{"id":"https://openalex.org/W2037780745","doi":"https://doi.org/10.1073/pnas.1118018109","title":"Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes","display_name":"Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes","publication_year":2012,"publication_date":"2012-01-09","ids":{"openalex":"https://openalex.org/W2037780745","doi":"https://doi.org/10.1073/pnas.1118018109","mag":"2037780745","pmid":"https://pubmed.ncbi.nlm.nih.gov/22232676","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3268301"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.1118018109","doi":"https://doi.org/10.1073/pnas.1118018109","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.1118018109","pdf_url":"https://www.pnas.org/content/pnas/109/4/1347.full.pdf","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.pnas.org/content/pnas/109/4/1347.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009414246","display_name":"Katsuyuki Shiroguchi","orcid":"https://orcid.org/0000-0003-4025-8789","observed_orcids":["https://orcid.org/0000-0003-4025-8789"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katsuyuki Shiroguchi","raw_affiliation_strings":["Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138,, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138,, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021192553","display_name":"Tony Z. Jia","orcid":"https://orcid.org/0000-0001-5175-4599","observed_orcids":["https://orcid.org/0000-0001-5175-4599"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tony Z. Jia","raw_affiliation_strings":["Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","Department of Chemistry and Chemical Biology, Harvard University 12 Oxford St., Cambridge, Massachusetts 02138"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University 12 Oxford St., Cambridge, Massachusetts 02138","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066080208","display_name":"Peter A. Sims","orcid":"https://orcid.org/0000-0002-3921-4837","observed_orcids":["https://orcid.org/0000-0002-3921-4837"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter A. Sims","raw_affiliation_strings":["Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","Department of Chemistry and Chemical Biology, Harvard University 12 Oxford St., Cambridge, Massachusetts 02138"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University 12 Oxford St., Cambridge, Massachusetts 02138","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102919090","display_name":"Xiaoliang Sunney Xie","orcid":"https://orcid.org/0000-0001-7714-9776","observed_orcids":["https://orcid.org/0000-0001-7714-9776"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"X. Sunney Xie","raw_affiliation_strings":["Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I136199984"],"apc_list":null,"apc_paid":null,"fwci":8.3496,"has_fulltext":true,"cited_by_count":275,"citation_normalized_percentile":{"value":0.98750795,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"109","issue":"4","first_page":"1347","last_page":"1352"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.7943999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.7943999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.18569999933242798,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gene-expression-profiling","display_name":"gene expression profiling","score":0.6579999923706055}],"concepts":[{"id":"https://openalex.org/C2776841711","wikidata":"https://www.wikidata.org/wiki/Q856","display_name":"Barcode","level":2,"score":0.7780678868293762},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6373610496520996},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6136784553527832},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5711750388145447},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.5701625943183899},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.4917357265949249},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4520745277404785},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.44061052799224854},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.4266617000102997},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.42430901527404785},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.4230448007583618},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2682207226753235},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.26214560866355896},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.26190775632858276},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.17532381415367126},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.13070735335350037},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D049490","descriptor_name":"Systems Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D058893","descriptor_name":"DNA Barcoding, Taxonomic","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1073/pnas.1118018109","doi":"https://doi.org/10.1073/pnas.1118018109","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.1118018109","pdf_url":"https://www.pnas.org/content/pnas/109/4/1347.full.pdf","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2405487","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3268301","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3268301","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3268301","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:22232676","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22232676","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:dash.harvard.edu:1/10919794","doi":null,"is_oa":true,"landing_page_url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:10919794","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"},{"id":"pmh:oai:irdb.nii.ac.jp:00897:0004893826","doi":null,"is_oa":false,"landing_page_url":"http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100847264","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal article"},{"id":"pmh:oai:t2r2.star.titech.ac.jp:50571896","doi":null,"is_oa":false,"landing_page_url":"http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100847264","pdf_url":null,"source":{"id":"https://openalex.org/S4377196385","display_name":"Tokyo Tech Research Repository (Tokyo Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114531698","host_organization_name":"Tokyo Institute of Technology","host_organization_lineage":["https://openalex.org/I114531698"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1073/pnas.1118018109","doi":"https://doi.org/10.1073/pnas.1118018109","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.1118018109","pdf_url":"https://www.pnas.org/content/pnas/109/4/1347.full.pdf","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3497447825","display_name":null,"funder_award_id":"HG005097-01","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G4818838254","display_name":"Single Cell Single Molecule Digital mRNA Profiling with No PCR Amplification","funder_award_id":"RC2HG005613","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G5854869349","display_name":"Real-time single-molecule nucleic acid sequencing with fluorogenic nucleotides","funder_award_id":"R01HG005097","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320310760","display_name":"Uehara Memorial Foundation","ror":"https://ror.org/00gc20a07"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332591","display_name":"Intellectual and Developmental Disabilities Research Center","ror":"https://ror.org/02pttbw34"},{"id":"https://openalex.org/F4320332618","display_name":"Faculty of Arts and Sciences","ror":"https://ror.org/03vek6s52"},{"id":"https://openalex.org/F4320332648","display_name":"Tufts University School of Medicine","ror":"https://ror.org/05wvpxv85"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2037780745.pdf","grobid_xml":"https://content.openalex.org/works/W2037780745.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W1965175512","https://openalex.org/W1969435169","https://openalex.org/W1979072451","https://openalex.org/W1981509058","https://openalex.org/W1984708728","https://openalex.org/W1984857185","https://openalex.org/W1995830102","https://openalex.org/W2007844697","https://openalex.org/W2012016911","https://openalex.org/W2019766856","https://openalex.org/W2025836692","https://openalex.org/W2028319246","https://openalex.org/W2031212650","https://openalex.org/W2040712240","https://openalex.org/W2041677964","https://openalex.org/W2053184946","https://openalex.org/W2066801022","https://openalex.org/W2079517684","https://openalex.org/W2092387193","https://openalex.org/W2099320873","https://openalex.org/W2104806799","https://openalex.org/W2119278037","https://openalex.org/W2122789575","https://openalex.org/W2129795133","https://openalex.org/W2131088968","https://openalex.org/W2133385595","https://openalex.org/W2139915240","https://openalex.org/W2142565931","https://openalex.org/W2149573463","https://openalex.org/W2152241467","https://openalex.org/W2154431984","https://openalex.org/W2160444236","https://openalex.org/W2167557255","https://openalex.org/W2579829632"],"related_works":["https://openalex.org/W7099326920","https://openalex.org/W4391539944","https://openalex.org/W2055181847","https://openalex.org/W1977081991","https://openalex.org/W2162554386","https://openalex.org/W2763205649","https://openalex.org/W2183282752","https://openalex.org/W2759422348","https://openalex.org/W7161568220","https://openalex.org/W2107602656"],"abstract_inverted_index":{"RNA":[0,92],"sequencing":[1,76,124],"(RNA-Seq)":[2],"is":[3,11,49,58,94,140],"a":[4,29,43,106,149],"powerful":[5],"tool":[6],"for":[7,32,105],"transcriptome":[8,154],"profiling,":[9],"but":[10,145],"hampered":[12],"by":[13,61],"sequence-dependent":[14,134],"bias":[15,135],"and":[16,53,82,136,139,162],"inaccuracy":[17],"at":[18],"low":[19],"copy":[20],"numbers":[21],"intrinsic":[22],"to":[23,67,77,114,142,147],"exponential":[24],"PCR":[25,144],"amplification.":[26],"We":[27,110,152],"developed":[28],"simple":[30],"strategy":[31],"mitigating":[33],"these":[34],"complications,":[35],"allowing":[36],"truly":[37],"digital":[38,143],"RNA-Seq.":[39,167],"Following":[40],"reverse":[41],"transcription,":[42],"large":[44],"set":[45],"of":[46,64,90,100,122,156],"barcode":[47,65,102],"sequences":[48,66,103],"added":[50],"in":[51,119],"excess,":[52],"nearly":[54],"every":[55],"cDNA":[56,83,108],"molecule":[57],"uniquely":[59],"labeled":[60],"random":[62],"attachment":[63],"both":[68],"ends.":[69],"After":[70],"PCR,":[71],"we":[72],"applied":[73],"paired-end":[74],"deep":[75],"read":[78],"the":[79,88,98,112,120],"two":[80],"barcodes":[81,113],"sequences.":[84],"Rather":[85],"than":[86,165],"counting":[87,129],"number":[89,99],"reads,":[91],"abundance":[93],"measured":[95],"based":[96],"on":[97],"unique":[101],"observed":[104],"given":[107],"sequence.":[109],"optimized":[111],"be":[115],"unambiguously":[116],"identifiable,":[117],"even":[118],"presence":[121],"multiple":[123],"errors.":[125],"This":[126],"method":[127],"allows":[128],"with":[130,159],"single-copy":[131],"resolution":[132],"despite":[133],"PCR-amplification":[137],"noise,":[138],"analogous":[141],"amendable":[146],"quantifying":[148],"whole":[150],"transcriptome.":[151],"demonstrated":[153],"profiling":[155],"Escherichia":[157],"coli":[158],"more":[160],"accurate":[161],"reproducible":[163],"quantification":[164],"conventional":[166]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":10},{"year":2024,"cited_by_count":12},{"year":2023,"cited_by_count":14},{"year":2022,"cited_by_count":22},{"year":2021,"cited_by_count":19},{"year":2020,"cited_by_count":16},{"year":2019,"cited_by_count":20},{"year":2018,"cited_by_count":23},{"year":2017,"cited_by_count":26},{"year":2016,"cited_by_count":26},{"year":2015,"cited_by_count":22},{"year":2014,"cited_by_count":31},{"year":2013,"cited_by_count":15},{"year":2012,"cited_by_count":10}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}