{"id":"https://openalex.org/W2037320477","doi":"https://doi.org/10.1002/hep.1840070411","title":"The synthesis of proteoglycans in fat-storing cells of rat liver","display_name":"The synthesis of proteoglycans in fat-storing cells of rat liver","publication_year":1987,"publication_date":"1987-07-01","ids":{"openalex":"https://openalex.org/W2037320477","doi":"https://doi.org/10.1002/hep.1840070411","mag":"2037320477","pmid":"https://pubmed.ncbi.nlm.nih.gov/3111965"},"language":"en","primary_location":{"id":"doi:10.1002/hep.1840070411","doi":"https://doi.org/10.1002/hep.1840070411","is_oa":true,"landing_page_url":"https://doi.org/10.1002/hep.1840070411","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/hep.1840070411","source":{"id":"https://openalex.org/S156174673","display_name":"Hepatology","issn_l":"0270-9139","issn":["0270-9139","1527-3350"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hepatology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/hep.1840070411","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076615522","display_name":"Stephan Sch\u00e4fer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stephan Sch\u00e4fer","raw_affiliation_strings":["Department of Clinical Chemistry and Central Laboratory, Philipps-University, D-3550 Marburg, West Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Chemistry and Central Laboratory, Philipps-University, D-3550 Marburg, West Germany","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046991540","display_name":"Ottwin Zerbe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ottwin Zerbe","raw_affiliation_strings":["Department of Clinical Chemistry and Central Laboratory, Philipps-University, D-3550 Marburg, West Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Chemistry and Central Laboratory, Philipps-University, D-3550 Marburg, West Germany","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110680814","display_name":"Axel M. Gressner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Axel M. Gressner","raw_affiliation_strings":["Department of Clinical Chemistry and Central Laboratory, Philipps-University, D-3550 Marburg, West Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Chemistry and Central Laboratory, Philipps-University, D-3550 Marburg, West Germany","institution_ids":["https://openalex.org/I161103922"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5110680814"],"corresponding_institution_ids":["https://openalex.org/I161103922"],"apc_list":{"value":2900,"currency":"USD","value_usd":2900},"apc_paid":null,"fwci":3.5881,"has_fulltext":true,"cited_by_count":255,"citation_normalized_percentile":{"value":0.94502991,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"7","issue":"4","first_page":"680","last_page":"687"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13768","display_name":"Oral and gingival health research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/proteoglycan-synthesis","display_name":"proteoglycan synthesis","score":0.9089999794960022},{"id":"https://openalex.org/keywords/fat-storing-cells","display_name":"fat-storing cells","score":0.9369999766349792},{"id":"https://openalex.org/keywords/glycosaminoglycans","display_name":"glycosaminoglycans","score":0.9819999933242798},{"id":"https://openalex.org/keywords/dermatan-sulfate","display_name":"dermatan sulfate","score":0.9869999885559082},{"id":"https://openalex.org/keywords/chondroitin-sulfate","display_name":"chondroitin sulfate","score":1.0},{"id":"https://openalex.org/keywords/heparan-sulfate","display_name":"heparan sulfate","score":0.996999979019165},{"id":"https://openalex.org/keywords/cell-culture","display_name":"cell culture","score":0.39100000262260437},{"id":"https://openalex.org/keywords/liver-fibrosis","display_name":"liver fibrosis","score":0.7360000014305115},{"id":"https://openalex.org/keywords/liver-cirrhosis","display_name":"liver cirrhosis","score":0.9290000200271606},{"id":"https://openalex.org/keywords/puromycin-inhibition","display_name":"puromycin inhibition","score":0.5699999928474426}],"concepts":[{"id":"https://openalex.org/C2778066728","wikidata":"https://www.wikidata.org/wiki/Q2335618","display_name":"Dermatan sulfate","level":4,"score":0.8976323008537292},{"id":"https://openalex.org/C153074725","wikidata":"https://www.wikidata.org/wiki/Q407553","display_name":"Glycosaminoglycan","level":2,"score":0.7437148094177246},{"id":"https://openalex.org/C2779553658","wikidata":"https://www.wikidata.org/wiki/Q408014","display_name":"Chondroitin sulfate","level":3,"score":0.6601133346557617},{"id":"https://openalex.org/C2778041096","wikidata":"https://www.wikidata.org/wiki/Q424668","display_name":"Heparan sulfate","level":3,"score":0.613038182258606},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6104949712753296},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5612831115722656},{"id":"https://openalex.org/C22731427","wikidata":"https://www.wikidata.org/wiki/Q2071346","display_name":"Perlecan","level":4,"score":0.4758579134941101},{"id":"https://openalex.org/C155138218","wikidata":"https://www.wikidata.org/wiki/Q994559","display_name":"Sulfation","level":2,"score":0.46751028299331665},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4451707601547241},{"id":"https://openalex.org/C518705261","wikidata":"https://www.wikidata.org/wiki/Q25615","display_name":"Connective tissue","level":2,"score":0.43919670581817627},{"id":"https://openalex.org/C2776735749","wikidata":"https://www.wikidata.org/wiki/Q4862650","display_name":"Chondroitin","level":3,"score":0.42558354139328003},{"id":"https://openalex.org/C176891718","wikidata":"https://www.wikidata.org/wiki/Q1997459","display_name":"Hepatic stellate cell","level":2,"score":0.4182778000831604},{"id":"https://openalex.org/C2779335624","wikidata":"https://www.wikidata.org/wiki/Q407976","display_name":"Proteoglycan","level":3,"score":0.4105128049850464},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.27447783946990967},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.23647251725196838},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.15997359156608582},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002809","descriptor_name":"Chondroitin Sulfates","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D003871","descriptor_name":"Dermatan Sulfate","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006025","descriptor_name":"Glycosaminoglycans","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D006497","descriptor_name":"Heparan Sulfate","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011691","descriptor_name":"Puromycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011919","descriptor_name":"Rats, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D050356","descriptor_name":"Lipid Metabolism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/hep.1840070411","doi":"https://doi.org/10.1002/hep.1840070411","is_oa":true,"landing_page_url":"https://doi.org/10.1002/hep.1840070411","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/hep.1840070411","source":{"id":"https://openalex.org/S156174673","display_name":"Hepatology","issn_l":"0270-9139","issn":["0270-9139","1527-3350"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hepatology","raw_type":"journal-article"},{"id":"pmid:3111965","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3111965","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hepatology (Baltimore, Md.)","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/hep.1840070411","doi":"https://doi.org/10.1002/hep.1840070411","is_oa":true,"landing_page_url":"https://doi.org/10.1002/hep.1840070411","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/hep.1840070411","source":{"id":"https://openalex.org/S156174673","display_name":"Hepatology","issn_l":"0270-9139","issn":["0270-9139","1527-3350"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hepatology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310905","display_name":"Philipps-Universit\u00e4t Marburg","ror":"https://ror.org/01rdrb571"},{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2037320477.pdf","grobid_xml":"https://content.openalex.org/works/W2037320477.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W125827992","https://openalex.org/W140193100","https://openalex.org/W318069809","https://openalex.org/W563514949","https://openalex.org/W1553693482","https://openalex.org/W1647844745","https://openalex.org/W1775749144","https://openalex.org/W1783978306","https://openalex.org/W1927702051","https://openalex.org/W1965427178","https://openalex.org/W1977779461","https://openalex.org/W1985554541","https://openalex.org/W1999078180","https://openalex.org/W2002664674","https://openalex.org/W2002923080","https://openalex.org/W2013159469","https://openalex.org/W2021964163","https://openalex.org/W2035781715","https://openalex.org/W2046827702","https://openalex.org/W2047567629","https://openalex.org/W2048901294","https://openalex.org/W2050569765","https://openalex.org/W2054197186","https://openalex.org/W2066681349","https://openalex.org/W2085548901","https://openalex.org/W2088071075","https://openalex.org/W2115379962","https://openalex.org/W2121411086","https://openalex.org/W2144274337","https://openalex.org/W2154663821","https://openalex.org/W2164728409","https://openalex.org/W2278012352","https://openalex.org/W2330819842","https://openalex.org/W2409453570","https://openalex.org/W2418348679","https://openalex.org/W2443702444","https://openalex.org/W2465993798","https://openalex.org/W2469094094","https://openalex.org/W2574643900","https://openalex.org/W2766633068","https://openalex.org/W3128972822","https://openalex.org/W4300113397"],"related_works":["https://openalex.org/W2320355698","https://openalex.org/W2144818517","https://openalex.org/W7100723367","https://openalex.org/W1854827911","https://openalex.org/W2046827702","https://openalex.org/W2006862893","https://openalex.org/W2057779031","https://openalex.org/W36543017","https://openalex.org/W1669912892","https://openalex.org/W2416560762"],"abstract_inverted_index":{"Fat-storing":[0],"cells":[1,25,42,89,191,214],"(perisinusoidal":[2],"stellate":[3],"cells)":[4],"were":[5,73],"isolated":[6],"by":[7,15,162,174],"enzymatic":[8],"digestion":[9,164],"of":[10,31,36,40,52,69,79,82,123,133,154,158,171,181,197,210,212,229,235],"rat":[11],"liver":[12,204,222],"and":[13,33,46,62,90,114,165,167,200,238],"purified":[14],"a":[16,29,34,85,107,193],"single-step":[17],"Nycodenz":[18],"gradient":[19],"to":[20,96],"yield":[21],"11.4":[22],"X":[23],"10(6)":[24],"per":[26],"liver,":[27],"with":[28,55],"purity":[30],"74%":[32],"viability":[35],"76%.":[37],"Monolayer":[38],"cultures":[39,139],"fat-storing":[41,190,213],"incorporated":[43],"both":[44],"[35S]sulfate":[45],"[3H]glucosamine":[47],"into":[48,75],"glycosaminoglycans;":[49],"the":[50,60,76,80,120,126,145,149,155,168,179,208,220,233],"rate":[51],"incorporation":[53],"increased":[54],"culture":[56,97],"time":[57],"(3-fold":[58],"between":[59],"third":[61],"eighth":[63],"days":[64],"in":[65,125,148,203,219,241],"culture).":[66],"About":[67],"80%":[68],"newly":[70],"formed":[71],"glycosaminoglycans":[72,83,124,182],"secreted":[74],"medium.":[77,127],"Analysis":[78],"types":[81,122],"revealed":[84],"different":[86],"pattern":[87,209],"for":[88,232],"medium,":[91],"respectively,":[92],"which":[93],"is":[94,187],"subject":[95],"time.":[98],"Heparan":[99],"sulfate":[100,113,118,129,199,202,237,240],"remains":[101],"primarily":[102],"cell-bound":[103],"and,":[104],"therefore,":[105],"has":[106],"low":[108],"fractional":[109],"secretion":[110],"rate.":[111],"Chondroitin":[112],"even":[115],"more":[116,175],"dermatan":[117,198,239],"are":[119,192],"main":[121],"Dermatan":[128],"represents":[130],"about":[131],"60%":[132],"total":[134],"medium":[135],"glycosaminoglycans.":[136],"In":[137],"advanced":[138],"(eighth":[140],"day),":[141],"this":[142,224],"type":[143,226],"becomes":[144],"predominant":[146],"one":[147],"cell":[150,225],"layer.":[151],"The":[152],"reduction":[153],"molecular":[156],"weight":[157],"native":[159],"medium-sulfated":[160],"molecules":[161],"papain":[163],"beta-elimination":[166],"puromycin-induced":[169],"inhibition":[170],"their":[172],"synthesis":[173],"than":[176],"75%":[177],"suggest":[178],"formation":[180],"as":[183],"complex":[184],"proteoglycans.":[185],"It":[186],"concluded":[188],"that":[189,217],"major":[194],"cellular":[195],"source":[196],"chondroitin":[201,236],"connective":[205,243],"tissue.":[206,244],"Since":[207],"proteoglycans":[211],"closely":[215],"resembles":[216],"found":[218],"fibrotic":[221],"matrix,":[223],"might":[227],"be":[228],"pathogenetic":[230],"significance":[231],"accumulation":[234],"cirrhotic":[242]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}