{"id":"https://openalex.org/W2040911333","doi":"https://doi.org/10.1016/s0014-5793(03)00580-5","title":"Mutational analysis of the Mycobacterium tuberculosis Rv1625c adenylyl cyclase: residues that confer nucleotide specificity contribute to dimerization","display_name":"Mutational analysis of the Mycobacterium tuberculosis Rv1625c adenylyl cyclase: residues that confer nucleotide specificity contribute to dimerization","publication_year":2003,"publication_date":"2003-05-28","ids":{"openalex":"https://openalex.org/W2040911333","doi":"https://doi.org/10.1016/s0014-5793(03)00580-5","mag":"2040911333","pmid":"https://pubmed.ncbi.nlm.nih.gov/12804785"},"language":"en","primary_location":{"id":"doi:10.1016/s0014-5793(03)00580-5","doi":"https://doi.org/10.1016/s0014-5793(03)00580-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0014-5793(03)00580-5","pdf_url":null,"source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030580990","display_name":"Avinash R. Shenoy","orcid":"https://orcid.org/0000-0001-6228-9303","observed_orcids":["https://orcid.org/0000-0001-6228-9303"]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Avinash R Shenoy","raw_affiliation_strings":["Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India","institution_ids":["https://openalex.org/I59270414"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076920718","display_name":"Narayanaswamy Srinivasan","orcid":"https://orcid.org/0000-0002-6566-6239","observed_orcids":["https://orcid.org/0000-0002-6566-6239"]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"N Srinivasan","raw_affiliation_strings":["Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India","institution_ids":["https://openalex.org/I59270414"]}]},{"author_position":"middle","author":{"id":null,"display_name":"M Subramaniam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"M Subramaniam","raw_affiliation_strings":["Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India","institution_ids":["https://openalex.org/I59270414"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027255071","display_name":"Sandhya Srikant Visweswariah","orcid":"https://orcid.org/0000-0003-2786-7344","observed_orcids":["https://orcid.org/0000-0003-2786-7344"]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Sandhya S Visweswariah","raw_affiliation_strings":["Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India","institution_ids":["https://openalex.org/I59270414"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5027255071"],"corresponding_institution_ids":["https://openalex.org/I59270414"],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":3.2863,"has_fulltext":false,"cited_by_count":33,"citation_normalized_percentile":{"value":0.92901218,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"545","issue":"2-3","first_page":"253","last_page":"259"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.2856000065803528,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.2856000065803528,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.1737000048160553,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.1729000061750412,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycobacterium-tuberculosis","display_name":"Mycobacterium tuberculosis","score":0.9599999785423279},{"id":"https://openalex.org/keywords/adenylate-cyclase","display_name":"adenylate cyclase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/nucleotide-specificity","display_name":"nucleotide specificity","score":0.824999988079071},{"id":"https://openalex.org/keywords/homodimerization","display_name":"homodimerization","score":0.7689999938011169},{"id":"https://openalex.org/keywords/dimer-interface","display_name":"dimer interface","score":0.8989999890327454},{"id":"https://openalex.org/keywords/oligomeric-state","display_name":"oligomeric state","score":0.8550000190734863},{"id":"https://openalex.org/keywords/catalytic-domain","display_name":"catalytic domain","score":0.8159999847412109},{"id":"https://openalex.org/keywords/computational-modeling","display_name":"computational modeling","score":0.5820000171661377}],"concepts":[{"id":"https://openalex.org/C2779178603","wikidata":"https://www.wikidata.org/wiki/Q356257","display_name":"Adenylyl cyclase","level":3,"score":0.9117064476013184},{"id":"https://openalex.org/C69101867","wikidata":"https://www.wikidata.org/wiki/Q17709777","display_name":"ADCY9","level":4,"score":0.8194770812988281},{"id":"https://openalex.org/C2751546","wikidata":"https://www.wikidata.org/wiki/Q18042074","display_name":"ADCY10","level":5,"score":0.8062303066253662},{"id":"https://openalex.org/C2780069284","wikidata":"https://www.wikidata.org/wiki/Q5198121","display_name":"Cyclase","level":3,"score":0.6693639755249023},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6580044627189636},{"id":"https://openalex.org/C117794106","wikidata":"https://www.wikidata.org/wiki/Q17709659","display_name":"ADCY6","level":5,"score":0.6060793399810791},{"id":"https://openalex.org/C9140185","wikidata":"https://www.wikidata.org/wiki/Q17709649","display_name":"ADCY3","level":5,"score":0.5895499587059021},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5838810205459595},{"id":"https://openalex.org/C31519591","wikidata":"https://www.wikidata.org/wiki/Q18026719","display_name":"GUCY2D","level":5,"score":0.5475120544433594},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5343261361122131},{"id":"https://openalex.org/C176303035","wikidata":"https://www.wikidata.org/wiki/Q5513929","display_name":"Gs alpha subunit","level":4,"score":0.454372376203537},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37032878398895264},{"id":"https://openalex.org/C13121076","wikidata":"https://www.wikidata.org/wiki/Q1552797","display_name":"Guanylate cyclase 2C","level":4,"score":0.31751102209091187}],"mesh":[{"descriptor_ui":"D000262","descriptor_name":"Adenylyl Cyclases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000262","descriptor_name":"Adenylyl Cyclases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000262","descriptor_name":"Adenylyl Cyclases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009169","descriptor_name":"Mycobacterium tuberculosis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019281","descriptor_name":"Dimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0014-5793(03)00580-5","doi":"https://doi.org/10.1016/s0014-5793(03)00580-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0014-5793(03)00580-5","pdf_url":null,"source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:12804785","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12804785","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"},{"id":"pmh:oai:eprints.iisc.ac.in:12247","doi":null,"is_oa":false,"landing_page_url":"http://eprints.iisc.ac.in/12247/1/sdarticle.pdf27.pdf36.pdf","pdf_url":"http://eprints.iisc.ac.in/12247/1/sdarticle.pdf27.pdf36.pdf","source":{"id":"https://openalex.org/S4377196309","display_name":"NOT FOUND REPOSITORY (Indian Institute of Science Bangalore)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I59270414","host_organization_name":"Indian Institute of Science Bangalore","host_organization_lineage":["https://openalex.org/I59270414"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1525055187","https://openalex.org/W1894685331","https://openalex.org/W1966316616","https://openalex.org/W1966679395","https://openalex.org/W1968573434","https://openalex.org/W1972038609","https://openalex.org/W1976402681","https://openalex.org/W1977922844","https://openalex.org/W1979046104","https://openalex.org/W1983094993","https://openalex.org/W1990591017","https://openalex.org/W1996213694","https://openalex.org/W2010730039","https://openalex.org/W2015860873","https://openalex.org/W2029895712","https://openalex.org/W2038470872","https://openalex.org/W2038936167","https://openalex.org/W2049521571","https://openalex.org/W2053449148","https://openalex.org/W2054733991","https://openalex.org/W2097382368","https://openalex.org/W2101871915","https://openalex.org/W2125209476","https://openalex.org/W2139919097","https://openalex.org/W2158023121","https://openalex.org/W2158997145","https://openalex.org/W2163181687","https://openalex.org/W2165055104","https://openalex.org/W2799449195"],"related_works":["https://openalex.org/W2206923713","https://openalex.org/W2079415904","https://openalex.org/W2004713793","https://openalex.org/W2125883642","https://openalex.org/W1996213694","https://openalex.org/W1986679035","https://openalex.org/W7095238017","https://openalex.org/W2038470872","https://openalex.org/W6947628478","https://openalex.org/W14486013"],"abstract_inverted_index":{"The":[0,16],"mycobacterial":[1],"Rv1625c":[2],"gene":[3],"product":[4],"is":[5],"an":[6],"adenylyl":[7,60,93],"cyclase":[8,61,94],"with":[9],"sequence":[10],"similarity":[11],"to":[12,41,48,52,98],"the":[13,20,34,50,74,80,85,101],"mammalian":[14,103],"enzymes.":[15,104],"catalytic":[17,88],"domain":[18],"of":[19,38,90,100],"enzyme":[21,51],"forms":[22],"a":[23,53],"homodimer":[24],"and":[25,63,87],"residues":[26,40],"specifying":[27],"adenosine":[28],"triphosphate":[29],"(ATP)":[30],"specificity":[31],"lie":[32],"at":[33],"dimer":[35,75],"interface.":[36],"Mutation":[37],"these":[39],"those":[42,99],"present":[43],"in":[44,73,96],"guanylyl":[45,54],"cyclases":[46],"failed":[47],"convert":[49],"cyclase,":[55],"but":[56],"dramatically":[57],"reduced":[58],"its":[59,65],"activity":[62],"altered":[64],"oligomeric":[66],"state.":[67],"Computational":[68],"modeling":[69],"revealed":[70],"subtle":[71],"differences":[72],"interface":[76],"that":[77,84],"could":[78],"explain":[79],"biochemical":[81],"data,":[82],"suggesting":[83],"structural":[86],"features":[89],"this":[91],"homodimeric":[92],"are":[95],"contrast":[97],"heterodimeric":[102]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}