{"id":"https://openalex.org/W2038484227","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a022764","title":"Ligand-Binding Properties of Annexin from Caenorhabditis elegans (Annexin XVI, Nex-1)","display_name":"Ligand-Binding Properties of Annexin from Caenorhabditis elegans (Annexin XVI, Nex-1)","publication_year":2000,"publication_date":"2000-09-01","ids":{"openalex":"https://openalex.org/W2038484227","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a022764","mag":"2038484227","pmid":"https://pubmed.ncbi.nlm.nih.gov/10965035"},"language":"en","primary_location":{"id":"doi:10.1093/oxfordjournals.jbchem.a022764","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a022764","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a022764","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024489564","display_name":"Ayano Satoh","orcid":"https://orcid.org/0000-0003-3736-1283","observed_orcids":["https://orcid.org/0000-0003-3736-1283"]},"institutions":[{"id":"https://openalex.org/I26120043","display_name":"Ochanomizu University","ror":"https://ror.org/03599d813","country_code":"JP","type":"education","lineage":["https://openalex.org/I26120043"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"A. Satoh","raw_affiliation_strings":["The Graduate School of Humanities and Sciences, Department of Chemistry, Faculty of Science, Ochanomizu University, Otsuka Bunkyo-ku, Tokyo 112-8610, Japan. isamu@cc.ocha.ac.jp","The Graduate School of Humanities and SciencesOtsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Graduate School of Humanities and Sciences, Department of Chemistry, Faculty of Science, Ochanomizu University, Otsuka Bunkyo-ku, Tokyo 112-8610, Japan. isamu@cc.ocha.ac.jp","institution_ids":["https://openalex.org/I26120043"]},{"raw_affiliation_string":"The Graduate School of Humanities and SciencesOtsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5096864525","display_name":"M. Hazuki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I26120043","display_name":"Ochanomizu University","ror":"https://ror.org/03599d813","country_code":"JP","type":"education","lineage":["https://openalex.org/I26120043"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"M. Hazuki","raw_affiliation_strings":["\u2020 Department of Chemistry Faculty of Science Ochanomizu University Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u2020 Department of Chemistry Faculty of Science Ochanomizu University Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610","institution_ids":["https://openalex.org/I26120043"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040490999","display_name":"Keisuke Kojima","orcid":"https://orcid.org/0000-0001-8430-384X","observed_orcids":["https://orcid.org/0000-0001-8430-384X"]},"institutions":[{"id":"https://openalex.org/I26120043","display_name":"Ochanomizu University","ror":"https://ror.org/03599d813","country_code":"JP","type":"education","lineage":["https://openalex.org/I26120043"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K. Kojima","raw_affiliation_strings":["\u2020 Department of Chemistry Faculty of Science Ochanomizu University Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u2020 Department of Chemistry Faculty of Science Ochanomizu University Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610","institution_ids":["https://openalex.org/I26120043"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002952594","display_name":"Jun Hirabayashi","orcid":"https://orcid.org/0000-0002-8571-359X","observed_orcids":["https://orcid.org/0000-0002-8571-359X"]},"institutions":[{"id":"https://openalex.org/I87531838","display_name":"Teikyo University","ror":"https://ror.org/01gaw2478","country_code":"JP","type":"education","lineage":["https://openalex.org/I87531838"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"J. Hirabayashi","raw_affiliation_strings":["\u2021 Departement of Biological Chemistry Faculty of Pharamaceutical Sciences Teikyo University Sagamiko, Kanagawa 199-01"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u2021 Departement of Biological Chemistry Faculty of Pharamaceutical Sciences Teikyo University Sagamiko, Kanagawa 199-01","institution_ids":["https://openalex.org/I87531838"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089219803","display_name":"I. Matsumoto","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I. Matsumoto","raw_affiliation_strings":["The Graduate School of Humanities and SciencesOtsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Graduate School of Humanities and SciencesOtsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3276,"currency":"USD","value_usd":3276},"apc_paid":null,"fwci":0.6017,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":{"value":0.64253737,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"128","issue":"3","first_page":"377","last_page":"381"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12030","display_name":"S100 Proteins and Annexins","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12030","display_name":"S100 Proteins and Annexins","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/annexins","display_name":"annexins","score":0.7009999752044678},{"id":"https://openalex.org/keywords/caenorhabditis-elegans","display_name":"Caenorhabditis elegans","score":0.9539999961853027},{"id":"https://openalex.org/keywords/ligand-binding","display_name":"ligand binding","score":0.8299999833106995},{"id":"https://openalex.org/keywords/phospholipid-binding","display_name":"phospholipid binding","score":0.8880000114440918},{"id":"https://openalex.org/keywords/phosphatidylserine","display_name":"phosphatidylserine","score":0.9390000104904175},{"id":"https://openalex.org/keywords/glycosaminoglycan-binding","display_name":"glycosaminoglycan binding","score":0.9359999895095825},{"id":"https://openalex.org/keywords/heparan-sulfate","display_name":"heparan sulfate","score":0.9150000214576721},{"id":"https://openalex.org/keywords/chondroitin-sulfate","display_name":"chondroitin sulfate","score":0.921999990940094},{"id":"https://openalex.org/keywords/heparin","display_name":"heparin","score":0.9150000214576721},{"id":"https://openalex.org/keywords/perlecan","display_name":"perlecan","score":0.8899999856948853},{"id":"https://openalex.org/keywords/calcium-dependent-binding","display_name":"calcium-dependent binding","score":0.8650000095367432},{"id":"https://openalex.org/keywords/binding-affinity","display_name":"binding affinity","score":0.6570000052452087}],"concepts":[{"id":"https://openalex.org/C2778041096","wikidata":"https://www.wikidata.org/wiki/Q424668","display_name":"Heparan sulfate","level":3,"score":0.7440592646598816},{"id":"https://openalex.org/C2779553658","wikidata":"https://www.wikidata.org/wiki/Q408014","display_name":"Chondroitin sulfate","level":3,"score":0.6314575672149658},{"id":"https://openalex.org/C2779637612","wikidata":"https://www.wikidata.org/wiki/Q2354337","display_name":"Phosphatidylserine","level":4,"score":0.6290409564971924},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6070595383644104},{"id":"https://openalex.org/C2776735749","wikidata":"https://www.wikidata.org/wiki/Q4862650","display_name":"Chondroitin","level":3,"score":0.6056370735168457},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5686995983123779},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.5488768219947815},{"id":"https://openalex.org/C30345302","wikidata":"https://www.wikidata.org/wiki/Q14908233","display_name":"Annexin A2","level":4,"score":0.5266342759132385},{"id":"https://openalex.org/C88634738","wikidata":"https://www.wikidata.org/wiki/Q24769543","display_name":"Annexin","level":3,"score":0.5125257968902588},{"id":"https://openalex.org/C153074725","wikidata":"https://www.wikidata.org/wiki/Q407553","display_name":"Glycosaminoglycan","level":2,"score":0.4938986897468567},{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.4902117848396301},{"id":"https://openalex.org/C185154212","wikidata":"https://www.wikidata.org/wiki/Q410454","display_name":"Liposome","level":2,"score":0.4796867072582245},{"id":"https://openalex.org/C2778944004","wikidata":"https://www.wikidata.org/wiki/Q91703","display_name":"Caenorhabditis elegans","level":3,"score":0.4608127474784851},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.43670791387557983},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.4131513237953186},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3174440264701843},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.12894892692565918},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.1076040267944336},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001667","descriptor_name":"Binding, Competitive","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002809","descriptor_name":"Chondroitin Sulfates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006025","descriptor_name":"Glycosaminoglycans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006493","descriptor_name":"Heparin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006497","descriptor_name":"Heparan Sulfate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008081","descriptor_name":"Liposomes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010718","descriptor_name":"Phosphatidylserines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017173","descriptor_name":"Caenorhabditis elegans","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D017302","descriptor_name":"Annexins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017302","descriptor_name":"Annexins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D017302","descriptor_name":"Annexins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029742","descriptor_name":"Caenorhabditis elegans Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1093/oxfordjournals.jbchem.a022764","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a022764","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a022764","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:10965035","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10965035","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2084469870","https://openalex.org/W4388386300","https://openalex.org/W2085923483","https://openalex.org/W2059175007","https://openalex.org/W2017043638","https://openalex.org/W4211171489","https://openalex.org/W2090044857","https://openalex.org/W2072883399","https://openalex.org/W1977158442","https://openalex.org/W7144966896"],"abstract_inverted_index":{"Annexins":[0,28],"are":[1,29],"structurally":[2],"related":[3],"proteins":[4],"that":[5,15],"bind":[6,22],"phospholipids":[7],"in":[8,24],"a":[9,25],"calcium-dependent":[10,26],"manner.":[11,27],"Recently,":[12],"we":[13,52],"showed":[14],"annexins":[16],"IV,":[17],"V,":[18],"and":[19,37,108,117,124],"VI":[20],"also":[21],"glycosaminoglycans":[23],"widely":[30],"distributed":[31],"from":[32],"lower":[33],"to":[34,45,64,75,80,113,122],"higher":[35],"eukaryotes,":[36],"the":[38,54,83,90],"nematode":[39],"Caenorhabditis":[40],"elegans":[41],"has":[42],"been":[43],"found":[44],"contain":[46],"Nex-1,":[47],"an":[48],"annexin":[49],"homologue.":[50],"Here,":[51],"characterize":[53],"ligand-binding":[55],"properties":[56],"of":[57,96,144],"Nex-1":[58,62,84,100,111],"using":[59],"recombinant":[60],"Nex-1.":[61,145],"binds":[63,112],"liposomes":[65],"containing":[66],"phosphatidylserine.":[67],"The":[68,99],"apparent":[69],"K(d)":[70,91],"was":[71],"calculated":[72],"by":[73,105],"Biacore":[74],"be":[76,141],"4.4":[77],"nM.":[78],"Compared":[79],"mammalian":[81],"annexins,":[82],"phospholipid-binding":[85],"specificities":[86,102],"were":[87,93,103],"similar":[88],"whereas":[89],"values":[92],"one":[94],"order":[95],"magnitude":[97],"larger.":[98],"glycosaminoglycan-binding":[101],"investigated":[104],"affinity":[106],"chromatography":[107],"solid-phase":[109],"assays.":[110],"heparin,":[114],"heparan":[115,132],"sulfate,":[116],"chondroitin":[118,123,135],"sulfate":[119,133],"but":[120],"not":[121],"chemically":[125],"N-":[126],"or":[127],"O-desulfated":[128],"heparin.":[129],"Besides":[130],"phospholipids,":[131],"and/or":[134],"(sulfate),":[136],"probably":[137],"on":[138],"perlecan,":[139],"could":[140],"endogenous":[142],"ligands":[143]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}