{"id":"https://openalex.org/W2038768445","doi":"https://doi.org/10.7589/0090-3558-22.4.468","title":"IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES","display_name":"IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES","publication_year":1986,"publication_date":"1986-10-01","ids":{"openalex":"https://openalex.org/W2038768445","doi":"https://doi.org/10.7589/0090-3558-22.4.468","mag":"2038768445","pmid":"https://pubmed.ncbi.nlm.nih.gov/3503131"},"language":"en","primary_location":{"id":"doi:10.7589/0090-3558-22.4.468","doi":"https://doi.org/10.7589/0090-3558-22.4.468","is_oa":true,"landing_page_url":"https://doi.org/10.7589/0090-3558-22.4.468","pdf_url":"https://meridian.allenpress.com/jwd/article-pdf/22/4/468/2231596/0090-3558-22_4_468.pdf","source":{"id":"https://openalex.org/S113896437","display_name":"Journal of Wildlife Diseases","issn_l":"0090-3558","issn":["0090-3558","1943-3700"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322697125","host_organization_name":"Wildlife Disease Association","host_organization_lineage":["https://openalex.org/P4322697125"],"host_organization_lineage_names":["Wildlife Disease Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Wildlife Diseases","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://meridian.allenpress.com/jwd/article-pdf/22/4/468/2231596/0090-3558-22_4_468.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109190859","display_name":"Philip E. McAllister","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1343028186","display_name":"United States Fish and Wildlife Service","ror":"https://ror.org/04k7dar27","country_code":"US","type":"government","lineage":["https://openalex.org/I1335927249","https://openalex.org/I1343028186"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. E. McAllister","raw_affiliation_strings":["U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Box 700, Keameysville, West Virginia 25430, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Box 700, Keameysville, West Virginia 25430, USA","institution_ids":["https://openalex.org/I1343028186"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071420472","display_name":"William Bane Schill","orcid":"https://orcid.org/0000-0002-9217-984X","observed_orcids":["https://orcid.org/0000-0002-9217-984X"]},"institutions":[{"id":"https://openalex.org/I1343028186","display_name":"United States Fish and Wildlife Service","ror":"https://ror.org/04k7dar27","country_code":"US","type":"government","lineage":["https://openalex.org/I1335927249","https://openalex.org/I1343028186"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W. B. Schill","raw_affiliation_strings":["U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Box 700, Keameysville, West Virginia 25430, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Box 700, Keameysville, West Virginia 25430, USA","institution_ids":["https://openalex.org/I1343028186"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1343028186"],"apc_list":null,"apc_paid":null,"fwci":0.7178,"has_fulltext":true,"cited_by_count":46,"citation_normalized_percentile":{"value":0.68762111,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"22","issue":"4","first_page":"468","last_page":"474"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/immunoblot-assay","display_name":"immunoblot assay","score":0.9810000061988831},{"id":"https://openalex.org/keywords/salmonid-viruses","display_name":"salmonid viruses","score":0.875},{"id":"https://openalex.org/keywords/infectious-pancreatic-necrosis","display_name":"infectious pancreatic necrosis","score":0.9459999799728394},{"id":"https://openalex.org/keywords/infectious-hematopoietic-necrosis","display_name":"infectious hematopoietic necrosis","score":1.0},{"id":"https://openalex.org/keywords/viral-haemorrhagic-septicaemia","display_name":"viral haemorrhagic septicaemia","score":0.9959999918937683},{"id":"https://openalex.org/keywords/viral-antigen-detection","display_name":"viral antigen detection","score":0.8679999709129333},{"id":"https://openalex.org/keywords/cell-culture-supernatant","display_name":"cell culture supernatant","score":0.8560000061988831},{"id":"https://openalex.org/keywords/nitrocellulose-membrane","display_name":"nitrocellulose membrane","score":0.8130000233650208},{"id":"https://openalex.org/keywords/assay-sensitivity","display_name":"assay sensitivity","score":0.597000002861023},{"id":"https://openalex.org/keywords/fish-disease-diagnosis","display_name":"fish disease diagnosis","score":0.5440000295639038}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8128658533096313},{"id":"https://openalex.org/C2908884048","wikidata":"https://www.wikidata.org/wiki/Q1662348","display_name":"Infectious pancreatic necrosis virus","level":3,"score":0.599671483039856},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5946882367134094},{"id":"https://openalex.org/C2778966499","wikidata":"https://www.wikidata.org/wiki/Q880931","display_name":"Infectious hematopoietic necrosis virus","level":4,"score":0.5817974209785461},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5100560784339905},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.48116692900657654},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.47079554200172424},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.4420194625854492},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.37347161769866943},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.16435882449150085},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.14161264896392822},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C2994167347","wikidata":"https://www.wikidata.org/wiki/Q187986","display_name":"Rainbow trout","level":3,"score":0.0},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005393","descriptor_name":"Fish Diseases","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D005398","descriptor_name":"Fisheries","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012209","descriptor_name":"Rhabdoviridae","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012487","descriptor_name":"Salmonidae","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D014777","descriptor_name":"Virus Diseases","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D014777","descriptor_name":"Virus Diseases","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.7589/0090-3558-22.4.468","doi":"https://doi.org/10.7589/0090-3558-22.4.468","is_oa":true,"landing_page_url":"https://doi.org/10.7589/0090-3558-22.4.468","pdf_url":"https://meridian.allenpress.com/jwd/article-pdf/22/4/468/2231596/0090-3558-22_4_468.pdf","source":{"id":"https://openalex.org/S113896437","display_name":"Journal of Wildlife Diseases","issn_l":"0090-3558","issn":["0090-3558","1943-3700"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322697125","host_organization_name":"Wildlife Disease Association","host_organization_lineage":["https://openalex.org/P4322697125"],"host_organization_lineage_names":["Wildlife Disease Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Wildlife Diseases","raw_type":"journal-article"},{"id":"pmid:3503131","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3503131","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of wildlife diseases","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.7589/0090-3558-22.4.468","doi":"https://doi.org/10.7589/0090-3558-22.4.468","is_oa":true,"landing_page_url":"https://doi.org/10.7589/0090-3558-22.4.468","pdf_url":"https://meridian.allenpress.com/jwd/article-pdf/22/4/468/2231596/0090-3558-22_4_468.pdf","source":{"id":"https://openalex.org/S113896437","display_name":"Journal of Wildlife Diseases","issn_l":"0090-3558","issn":["0090-3558","1943-3700"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322697125","host_organization_name":"Wildlife Disease Association","host_organization_lineage":["https://openalex.org/P4322697125"],"host_organization_lineage_names":["Wildlife Disease Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Wildlife Diseases","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320333526","display_name":"Bonneville Power Administration","ror":"https://ror.org/05mb2ak88"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2038768445.pdf","grobid_xml":"https://content.openalex.org/works/W2038768445.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W129350819","https://openalex.org/W322792558","https://openalex.org/W1584938436","https://openalex.org/W1660780333","https://openalex.org/W1827087473","https://openalex.org/W1967219603","https://openalex.org/W1982472420","https://openalex.org/W1997307220","https://openalex.org/W2016133866","https://openalex.org/W2063180740","https://openalex.org/W2121033894","https://openalex.org/W2121155948","https://openalex.org/W2151776629","https://openalex.org/W2154567769","https://openalex.org/W2167309585","https://openalex.org/W2209273981"],"related_works":["https://openalex.org/W2078221618","https://openalex.org/W2068583415","https://openalex.org/W139647699","https://openalex.org/W1563600938","https://openalex.org/W1660780333","https://openalex.org/W1994130598","https://openalex.org/W2045040440","https://openalex.org/W322792558","https://openalex.org/W1968339963","https://openalex.org/W2154567769"],"abstract_inverted_index":{"An":[0],"immunoblot":[1,45,66,86],"assay":[2,42,46,87],"was":[3,27,68,88],"used":[4,89],"to":[5,52,90],"identify":[6,93],"the":[7],"viruses":[8],"of":[9,82],"infectious":[10,13],"pancreatic":[11],"necrosis,":[12,15],"hematopoietic":[14],"and":[16,37,54,92],"viral":[17,83],"hemorrhagic":[18],"septicemia.":[19],"Viral":[20],"antigen":[21],"in":[22,95],"infected":[23],"cell":[24,61,96],"culture":[25,62,97],"supernatant":[26,63],"adsorbed":[28],"onto":[29],"nitrocellulose":[30],"membrane":[31],"or":[32],"Whatman":[33],"541":[34],"filter":[35],"paper":[36],"detected":[38],"by":[39],"enzyme-linked":[40],"immunosorbent":[41],"techniques.":[43],"The":[44,85],"took":[47],"less":[48],"than":[49],"4":[50],"hr":[51],"perform":[53],"required":[55],"no":[56],"special":[57],"instrumentation.":[58],"Assays":[59],"using":[60,76],"fluids":[64],"showed":[65],"sensitivity":[67],"10(5)":[69],"-":[70],"10(6)":[71],"PFU/ml.":[72],"Assay":[73],"sensitivity,":[74],"determined":[75],"purified":[77],"virus,":[78],"is":[79],"0.85-4.0":[80],"ng":[81],"antigen.":[84],"detect":[91],"virus":[94],"fluids.":[98]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}