{"id":"https://openalex.org/W2039737101","doi":"https://doi.org/10.1042/0264-6021:3510697","title":"Analysis of a nucleotide-binding site of 5-lipoxygenase by affinity labelling: binding characteristics and amino acid sequences","display_name":"Analysis of a nucleotide-binding site of 5-lipoxygenase by affinity labelling: binding characteristics and amino acid sequences","publication_year":2000,"publication_date":"2000-10-24","ids":{"openalex":"https://openalex.org/W2039737101","doi":"https://doi.org/10.1042/0264-6021:3510697","mag":"2039737101","pmid":"https://pubmed.ncbi.nlm.nih.gov/11042125","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1221410"},"language":"en","primary_location":{"id":"doi:10.1042/0264-6021:3510697","doi":"https://doi.org/10.1042/0264-6021:3510697","is_oa":false,"landing_page_url":"https://doi.org/10.1042/0264-6021:3510697","pdf_url":null,"source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/351/3/697/736957/bj3510697.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100685569","display_name":"Yingyi Zhang","orcid":"https://orcid.org/0000-0003-1020-7964","observed_orcids":["https://orcid.org/0000-0003-1020-7964"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ying-Yi ZHANG","raw_affiliation_strings":["Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, U.S.A","institution_ids":["https://openalex.org/I111088046"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056909109","display_name":"Tove Hammarberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Tove HAMMARBERG","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082257614","display_name":"Olof R\u00e5dmark","orcid":"https://orcid.org/0000-0002-3424-3890","observed_orcids":["https://orcid.org/0000-0002-3424-3890"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Olof RADMARK","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112759205","display_name":"Bengt O. Samuelsson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Bengt SAMUELSSON","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086242556","display_name":"Carol F. Ng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286694","display_name":"University of Medicine and Dentistry of New Jersey","ror":"https://ror.org/02tvvmk84","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4405286694"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carol F. NG","raw_affiliation_strings":["Department of Cell Biology, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, U.S.A","institution_ids":["https://openalex.org/I4405286694"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032705392","display_name":"Colin D. Funk","orcid":"https://orcid.org/0000-0001-7029-4233","observed_orcids":["https://orcid.org/0000-0001-7029-4233"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Colin D. FUNK","raw_affiliation_strings":["Department of Pharmacology, Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, U.S.A","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028396638","display_name":"Joseph Loscalzo","orcid":"https://orcid.org/0000-0002-1153-8047","observed_orcids":["https://orcid.org/0000-0002-1153-8047"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joseph LOSCALZO","raw_affiliation_strings":["Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, U.S.A","institution_ids":["https://openalex.org/I111088046"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5028396638"],"corresponding_institution_ids":["https://openalex.org/I111088046"],"apc_list":null,"apc_paid":null,"fwci":1.5118,"has_fulltext":true,"cited_by_count":37,"citation_normalized_percentile":{"value":0.83282472,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"351","issue":"3","first_page":"697","last_page":"697"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.4927000105381012,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.4927000105381012,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12910","display_name":"Eicosanoids and Hypertension Pharmacology","score":0.1965000033378601,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.1054999977350235,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/5-lipoxygenase","display_name":"5-lipoxygenase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/nucleotide-binding-site","display_name":"nucleotide binding site","score":0.9829999804496765},{"id":"https://openalex.org/keywords/atp-binding-site","display_name":"ATP binding site","score":0.8539999723434448},{"id":"https://openalex.org/keywords/leukotriene-biosynthesis","display_name":"leukotriene biosynthesis","score":0.8730000257492065},{"id":"https://openalex.org/keywords/arachidonic-acid-metabolism","display_name":"arachidonic acid metabolism","score":0.7839999794960022},{"id":"https://openalex.org/keywords/photoaffinity-labeling","display_name":"photoaffinity labeling","score":0.8999999761581421},{"id":"https://openalex.org/keywords/atp-stimulation","display_name":"ATP stimulation","score":0.578000009059906},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.44699999690055847},{"id":"https://openalex.org/keywords/binding-stoichiometry","display_name":"binding stoichiometry","score":0.5929999947547913},{"id":"https://openalex.org/keywords/tryptic-peptide-mapping","display_name":"tryptic peptide mapping","score":0.4860000014305115},{"id":"https://openalex.org/keywords/enzyme-tertiary-structure","display_name":"enzyme tertiary structure","score":0.4449999928474426}],"concepts":[{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.7811829447746277},{"id":"https://openalex.org/C2780523633","wikidata":"https://www.wikidata.org/wiki/Q380709","display_name":"Labelling","level":2,"score":0.6598342657089233},{"id":"https://openalex.org/C2780679169","wikidata":"https://www.wikidata.org/wiki/Q4688981","display_name":"Affinity label","level":3,"score":0.630915641784668},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.5953482389450073},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5324671268463135},{"id":"https://openalex.org/C2778581200","wikidata":"https://www.wikidata.org/wiki/Q16943198","display_name":"Photoaffinity labeling","level":3,"score":0.5067923665046692},{"id":"https://openalex.org/C118716","wikidata":"https://www.wikidata.org/wiki/Q5514725","display_name":"GTP'","level":3,"score":0.45840007066726685},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4281647503376007},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3900453448295593},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3611970543861389},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.35376498103141785},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000345","descriptor_name":"Affinity Labels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000345","descriptor_name":"Affinity Labels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000345","descriptor_name":"Affinity Labels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1042/0264-6021:3510697","doi":"https://doi.org/10.1042/0264-6021:3510697","is_oa":false,"landing_page_url":"https://doi.org/10.1042/0264-6021:3510697","pdf_url":null,"source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"doi:10.1042/bj3510697","doi":"https://doi.org/10.1042/bj3510697","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj3510697","pdf_url":"https://portlandpress.com/biochemj/article-pdf/351/3/697/736957/bj3510697.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1221410","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1221410","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:11042125","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11042125","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1042/bj3510697","doi":"https://doi.org/10.1042/bj3510697","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj3510697","pdf_url":"https://portlandpress.com/biochemj/article-pdf/351/3/697/736957/bj3510697.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.4099999964237213,"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G106513794","display_name":null,"funder_award_id":"DK 19525","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G1434725642","display_name":"BIOCHEMISTRY/BIOLOGY OF 5-LIPOXYGENASE AND LEUKOTRIENES","funder_award_id":"2r01hl058464-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2006862495","display_name":null,"funder_award_id":"HL53919","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G3671308474","display_name":"S-NITROSOTHIOLS AND EDRF ACTION AND METABOLISM","funder_award_id":"5r01hl053919-03","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G709529962","display_name":null,"funder_award_id":"HL 58464","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G9000878197","display_name":"University of Pennsylvania Diabetes Research Center","funder_award_id":"P30DK019525","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320309370","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10"},{"id":"https://openalex.org/F4320309810","display_name":"Stiftung VERUM","ror":"https://ror.org/00qmq1337"},{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320322315","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320334626","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2039737101.pdf","grobid_xml":"https://content.openalex.org/works/W2039737101.grobid-xml"},"referenced_works_count":49,"referenced_works":["https://openalex.org/W47210259","https://openalex.org/W989164380","https://openalex.org/W1040351076","https://openalex.org/W1506578309","https://openalex.org/W1510143258","https://openalex.org/W1564614506","https://openalex.org/W1567023633","https://openalex.org/W1584305148","https://openalex.org/W1598210890","https://openalex.org/W1605709450","https://openalex.org/W1665697053","https://openalex.org/W1668867309","https://openalex.org/W1966164413","https://openalex.org/W1975207791","https://openalex.org/W1975643364","https://openalex.org/W1982011488","https://openalex.org/W1983617704","https://openalex.org/W1994862373","https://openalex.org/W2009501431","https://openalex.org/W2019266913","https://openalex.org/W2024176341","https://openalex.org/W2024819037","https://openalex.org/W2025419911","https://openalex.org/W2025738476","https://openalex.org/W2033603399","https://openalex.org/W2041823256","https://openalex.org/W2047359965","https://openalex.org/W2050669947","https://openalex.org/W2052888487","https://openalex.org/W2054044627","https://openalex.org/W2068261691","https://openalex.org/W2076677399","https://openalex.org/W2084892030","https://openalex.org/W2086748034","https://openalex.org/W2089073520","https://openalex.org/W2098036595","https://openalex.org/W2125645759","https://openalex.org/W2128635872","https://openalex.org/W2129163505","https://openalex.org/W2131744664","https://openalex.org/W2137139967","https://openalex.org/W2152557257","https://openalex.org/W2160234181","https://openalex.org/W2179898537","https://openalex.org/W2180056482","https://openalex.org/W2201551179","https://openalex.org/W2268031825","https://openalex.org/W2337805108","https://openalex.org/W2419338956"],"related_works":["https://openalex.org/W1997308485","https://openalex.org/W1971245070","https://openalex.org/W4416772590","https://openalex.org/W2149898336","https://openalex.org/W97979726","https://openalex.org/W2408785598","https://openalex.org/W2072993592","https://openalex.org/W2427998189","https://openalex.org/W116544985","https://openalex.org/W2005573197"],"abstract_inverted_index":{"5-Lipoxygenase":[0],"(5LO)":[1],"catalyses":[2],"the":[3,8,43,62,70,84,124,148,171,174,209,221,230,234,240,243,245,257,264],"first":[4],"two":[5,185,246],"steps":[6],"in":[7,38,200,208,215,256],"biosynthesis":[9],"of":[10,50,72,78,83,89,91,103,105,173,187,233,237,242,249,267],"leukotrienes,":[11],"which":[12,94,108,162],"are":[13],"inflammatory":[14],"mediators":[15],"derived":[16],"from":[17],"arachidonic":[18],"acid.":[19],"5LO":[20,51,59,79,92,106,165,188,210,250],"activity":[21],"is":[22],"stimulated":[23],"by":[24,130,191,220],"ATP;":[25],"however,":[26],"a":[27,151,157],"consensus":[28],"ATP-binding":[29,159,265],"site":[30,33,127,149,154,266],"or":[31,100,262],"nucleotide-binding":[32,153,175],"has":[34],"not":[35],"been":[36],"found":[37],"its":[39],"protein":[40],"sequence.":[41,211],"In":[42],"present":[44],"study,":[45],"affinity":[46],"and":[47,55,66,68,139,180,205,213],"photoaffinity":[48],"labelling":[49,85,119,172],"with":[52,97,111,115,120,143,178],"5'-p-fluorosulphonylbenzoyladenosine":[53],"(FSBA)":[54],"2-azido-ATP":[56,144],"showed":[57,183],"that":[58,69,123,147,184,224],"bound":[60],"to":[61,196,229],"ATP":[63,76,95,109],"analogues":[64],"quantitatively":[65],"specifically":[67],"incorporation":[71],"either":[73],"analogue":[74],"inhibited":[75],"stimulation":[77],"activity.":[80],"The":[81],"stoichiometry":[82,241],"was":[86,128,150],"1.4":[87],"mol":[88,102],"FSBA/mol":[90],"(of":[93,107],"competed":[96,110,142],"1":[98],"mol/mol)":[99],"0.94":[101],"2-azido-ATP/mol":[104],"0.77":[112],"mol/mol).":[113],"Labelling":[114],"FSBA":[116],"prevented":[117],"further":[118],"2-azido-ATP,":[121],"indicating":[122],"same":[125],"binding":[126],"occupied":[129],"both":[131],"analogues.":[132],"Other":[133],"nucleotides":[134],"(ADP,":[135],"AMP,":[136],"GTP,":[137],"CTP":[138],"UTP)":[140],"also":[141,163],"labelling,":[145,222,244],"suggesting":[146,223],"general":[152],"rather":[155],"than":[156],"strict":[158],"site.":[160,176],"Ca(2+),":[161],"stimulates":[164],"activity,":[166],"had":[167],"no":[168],"effect":[169],"on":[170],"Digestion":[177],"trypsin":[179],"peptide":[181,247],"sequencing":[182],"fragments":[186,194],"were":[189,218,226,251],"labelled":[190],"2-azido-ATP.":[192],"These":[193],"correspond":[195],"residues":[197],"73-83":[198],"(KYWLNDDWYLK,":[199],"single-letter":[201],"amino":[202],"acid":[203],"code)":[204],"193-209":[206],"(FMHMFQSSWNDFADFEK)":[207],"Trp-75":[212],"Trp-201":[214],"these":[216],"peptides":[217],"modified":[219],"they":[225],"immediately":[227],"adjacent":[228],"C-2":[231],"position":[232],"adenine":[235],"ring":[236],"ATP.":[238],"Given":[239],"sequences":[248],"probably":[252],"near":[253],"each":[254],"other":[255],"enzyme's":[258],"tertiary":[259],"structure,":[260],"composing":[261],"surrounding":[263],"5LO.":[268]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2016-06-24T00:00:00"}