],"apoptotic":[22,113,139,159,182],"osteoblasts/lining":[23,164],"cells":[24,33,40,114,141,165],"and/or":[25,34,166],"osteocytes":[26,35],"remains":[27],"controversial.":[28],"Apoptosis":[29],"of":[30,67,79,84,112,134],"osteoblasts/":[31],"lining":[32],"interactions":[37],"between":[38,136],"these":[39,61],"are":[43,48,177],"extremely":[44],"rapid":[45],"events":[46,62],"difficult":[49],"observe":[51],"in":[52,56,117,127],"vivo.":[53],"A":[54],"suitable":[55],"vivo":[57],"model":[58],"for":[59,108],"studying":[60],"alveolar":[65,82,143,174],"young":[68,85],"rats":[69,86],"because":[70],"continuously":[73],"undergoing":[74],"intense":[75],"resorption/remodeling.":[76],"Thus,":[77],"sections":[78],"aldehyde":[80],"fixed":[81],"were":[87],"stained":[88],"by":[89],"combined":[91,122],"terminal":[92],"deoxynucleotidyl":[93],"transferase-mediated":[94],"dUTP":[95],"nick":[96],"end":[97],"labeling":[98],"(TUNEL)":[99],"method":[100,107],"tartrate-resistant":[103],"acid":[104],"phosphatase":[105],"(TRAP)":[106],"simultaneous":[110],"visualization":[111,133],"same":[119,129],"section.":[120],"The":[121],"TUNEL":[123,157],"TRAP":[125],"reactions,":[126],"section,":[130],"greatly":[131],"facilitated":[132],"relationship":[135],"during":[142],"remodeling.":[145],"Our":[146],"results":[147,169],"showed":[148],"several":[150],"TRAP-positive":[151],"exhibited":[153],"large":[154],"vacuoles":[155],"containing":[156],"positive":[158],"structures,":[160],"probably":[161],"derived":[162],"from":[163],"osteocytes.":[167],"These":[168],"support":[170],"idea":[172],"internalize":[180],"dying":[181],"cells.":[184]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}