{"id":"https://openalex.org/W2037760587","doi":"https://doi.org/10.1271/bbb.60.640","title":"Purification and Properties of a Novel Enzyme, Trehalose Synthase, fromPimelobactersp. R48","display_name":"Purification and Properties of a Novel Enzyme, Trehalose Synthase, fromPimelobactersp. R48","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W2037760587","doi":"https://doi.org/10.1271/bbb.60.640","mag":"2037760587","pmid":"https://pubmed.ncbi.nlm.nih.gov/8829531"},"language":"en","primary_location":{"id":"doi:10.1271/bbb.60.640","doi":"https://doi.org/10.1271/bbb.60.640","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.60.640","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101856337","display_name":"Tomoyuki Nishimoto","orcid":"https://orcid.org/0000-0002-4898-8949","observed_orcids":["https://orcid.org/0000-0002-4898-8949"]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Tomoyuki Nishimoto","raw_affiliation_strings":["Hayashibara Biochemical Laboratories, Inc., Okayama, Japan","Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayashibara Biochemical Laboratories, Inc., Okayama, Japan","institution_ids":["https://openalex.org/I4210104993"]},{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022613202","display_name":"Masayuki Nakano","orcid":"https://orcid.org/0000-0001-7817-7592","observed_orcids":["https://orcid.org/0000-0001-7817-7592"]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masayuki Nakano","raw_affiliation_strings":["Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108451317","display_name":"Tetsuya Nakada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tetsuya Nakada","raw_affiliation_strings":["Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042610568","display_name":"Hiroto Chaen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroto Chaen","raw_affiliation_strings":["Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113610048","display_name":"Shigeharu Fukuda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shigeharu Fukuda","raw_affiliation_strings":["Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Toshiyuki Sugimoto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toshiyuki Sugimoto","raw_affiliation_strings":["Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103372479","display_name":"Masashi Kurimoto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masashi Kurimoto","raw_affiliation_strings":["Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069212057","display_name":"Yoshio Tsujisaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104993","display_name":"Hayashibara (Japan)","ror":"https://ror.org/01rvpy991","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210104993"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshio Tsujisaka","raw_affiliation_strings":["Hayashibara Institute Corp.,, 1\u20132\u20133 Shimoishii, Okayama 700, Japan","Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hayashibara Institute Corp.,, 1\u20132\u20133 Shimoishii, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]},{"raw_affiliation_string":"Hayushibara Biochemical Laboratories, Inc., 7\u20137 Amase-minamimachi, Okayama 700, Japan","institution_ids":["https://openalex.org/I4210104993"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5101856337"],"corresponding_institution_ids":["https://openalex.org/I4210104993"],"apc_list":{"value":3272,"currency":"USD","value_usd":3272},"apc_paid":null,"fwci":2.4452,"has_fulltext":true,"cited_by_count":118,"citation_normalized_percentile":{"value":0.85265439,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"60","issue":"4","first_page":"640","last_page":"644"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8330000042915344,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8330000042915344,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.1574999988079071,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trehalose-synthase","display_name":"trehalose synthase","score":1.0},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8650000095367432},{"id":"https://openalex.org/keywords/trehalose-biosynthesis","display_name":"trehalose biosynthesis","score":0.8220000267028809},{"id":"https://openalex.org/keywords/maltose","display_name":"maltose","score":0.7099999785423279},{"id":"https://openalex.org/keywords/enzyme-properties","display_name":"enzyme properties","score":0.47099998593330383},{"id":"https://openalex.org/keywords/metal-ion-inhibition","display_name":"metal ion inhibition","score":0.6949999928474426},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.4869999885559082},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.39399999380111694}],"concepts":[{"id":"https://openalex.org/C2778197599","wikidata":"https://www.wikidata.org/wiki/Q170002","display_name":"Maltose","level":3,"score":0.9403150081634521},{"id":"https://openalex.org/C2778452849","wikidata":"https://www.wikidata.org/wiki/Q421773","display_name":"Trehalose","level":2,"score":0.9315390586853027},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6985936164855957},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6678926944732666},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5051990151405334},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.49453040957450867},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.47712206840515137},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.45969170331954956},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.44973695278167725},{"id":"https://openalex.org/C2781014771","wikidata":"https://www.wikidata.org/wiki/Q413961","display_name":"Tris","level":2,"score":0.425035297870636}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006093","descriptor_name":"Gram-Positive Asporogenous Rods","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1271/bbb.60.640","doi":"https://doi.org/10.1271/bbb.60.640","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.60.640","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},{"id":"pmid:8829531","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8829531","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, biotechnology, and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W6822430352"],"related_works":["https://openalex.org/W1985579908","https://openalex.org/W2156636217","https://openalex.org/W2366442929","https://openalex.org/W2382737293","https://openalex.org/W2079663619","https://openalex.org/W1978440870","https://openalex.org/W2084833472","https://openalex.org/W2082042773","https://openalex.org/W185498252","https://openalex.org/W2066564362"],"abstract_inverted_index":{"A":[0],"novel":[1],"enzyme,":[2],"trehalose":[3,67,130,141],"synthase,":[4],"was":[5,39,78,90,106,131,159],"purified":[6],"from":[7,142],"a":[8,52],"cell-free":[9],"extract":[10],"of":[11,36,54,64,87,111,125,127,133,140],"Pimelobacter":[12],"sp.":[13],"R48":[14],"to":[15,41,116],"an":[16],"electrophoretically":[17],"homogeneous":[18],"state":[19],"by":[20,44,56,68,161],"successive":[21],"chromatographies":[22],"on":[23,80],"DEAE-Toyopearl":[24],"650,":[25,27],"Butyl-Toyopearl":[26],"and":[28,48,95,99,114,147,152,166],"Mono":[29],"Q":[30],"HR5/5":[31],"columns.":[32],"The":[33,59,71,83,92,104,123,137,157],"molecular":[34],"weight":[35],"the":[37,49,62,88,109,134],"enzyme":[38,50,60,72,89,105],"estimated":[40],"be":[42],"62,000":[43],"SDS-polyacrylamide":[45],"gel":[46,57],"electrophoresis,":[47],"had":[51],"pI":[53],"4.6":[55],"isoelectrofocusing.":[58],"catalyzed":[61],"conversion":[63,126],"maltose":[65,76,128,135,143],"into":[66,75,129],"intramolecular":[69],"transglucosylation.":[70],"also":[73],"converted":[74],"but":[77],"inactive":[79],"other":[81],"saccharides.":[82],"N-terminal":[84],"amino":[85],"acid":[86],"serine.":[91],"optimum":[93],"pH":[94,112],"temperature":[96],"were":[97,144],"pH7.5":[98],"20":[100],"degrees":[101,118,154],"C,":[102,155],"respectively.":[103,156],"stable":[107],"in":[108],"range":[110],"6.0-9.0":[113],"up":[115],"30":[117],"C":[119],"for":[120],"60":[121],"min.":[122],"rate":[124],"independent":[132],"concentration.":[136],"maximum":[138],"yield":[139],"81.8%,":[145],"80.9%,":[146],"76.7%":[148],"at":[149],"5,":[150],"15,":[151],"25":[153],"activity":[158],"inhibited":[160],"Cu2+,":[162],"Hg2+,":[163],"Ni2+,":[164],"Zn2+,":[165],"Tris.":[167]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":10}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
